6 research outputs found
A species-specific polymerase chain reaction assay for rapid and sensitive detection of Sclerotium rolfsii
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No full textNot AvailableThe aim of the present study was to characterize ten selected isolates of Trichoderma with differential
inhibitory potential over the collar rot pathogen of Amorphophallus, Sclerotium rolfsii. Use of a polyphasic
approach which combined the morphological, molecular and biochemical attributes revealed inter specific
and intra specific diversity among different isolates. Strains with the best in vitro antagonistic capacity
were Trichoderma harzianum (Tr9) and Trichoderma asperellum (Tr10). In general, relationship was
observed between the anatgonistic capacity of the Trichoderma spp. and their in vitro biocontrol performance,
cell wall degrading enzyme production, protein profiles and the RAPD and AFLP finger print. The
pot experiment revealed a comparative success of Tr9 in controlling collar rot incidence in
Amorphophallus over Tr10. This is the first report of intensive characterization of Trichoderma strains with
the integration of the above data sets against S. rolfsii, in an attempt to explore their biocontrol potential
in managing collar rot disease.Not Availabl
Rapid and sensitive detection of Phytophthora colocasiae associated with leaf blight of taro by species-specific polymerase chain reaction assay
No full textCharacterization of Trichoderma isolates against Sclerotium rolfsii, the collar rot pathogen of Amorphophallus – A polyphasic approach
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