Maintenance and Modelling Strategies of Biomedical Equipment’s in Hospitals: A Review

This article analyzes recent developments in the derivation of bio-medical equipment’s maintenance management strategies, and proposes chary points to consider and affeer to perform during their operation. Medical technology is by now an integral part of health care according to consisting general accepted standards. Purchase and operation thereby represent an important economic position and both are subject of everyday optimization attempts. For this purpose by now exists a huge number of tools which conduce more likely to a complexness of the problem by a comprehensive implementation. In the future, we envision that new paradigms will be structured to overcome the present strategies, and in the longer term, provide an alternative source for maintenance and management of medical devices at hospitals. Keywords: Biomedical equipment’s, Hospitals, Maintenance, Medical Devices, Medical Technolog

Studies of the silkworm enzyme activity and their correlations with economic variables

The experiment was conducted to analyse the larval performance and economic traits of bivoltine silkworm breeds of silkworm (SK-1, SK-6, SK-22, SK-28, SK-33, CSR4, CSR2, NB4D2, DUN6 and APS4) during spring season. The haemolymph total protein, succinate and gultamate dehydrogenase activities were estimated and their correlation with economic traits were also worked out. The results of the study confirmed that among ten bivoltine silkworm breeds, highest haemolymph SDH activity of 3.47 µmoles/ml/mgprotein/min was recorded in the silkworm breed SK1 and least SDH activity of 1.58 µmoles/ml/mg protein/min was recorded in the breed APS4. The highest peak of succinate dehydrogenase activity of 2.65 µmoles/ml/mg protein/min was observed on 7th day of the 5th instar and lowest peak of succinate dehydrogenase activity of 2.39 µmoles/ml/mg protein/min was observed on 4th day of the 5th instar. GDH activity of 0.46 µmoles/ml/mg protein/min was recorded highest in the silkworm breed SK1 and lowest of 0.15 µmoles/ml/mg protein/min was recorded in silkworm breed APS4. The highest peak of haemolymph GDH of 0.36 µmoles/ml/mg protein/min was recorded on 7th day of 5th instar and lowest peak of 0.26 µmoles/ml/mg protein/min was recorded on 4th day of 5th instar. The correlational studies revealed that haemolymph total protein, SDH and GDH were found to be positively corelated with yield by weight and number (cocoon), weight of mature larvae, shell weight ,cocoon weight, shell ratio percent, silk productivity, rate of pupation, fecundity, raw silk percentage  and length of filament. Thus, the study revealed that silkworm breeds like SK1, SK6, SK22 and SK28 as productive breeds and hence may be used for future breeding programmes for evolution of new robust silkworm breed

Biomolecular Engineering of <i>Escherichia coli </i>Organo-mercurial Lyase Gene and its Expression

117-120Studies were carried out to characterize merB gene from five wild type strains of broad-spectrum Escherichia coli, collected from five geographically distinct regions of India. Each strain produced 23kb plasmid from which functional merB gene (0.64kb) was PCR amplified. The merB gene from isolate G18, which tolerated highest concentration of organic form (PMA) of mercury was cloned in high expression vector pQE30 and pGEMT-Easy vector. The transformants obtained demonstrated varied results in their appropriate hosts. The transformants (IAxpress) carrying merB gene cloned in pQE30 and negative control having pQE30 without merB insert did not grow on agar plates amended with 1μg/ml PMA. Due to the hyperexpression of merB in pQE30 most of the protein was found in nonfunctional inclusion bodies and did not show any resistance as sensitive strain (Devoid of merB gene) against PMA. On the other hand transformants of merB cloned pGEMT vector tolerated up to 5μg/ml of PMA, which indicates that low expression of merB in this vector produces a functional product and thus tolerates five times more PMA than sensitive strain. The results demonstrate that this gene can be better exploited for bioremediation of toxic form of mercury in polluted water bodies

Chemo profiling and antioxidant activity of different genotypes of Brassica oleracea var acephala grown across Kashmir in different environments

Green leafy vegetables are gaining significant interest due to their nutritional value and disease-preventing potential. The Kale (Brassica oleracea&nbsp;var.&nbsp;acephala) is known as the queen of vegetables in some parts of the world due to its higher nutritional value. The current comparative study evaluated total phenols, total flavonoids, glucosinolates, and antioxidant activity in thirty different genotypes of kale grown across the Kashmir valley in different environments. The leaves were collected at the edible stage, and the extracts were made using three different solvent systems (80% methanol, 80% ethanol, and water). The majority of the genotypes of kale indicated a difference in the content of bioactive compounds. Based on the accumulation of nutraceutical important phenols, flavonoids, alkaloids, and sterols, the 30 genotypes of kale indicated a difference in bioactive compounds' content. The aqueous extract of K-29 demonstrated the phenolic content (71.68mg/gallic acid/g DW) and 60.98μMol/g DW glucosinolate content. Moreover, the methanolic extract of the K-29 genotype also indicated the higher phenolic content (85.56 mg/gallic acid/g DW), flavonoid content (16.64 mg/quercetin/g DW) as well as antioxidant activity (92.56 μMol FRAP/g DW). The higher antioxidant potential of K-29 is corroborated by its higher metabolic content of phenols and flavonoids.&nbsp

Nutritional Profiling, Phytochemical Composition and Antidiabetic Potential of <i>Taraxacum officinale</i>, an Underutilized Herb

Taraxacum officinale (T. officinale), a wild vegetable with a number of health claims, has been mostly ignored and unexplored. The study aims to compare the nutritional, phytochemical as well as antidiabetic potential of fresh as well as shade-dried leaves of T. officinale, in order to recommend its best form as a dietary antidiabetic product. The results revealed that as compared to fresh leaves, the shade-dried leaves, in addition to possessing higher levels of carbohydrates, crude protein, crude fat, crude fiber, etc., also contain appreciable amounts of total phenols (5833.12 ± 4.222 mg/100), total flavonoids (188.84 ± 0.019 mg/100 g), ascorbic acid (34.70 ± 0.026 mg/100 g), β-carotene (3.88 ± 1.473 mg/100 g) and total chlorophyll (239.51 ± 0.015 mg/100 g) antioxidants. The study revealed the presence of medicinally important antidiabetic flavonoid quercetin present in T. officinale leaves. Among the three solvent systems used, the aqueous extract of shade-dried T. officinale leaves comparatively demonstrated potent antidiabetic activity under in vitro conditions in a dose-dependent manner via targeting α-amylase and α-glucosidase, the two potent enzymes of carbohydrate metabolism. Therefore, in addition to being a nutritious herb, the shade-dried leaves of T. officinale have great potential to suppress post-prandial glucose rise and can be better exploited through clinical trials to be used as a dietary intervention for better management of diabetes

Fe and Zn stress induced gene expression analysis unraveled mechanisms of mineral homeostasis in common bean (Phaseolus vulgaris L.)

Iron (Fe) and zinc (Zn) stress significantly affects fundamental metabolic and physiological processes in plants that results in reduction of plant growth and development. In the present study, common bean variety; Shalimar French Bean-1 (SFB-1) was used as an experimental material. Four different MGRL media i.e. normal MGRL medium (Control), media without Fe (0-Fe), media without Zn (0-Zn) and media with excess Zn (300-Zn) were used for growing seeds of SFB-1 under in vitro condition for three weeks under optimum conditions. Three week old shoot and root tissues were harvested from the plants grown in these four different in vitro conditions and were, subjected to Fe and Zn estimation. Further, extraction of total RNA for differential gene expression of ten candidate genes selected based on our in silico investigation and their classification, phylogeny and expression pattern was unraveled. Expression analysis of three candidate genes (OPT3, NRAMP2 and NRAMP3) in roots revealed possible cross talk among Fe/Zn stress that was further confirmed by observing less accumulation of Fe in roots under both these conditions. However, we observed, higher accumulation of Fe in shoots under 0-Fe condition compared to control that suggests precise sensing for priority based compartmentalization and partitioning leading to higher accumulation of Fe in shoots. Furthermore, the expression analysis of IRT1, FRO1 and Ferritin 1 genes under Fe/Zn stress suggested their role in uptake/transport and signaling of Fe and Zn, whereas the expression of ZIP2, NRAMP1, HA2 and GLP1 genes were highly responsive to Zn in Phaseolus vulgaris. The identified genes highly responsive to Fe and Zn stress condition can be potential candidates for overcoming mineral stress in dicot crop plants

The dynamic condylar screw in the management of subtrochanteric fractures: does judicious use of biological fixation enhance overall results?

Subtrochanteric fractures are fraught with certain anatomic, biologic and biomechanical challenges. Evolution of implants like the Gamma nail, fixed-angle nail plates, compression hip screws and dynamic hip screws with trochanteric stabilization plates underlines a persistent quest for a better implant. We studied the dynamic condylar screw DCS as an implant on a series of 30 consecutive patients with subtrochanteric fractures. Our purpose was to assess this implant as a panacea for subtrochanteric fractures. All cases of AO type A and B were anatomically fixed, whereas type C was biologically plated. The idea was to assess the applicability and adaptability of the DCS. Fractures in 29 cases united, with one patient suffering from an implant failure. There were 17 excellent, 5 good, 5 fair and 3 poor results. The DCS is a definite advance over previous methods of treatment; when combined with the utilization of biological fixation techniques for comminuted fractures, can be relied upon to treat all types of subtrochanteric fractures