Development of a nursery good agricultural practices framework and protocol for the South African citrus nursery industry

No abstract available.Dissertation (MInstAgrar)--University of Pretoria, 2011.Microbiology and Plant Pathologyunrestricte

Generating functions and the enumeration of lattice paths

A thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in ful lment of the requirements for the degree of Master of Science. Johannesburg, 2013.Our main focus in this research is to compute formulae for the generating function of lattice paths. We will only concentrate on two types of lattice paths, Dyck paths and Motzkin paths. We investigate di erent ways to enumerate these paths according to various parameters. We start o by studying the relationship between the Catalan numbers Cn, Fine numbers Fn and the Narayana numbers vn;k together with their corresponding generating functions. It is here where we see how the the Lagrange Inversion Formula is applied to complex generating functions to simplify computations. We then study the enumeration of Dyck paths according to the semilength and parameters such as, number of peaks, height of rst peak, number of return steps, e.t.c. We also show how some of these Dyck paths are related. We then make use of Krattenhaler's bijection between 123-avoiding permutations of length n, denoted by Sn(123), and Dyck paths of semilength n. Using this bijective relationship over Sn(123) with k descents and Dyck paths of semilength n with sum of valleys and triple falls equal to k, we get recurrence relationships between ordinary Dyck paths of semilength n and primitive Dyck paths of the same length. From these relationships, we get the generating function for Dyck paths according to semilength, number of valleys and number of triple falls. We nd di erent forms of the generating function for Motzkin paths according to length and number of plateaus with one horizontal step, then extend the discussion to the case where we have more than one horizontal step. We also study Motzkin paths where the horizontal steps have di erent colours, called the k-coloured Motzkin paths and then the k-coloured Motzkin paths which don't have any of their horizontal steps lying on the x-axis, called the k-coloured c-Motzkin paths. We nd that these two types of paths have a special relationship which can be seen from their generating functions. We use this relationship to simplify our enumeration problems

Isolation and characterisation of a xylanase producing isolate from straw-based compost

>Magister Scientiae - MScLignocellulosic biomass, a waste component of the agricultural industry, is a promising source for use in bioethanol production. Due to a complex structure, the synergistic action of lignocellulosic enzymes is required to achieve complete digestion to fermentable sugars. This study aimed to isolate, identify and characterise novel lignocellulase producing bacteria from thermophilic straw-based compost (71°C). Colonies with different morphological characteristics were isolated and screened for lignocellulosic activity. A facultative aerobic isolate RZ1 showed xylanase, cellulase and lipase/esterase activity. In addition to these activities, it was also able to produce proteases, catalases, amylases and gelatinases. RZ1 cells were motile, rod-shaped, Gram positive and endospore forming. The growth temperature of isolate RZ1 ranged from 25-55°C with optimal growth at 37°C. The 16S rRNA gene sequence was 99% identical to that of Bacillus subtilis strain MSB10. Based on the biochemical and physiological characteristics and 16S rRNA gene sequence, isolate RZ1 is considered a member of the species B. subtilis. A small insert genomic library with an average insert size of 5 kb was constructed and screened for lignocellulosic activity. An E.coli plasmid clone harbouring a 4.9 kb gDNA fragment tested positive for xylanase activity. The xyl R gene was identified with the aid of transposon mutagenesis and the deduced amino acid sequence showed 99% similarity to an endo-1-4-β-xylanase from B. pumilus. High levels of xylanases were produced when isolate RZ1 was cultured (37°C) with beechwood xylan as a carbon source. On the other hand, the production of xylanases was inhibited in the presence of xylose. Marked xylanase activity was measured in the presence of sugarcane bagasse, a natural lignocellulosic substrate. While active at 50°C, higher xylanase activity was detected at 37°C. Isolate RZ1 also produced accessory enzymes such as β-xylosidases and α-L-arabinofuranosidases, able to hydrolyse hemicellulose

Pesticide residues and estrogenic activity in fruit and vegetables sampled from major fresh produce markets in South Africa

Food is likely to be one of the major pathways through which people are exposed to endocrinedisrupting chemicals. With the exception of residual effects, there are concerns that a number of naturally occurring and synthetic chemicals exert adverse effects upon endocrine systems in wildlife and humans. The current study reports selected pesticide concentrations and the total estrogenic activity of fruit and vegetables using the recombinant yeast oestrogen screen (YES) and T47D-KBluc reporter gene assays. A total of 53 food samples (27 fruit and 26 vegetables) from Johannesburg and Tshwane fresh produce markets (in South Africa) were analysed. Of these, 17 contained one to three different pesticide residues with concentrations ranging between 0.01 and 0.68 mg kg–1, whereas in the rest of the samples no residues were detected. All pesticides detected except in one sample were below the maximum residue level (MRL), but others were unauthorised for use in specified fruit and vegetables. Estrogenic activity was detected in 26.4% (14 samples) of the samples tested, and the estradiol equivalents ranged from 0.007 to 2 pg g–1. Although the estrogenic activity was low, it may contribute to adverse health effects. Continuous monitoring for pesticides in fruit and vegetables is important in view of the unauthorised pesticides detected in produce from South Africa and the endocrine-disrupting chemical activity found.South African Table Grape Industry, the University of Pretoria, Institute of Food Nutrition and Well-Being, Department of Science and Technology and the National Research Foundation administered Centre of Excellence in Food Security (Project No 140701).http://www.tandfonline.com/loi/tfac202017-07-30hb201

Duet for zwipoṱoliyo

Indigenous musi

Tshireshi

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Vhanna vha a jelosa dzhelosa

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John (Dzhon) Ṅwamahebe

Indigenous musi