Thermal Ground Testing of Concorde and Veras or Improvement in French Test Methods and Facilities

The more numerous the requirements are in aerospace vehicle operations, the more the ground test facility have to improve both the nature and the quality of the equipment as well as the volume and the size of the sites, for the importance of ground testing cannot be over emphasized. As an example, two ground test programs are reviewed in this presentation, namely CONCORDE and VERAS. Special emphasis is given to the facility implementations due respectively to the static and fatigue test of CONCORDE in heat environment and to the dynamic test of VERAS in high level and transient temperature conditions

A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola

Background Flavin-dependent monooxygenases are involved in key biological processes as they catalyze a wide variety of chemo-, regio- and enantioselective oxygenation reactions. Flavoprotein monooxygenases are frequently encountered in micro-organisms, most of which require further functional and biocatalytic assessment. Here we investigated the function of the AbMak1 gene, which encodes a group A flavin monooxygenase in the plant pathogenic fungus Alternaria brassicicola, by generating a deficient mutant and examining its phenotype. Results Functional analysis indicates that the AbMak1 protein is involved in cell wall biogenesis and influences the melanization process. We documented a significant decrease in melanin content in the Δabmak1 strain compared to the wild-type and complemented strains. We investigated the cell wall morphology and physical properties in the wild-type and transformants using electron and atomic force microscopy. These approaches confirmed the aberrant morphology of the conidial wall structure in the Δabmak1 strain which had an impact on hydrophilic adhesion and conidial surface stiffness. However, there was no significant impairment in growth, conidia formation, pathogenicity or susceptibility to various environmental stresses in the Δabmak1 strain. Conclusion This study sheds new light on the function of a fungal flavin-dependent monooxygenase, which plays an important role in melanization

Early-onset Behr syndrome due to compound heterozygous mutations in OPA1

International audienceNo abstract availabl