Kajian Kualitas Fisik. Kimia dan Sensoris Susu Pasteurisasi pada Pasteurizer Berbeda.

The research was conducted to know the chemical, physical and sensorical Quality of the pasteurized milk using two different tools in the refrigerator storage.The milktakenfrom cows which milched in the morningthenpasteurizedusing twotoolsnamelyPADIRApateurizerandSafgardHomePasteurizer. Variablesobservedincludealcoholtest, density test, lipid content, acid degree value of lipid globular protein content, and sensorical test includen sweetned, acidity and rancidity degree. Density test, lipid content,acid degreevalue of lipid, globular protein congtent were analiyzed with split-splot design, but alcoholtest, sweetnedand aciditywere analyzedwithdescriptiveanalysisand rancidity(0.33% and 0.86%) were analyzedwithchi-square with kruskal-Wallis method. The results showed pasteurization on PADIRA Pasteurizerbetter than Safgard Homepasteurized

The Occurrence of CSN1S1 E Allele on Saanen Goat Population at BBPTU-HPT Baturraden

The CSN1S1 gene is expressed as the milk alpha S1 casein which is valuable for cheese making. Allele E is a mutation of this gene, which is correlated with low quantity of expressed protein. This study aimed to identify the occurrence of CSN1S1 E allele on the Saanen population at BBPTU-HPT Baturraden. Blood samples of 45 does were drawn, followed by DNA isolation using the column spin method. The identification of E allele was carried out by Allele Specific Polymerase Chain Reactions (AS-PCR) with forward primer 5-TCAGGAGCAGTGGGTATGTG-3 and reverse 5-CCTCCCAATGGAATAATGACA-3. Allele E was found as heterozygote genotype with non-E allele in 28 out of 45 samples. This means that F allele frequency was 0.31 and 0.6 for non-E allele. There was no significant difference of milk protein in both groups. In summary, E allele was found in the Saanen goat population in BBPTU HPT Baturraden with no effect on milk protein production. Keywords: Saanen, CSN1S1, milk, goats, protein

A novel reversed phase high performance liquid chromatography method to accurately determine low concentrations of curcumin in rat plasma

Due to its lipophilic nature, curcumin levels in plasma are very low after oral administration, and therefore hard to detect. A number of chromatographic methods, including LC/MS have been developed. Although the LC/MS method is sensitive, the matrix effect can be difficult to handle. Furthermore, LC/MS equipment is relatively expensive compared to the conventional RP-HPLC. Therefore, the aim of this study was to develop a sensitive and reliable method for the determination of curcumin concentration in plasma using an RP-HPLC. Curcuma longa extract was used which contains three curcuminoids. The method started by selection of mobile phase to optimally separate the curcuminoids. The best mobile phase composition was used to analyze the plasma samples. Rat plasma was spiked with curcumin and processed for protein precipitation followed by liquid-liquid extraction of curcuminoids and an internal standard, emodin. Chromatogaphic separation of curcuminoids and emodin was achieved using a Knauer C-18 column (250 x 4.6 mm; particle size: 5µm) and a gradient program of mobile phase of three solvents, methanol -acetonitrile-1% acetic acid. A gradient elution was applied with increasing the ratio of the volume percentages of acetonitrile to acetic acid from 50/45 to 53/42 during 5 minute and thereafter elution was isocratic for 15 minute. The methanol concentration was kept constant at 5 vol-% during the whole run. The method was validated according to the FDA guidelines.The method was selective, with an excellent resolution (Rs value > 2.5). The peak shape of both curcumin and emodin were symmetric with a tailing factor of 0.9-1.1. The method linearity (correlation coefficient of 0.999) was demonstrated at 6 to 200 ng/mL. The intra and inter-day precision was 5.90-8.50% and 5.37-11.26%, respectively; the intra- and inter-day accuracy was 92.47-103.61% and 96.17-105.70%, respectively. In conclusion, the RP-HPLC method meets the validation requirements as described in the FDA guidelines and is applicable to accurately quantify curcumin concentrations as low as 6 ng/mL in rat plasma samples

A novel reversed phase high performance liquid chromatography method to accurately determine low concentrations of curcumin in rat plasma

Due to its lipophilic nature, curcumin levels in plasma are very low after oral administration, and therefore hard to detect. A number of chromatographic methods, including LC/MS have been developed. Although the LC/MS method is sensitive, the matrix effect can be difficult to handle. Furthermore, LC/MS equipment is relatively expensive compared to the conventional RP-HPLC. Therefore, the aim of this study was to develop a sensitive and reliable method for the determination of curcumin concentration in plasma using an RP-HPLC. Curcuma longa extract was used which contains three curcuminoids. The method started by selection of mobile phase to optimally separate the curcuminoids. The best mobile phase composition was used to analyze the plasma samples. Rat plasma was spiked with curcumin and processed for protein precipitation followed by liquid-liquid extraction of curcuminoids and an internal standard, emodin. Chromatogaphic separation of curcuminoids and emodin was achieved using a Knauer C-18 column (250 x 4.6 mm; particle size: 5µm) and a gradient program of mobile phase of three solvents, methanol -acetonitrile-1% acetic acid. A gradient elution was applied with increasing the ratio of the volume percentages of acetonitrile to acetic acid from 50/45 to 53/42 during 5 minute and thereafter elution was isocratic for 15 minute. The methanol concentration was kept constant at 5 vol-% during the whole run. The method was validated according to the FDA guidelines.The method was selective, with an excellent resolution (Rs value > 2.5). The peak shape of both curcumin and emodin were symmetric with a tailing factor of 0.9-1.1. The method linearity (correlation coefficient of 0.999) was demonstrated at 6 to 200 ng/mL. The intra and inter-day precision was 5.90-8.50% and 5.37-11.26%, respectively; the intra- and inter-day accuracy was 92.47-103.61% and 96.17-105.70%, respectively. In conclusion, the RP-HPLC method meets the validation requirements as described in the FDA guidelines and is applicable to accurately quantify curcumin concentrations as low as 6 ng/mL in rat plasma samples

Model Pemberdayaan Masyarakat Bidang Kesehatan, Studi Program Desa Siaga

Since 2006, the Health Department had launched the Village Preparedness program policy. But, this policy apparently not capable of empowering the community in identifying and solving the health problem at community (village) level. The objective of research is to formulate the community empowerment model in health in the Village Preparedness program. The targets of research were the Village Health Forum and Village Health Post in 30 Village Preparedness in Karanganyar Regency, Central Java. The method involving survey and case study. The case study showed factors related to community capability of identifying health problem and community capability of solving the health problems. The community empowerment model in health including the capabilities of identifying and of solving the health problems. The community internal and external factors at members of community level, community institution, community leadership, and information access played important role in community empowerment in health

Excitonic Transitions and Off-resonant Optical Limiting in CdS Quantum Dots Stabilized in a Synthetic Glue Matrix

Stable films containing CdS quantum dots of mean size 3.4 nm embedded in a solid host matrix are prepared using a room temperature chemical route of synthesis. CdS/synthetic glue nanocomposites are characterized using high resolution transmission electron microscopy, infrared spectroscopy, differential scanning calorimetry and thermogravimetric analysis. Significant blue shift from the bulk absorption edge is observed in optical absorption as well as photoacoustic spectra indicating strong quantum confinement. The exciton transitions are better resolved in photoacoustic spectroscopy compared to optical absorption spectroscopy. We assign the first four bands observed in photoacoustic spectroscopy to 1se–1sh, 1pe–1ph, 1de–1dhand 2pe–2phtransitions using a non interacting particle model. Nonlinear absorption studies are done using z-scan technique with nanosecond pulses in the off resonant regime. The origin of optical limiting is predominantly two photon absorption mechanism