すり身加工品で感知される塩味強度とテクスチャーの関係に関する研究

東京海洋大学博士学位論文 平成29年度(2017) 応用生命科学 課程博士 甲第447号指導教員: 岡﨑惠美子東京海洋大学201

An Unconventional Pathway: A Qualitative Inquiry Into the Experience of Racial Minority Transfer Students

Transfer students’ success is increasingly being discussed in higher education. A large population of transfer students includes racial minority, low-income, and first-generation college students. Due to the multiple layers of students’ identities, it has been difficult for educational leaders in institutions to grasp the essential elements needed to serve these students. As a result, this qualitative study contributes to this discussion and efforts to support the transfer student’s success. In this study, qualitative methods, including interviews with 14 students from 4-year universities and community colleges and a survey, were used to identify the steps and resources associated with supporting transfer students. The critical race theory framework served as the central lens to determine how racial factors played a vital key in the students’ post-transfer experience. The goal of this study was to ensure that the transfer students receive the essential resources and guidance from their institutions to have a successful transfer pathway to earning their degree

Optimization of Acetone-Butanol-Ethanol Production from Cassava Starch by a Mixed Culture of Clostridium sp. and Bacillus sp. in Batch and Fed-batch Fermentation

Thesis (Ph.D., Biotechnology)--Prince of Songkla University, 200

THE SITUATION OF MENTAL HEALTH OF VIETNAM DISABLED ATHLETES IN WEIGHTLIFTING AND JAVELIN BEFORE PARALYMPIC GAMES 2021

The purpose of this study was to find out the situation of Vietnam disabled athletes' mental health before Paralympic Games 2021. Seven athletes from the Vietnam disabled team (including 4 male and 3 female athletes in weightlifting and javelin contents) were chosen to identify their mental health before Paralympic Games. The International Olympic Committee's (IOC) Sports Metal Health Assessment Tool 1 (SMHAT-1) was used to determine the risk or experience of symptoms and mental health disorders of national team athletes with disabilities, facilitating timely referral for athletes in need of adequate support and treatment. The study identified the mental health of disabled Vietnam athletes in weightlifting and javelin. Thus, it led the management and coaches to have a specific plan for the appropriate pre-competition preparation for athletes, thereby getting good results in the upcoming Paralympic Games 2021.  Article visualizations

Assay of Vitamin B6 (Pyridoxine Hydrochloride) Utilizing Isocratic Reversed Phase High Performance Liquid Chromatography

Aims: To demonstrate an analysis for vitamin B6 from commercial aqueous nutritional drinks and solid tablets, utilizing isocratic conditions with high performance liquid chromatography (HPLC) and UV detection at 290 nm. Study Design: Vitamin B6 in the form of pyridoxine hydrochloride is assayed by HPLC from various samples. Place and Duration of Study: Department of Chemistry, Durham Science Center, University of Nebraska, Omaha Nebraska from May to August 2016. Methodology: Utilizing a reversed-phase C-18 column with eluent solvent (19% ethanol, 77% water, 4% acetic acid), the samples were prepared in sample solvent (19% ethanol, 81% distilled water). Detection of vitamin B6 was accomplished at 290 nm. Analysis of samples was done following solubilizing in aqueous conditions having ethanol at 10% to 20% (v/v). Column pressure at 1900 psig, rise time 0.1 with flow rate 1.0 mL/minute. Elution peak for vitamin B6 occurred consistently at 1.6 minutes. Nutritional samples, aqueous samples, and solid pills were prepared in aqueous solvent with various levels of ethanol. Results: Levels of vitamin B6 detected were as low as 4.4029x10-5 molar to 7.8081x10-4 molar. Sensitivity for vitamin B6 was highest at 290 nm. Reverse phase isocratic conditions is shown to be effective for determination of vitamin B6 in aqueous based samples. Standard curves applied are highly linear in range from zero to 7.8081x10-4 molar (y = 112,521,145.5x + 2,818.6), having coefficient of determination (R2 = 0.9948) with very strong positive correlation coefficient (r= 0.9974). Percent recovery of vitamin ranged from 95% to 105%. Amounts of vitamin present in drinks from same manufacturer were consistent. Conclusion: Utilizing reversed phase column, isocratic solvent conditions with ethanol in water, and a UV detector set at 290 nm is effective for determination of vitamin B6. Ethanol-water solvent system is effective. Vitamin B6 was found in various amounts in nutritional drinks tested. Percent recovery of vitamin averaged 101% with a standard deviation of 2.4%. Pyridoxine hydrochloride was effectively assayed from aqueous samples, vitamin preparations, and vitamin tablets. The methodology presented in this study will be useful for quality control analysis for commercial production. Analysis methods for vitamin assay are a necessary objective to ensure quality control of commercial products and medicinal applications

Adaptation options for agricultural cultivation systems in the South Central Coast under the context of climate change: Assessment Report.

This report highlights the results of consultation meetings and field visits organized by the Department of Crop Production and the CGIAR Research Program on Climate Change, Agriculture and Food Security in Southeast Asia in association with the three offices of the Department of Agriculture and Rural Development in the South Central Coast provinces of Binh Thuan, Ninh Thuan, and Khanh Hoa, in combination with consultation with the provinces in the conference: “Summing up crops production in the Winter-Spring season in 2018-2019, implementing the Summer-Autumn season, Main rice season in 2019 for the South Central Coast and the Central Highlands” held by the Ministry of Agriculture and Rural Development in Tam Ky City, Quang Nam Province on 12 April 2019. The meetings underlined the progress made by the provinces on climate change adaptation and mitigation, options for risk reductions in agricultural production, and conversion of crop structure as results of implementing the guidelines of the provinces and the Sector, especially, solutions for reservation and efficient and economic use of water under the context of climate change. This assessment report also reviews some issues related to the agricultural transformation of the region in adapting to risks caused by climate change. They are based on comparative advantages in terms of geographical location and market of key agricultural products. This report also points out shortcomings in using land and unreasonable points in managing and using important natural resources, especially water, and provides recommendations for the agricultural transformation and inter-regional connection with the Central Highlands and the Southeast. The team also introduces climate-related risks maps and adaptation plans (CS MAP) which is applied in the five provinces in the Mekong Delta Region, and hopes this solution’s expansion shall be supported by the Ministry of Agriculture and Rural Development and the provinces

Differentiation Effect of Two Alkaloid Fractions from Vietnamese Lycopodiaceae on Mouse Neural Stem Cells

Various Lycopodium alkaloids have been studied for their various biological activities including anti-inflammatory, antioxidant, immunomodulatory, and neuroprotective activities. Moreover, these alkaloid compounds have high potential in the treatment of neuron degenerative disease. This study has been carried out to test the effect of Huperzia serrata (Thunb.) Trevis, and Lycopodium clavatum L alkaloid fractions on the mouse neural stem cells (NSCs). Firstly, the alkaloid fractions were used to verify its toxicity on NSCs. The multiple concentrations of alkaloid fractions from H. serrata (0.044; 0.088; 0.175; 0.35; 0.7; 1.4 mg/ml) and L. clavatum (0.031; 0.063; 0.125; 0.25; 0.50; 1.0; 2.0 mg/ml) have been used for the treatment of NSCs at period of 48h incubation. Results of the study suggested that the IC50 value of H. serrata and L. clavatum was 0.56 mg/ml and 0.50 mg/ml, respectively. Then, the NSCs were differentiated in the presence of 5 and 10 µg/ml of alkaloid fraction from H. serrata; 0.625 and 1.25 µg/ml of alkaloid fraction from L. clavatum for 6 days. Here, we observed the primary NSCs treated with alkaloid fraction extract from H. serrata showed the increased gene expression level of early neuron TUBB3 and neuron-specific cytoskeleton MAP2. On the other hand, the L. clavatum alkaloid fraction increased the expression of neural stem cell marker genes (Nestin and PAX6) and decreased neuron marker genes. In conclusion, these results established that alkaloid fraction from H. serrata promoted differentiation of the mouse NSCs to neuron cells, and L. clavatum extract had a capacity for stemness maintenance

Developing a zebrafish-based pipeline to investigate the biological properties of medicinal plant extracts from Northern Vietnam

peer reviewe

Belgium

peer reviewedPurpose: Traditional medicines have been used in Vietnam for thousands of years, and still play an essential role in healthcare. However, so far, there is no clear definition of circumstances in which plants can be collected, conserved, and extracted; viable models for a productive and sustainable exploitation of natural extracts are missing, sometimes leading to severe and unfavorable consequences on health. In this study, we aim to develop a cost-effective pipeline to assess the pharmaco-/toxicological properties of these medicinal materials using the zebrafish embryo as an in vivo model. Methods: Medicinal plants extracts were prepared by incorporating in the design more standardized methods as the traditional ones, using increasing polarity solvents. After that, the zebrafish has been chosen as a whole animal model to determine the core of the biological properties of a specific extract. Embryotoxicity was analyzed by exposing extracts to zebrafish embryos, which were monitored at specific times during 4 days for survival and the presence of developmental malformations. In addition, the zebrafish transgenic line Tg(fli1:EGFP) displaying fluorescent endothelial cells was used to visualize blood vessels and detect effects on angiogenesis. Proof-of-concept experiments were done by passing samples from two plants: Caesalpinia sappan L. and Glochidion eriocarpum to evaluate its efficiency. Results: Effects of C. sappan L. extract on zebrafish embryonic morphology and lethality were determined in the corresponding concentration-response curves (LC50=500 mg/L and EC50=40 mg/L). Analysis of transgenic zebrafish showed the anti-angiogenic properties in a dose dependent manner. In addition, the interaction between this plant extract and the embryo chorion was explored. On the other hand, G. eriocarpum extract exposure led to a biphasic response from the zebrafish embryos, suggesting a protective role of the embryonic chorion. Our results may contribute to the design of a rational high throughput pharmacological screening approach using the zebrafish model