FEATURE EXTRACTION OF LONG BONE (TIBIA) USING TWIST KERNEL INVARIANT DISPARITIES(TKID)

Orthopaedic treatment requires proper classification of bone fractures. This helps doctors to assess the injury and plan the treatment accordingly. Different bones have different shapes, sizes and features. Therefore, fractures vary depending on the type, location and pattern of the bone.. Before classification accurate feature extraction is most important to improve the accuracy. This research proposed a new Twist Kernel Invariant Disparities(TKID) method to extract features after segmentation. This research extracts the following features are identified from this segmented image: Mean, SD, Entropy, RMS, Variance, Smoothness, Kurtosis, Skewness, IDM, Contrast, Correlation, Energy, Homogeneity, colour, size, shape, dimensions, convex and concav

A Awareness And Knowledge On Comparative Cytotoxic Effect Of Hibiscus- Marigold Herbal Formulation And Its Mediated Silver Nanoparticles

Introduction: In recent years, there has been a resurgence in the use of traditional medicinal herbs, and as a result, pharmaceutical companies are investing heavily in developing natural medications derived from plants. Nanoparticles act in a number of potential ways and fields. Chemical synthesis of nanoparticles is no longer advantageous compared to plant-based synthesis. The current study uses a green method to create silver nanoparticles (AgNPs) utilizing flower extract. Materials and methods: The cytotoxic activity for gel was tested by the following convention proposed by Muzushima and Kabayashi with specific alterations. 0.05 mL of hibiscus and marigold of various fixations. Result: Using rosa and jasminum extract in the manufacture of silver nanoparticles, the nanoparticles showed remarkable antioxidant and anti-inflammatory activity. It was discovered that the cytotoxic effect was less harmful, demonstrating biocompatibility. Rosa jasminum extract of AgNps showed good anti-inflammatory efficacy in the EA and BSA assays. Conclusion: From our study we concluded that Silver nanoparticles were created using rosa and jasminum extract, and the nanoparticles displayed impressive cytotoxicity

Partial Purification of Extracellular Amylase From Halotolerant Actinomycetes Streptomyces brasiliensis MML2028

Amylase is considered as an industrially important enzyme as it occupies the most important function in the food, paper, and pharmaceutical industries. The present study is concerned with the optimization, production and partial purification of halotolerant amylase from newly isolated Streptomyces brasiliensis MML2028, from Kelambakkam salt pan, Tamil Nadu, India. The primary screening was carried out by well diffusion assay to find the zone of lysis. The assay was observed for each media optimization by measuring the release of reducing sugar (RS) by the 3,5 dinitro salicylic acid (DNS) method and expressed in the international unit (UI). Ammonium sulphate precipitation was used to partially purify the enzyme and then lyophilized. SDS-PAGE was performed to identify the molecular weight. The production medium was optimized with 1% of the starch substrate, 3% of NaCl at 24˚C and pH 9, and incubation of 9 days. The total activity of the partially purified α-amylase was observed to be 1806.9U/mL. The partially purified enzyme was more active with 3% NaCl, pH 8, and 24˚C which is known to be a halotolerant alkaline α-amylase. The enzyme showed tolerance towards magnesium, manganese ions, Triton x-100, and urea. De-inking of α-amylase showed good results proving that the enzyme activity is more efficient. Hence, the alkaliphilic amylase from Halotolerant actinomycetes S. Brasiliensis MML2028 could be a better microbial source that can be used in many industries, especially in paper and textiles

A Genome-Wide Association Study Provides New Evidence That CACNA1C Gene is Associated With Diabetic Cataract

PURPOSE: Diabetic cataract is one of the major eye complications of diabetes. It was reported that cataract occurs two to five times more frequently in patients with diabetes compared with those with no diabetes. The purpose of this study was to identify genetic contributors of diabetic cataract based on a genome-wide association approach using a well-defined Scottish diabetic cohort. METHODS: We adapted linked e-health records to define diabetic cataract. A diabetic cataract case in this study was defined as a type 2 diabetic patient who has ever been recorded in the linked e-health records to have cataracts in both eyes or who had previous cataract extraction surgeries in at least one eye. A control in this study was defined as a type 2 diabetic individual who has never been diagnosed as cataract in the linked e-health records and had no history of cataract surgeries. A standard genome-wide association approach was applied. RESULTS: Overall, we have 2341 diabetic cataract cases and 2878 controls in the genetics of diabetes audit and research in Tayside Scotland (GoDARTS) dataset. We found that the P value of rs2283290 in the CACNA1C gene was 8.81 × 10(−10), which has reached genome-wide significance. We also identified that the blood calcium level was statistically different between diabetic cataract cases and controls. CONCLUSIONS: We identified supporting evidence that CACNA1C gene is associated with diabetic cataract. The role of calcium in the cataractogenesis needs to be reevaluated in future studies

Effect of Selected Fruit Wastes on Hematological Parameters in DEN-Induced Hepatic Carcinoma in Rats

Fruits and processed food demand has greatly increased due to which a huge amount of fruit waste is generated. In this study, the effect of the ethanolic extract of waste material Punica granatum (pomegranate) peel and Vitis vinifera (grapes) seeds was evaluated on hematological parameters in Wistar rats with diethylnitrosamine (DEN) induced hepatocellular carcinoma. The treatments were divided into four groups: Group 1 was untreated while Groups 2-4 received 200 mg/kg body weight of DEN by single intraperitoneal administration. Groups 3 and 4 received DEN and co-treated with 400 mg/kg pomegranate peel extract and 400 mg/kg grape seed extract, respectively. There was a significant decrease in the body weight of animals in Group 2, while Group 3 and 4 animals were found to have a significant rise in body weight. The weight of the liver was significantly increased in the cancer-bearing group and its size was significantly reverted in the treated groups. The kidney and spleen showed a significant decrease in size of cancer-induced groups, and these organs significantly increased in treated groups. Hemoglobin, red blood cells (RBC), neutrophil, packed cell volume (PCV), Mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) were significantly reduced in Group 2 animals compared to Group 1. The levels of white blood cells (WBC) and erythrocyte sedimentation rate were increased significantly in Group 2 animals relating to Group 1. Both in Group 3 and 4 animals, these changes were reversed. Results reveal that selected fruit wastes alleviate vital hematological parameters in hepatocellular carcinoma-induced rats