Adultos Mayores y Memoria Local a Través de Lenguajes. Vivir para contarla – Relatos e identidad

El trabajo presenta un Proyecto de Extensión Universitaria en particular para pensar los aportes de la extensión a los procesos de creación y fortalecimiento de identidad a través de la recuperación de la memoria. El proyecto de Extensión Universitaria “Adultos mayores y memoria local a través de lenguajes” es llevado adelante en la Universidad Nacional de Quilmes y trabaja con centros de jubilados de la comunidad cercana a la Universidad. Mediante encuentros periódicos de reflexión, intercambio y diálogo, en los cuales van surgiendo historias para ser contadas mediante lenguajes mediáticos, especialmente en imagen (fotografía y video), pero también se realiza radio y textos para ser distribuidos por Internet. En estos mensajes los adultos mayores utilizan los puentes tendidos para relatar sus anécdotas pasadas, su realidad presente y, no pocas veces, sus proyectos a futuro. Podemos mencionar como trabajos realizados un ciclo audiovisual de relatos breves en primera persona titulado “Historias Mínimas”, una muestra fotográfica titulada “Historias Debidas”, La realización de un programa radial semanal llamado “La Voz del Centro Rosa y Celeste” y la filmación de un documental sobre el Centro de Jubilados Rincón de Amigos. Las narraciones de los adultos mayores quedan fuera de la televisión actual y de manera muy aislada se reconstruyen por el cine; los adultos mayores no son quienes generalmente protagonizan programas de radio masivos o quienes habitualmente escriben en las redes sociales virtuales, tan influyentes en la actualidad. Por ello creemos esencial su participación como contadores de historias tanto colectivas - locales o barriales- como personales. Los relatos permiten el reconocimiento de la posición de estos adultos mayores dentro del espacio simbólico de la cultura y la interacción entre todos los actores participantes favorecen las negociaciones (intercambios de experiencias y conocimientos originados en la práctica) necesarias para la identificación

Selective inhibition of genomic and non-genomic effects of thyroid hormone regulates muscle cell differentiation and metabolic behavior

Thyroid hormones (THs) are key regulators of different biological processes. Their action involves genomic and non-genomic mechanisms, which together mediate the final effects of TH in target tissues. However, the proportion of the two processes and their contribution to the TH-mediated effects are still poorly understood. Skeletal muscle is a classical target tissue for TH, which regulates muscle strength and contraction, as well as energetic metabolism of myofibers. Here we address the different contribution of genomic and non-genomic action of TH in skeletal muscle cells by specifically silencing the deiodinase Dio2 or the β3-Integrin expression via CRISPR/Cas9 technology. We found that myoblast proliferation is inversely regulated by integrin signal and the D2-dependent TH activation. Similarly, inhibition of the nuclear receptor action reduced myoblast proliferation, confirming that genomic action of TH attenuates proliferative rates. Contrarily, genomic and non-genomic signals promote muscle differentiation and the regulation of the redox state. Taken together, our data reveal that integration of genomic and non-genomic signal pathways finely regulates skeletal muscle physiology. These findings not only contribute to the understanding of the mechanisms involved in TH modulation of muscle physiology but also add insight into the interplay between different mechanisms of action of TH in muscle cells

Thyroid hormone enhances angiogenesis and the warburg effect in squamous cell carcinomas

Cancer angiogenesis is required to support energetic demand and metabolic stress, particu-larly during conditions of hypoxia. Coupled to neo-vasculogenesis, cancer cells rewire metabolic programs to sustain growth, survival and long-term maintenance. Thyroid hormone (TH) signaling regulates growth and differentiation in a variety of cell types and tissues, thus modulating hyper proliferative processes such as cancer. Herein, we report that TH coordinates a global program of metabolic reprogramming and induces angiogenesis through up-regulation of the VEGF-A gene, which results in the enhanced proliferation of tumor endothelial cells. In vivo conditional depletion of the TH activating enzyme in a mouse model of cutaneous squamous cell carcinoma (SCC) reduces the concentration of TH in the tumoral cells and results in impaired VEGF-A production and atten-uated angiogenesis. In addition, we found that TH induces the expression of the glycolytic genes and fosters lactate production, which are key traits of the Warburg effect. Taken together, our results reveal a TH–VEGF-A–HIF1α regulatory axis leading to enhanced angiogenesis and glycolytic flux, which may represent a target for SCC therapy

Tratamento da dermatofitose felina com o Lufenuron

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Usefulness of chlorezidine gluconate in 2% aqueous solution for treating bacterial pododermatitis in dogs

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Ocorrência de estágios larvais de Ancylostoma spp na areia de parques públicos infantis do município de Santos, Baixada Santista

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Multilocus genotyping reveals new molecular markers for differentiating distinct genetic lineages among “candidatus phytoplasma solani” strains associated with grapevine bois noir

Grapevine Bois noir (BN) is associated with infection by “Candidatus Phytoplasma solani” (CaPsol). In this study, an array of CaPsol strains was identified from 142 symptomatic grapevines in vineyards of northern, central, and southern Italy and North Macedonia. Molecular typing of the CaPsol strains was carried out by analysis of genes encoding 16S rRNA and translation elongation factor EF-Tu, as well as eight other previously uncharacterized genomic fragments. Strains of tuf-type a and b were found to be differentially distributed in the examined geographic regions in correlation with the prevalence of nettle and bindweed. Two sequence variants were identified in each of the four genomic segments harboring hlyC, cbiQ-glyA, trxA-truB-rsuA, and rplS-tyrS-csdB, respectively. Fifteen CaPsol lineages were identified based on distinct combinations of sequence variations within these genetic loci. Each CaPsol lineage exhibited a unique collective restriction fragment length polymorphism (RFLP) pattern and differed from each other in geographic distribution, probably in relation to the diverse ecological complexity of vineyards and their surroundings. This RFLP-based typing method could be a useful tool for investigating the ecology of CaPsol and the epidemiology of its associated diseases. Phylogenetic analyses highlighted that the sequence variants of the gene hlyC, which encodes a hemolysin III-like protein, separated into two clusters consistent with the separation of two distinct lineages on the basis of tufB gene sequences. Alignments of deduced full protein sequences of elongation factor-Tu (tufB gene) and hemolysin III-like protein (hlyC gene) revealed the presence of critical amino acid substitutions distinguishing CaPsol strains of tuf-type a and b. Findings from the present study provide new insights into the genetic diversity and ecology of CaPsol populations in vineyards