Identification of genes encoding squalestatin S1 biosynthesis and in vitro production of new squalestatin analogues

A gene cluster responsible for the biosynthesis of squalestatin S1 (SQS1, 1) was identified by full genome sequencing of two SQS1-producing ascomycetes: Phoma sp. C2932 and unidentified fungus MF5453. A transformation protocol was established and a subsequent knockout of one PKS gene from the cluster led to loss of SQS1 production and enhanced concentration of an SQS1 precursor. An acyltransferase gene from the cluster was expressed in E. coli and the expressed protein MfM4 shown to be responsible for loading acyl groups from CoA onto the squalestatin core as the final step of biosynthesis. MfM4 appears to have a broad substrate selectivity for its acyl CoA substrate, allowing the in vitro synthesis of novel squalestatins

Phase I/II Archaeological Testing on Fleet Street (18AP111), Cornhill Street (18AP112), and 26 Market Space (18AP109), for the Proposed Fleet and Cornhill Streets Reconstruction Project, Annapolis, Maryland, 2008

From 3/31/08 to 5/30/08 staff from the Department of Anthropology, University of Maryland, College Park (UMCP), Archaeology in Annapolis Project, conducted archaeological testing on city-owned public right-of-ways at 26 Market Space (18AP109), on Fleet Street (18AP111), and on Cornhill Street (18AP112) prior to the upcoming undergrounding and replacement of city-owned utilities along and beneath these streets. In addition, from 06/02/08 to 06/20/08, undergraduate and graduate students enrolled in the University of Maryland, Field School in Urban Archaeology conducted further testing of city-owned public right-of-ways on Cornhill Street (18AP112). This Phase II investigation has been conducted at the request of the City of Annapolis, Department of Public Works (DPW) as part of the Fleet and Cornhill Streets Reconstruction Project. The project area comprises the streetscapes of what is referred to as the Fleet-Cornhill neighborhood. Eleven test units were used to evaluate archaeological integrity and significance of these sites and to evaluate the potential effects of planned construction on archaeological resources. Background research shows that the Fleet Street neighborhood was initially developed in the late 17th and early 18th century. Throughout the later 18th, 19th and 20th centuries the area became known as an ethnically diverse working class neighborhood in the heart of the city. Historical residents of the project area have included in the early 20th century native people of European, African descent, and a community of Russian Ashkenazi Jews in the early 20th century. Previous archaeological investigations found evidence of intact archaeological resources within the project area dating from the 18th to 20th centuries. In addition to providing evidence of patterns of Annapolis’ historical urbanization, several features excavated in the course of this project have shed light on the development of public space within this working class neighborhood. These features include a corduroy or log road dating to the first quarter of the 18th century; what is believed to be a Yoruba ritual bundle dating to the first quarter of the 18th century; and a series of city improvements (i.e. curbs, sidewalks, and a public well) dating from the 18th through 20th centuries. A high degree of archaeological integrity at all three sites has the potential to add considerable knowledge concerning both Annapolis city development, and an ethnically diverse working class community. All three sites are eligible for inclusion in the National Register of Historic Places under Criterion D. Because of the integrity and uniqueness of the archaeological record within the project area, it is recommended that further archaeological research be done. Included within this recommendation is the need to process flotation and macrobotanical samples recovered in the fiel

Phase II Archaeological Testing on Wye Greenhouse (18TA314), Talbot County, Maryland, 2008

From October 27, 2008 to November 24, 2008 staff from the Department of Anthropology, University of Maryland, College Park (UMCP), Archaeology in Annapolis Project, conducted archaeological testing on the Wye House Greenhouse (18TA314), Talbot County, Maryland. This Phase II investigation has been conducted at the request of the Greenhouse’s current owner, Mrs. Mary Tilghman, prior to planned Greenhouse foundation stabilization efforts. The project area for this Phase II archaeological investigation comprises the immediate exterior perimeter of the Wye Greenhouse foundation. Seven test units were excavated in the course of this project to evaluate archaeological integrity and to evaluate the potential effects of planned stabilization efforts on archaeological resources. In addition to questions of archaeological integrity, research questions guiding this project focused on the architectural development of the Wye Greenhouse as well as its social use, both by members of the Lloyd family and the plantation’s enslaved African-American inhabitants. Background historical research and oral histories differ concerning the Greenhouse’s initial date of construction. Historical research suggests a construction date of the c. 1770s, while oral histories suggest an initial date of construction of c. 1740s. Archaeological testing has shown that the Greenhouse underwent two major developmental phases—with the main block of the Greenhouse having been constructed in the 1770s and the East and West Wings and hypocaust system added in the mid 1780s. In addition to providing evidence of the Greenhouse’s structural change, levels and features excavated in the course of this project have shed light on the social use of the Wye Greenhouse throughout the 18th and 19th centuries. Artifact deposits analyzed in this report detail the Lloyd family’s use of the Greenhouse as both a social space and as a symbol of 18th century opulence. Artifact analyses also shed light on the use of the Greenhouse’s north shed as a slave quarter from the 1790s through the 1840s. Testing in the course of this project has concluded that there is a high degree of archaeological integrity within the project’s area of potential effect. In addition, testing has determined that intact archaeological resources have the distinct potential to add a considerable depth of historical knowledge concerning the Greenhouse’s structural change and social use throughout the 18th and 19th centuries. Archaeological evidence detailed in this report should be read as supporting evidence for the Greenhouse’s inclusion on the National Register of Historic Places

Archaeological Excavations on the Long Green (18TA314), 2005-2008, Talbot County, Maryland, 2009

Four site reports are included in the one document. Locus 1: Tulip Poplar slave quarter; Locus 3: the North Building slave quarter; Locus 4: Red Overseer’s House, named by Frederick Douglas, home of Overseer Sevier; and Shovel Test Pits from 2005-2008

Search for R-parity Violating Supersymmetry in Dimuon and Four-Jets Channel

We present results of a search for R-parity-violating decay of the neutralino chi_1^0, taken to be the Lightest Supersymmetric Particle. It is assumed that this decay proceeds through one of the lepton-number violating couplings lambda-prime_2jk (j=1,2; k=1,2,3). This search is based on 77.5 pb-1 of data, collected by the D0 experiment at the Fermilab Tevatron in ppbar collisions at a center of mass energy of 1.8 TeV in 1992-1995.Comment: 10 pages, 3 figure

A Quasi-Model-Independent Search for New Physics at Large Transverse Momentum

We apply a quasi-model-independent strategy ("Sleuth") to search for new high p_T physics in approximately 100 pb^-1 of ppbar collisions at sqrt(s) = 1.8 TeV collected by the DZero experiment during 1992-1996 at the Fermilab Tevatron. Over thirty-two e mu X, W+jets-like, Z+jets-like, and 3(lepton/photon)X exclusive final states are systematically analyzed for hints of physics beyond the standard model. Simultaneous sensitivity to a variety of models predicting new phenomena at the electroweak scale is demonstrated by testing the method on a particular signature in each set of final states. No evidence of new high p_T physics is observed in the course of this search, and we find that 89% of an ensemble of hypothetical similar experimental runs would have produced a final state with a candidate signal more interesting than the most interesting observed in these data.Comment: 28 pages, 17 figures. Submitted to Physical Review

Search for Electroweak Production of Single Top Quarks in ppbar Collisions

We present a search for electroweak production of single top quarks in the electron+jets and muon+jets decay channels. The measurements use ~90 pb^-1 of data from Run 1 of the Fermilab Tevatron collider, collected at 1.8 TeV with the DZero detector between 1992 and 1995. We use events that include a tagging muon, implying the presence of a b jet, to set an upper limit at the 95% confidence level on the cross section for the s-channel process ppbar->tb+X of 39 pb. The upper limit for the t-channel process ppbar->tqb+X is 58 pb.Comment: 11 pages, 2 figures. This is the published versio

Search for Large Extra Dimensions in Dielectron and Diphoton Production

We report a search for effects of large extra spatial dimensions in ppbar collisions at a center-of-mass energy of 1.8 TeV with the DZero detector, using events containing a pair of electrons or photons. The data are in good agreement with the expected background and do not exhibit evidence for large extra dimensions. We set the most restrictive lower limits to date, at the 95% confidence level, on the effective Planck scale between 1.0 TeV and 1.4 TeV for several formalisms and numbers of extra dimensions.Comment: 6 pages, 2 figures, submitted to Phys. Rev. Let

Direct Search for Charged Higgs Bosons in Decays of Top Quarks

We present a search for charged Higgs bosons in decays of pair-produced top quarks in pbar p collisions at sqrt(s) = 1.8 TeV using 62.2 pb^-1 of data recorded by the D0 detector at the Fermilab Tevatron collider. No evidence is found for signal, and we exclude at 95% confidence most regions of the (M higgs, tan beta) parameter space where the decay t->H b has a branching fraction greater than 0.36 and B(H -> tau nu) is large.Comment: 11 pages, 4 figures, submitted to Phys. Rev. Let