The Effects of Native Plant Species Vegetated in Constructed Wetland on Removal of Selected Pollutants in Water of Bomachoge Sub County in Kisii County-Kenya

The relationship between water quality and health should be appreciated by engineers and researchers so that water resources users can be sensitive to water contamination and associated health impacts. It’s against this background that this study was carried out to establish the quality of water before and after passing through vegetated constructed wetlands. This is presumably a less costly method of naturally reducing pollutant load in water sources. This study evaluated the levels of microbial and chemical parameters of water in surface flow through constructed wetlands vegetated with Colocasia esculenta, Cyperus esculentus and their combination to determine the efficacy of plant species in removal of these parameters. The study was conducted from August 2015 to February 2016 at Bomachoge Sub County of Kisii County, Kenya. Measurements and laboratory examinations of water samples was carefully done and results revealed that there was significant (p<0.005) removal of all selected parameters, whereby Colocasia esculenta was more effective than Cyperus esculentus and their mixture. C. esculenta (A), C.esculentus (B) and their combination (AB) removed selected  bacteriological parameters such as faecal coliforms by (A 98%, B 16%, AB 36%), faecal Streptococcus (A 100%, B 13%, AB 15%), total coliforms (A 98%, B 18%, AB 26%) and Escherechia coli (A 97%, B 11%, AB 34%) and physico-chemical parameters such as, dissolved oxygen increased by (A-244%, B-318%, AB -107%) conductivity removed by (A 65%, B 41%, AB -28%), phosphates (A 95%, B -20%, AB 37%), nitrates (A 86%, B -38%, AB 40%) and turbidity (A 65%, B 67% AB -19%)  from the result DO increased significantly. These results show that C. esculenta performance was higher compared to C.esculentus and the mixture of both plants, however, the combination of two plant species performed better than C. esculentus. Statistica statistical program was used to compare the overall, among and within samples. From the findings it shows that both plant species significantly removed pollutants from water. It was recommended that C.esculenta and C.esculentus plant species to be conserved and more studies to be done on other native species in other places of kisii county to ascertain their efficacy in water purification. Keywords: Faecal, Contamination, Anova, Parameters, Coliforms, Wetland, Bacteriological

An Inventory of Medicinal Plants used to Treat Gynaecological-Obstetric-Urino-Genital Disorders in South Nandi Sub County in Kenya

This survey aims at identifying plants that may contribute to the identification and development of new drugs.An ethnomedicinal survey was conducted among the communities in Aldai Division, South Nandi Sub County, Kenya. A total of 56 plants were documented with 30 families are included. The majority of species belong to the families namely Euphorbiaceae, Lamiaceae, Apocynaceae and Fabaceae. Over half of all plants recorded are used treat infections, while ¼ for used labour-child birth and copulation disorders. Keywords: Ethnomedicine, gynaecological-obstetric-urinary, medicinal plants, Nandi

Occurrence of Aspergillus species and aflatoxin contamination in raw and roasted peanuts from formal and informal markets in Eldoret and Kericho towns, Kenya

Published Online: August 2013.The population and diversity of fungal species and levels of aflatoxin contamination were investigated in 228 marketed peanut samples; 140 from formal and 88 from informal markets, in Kericho and Eldoret towns of Kenya. Ground pea- nut samples were cultured on Modified Dichloran Rose Bengal (MDRB) agar while aflatoxin level was quantified based on indirect competitive ELISA. Correlation between the incidence of major aflatoxin-producing fungal species and aflatoxin levels was also established. Fungal species commonly isolated from the peanut samples included Asper-gillus flavus L strain, A. flavus S strain, A. parasiticus, A. tamarii, A. caelatus, A. alliaceus (all of Aspergillus section Flavi) and A. niger. Fungi isolated in low frequency included Fusarium spp., Penicillium spp., Mucor spp. and Rhi- zopus spp. Aflatoxin levels in peanut products ranged from 0 to 2345 μg/kg in raw peanuts, 0 to 382 μg/kg in roasted coated peanuts, and 0 to 201 μg/kg in roasted de-coated peanuts. Overall, levels of total aflatoxin were higher in sam- ples from informal (mean = 97.1 μg/kg) than formal (mean = 55.5 μg/kg) market outlets. There was a positive and sig- nificant correlation (R2 = 0.63; p ≤ 0.05) between aflatoxin levels and the major aflatoxin producing fungi in raw pea- nuts from formal markets in Eldoret town. Additionally, total aflatoxin in raw peanut samples from informal markets in Kericho was positively and significantly correlated (R2 = 0.81; p ≤ 0.05) to the population of A. flavus (L and S strains). In roasted coated peanuts sampled from formal market outlets in Eldoret, aflatoxin levels correlated positively and sig- nificantly (R2 = 0.37; p ≤ 0.05) with A. flavus S strain. There is need to create awareness among peanut traders and con- sumers on proper handling of peanuts and health risks associated with consumption of unsafe peanut products

Association of Body Mass Index, Waist-Hip Ratio, Zinc, Copper, CD4+ T Cells Count and Viral Load in Early HIV Infection in ART Naïve HIV Infected Adults in Taita Taveta County, Kenya

During HIV/AIDS micronutrients play a key role in the host defense systems-Micronutrients such as zinc and copper have been implicated to play important roles in immuno-physiologic functions. The aim of the study was to assess the level of zinc and copper among ART naïve HIV positive adults and assess the association with their waist-hip ratio (WHR), body mass index (BMI), viral load and immunity. Descriptive cross-sectional study was conducted in the voluntary counselling and testing centre (VCT) at Comprehensive Care Clinics  in Taita-Taveta County health care services, Coast, Kenya, blood samples obtained from 192 HIV sero-positive individuals, 18 years of age and gender matched healthy controls were analyzed for zinc and copper using atomic absorption spectrophotometer machine. Data were analysed by statistical (SPSS version 21) computer software. The (mean+ SD) of serum copper and zinc were (158.7+51.0µ/dl, 84.3+51.1µ/dl) in patients and (130.5+17.9 µ/dl, 100.3+5.5 µ/dl) in control group, respectively. Serum zinc level was significantly decreased in HIV patients (P value, 0.05) while serum copper level was significantly increased compared to control group. There was significant association between viral load and waist-hip ratio but not body mass index (P < 0.05). Both body mass index and waist-hip ratio were not significantly associated with CD4+T cells in participants at early HIV infection.Serum zinc was significantly associated to waist-hip ratio in participants in early HIV infection. (P < 0.05). This study indicates that zinc and copper levels are altered in patients in early HIV infection with more decreased Zinc level suspected with increased duration of the HIV infection. It also demonstrates that the associated characteristics in early HIV infection are different from studies in late stages. In conclusion, waist-hip ratio has been identified as a more sensitive predictor and nutrition status maker than body mass index in early HIV hence can be a decisive benchmark for monitoring HIV progression.

Occurrence of Aspergillus Species and Aflatoxin Contamination in Raw and Roasted Peanuts from Formal and Informal Markets in Eldoret and Kericho Towns, Kenya

The population and diversity of fungal species and levels of aflatoxin contamination were investigated in 228 marketed peanut samples; 140 from formal and 88 from informal markets, in Kericho and Eldoret towns of Kenya. Ground peanut samples were cultured on Modified Dichloran Rose Bengal (MDRB) agar while aflatoxin level was quantified based on indirect competitive ELISA. Correlation between the incidence of major aflatoxin-producing fungal species and aflatoxin levels was also established. Fungal species commonly isolated from the peanut samples included Aspergillus flavus L strain, A. flavus S strain, A. parasiticus, A. tamarii, A. caelatus, A. alliaceus (all of Aspergillus section Flavi) and A. niger. Fungi isolated in low frequency included Fusarium spp., Penicillium spp., Mucor spp. and Rhizopus spp. Aflatoxin levels in peanut products ranged from 0 to 2345 μg/kg in raw peanuts, 0 to 382 μg/kg in roasted coated peanuts, and 0 to 201 μg/kg in roasted de-coated peanuts. Overall, levels of total aflatoxin were higher in samples from informal (mean = 97.1 μg/kg) than formal (mean = 55.5 μg/kg) market outlets. There was a positive and significant correlation (R2 = 0.63; p ≤ 0.05) between aflatoxin levels and the major aflatoxin producing fungi in raw peanuts from formal markets in Eldoret town. Additionally, total aflatoxin in raw peanut samples from informal markets in Kericho was positively and significantly correlated (R2 = 0.81; p ≤ 0.05) to the population of A. flavus (L and S strains). In roasted coated peanuts sampled from formal market outlets in Eldoret, aflatoxin levels correlated positively and significantly (R2 = 0.37; p ≤ 0.05) with A. flavus S strain. There is need to create awareness among peanut traders and consumers on proper handling of peanuts and health risks associated with consumption of unsafe peanut products

Preliminary outcomes of a paediatric highly active antiretroviral therapy cohort from KwaZulu-Natal, South Africa

BACKGROUND: Few studies address the use of paediatric highly active antiretroviral therapy (HAART) in Africa. METHODS: We performed a retrospective cohort study to investigate preliminary outcomes of all children eligible for HAART at Sinikithemba HIV/AIDS clinic in KwaZulu-Natal, South Africa. Immunologic, virologic, clinical, mortality, primary caregiver, and psychosocial variables were collected and analyzed. RESULTS: From August 31, 2003 until October 31, 2005, 151 children initiated HAART. The median age at HAART initiation was 5.7 years (range 0.3–15.4). Median follow-up time of the cohort after HAART initiation was 8 months (IQR 3.5–13.5). The median change in CD4% from baseline (p < 0.001) was 10.2 (IQR 5.0–13.8) at 6 months (n = 90), and 16.2 (IQR 9.6–20.3) at 12 months (n = 59). Viral loads (VLs) were available for 100 children at 6 months of which 84% had HIV-1 RNA levels ≤ 50 copies/mL. At 12 months, 80.3% (n = 61) had undetectable VLs. Sixty-five out of 88 children (73.8%) reported a significant increase (p < 0.001) in weight after the first month. Eighty-nine percent of the cohort (n = 132) reported ≤ 2 missed doses during any given treatment month (> 95%adherence). Seventeen patients (11.3%) had a regimen change; two (1.3%) were due to antiretroviral toxicity. The Kaplan-Meier one year survival estimate was 90.9% (95%confidence interval (CI) 84.8–94.6). Thirteen children died during follow-up (8.6%), one changed service provider, and no children were lost to follow-up. All 13 deaths occurred in children with advanced HIV disease within 5 months of treatment initiation. In multivariate analysis of baseline variables against mortality using Cox proportional-hazards model, chronic gastroenteritis was associated with death [hazard ratio (HR), 12.34; 95%CI, 1.27–119.71) and an HIV-positive primary caregiver was found to be protective against mortality [HR, 0.12; 95%CI, 0.02–0.88). Age, orphanhood, baseline CD4%, and hemoglobin were not predicators of mortality in our cohort. Fifty-two percent of the cohort had at least one HIV-positive primary caregiver, and 38.4% had at least one primary caregiver also on HAART at Sinikithemba clinic. CONCLUSION: This report suggests that paediatric HAART can be effective despite the challenges of a resource-limited setting

A Randomized Cross-over Air Filtration Intervention Trial for Reducing Cardiovascular Health Risks in Residents of Public Housing near a Highway

Exposure to traffic-generated ultrafine particles (UFP; particles &lt;100 nm) is likely a risk factor for cardiovascular disease. We conducted a trial of high-efficiency particulate arrestance (HEPA) filtration in public housing near a highway. Twenty residents in 19 apartments living &lt;200 m from the highway participated in a randomized, double-blind crossover trial. A HEPA filter unit and a particle counter (measuring particle number concentration (PNC), a proxy for UFP) were installed in living rooms. Participants were exposed to filtered air for 21 days and unfiltered air for 21 days. Blood samples were collected and blood pressure measured at days 0, 21 and 42 after a 12-hour fasting period. Plasma was analyzed for high sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), tumor necrosis factor alpha-receptor II (TNF-RII) and fibrinogen. PNC reductions ranging from 21% to 68% were recorded in 15 of the apartments. We observed no significant differences in blood pressure or three of the four biomarkers (hsCRP, fibrinogen, and TNF-RII) measured in participants after 21-day exposure to HEPA-filtered air compared to measurements after 21-day exposure to sham-filtered air. In contrast, IL-6 concentrations were significantly higher following HEPA filtration (0.668 pg/mL; CI = 0.465–0.959) compared to sham filtration. Likewise, PNC adjusted for time activity were associated with increasing IL-6 in 14- and 21-day moving averages, and PNC was associated with decreasing blood pressure in Lags 0, 1 and 2, and in a 3-day moving average. These negative associations were unexpected and could be due to a combination of factors including exposure misclassification, unsuccessful randomization (i.e., IL-6 and use of anti-inflammatory medicines), or uncontrolled confounding. Studies with greater reduction in UFP levels and larger sample sizes are needed. There also needs to be more complete assessment of resident time activity and of outdoor vs. indoor source contributions to UFP exposure. HEPA filtration remains a promising, but not fully realized intervention