The effect of a planet on the dust distribution in a 3D protoplanetary disk

Aims: We investigate the behaviour of dust in protoplanetary disks under the action of gas drag in the presence of a planet. Our goal is twofold: to determine the spatial distribution of dust depending on grain size and planet mass, and therefore to provide a framework for interpretation of coming observations and future studies of planetesimal growth. Method: We numerically model the evolution of dust in a protoplanetary disk using a two-fluid (gas + dust) Smoothed Particle Hydrodynamics (SPH) code, which is non-self-gravitating and locally isothermal. The code follows the three dimensional distribution of dust in a protoplanetary disk as it interacts with the gas via aerodynamic drag. In this work, we present the evolution of a minimum mass solar nebula (MMSN) disk comprising 1% dust by mass in the presence of an embedded planet. We run a series of simulations which vary the grain size and planetary mass to see how they affect the resulting disk structure. Results: We find that gap formation is much more rapid and striking in the dust layer than in the gaseous disk and that a system with a given stellar, disk and planetary mass will have a completely different appearance depending on the grain size. For low mass planets in our MMSN disk, a gap can open in the dust disk while not in the gas disk. We also note that dust accumulates at the external edge of the planetary gap and speculate that the presence of a planet in the disk may enhance the formation of a second planet by facilitating the growth of planetesimals in this high density region.Comment: 13 pages, 12 figures. Accepted for publication in Astronomy & Astrophysic

The effects of tidally induced disc structure on white dwarf accretion in intermediate polars

We investigate the effects of tidally induced asymmetric disc structure on accretion onto the white dwarf in intermediate polars. Using numerical simulation, we show that it is possible for tidally induced spiral waves to propagate sufficiently far into the disc of an intermediate polar that accretion onto the central white dwarf could be modulated as a result. We suggest that accretion from the resulting asymmetric inner disc may contribute to the observed X-ray and optical periodicities in the light curves of these systems. In contrast to the stream-fed accretion model for these periodicities, the tidal picture predicts that modulation can exist even for systems with weaker magnetic fields where the magnetospheric radius is smaller than the radius of periastron of the mass transfer stream. We also predict that additional periodic components should exist in the emission from low mass ratio intermediate polars displaying superhumps.Comment: 9 pages, 5 figures, accepted for publication in MNRA

The Application of CRISPR Technology to High Content Screening in Primary Neurons

Axon growth is coordinated by multiple interacting proteins that remain incompletely characterized. High content screening (HCS), in which manipulation of candidate genes is combined with rapid image analysis of phenotypic effects, has emerged as a powerful technique to identify key regulators of axon outgrowth. Here we explore the utility of a genome editingapproach referred to as CRISPR (Clustered Regularly Interspersed Palindromic Repeats) for knockout screening in primary neurons. In the CRISPR approach a DNA-cleaving Cas enzyme is guided to genomic target sequences by user-created guide RNA (sgRNA), where it initiates a double-stranded break that ultimately results in frameshift mutation and loss of protein production. Using electroporation of plasmid DNA that co-expresses Cas9enzyme and sgRNA, we first verified the ability of CRISPR targeting to achieve protein-level knockdown in cultured postnatal cortical neurons. Targeted proteins included NeuN (RbFox3) and PTEN, a well-studied regulator of axon growth. Effective knockdown lagged at least four days behind transfection, but targeted proteins were eventually undetectable by immunohistochemistry in \u3e 80% of transfected cells. Consistent with this, anti-PTEN sgRNA produced no changes in neurite outgrowth when assessed three days post-transfection. When week-long cultures were replated, however, PTEN knockdown consistently increased neurite lengths. These CRISPR-mediated PTEN effects were achieved using multi-well transfection and automated phenotypic analysis, indicating the suitability of PTEN as a positive control for future CRISPR-based screening efforts. Combined, these data establish an example of CRISPR-mediated protein knockdown in primary cortical neurons and its compatibility with HCS workflows

Excitable Patterns in Active Nematics

We analyze a model of mutually-propelled filaments suspended in a two-dimensional solvent. The system undergoes a mean-field isotropic-nematic transition for large enough filament concentrations and the nematic order parameter is allowed to vary in space and time. We show that the interplay between non-uniform nematic order, activity and flow results in spatially modulated relaxation oscillations, similar to those seen in excitable media. In this regime the dynamics consists of nearly stationary periods separated by "bursts" of activity in which the system is elastically distorted and solvent is pumped throughout. At even higher activity the dynamics becomes chaotic.Comment: 4 pages, 4 figure

Enhancing Social Connectedness in Anxiety and Depression Through Amplification of Positivity: Preliminary Treatment Outcomes and Process of Change.

BackgroundAnxiety and depressive disorders are often characterized by perceived social disconnection, yet evidence-based treatments produce only modest improvements in this domain. The well-established link between positive affect (PA) and social connectedness suggests that directly targeting PA in treatment may be valuable.MethodA secondary analysis of a waitlist-controlled trial (N=29) was conducted to evaluate treatment response and process of change in social connectedness within a 10-session positive activity intervention protocol-Amplification of Positivity (AMP)-designed to increase PA in individuals seeking treatment for anxiety or depression (ClinicalTrials.gov Identifier: NCT02330627). Perceived social connectedness and PA/negative affect (NA) were assessed throughout treatment. Time-lagged multilevel mediation models examined the process of change in affect and connectedness throughout treatment.ResultsThe AMP group displayed significantly larger improvements in social connectedness from pre- to post-treatment compared to waitlist; improvements were maintained through 6-month follow-up. Within the AMP group, increases in PA and decreases in NA both uniquely predicted subsequent increases in connectedness throughout treatment. However, experiencing heightened NA throughout treatment attenuated the effect of changes in PA on connectedness. Improvements in connectedness predicted subsequent increases in PA, but not changes in NA.ConclusionsThese preliminary findings suggest that positive activity interventions may be valuable for enhancing social connectedness in individuals with clinically impairing anxiety or depression, possibly through both increasing positive emotions and decreasing negative emotions

The Ionization State of Sodium in Galactic Winds

Roughly 80% of Ultraluminous Infrared Galaxies (ULIRGs) show blue shifted absorption in the resonance lines of neutral sodium, indicating that cool winds are common in such objects, as shown by Rupke et al and by Martin. The neutral sodium (NaI) columns indicated by these absorption lines are ~ 10^{13}-3x10^{14}/cm^2, while the bolometric luminosity varies by a factor of only four. We show that the gas in ULIRG outflows is likely to be in photoionization equilibrium. The very small ULIRG sample of Goldader et al. demonstrates that the ratio of ultraviolet flux to far infrared flux varies by a factor $\sim100$ from object to object. While the Goldader sample does not overlap with those of Rupke et al. and Martin, we show that such a large variation in ultraviolet flux will produce a similar variation in the column of neutral sodium for a fixed mass flux and density. However, if the cold gas is in pressure equilibrium with a hot outflow with a mass loss rate similar to the star formation rate, the range of ionization state is significantly smaller. Measurements of the UV flux for objects in the Martin and Rupke et al. catalogs will definitively determine if photoionization effects are responsible for the wide variation seen in the sodium columns. If they are, a determination of the gas density and mass loss rate in the cool winds will follow, with attendant improvements in our understanding of wind driving mechanisms and of the effects of galaxies on their surroundings.Comment: 14 pages, 5 figures, submitted to Ap

A hydrogen energy carrier. Volume 2: Systems analysis

A systems analysis of hydrogen as an energy carrier in the United States indicated that it is feasible to use hydrogen in all energy use areas, except some types of transportation. These use areas are industrial, residential and commercial, and electric power generation. Saturation concept and conservation concept forecasts of future total energy demands were made. Projected costs of producing hydrogen from coal or from nuclear heat combined with thermochemical decomposition of water are in the range $1.00 to$1.50 per million Btu of hydrogen produced. Other methods are estimated to be more costly. The use of hydrogen as a fuel will require the development of large-scale transmission and storage systems. A pipeline system similar to the existing natural gas pipeline system appears practical, if design factors are included to avoid hydrogen environment embrittlement of pipeline metals. Conclusions from the examination of the safety, legal, environmental, economic, political and societal aspects of hydrogen fuel are that a hydrogen energy carrier system would be compatible with American values and the existing energy system

Structural characterization of the D290V mutation site in hnRNPA2 low-complexity-domain polymers.

Human genetic studies have given evidence of familial, disease-causing mutations in the analogous amino acid residue shared by three related RNA binding proteins causative of three neurological diseases. Alteration of aspartic acid residue 290 of hnRNPA2 to valine is believed to predispose patients to multisystem proteinopathy. Mutation of aspartic acid 262 of hnRNPA1 to either valine or asparagine has been linked to either amyotrophic lateral sclerosis or multisystem proteinopathy. Mutation of aspartic acid 378 of hnRNPDL to either asparagine or histidine has been associated with limb girdle muscular dystrophy. All three of these aspartic acid residues map to evolutionarily conserved regions of low-complexity (LC) sequence that may function in states of either intrinsic disorder or labile self-association. Here, we present a combination of solid-state NMR spectroscopy with segmental isotope labeling and electron microscopy on the LC domain of the hnRNPA2 protein. We show that, for both the wild-type protein and the aspartic acid 290-to-valine mutant, labile polymers are formed in which the LC domain associates into an in-register cross-β conformation. Aspartic acid 290 is shown to be charged at physiological pH and immobilized within the polymer core. Polymers of the aspartic acid 290-to-valine mutant are thermodynamically more stable than wild-type polymers. These observations give evidence that removal of destabilizing electrostatic interactions may be responsible for the increased propensity of the mutated LC domains to self-associate in disease-causing conformations

A hydrogen energy carrier. Volume 1: Summary

The production, technology, transportation, and implementation of hydrogen into the energy system are discussed along with the fossil fuel cycle, hydrogen fuel cycle, and the demands for energy. The cost of hydrogen production by coal gasification; electrolysis by nuclear energy, and solar energy are presented. The legal aspects of a hydrogen economy are also discussed