Characterisation of Oil Palm Acyl-CoA-Binding Proteins and Correlation of their Gene Expression with Oil Synthesis

Acyl-CoA-binding proteins (ACBPs) are involved in binding and trafficking acyl-CoA esters in eukaryotic cells. ACBPs contain a well-conserved acyl-CoA-binding domain (ACBD). Their various functions have been characterized in the model plant Arabidopsis and, to a lesser extent, in rice. In this study, genome-wide detection and expression analysis of ACBPs were performed on Elaeis guineensis (oil palm), the most important oil crop in the world. Seven E. guineensis ACBPs were identified and classified into four groups according to their deduced amino acid domain organization. Phylogenetic analysis showed conservation of this family with other higher plants. All seven EgACBPs were expressed in most tissues while their differential expression suggests various functions in specific tissues. For example, EgACBP3 had high expression in inflorescences and stalks while ACBP1 showed strong expression in leaves. Because of the importance of E. guineensis as an oil crop, expression of EgACBPs was specifically examined during fruit development. EgACBP3 showed high expression throughout mesocarp development, while EgACBP1 had enhanced expression during rapid oil synthesis. In endosperm, both EgACBP1 and EgACBP3 exhibited increased expression during seed development. These results provide important information for further investigations on the biological functions of EgACBPs in various tissues and, in particular, their roles in oil synthesis

Mapping on slope seepage problem using Electrical Resistivity Imaging (ERI)

The stability of slope may influenced by several factors such as its geomaterial properties, geometry and environmental factors. Problematic slope due to seepage phenomenon will influenced the slope strength thus promoting to its failure. In the past, slope seepage mapping suffer from several limitation due to cost, time and data coverage. Conventional engineering tools to detect or mapped the seepage on slope experienced those problems involving large and high elevation of slope design. As a result, this study introduced geophysical tools for slope seepage mapping based on electrical resistivity method. Two spread lines of electrical resistivity imaging were performed on the slope crest using ABEM SAS 4000 equipment. Data acquisition configuration was based on long and short arrangement, schlumberger array and 2.5 m of equal electrode spacing interval. Raw data obtained from data acquisition was analyzed using RES2DINV software. Both of the resistivity results show that the slope studied consists of three different anomalies representing top soil (200 – 1000 Ωm), perched water (10 – 100 Ωm) and hard/dry layer (> 200 Ωm). It was found that seepage problem on slope studied was derived from perched water zones with electrical resistivity value of 10 – 100 Ωm. Perched water zone has been detected at 6 m depth from the ground level with varying thickness at 5 m and over. Resistivity results have shown some good similarity output with reference to borehole data, geological map and site observation thus verified the resistivity results interpretation. Hence, this study has shown that the electrical resistivity imaging was applicable in slope seepage mapping which consider efficient in term of cost, time, data coverage and sustainability

Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis.

Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (GFP) and cloned into Lactococcus lactis pNZ8048 and pMG36e vectors, respectively. Western blot analysis indicated that the GFP proteins were secreted using both heterologous SPs. ELISA showed that the secretion efficiency of GFP using Spk1 (0.64 μg/ml) was similar to using Usp45 (0.62 μg/ml) and Spk3 (0.58 μg/ml)

The oil palm VIRESCENS gene controls fruit colour and encodes a R2R3-MYB

Oil palm, a plantation crop of major economic importance in Southeast Asia, is the predominant source of edible oil worldwide. We report the identification of the VIRESCENS (VIR) gene, which controls fruit exocarp colour and is an indicator of ripeness. VIR is a R2R3-MYB transcription factor with homology to Lilium LhMYB12 and similarity to Arabidopsis PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1). We identify five independent mutant alleles of VIR in over 400 accessions from sub-Saharan Africa that account for the dominant-negative virescens phenotype. Each mutation results in premature termination of the carboxy-terminal domain of VIR, resembling McClintock's C1-I allele in maize. The abundance of alleles likely reflects cultural practices, by which fruits were venerated for magical and medicinal properties. The identification of VIR will allow selection of the trait at the seed or early-nursery stage, 3-6 years before fruits are produced, greatly advancing introgression into elite breeding material

Business analytics in industry 4.0: a systematic review

Recently, the term “Industry 4.0” has emerged to characterize several Information Technology and Communication (ICT) adoptions in production processes (e.g., Internet-of-Things, implementation of digital production support information technologies). Business Analytics is often used within the Industry 4.0, thus incorporating its data intelligence (e.g., statistical analysis, predictive modelling, optimization) expert system component. In this paper, we perform a Systematic Literature Review (SLR) on the usage of Business Analytics within the Industry 4.0 concept, covering a selection of 169 papers obtained from six major scientific publication sources from 2010 to March 2020. The selected papers were first classified in three major types, namely, Practical Application, Reviews and Framework Proposal. Then, we analysed with more detail the practical application studies which were further divided into three main categories of the Gartner analytical maturity model, Descriptive Analytics, Predictive Analytics and Prescriptive Analytics. In particular, we characterized the distinct analytics studies in terms of the industry application and data context used, impact (in terms of their Technology Readiness Level) and selected data modelling method. Our SLR analysis provides a mapping of how data-based Industry 4.0 expert systems are currently used, disclosing also research gaps and future research opportunities.The work of P. Cortez was supported by FCT - Fundação para a Ciência e Tecnologia within the R&D Units Project Scope: UIDB/00319/2020. We would like to thank to the three anonymous reviewers for their helpful suggestions

Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort

The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microarray comprising various nucleocapsid protein structural motifs, including two structural domains and three intrinsically disordered regions. Quantitative data from the microarray provided complete differentiation between cases and pre-pandemic controls (100% sensitivity and specificity) in a case-control cohort (n = 100). We then assessed the influence of disease severity, age, and ethnicity on the strength and breadth of the humoral response in a multi-ethnic cohort (n = 138). As expected, patients with severe disease showed significantly higher antibody titers and interestingly also had significantly broader epitope coverage. A significant increase in antibody titer and epitope coverage was observed with increasing age, in both mild and severe disease, which is promising for vaccine efficacy in older individuals. Additionally, we observed significant differences in the breadth and strength of the humoral immune response in relation to ethnicity, which may reflect differences in genetic and lifestyle factors. Furthermore, our data enabled localization of the immuno-dominant epitope to the C-terminal structural domain of the viral nucleocapsid protein in two independent cohorts. Overall, we have designed, validated, and tested an advanced serological assay that enables accurate quantitation of the humoral response post natural infection and that has revealed unexpected differences in the magnitude and epitope utilization within a population

A Keplerian disk with a four-arm spiral birthing an episodically accreting high-mass protostar

High-mass protostars (M⋆ > 8M⊙) are thought to gain the majority of their mass via short, intense bursts of growth. This episodic accretion is thought to be facilitated by gravitationally unstable and subsequently inhomogeneous accretion disks. Limitations of observational capabilities, paired with a lack of observed accretion burst events, have withheld affirmative confirmation of the association between disk accretion, instability and the accretion burst phenomenon in high-mass protostars. Following its 2019 accretion burst, a heatwave driven by a burst of radiation propagated outward from the high-mass protostar G358.93-0.03-MM1. Six very long baseline interferometry observations of the radiatively pumped 6.7 GHz methanol maser were conducted during this period, tracing ever increasing disk radii as the heatwave propagated outward. Concatenating the very long baseline interferometry maps provided a sparsely sampled, milliarcsecond view of the spatio-kinematics of the accretion disk covering a physical range of ~50–900 AU. We term this observational approach ‘heatwave mapping’. We report the discovery of a Keplerian accretion disk with a spatially resolved four-arm spiral pattern around G358.93-0.03-MM1. This result positively implicates disk accretion and spiral arm instabilities into the episodic accretion high-mass star formation paradigm