Application of kaolin and italian natural chabasite-rich zeolitite to mitigate the effect of global warming in vitis vinifera l. Cv. sangiovese

High temperatures and the anomalous distribution of rainfall during the growing season may have a negative impact on grapevine yield and berry composition. In recent years, many studies have focused on the application of agronomical techniques to reduce the negative impact of heat waves on secondary metabolites such as phenols. In particular, treatments with kaolin have shown positive effects on reducing canopy temperatures, enhancing the accumulation of anthocyanins. In regard to the above, three treatments were evaluated: untreated control (C), kaolin (CAO), and chabasite-rich zeolitites (ZEO) applications on cv. Sangiovese in order to verify the cooling effects on leaves and bunches, and the impact on gas exchange, yield parameters, berry composition, and on both chemical and sensory notes of wine. Minerals were sprayed twice around the veraison on the entire canopy at a 3% concentration. The results showed that the application of the minerals was able to reduce the berry temperatures in both years of the trial as compared to the untreated control (C), without affecting vine gas exchange, yield, and soluble solid accumulation. Furthermore, the cooling effect determined an increase in anthocyanin on both the grapes and the wine. At testing, CAO and ZEO wines stood out regarding greater color intensity and were preferred by the judges

Comparison of pure and mixed gas permeation of the highly fluorinated polymer of intrinsic microporosity PIM-2 under dry and humid conditions: Experiment and modelling

This manuscript describes the gas separation performance of PIM-2, a partially fluorinated linear copolymer synthesized from 5,5',6,6'-tetrahydroxy-3,3,3',3'-tetramethylspirobisindane (TTSBI) and decafluorobiphenyl (DFBP). As one of the early members of the family of polymers of intrinsic microporosity, it had never been tested as a gas separation membrane because of insufficient mechanical resistance. This has been solved only recently, allowing the preparation of robust self-standing films. Molecular modelling studies demonstrated a high fractional free volume (34%) and an elevated surface area (642 m2 g-1), and the latter is in good agreement with experimental BET results. Pure gas permeabilities measured on a fixed-volume time-lag instrument at 1 bar compare well with the results of mixed separation tests on a variable volume setup from 1-6 bar(a). Molecular modelling and independent sorption measurements on a gravimetric sorption balance both show strong dual-mode sorption behaviour, especially for CO2 and to a lesser extent for CH4. Temperature-dependent pure gas permeation measurements show typical Arrhenius behaviour, with a clear increase in the activation energy for diffusion with the increasing molecular size of the gas, indicating high size-selectivity. This is in agreement with the highly rigid PIM structure, determined by AFM force spectroscopy measurements. The dual-mode behaviour results in a moderate pressure dependence of the CO2 permeability and the CO2/N2 and CO2/CH4 selectivity, all slightly decreasing with increasing pressure. The presence of humidity in the gas stream has a remarkable small effect on the membrane performance, which is probably due to the high fluorine content and the consequently low water vapour solubility in the polymer, as confirmed by gravimetric sorption measurements. The manuscript describes an extensive study on the structure-property relationships in PIM-2. © 2019 Elsevier B.V.European Commission, EC Grantová Agentura Ceské Republiky, GA Ä?R: 18-05484S --Research on biogas upgrading presented in this work was supported by EU structural funding in the frame of Operational Programme Research, Development and Education, project No. CZ.02.1.01./0.0/0.0/17_049/0008419 “COOPERATION”. This work was further supported by the CNR-CAS bilateral agreement 2016–2018 “Innovative polymeric membranes for pervaporation and advanced gas and vapour separations” and by the Czech Science Foundation (grant no. 18-05484S ). Appendix A -

DC and transient current distribution analysis from self-field measurements on ITER PFIS conductor

Current reconstruction in cable-in-conduit conductors (CICC) cables is a crucial issue to determine cables performance in working conditions, and must be performed using inverse problem approaches as direct measurement is not feasible. The current distribution has been studied for the ITER Poloidal Field Insert Sample (PFIS) conductor using annular arrays of Hall probes placed in three different locations along the sample during the test campaign at the SULTAN facility. The measurement apparatus is also described in the paper, together with the approach to current reconstruction

Detection of infliximab, adalimumab, and anti-drug antibodies: Development and validation of new monotest, automated assays on multiparametric instrument

Objective: To convert manual ELISA kits to fully automated immunoassays that quantify serum drug levels and anti-drug antibodies levels of infliximab and adalimumab (CHORUS Promonitor kits). Desing and methods: CHORUS Promonitor INFLIXIMAB, CHORUS Promonitor ADALIMUMAB, CHORUS Promonitor ANTI-INFLIXIMAB, and CHORUS Promonitor ANTI-ADALIMUMAB kits were compared with the corresponding Promonitor kits to determine sensitivity and specificity of the assays. For the automated assays, the entire procedure -from samples dilution to final readouts-was performed by CHORUS TRIO instrument (DIESSE, Italy). Residual human serum samples from clinical laboratory investigations and samples resulting from the addition of specific drugs (IFX or ADL) or anti-drug antibodies (anti-IFX or anti-ADL) were used for the characterization and validation of the tests. Results: CHORUS Promonitor kits showed an excellent agreement (Cohen's coefficient = 1) with the Promonitor kits and were linear within predefined ranges. All assays were accurate and repeatable, as an acceptable variability were observed within runs, between runs, lots, and instruments. No difference in detecting the reference drug or biosimilars emerged. Conclusion: During preclinical development, these kits resulted as sensitive, specific, accurate, and able to quantify either the reference drug or the corresponding biosimilars. All these features support their use in clinical practice for therapeutic drug monitoring of patients with inflammatory diseases under treatment with IFX or ADL

Decomposition of Arachis pintoi and Hyparrhenia rufa litters in monoculture and intercropped systems under lowland soil.

ABSTRACT - Tropical grasslands under lowland soils are generally underutilized and the litter of forage legumes may be used to recover these degraded pastures. The objective of this work was to study the dynamics of litter decomposition of Arachis pintoi (pinto peanut), Hyparrhenia rufa (thatching grass) and a mixture of both species in a lowland soil. These treatments were analyzed in three areas: grass monoculture, legume monoculture and legume intercropped with the grass during the dry and wet seasons. Litter bags containing the legume, grass or a mixture of both species were incubated to estimate the decomposition rate and microorganism colonization. Decomposition constants (K) and litter half-lives (T1/2) were estimated by an exponential model whereas number of microorganisms in specific media were determined by plate dilution. The decomposition rate, release of nutrients and microorganisms number, especially bacteria, increased when pinto peanut was added to thatching grass, influenced by favorable lignin/N and C/N ratios in legume litter. When pinto peanut litter was incubated in the grass plots, 50% N and P was released within about 135 days in the dry season and in the wet season, the equivalent release occurred within 20 days. These results indicate that A. pintoi has a great potential for nutrient recycling via litter and can be used to recover degraded areas. RESUMO - Pastagens tropicais sobre solos de várzea são geralmente subutilizadas. A serrapilheira de leguminosas forrageiras pode ser usada para a recuperação destas pastagens. O objetivo deste trabalho foi estudar a dinâmica de decomposição de Arachis pintoi (arachis), Hyparrhenia rufa (capim jaraguá) e da mistura destas espécies, em solo de várzea. Estes tratamentos foram analisados em três áreas: monocultivo da gramínea; monocultivo da leguminosa e no consórcio entre as espécies durante as estações seca e chuvosa. Sacos de decomposição contendo a serrapilheira da leguminosa ou da mistura das espécies foram incubados para estimar a taxa de decomposição e colonização microbiana. A taxa de decomposição e o tempo de meia-vida (T1/2) foram estimados por um modelo exponencial, e o número de microrganismos foi determinado por meio de diluição em placas em meios específicos. A liberação de nutrientes, a taxa de decomposição, e o número de microrganismos, especialmente bactérias, aumentaram quando arachis foi adicionado ao capim jaraguá, com influência da relação favorável de lignina/N e C/N do resto desta cultura. Quando arachis foi incubado em parcelas de monocultivo da gramínea, 50% do total de N e P foi liberado em 135 dias na estação seca e em 20 dias na estação chuvosa. Estes resultados indicam que A. pintoi tem um grande potencial para ciclagem de nutrientes e pode ser uma estratégia na recuperação de áreas degradadas

Effectiveness of synthetic calcite doped with Fe-EDDHSA as a slow-release Fe source: In-vitro experiment on kiwifruit (Actinidia chinensis var. deliciosa) plants

Doped calcite (Fe-EDDHSA/CaCO3) was experimentally produced. The hypothesis of the present experiment is that, when roots get in contact with Fe-EDDHSA/CaCO3, the extrusion of H+ decreases the pH and dissolves calcite with subsequent release of Fe that becomes available for roots. The aim of the experiment was to determine whether doped calcite might represent a slow-release Fe source for in-vitro grown kiwifruit plantlets. The root elongation media used in the experiment had pH 8.0 and differed from each other for Fe supply as follow: Control medium that contained complete Murashige and Skoog salt mixture, including FeSO4 and Na(2)EDTA; calcite medium enriched with Fe-EDDHSA/CaCO3 as the only Fe source; -Fe medium without Fe. The absence of FeSO4 in the medium caused a reduction of plantlet growth. The final pH was higher with calcite medium than in control and -Fe. The addition of Fe-EDDHSA/CaCO3 increased Fe shoot concentration when compared with the -Fe medium. The data of the present experiment show the potential Fe slow release ability of Fe-EDDHSA/CaCO3; however, further investigation on Fe containing fertilizers should be conducted on potted plants to validate our result

Unraveling the mechanism of the one-pot synthesis of exchange coupled Co-based nano-heterostructures with a high energy product

The development of reproducible protocols to synthesize hard/soft nano-heterostructures (NHSs) with tailored magnetic properties is a crucial step to define their potential application in a variety of technological areas. Thermal decomposition has proved to be an effective tool to prepare such systems, but it has been scarcely used so far for the synthesis of Co-based metal/ferrite NHSs, despite their intriguing physical properties. We found a new approach to prepare this kind of nanomaterial based on a simple one-pot thermal decomposition reaction of metal-oleate precursors in the high boiling solvent docosane. The obtained NHSs are characterized by the coexistence of Co metal and Co doped magnetite and are highly stable in an air atmosphere, thanks to the passivation of the metal with a very thin oxide layer. The investigation of the influence of the metal precursor composition (a mixed iron-cobalt oleate), of the ligands (oleic acid and sodium oleate) and of the reaction time on the chemical and structural characteristics of the final product, allowed us to rationalize the reaction pathway and to determine the role of each parameter. In particular, the use of sodium oleate is crucial to obtain a metal phase in the NHSs. In such a way, the one-pot approach proposed here allows the fine control of the synthesis, leading to the formation of stable, high performant, metal/ferrite NHSs with tailored magnetic properties. For instance, the room temperature maximum energy product was increased up to 19 kJ m-3 by tuning the Co content in the metal precursor

Does organic farming increase raspberry quality, aroma and beneficial bacterial biodiversity?

Plant-associated microbes can shape plant phenotype, performance, and productivity. Cultivation methods can influence the plant microbiome structure and differences observed in the nutritional quality of differently grown fruits might be due to variations in the microbiome taxonomic and functional composition. Here, the influence of organic and integrated pest management (IPM) cultivation on quality, aroma and microbiome of raspberry (Rubus idaeus L.) fruits was evaluated. Differences in the fruit microbiome of organic and IPM raspberry were examined by next-generation sequencing and bacterial isolates characterization to highlight the potential contribution of the resident-microflora to fruit characteristics and aroma. The cultivation method strongly influenced fruit nutraceutical traits, aroma and epiphytic bacterial biocoenosis. Organic cultivation resulted in smaller fruits with a higher anthocyanidins content and lower titratable acidity content in comparison to IPM berries. Management practices also influenced the amounts of acids, ketones, aldehydes and monoterpenes, emitted by fruits. Our results suggest that the effects on fruit quality could be related to differences in the population of Gluconobacter, Sphingomonas, Rosenbergiella, Brevibacillus and Methylobacterium on fruit. Finally, changes in fruit aroma can be partly explained by volatile organic compounds (VOCs) emitted by key bacterial genera characterizing organic and IPM raspberry fruit