Genetic diversity studies in common bean (Phaseolus vulgaris L.) using molecular markers

Molecular characterization of thirteen common bean genotypes was done with random amplified polymorphic DNA (RAPD) markers. Initially, 15 primers were screened out of which only seven were selected which generated a total of 65 amplification products out of which 63 bands (96.62%) were polymorphic indicating fair amount of polymorphism. The genotypes shared 43% genetic similarity among themselves. Cluster analysis delineated the genotypes into three groups with seven, five and one genotype in cluster-I, II and III, respectively. The maximum similarity index (82.35) based dice similarity coefficient was obtained between SKUA-R-21 and SKUA-R-19, while it was minimum (27.72) between genotypes PBG-29 and SKUA-R-01.Key words: Genetic divergence, common bean, random amplified polymorphic DNA (RAPD)

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Toxic action of aqueous wheat straw extract on horse e purslane Ação tóxica do extrato aquoso da palha de trigo sobre beldroega cavalo

The toxic action of aqueous wheat (Triticum aestivum) straw extracts was investigated on germination, early seedling growth, some biochemical attributes and the antioxidant enzymes of horse purslane (Trianthemaportulacastrum). Aqueous extracts of wheat straw were prepared by soaking the wheat straw in distilled water in 1:10 w/v ratio and diluted to obtain the concentrations of 0, 25, 50, 75 and 100%. These were used as pre and post emergence in laboratory and screen house trials. Wheat aqueous extracts exhibited phytotoxicity to horse purslane by inhibiting and delaying its germination and suppressing seedling growth. Wheat phytotoxins in its aqueous extracts suppressed the chlorophyll content and soluble protein, and enhanced soluble phenolics and the activity of antioxidant enzymes as catalase, peroxidase and superoxide dismutase in the seedlings of horse purslane compared with the control. Such inhibitory activity is believed to originate from exposure to wheat phytotoxins that are present in its aqueous straw extract. The suppressive effects of wheat straw need to be investigated further under field conditions.A ação tóxica de extratos aquosos da palha de trigo (Triticum aestivum) foi investigada na germinação, no crescimento inicial da plántula, em alguns atributos bioquímicos e nas enzimas antioxidantes de beldroegas-cavalo (Thianthema portulacastrum). Extratos aquosos de palha de trigo foram preparados pela imersão deste em água destilada na proporção de 01:10 m/v e diluidos para obtenção de cinco concentrações (0, 25, 50, 75 e 100%). Estes foram usados como pré e pós-emergência em ensaios de laboratório e casa de vegetação. Os extratos aquosos de trigo exibiram toxicidade às beldroegas-cavalo, inibindo e retardando a sua germinação e suprimindo o crescimento das plántulas. Toxinas no extrato aquoso de trigo suprimiram o teor de clorofila e proteína solúvel, aumentaram os fenóis solúveis e a atividade de enzimas antioxidantes como catalase, peroxidase e superóxido dismutase em plántulas de beldroega-cavalo comparado ao controle. Acreditase que essa atividade inibitória origina-se da exposição das beldroegas-cavalo às toxinas presentes no extrato aquoso de palha de trigo. Efeitos supressivos da palha de trigo precisam ser investigados em condições de campo

Effect of crop residues applied isolated or in combination on the germination and seedling growth of horse purslane (Trianthema portulacastrum)

Soil incorporation of crop residues can lead to weed suppression by posing allelopathic and physical effects. Allelopathic potential of the crops sorghum, sunflower, brassica applied as sole or in combination for horse purslane (Trianthema portulacastrum) suppression was evaluated in a pot investigation. Chopped crop residues alone and in combination were incorporated at 6 g kg-1 soil (12 t ha-1), and a weedy check was maintained. Germination traits time to start germination; time to 50% emergence, mean emergence time, emergence index and final germination percentage were negatively influenced by residue incorporation. Crop residues also exerted a pronounced negative influence on the shoot and root length of horse purslane. Significant suppression in leaf and root score and leaf area per plant was also observed. A combination of sorghum and sunflower residues accounted for maximum (71%) seedling mortality. Soil incorporation of allelopathic crop residues can be employed for horse purslane management

Development of Electrode-Supported Proton Conducting Solid Oxide Cells and their Evaluation as Electrochemical Hydrogen Pumps

Protonic ceramic solid oxide cells (P-SOCs) have gained widespread attention due to their potential for operation in the temperature range of 300–500 °C, which is not only beneficial in terms of material stability but also offers unique possibilities from a thermodynamic point of view to realize a series of reactions. For instance, they are ideal for the production of synthetic fuels by hydrogenation of carbon dioxide and nitrogen, upgradation of hydrocarbons, or dehydrogenation reactions. However, the development of P-SOC is quite challenging because it requires a multifront optimization in terms of material synthesis and fabrication procedures. Herein, we report in detail a method to overcome various fabrication challenges for the development of efficient and robust electrode-supported P-SOCs (Ni-BCZY/BCZY/Ni-BCZY) based on a BaCe0.2Zr0.7Y0.1O3−δ (BCZY271) electrolyte. We examined the effect of pore formers on the porosity of the Ni-BCZY support electrode, various electrolyte deposition techniques (spray, spin, and vacuum-assisted), and thermal treatments for developing robust and flat half-cells. Half-cells containing a thin (10–12 μm) pinhole-free electrolyte layer were completed by a screen-printed Ni-BCZY electrode and evaluated as an electrochemical hydrogen pump to access the functionality. The P-SOCs are found to show a current density ranging from 150 to 525 mA cm–2 at 1 V over an operating temperature range of 350–450 °C. The faradaic efficiency of the P-SOCs as well as their stability were also evaluated

Development of Electrode-Supported Proton Conducting Solid Oxide Cells and their Evaluation as Electrochemical Hydrogen Pumps

Protonic ceramic solid oxide cells (P-SOCs) have gained widespread attention due to their potential for operation in the temperature range of 300–500 °C, which is not only beneficial in terms of material stability but also offers unique possibilities from a thermodynamic point of view to realize a series of reactions. For instance, they are ideal for the production of synthetic fuels by hydrogenation of carbon dioxide and nitrogen, upgradation of hydrocarbons, or dehydrogenation reactions. However, the development of P-SOC is quite challenging because it requires a multifront optimization in terms of material synthesis and fabrication procedures. Herein, we report in detail a method to overcome various fabrication challenges for the development of efficient and robust electrode-supported P-SOCs (Ni-BCZY/BCZY/Ni-BCZY) based on a BaCe0.2Zr0.7Y0.1O3−δ (BCZY271) electrolyte. We examined the effect of pore formers on the porosity of the Ni-BCZY support electrode, various electrolyte deposition techniques (spray, spin, and vacuum-assisted), and thermal treatments for developing robust and flat half-cells. Half-cells containing a thin (10–12 μm) pinhole-free electrolyte layer were completed by a screen-printed Ni-BCZY electrode and evaluated as an electrochemical hydrogen pump to access the functionality. The P-SOCs are found to show a current density ranging from 150 to 525 mA cm–2 at 1 V over an operating temperature range of 350–450 °C. The faradaic efficiency of the P-SOCs as well as their stability were also evaluated

Development of Electrode-Supported Proton Conducting Solid Oxide Cells and their Evaluation as Electrochemical Hydrogen Pumps

Protonic ceramic solid oxide cells (P-SOCs) have gained widespread attention due to their potential for operation in the temperature range of 300–500 °C, which is not only beneficial in terms of material stability but also offers unique possibilities from a thermodynamic point of view to realize a series of reactions. For instance, they are ideal for the production of synthetic fuels by hydrogenation of carbon dioxide and nitrogen, upgradation of hydrocarbons, or dehydrogenation reactions. However, the development of P-SOC is quite challenging because it requires a multifront optimization in terms of material synthesis and fabrication procedures. Herein, we report in detail a method to overcome various fabrication challenges for the development of efficient and robust electrode-supported P-SOCs (Ni-BCZY/BCZY/Ni-BCZY) based on a BaCe0.2Zr0.7Y0.1O3−δ (BCZY271) electrolyte. We examined the effect of pore formers on the porosity of the Ni-BCZY support electrode, various electrolyte deposition techniques (spray, spin, and vacuum-assisted), and thermal treatments for developing robust and flat half-cells. Half-cells containing a thin (10–12 μm) pinhole-free electrolyte layer were completed by a screen-printed Ni-BCZY electrode and evaluated as an electrochemical hydrogen pump to access the functionality. The P-SOCs are found to show a current density ranging from 150 to 525 mA cm–2 at 1 V over an operating temperature range of 350–450 °C. The faradaic efficiency of the P-SOCs as well as their stability were also evaluated.</p

Mechanisms underlying vasorelaxation induced in the porcine coronary arteries by Thymus linearis , Benth

International audienc