CREATING BETTER ROOMMATE DYADS: THE ROLD OF OPTIMISM, PESSIMISM, AND PRECEPTION WITHIN COLLEGE ROOMMATE RELATIONSHIPS

The purpose of this study is to address the relationship between optimism/pessimism, perception and roommate relationships in college students. Participants were conveniently pooled from Southeastern University and were assessed via an online survey. Literature was compiled supporting the hypotheses that the researcher created. After analysis, it was found that optimism/pessimism correlated with roommate relationships and perceived similarity of optimism and pessimism correlated with roommate relationships. These results supported some of the researcher’s hypotheses but not all of the

The Grizzly, February 21, 2019

Ursinus Turns 150 • U-Imagine Center Gives Entrepreneurial Inspiration • Exploring the Body and Mind at Ursinus • Parlee Center Highlights Brain Injuries in Sports • How Sweet the Sound: Voices in Praise Gospel Choir • God of Carnage : Dark Comedy in the Blackbox • Opinions: Blackface is Back, but it Never Really Left; Is Action-Movie Masculinity Breeding a Nation of Neesons? • The Ice in her Veins Award: Maya Kornfeld • Women\u27s Lacrosse Looking to Build on 2018 Campaign • Gymnastics Team Continues to Battle Through Tough Seasonhttps://digitalcommons.ursinus.edu/grizzlynews/1613/thumbnail.jp

The updated mouse universal genotyping array bioinformatic pipeline improves genetic QC in laboratory mice

The MiniMUGA genotyping array is a popular tool for genetic quality control of laboratory mice and genotyping samples from most experimental crosses involving laboratory strains, particularly for reduced complexity crosses. The content of the production version of the MiniMUGA array is fixed; however, there is the opportunity to improve the array's performance and the associated report's usefulness by leveraging thousands of samples genotyped since the initial description of MiniMUGA. Here, we report our efforts to update and improve marker annotation, increase the number and the reliability of the consensus genotypes for classical inbred strains and substrains, and increase the number of constructs reliably detected with MiniMUGA. In addition, we have implemented key changes in the informatics pipeline to identify and quantify the contribution of specific genetic backgrounds to the makeup of a given sample, remove arbitrary thresholds, include the Y Chromosome and mitochondrial genome in the ideogram, and improve robust detection of the presence of commercially available substrains based on diagnostic alleles. Finally, we have updated the layout of the report to simplify the interpretation and completeness of the analysis and added a section summarizing the ideogram in table format. These changes will be of general interest to the mouse research community and will be instrumental in our goal of improving the rigor and reproducibility of mouse-based biomedical research

A second planet transiting LTT 1445A and a determination of the masses of both worlds

K.H. acknowledges support from STFC grant ST/R000824/1.LTT 1445 is a hierarchical triple M-dwarf star system located at a distance of 6.86 pc. The primary star LTT 1445A (0.257 M⊙) is known to host the transiting planet LTT 1445Ab with an orbital period of 5.36 days, making it the second-closest known transiting exoplanet system, and the closest one for which the host is an M dwarf. Using Transiting Exoplanet Survey Satellite data, we present the discovery of a second planet in the LTT 1445 system, with an orbital period of 3.12 days. We combine radial-velocity measurements obtained from the five spectrographs, Echelle Spectrograph for Rocky Exoplanets and Stable Spectroscopic Observations, High Accuracy Radial Velocity Planet Searcher, High-Resolution Echelle Spectrometer, MAROON-X, and Planet Finder Spectrograph to establish that the new world also orbits LTT 1445A. We determine the mass and radius of LTT 1445Ab to be 2.87 ± 0.25 M⊕ and 1.304-0.060+0.067 R⊕, consistent with an Earth-like composition. For the newly discovered LTT 1445Ac, we measure a mass of 1.54-0.19+0.20 M⊕ and a minimum radius of 1.15 R⊕, but we cannot determine the radius directly as the signal-to-noise ratio of our light curve permits both grazing and nongrazing configurations. Using MEarth photometry and ground-based spectroscopy, we establish that star C (0.161 M⊙) is likely the source of the 1.4 day rotation period, and star B (0.215 M⊙) has a likely rotation period of 6.7 days. We estimate a probable rotation period of 85 days for LTT 1445A. Thus, this triple M-dwarf system appears to be in a special evolutionary stage where the most massive M dwarf has spun down, the intermediate mass M dwarf is in the process of spinning down, while the least massive stellar component has not yet begun to spin down.Publisher PDFPeer reviewe

Imbalanced pattern completion vs. separation in cognitive disease: network simulations of synaptic pathologies predict a personalized therapeutics strategy

<p>Abstract</p> <p>Background</p> <p>Diverse Mouse genetic models of neurodevelopmental, neuropsychiatric, and neurodegenerative causes of impaired cognition exhibit at least four convergent points of synaptic malfunction: 1) Strength of long-term potentiation (LTP), 2) Strength of long-term depression (LTD), 3) Relative inhibition levels (Inhibition), and 4) Excitatory connectivity levels (Connectivity).</p> <p>Results</p> <p>To test the hypothesis that pathological increases or decreases in these synaptic properties could underlie imbalances at the level of basic neural network function, we explored each type of malfunction in a simulation of autoassociative memory. These network simulations revealed that one impact of impairments or excesses in each of these synaptic properties is to shift the trade-off between pattern separation and pattern completion performance during memory storage and recall. Each type of synaptic pathology either pushed the network balance towards intolerable error in pattern separation or intolerable error in pattern completion. Imbalances caused by pathological impairments or excesses in LTP, LTD, inhibition, or connectivity, could all be exacerbated, or rescued, by the simultaneous modulation of any of the other three synaptic properties.</p> <p>Conclusions</p> <p>Because appropriate modulation of any of the synaptic properties could help re-balance network function, regardless of the origins of the imbalance, we propose a new strategy of personalized cognitive therapeutics guided by assay of pattern completion vs. pattern separation function. Simulated examples and testable predictions of this theorized approach to cognitive therapeutics are presented.</p

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

PRIMERJAVA ODRIVNIH ZNAČILNOSTI TREH VRST PROŽNIH PONJAV

Trampoline use has skyrocketed in recent years in a variety of recreational contexts and among athletes in sports ranging from gymnastics and diving to skiing and snowboarding. The purpose of this study was to examine the bounce characteristics elicited by athletes bouncing on three types of trampolines. Tumbl Trak, Standard, and Super Tramp trampolines were assessed by 10 experienced trampoline and acrobatic athletes (5 males, 5 females). A triaxial accelerometer (250 Hz) characterized the 10 highest controlled bounces on each trampoline and each athlete. Repeated measures ANOVAs showed statistical differences in bounce characteristics: time from bounce start to peak acceleration (p&lt;.001, ƞ2 =0.82), time from peak acceleration to bounce end (p=.030, ƞ2 =0.40), and total bounce time (p&lt;0.001, ƞ2 =0.78, jump height (p&lt;.001, ƞ2 =0.95) peak acceleration (p=.015, ƞ2 =0.37), and flight time (p&lt;.001, ƞ2 =0.97).&nbsp; Average acceleration, force, and allometrically scaled average force were not statistically different (p˃.140, ƞ2=0.20). The stiffest trampoline with the least time values, peak accelerations, and jump heights was the Tumbl Trak, followed by the Standard trampoline, and Super Tramp, respectively. This information may help practitioners and others to understand the bounce behaviors of athletes on these types of trampolines.Uporaba prožnih ponjav je v zadnjih letih močno narasla v telesni vzgoji in med športniki v športu, od orodne telovadbe in potapljanja do smučanja in deskanja na snegu. Namen te študije je bil preučiti značilnosti odrivov, ki jih izvedejo športniki, ki skačejo na treh vrstah ponjav: &nbsp;akrobatska steza, tekmovalna velika prožna ponjava in povečana velika prožna ponjava. Skoke so izvajali akrobatski (5 moških, 5 žensk). Triosni merilnik pospeška (250 Hz) je označil 10 najvišje nadzorovanih skokov na vsaki ponjavi in vsakega športnika. Ponavljajoče se analize ANOVA so pokazale značilne razlike v odrivih: čas od začetka odriva do največjega pospeševanja (p &lt;.001, =2 = 0.82), čas od pospeševanja do največjega dosega do konca (p = .030, =2 = 0.40) in skupni čas odriva (p &lt;0,001, =2 = 0,78, višina skoka (p &lt;.001, =2 = 0,95) največji pospešek (p = .015, =2 = 0,37) in čas letenja (p &lt;.001, =2 = 0,97). , sila in povprečna sila nista bili statistično različni (p˃140, =2 = 0,20). Najtrajša ponjava z najnižjimi časovnimi vrednostmi, pospeški vrhov in višinami skoka je bila akrobatska steza, ki mu je sledila tekmovalna velika ponjava in povečana velika ponjava, ti podatki lahko pomagajo uporabnikom in drugim, da razumejo vedenje športnikov pri skakanju

An improved DNA array-based classification method for the identification of Salmonella serotypes shows high concordance between traditional and genotypic testing.

Previously we developed and tested the Salmonella GenoSerotyping Array (SGSA), which utilized oligonucleotide probes for O- and H- antigen biomarkers to perform accurate molecular serotyping of 57 Salmonella serotypes. Here we describe the development and validation of the ISO 17025 accredited second version of the SGSA (SGSA v. 2) with reliable and unambiguous molecular serotyping results for 112 serotypes of Salmonella which were verified both in silico and in vitro. Improvements included an expansion of the probe sets along with a new classifier tool for prediction of individual antigens and overall serotype from the array probe intensity results. The array classifier and probe sequences were validated in silico to high concordance using 36,153 draft genomes of diverse Salmonella serotypes assembled from public repositories. We obtained correct and unambiguous serotype assignments for 31,924 (88.30%) of the tested samples and a further 3,916 (10.83%) had fully concordant antigen predictions but could not be assigned to a single serotype. The SGSA v. 2 can directly use bacterial colonies with a limit of detection of 860 CFU/mL or purified DNA template at a concentration of 1.0 x 10-1 ng/μl. The SGSA v. 2 was also validated in the wet laboratory and certified using panel of 406 samples representing 185 different serotypes with correct antigen and serotype determinations for 60.89% of the panel and 18.31% correctly identified but an ambiguous overall serotype determination

Canine detection of chronic wasting disease (CWD) in laboratory and field settings

ABSTRACTChronic wasting disease (CWD) is a fatal transmissible spongiform encephalopathy that affects both free-ranging and farmed cervid species, including mule deer, white-tailed deer, and elk (Odocoileus hemionus, Odocoileus virginianus, and Cervus canadensis). Due to the long incubation period and variability of clinical signs, CWD can expand and spread to new areas before they reach diagnostically detectable levels. Antemortem testing methods currently available can be difficult to obtain and to be applied to the large numbers required for adequate surveillance. However, key volatile biomarkers could be harnessed for non-invasive antemortem surveillance. Detection dogs are the most effective tool currently available for volatile detection; dogs can effectively complete wildlife surveys at rates surpassing that of humans. This study is the first to demonstrate that trained detection dogs can be used as an antemortem test for CWD. First, we trained three dogs to differentiate between CWD-positive and CWD-negative white-tailed deer faeces in a laboratory setting. Dogs spent significantly more time at the positive sample than the negative samples, suggesting that they differentiated between the positive and negative volatile signatures. We then trained the same dogs to search for CWD-positive faecal samples in a more naturalistic field setting. In the field, dogs found 8/11 CWD-positive samples and had an average false detection rate of 13%. These results suggest that dogs can be trained to differentiate CWD-positive faeces from CWD-negative faeces in both laboratory and field settings. Future studies will compare canine accuracy to other antemortem methods, as well as improved canine training methods

Multigenerational effects of bisphenol A or ethinyl estradiol exposure on F2 California mice (Peromyscus californicus) pup vocalizations.

Rodent pups use vocalizations to communicate with one or both parents in biparental species, such as California mice (Peromyscus californicus). Previous studies have shown California mice developmentally exposed to endocrine disrupting chemicals, bisphenol A (BPA) or ethinyl estradiol (EE), demonstrate later compromised parental behaviors. Reductions in F1 parental behaviors might also be due to decreased emissions of F2 pup vocalizations. Thus, vocalizations of F2 male and female California mice pups born to F1 parents developmentally exposed to BPA, EE, or controls were examined. Postnatal days (PND) 2-4 were considered early postnatal period, PND 7 and 14 were defined as mid-postnatal period, and PND 21 and 28 were classified as late postnatal period. EE pups showed increased latency to emit the first syllable compared to controls. BPA female pups had decreased syllable duration compared to control and EE female pups during the early postnatal period but enhanced responses compared to controls at late postnatal period; whereas, male BPA and EE pups showed greater syllable duration compared to controls during early postnatal period. In mid-postnatal period, F2 BPA and EE pups emitted greater number of phrases than F2 control pups. Results indicate aspects of vocalizations were disrupted in F2 pups born to F1 parents developmentally exposed to BPA or EE, but their responses were not always identical, suggesting BPA might not activate estrogen receptors to the same extent as EE. Changes in vocalization patterns by F2 pups may be due to multigenerational exposure to BPA or EE and/or reduced parental care received