48 research outputs found
A model analysis of the photosynthetic response of Vitis vinifera L. cvs Riesling and Chasselas leaves in the field: I. Interaction of age, light and temperature
The photosynthetic activity (A) of leaves of different ages on primary and secondary shoots of Riesling and Chasselas vines was measured under field conditions in relation to photon flux density (PFD) at various leaf temperatures. The data sets from 4 years and two locations (Geisenheim, Germany; Changins, Switzerland) were analysed using non-linear regression models to determine possible genetic and/or climate-induced differences in the light and temperature response between different leaf ages. A non-rectangular hyperbola with physiologically meaningful parameters was found to adequately describe the response to photon flux density. For both varieties, maximum photosynthetic rates were observed on leaves of primary shoots, opposite to the clusters, at a leaf temperature of 27-32 °C and at light saturation. Young leaves showed a less pronounced temperature optimum. The light response curves of photosynthesis of the two cultivars were similar over a temperature range of 20-30 °C. Below this temperature, Riesling showed higher values of A than Chasselas in most cases, whereas it was the reverse when leaf temperature exceeded 30 °C. This was particularly evident for leaves on secondary shoots and was related to differences in the photorespiration rate. Mature Riesling leaves had higher apparent quantum yields (a) and lower light saturation indices (Is) than Chasselas at leaf temperatures below 30-35 °C. Dark respiration (RD) and the light compensation point (Ic) responded strongly to temperature with differences between leaf ages but no consistent difference between varieties. Leaves on secondary shoots of both cultivars had the highest photo-synthetic activity during the ripening period of the fruit
Carbohydrate reserves in grapevine (Vitis vinifera L. 'Chasselas'): the influence of the leaf to fruit ratio
Seasonal dynamics of total non-structural carbohydrates (TNC) in relation to the leaf-fruit ratio were measured over five years at different grapevine phenological stages in one- and two-year-old canes, trunks and roots of the cultivar 'Chasselas' (Vitis vinifera L.). Carbohydrates were mainly stored as starch in different parts of the grapevine during the growing season. Soluble carbohydrates represented only a small part (< 7 % of dry weight, DW) of the TNC. In the roots and trunks, the starch content fluctuated during the growing season, reaching the lowest values between budbreak and flowering depending on the year, and the highest values between harvest and leaf fall. The soluble sugar content increased in the trunks and the two-year-old canes during the winter period with the decrease in temperatures. A negative correlation was established between the average air temperature recorded during the seven days before sample collection for carbohydrate analysis, and soluble carbohydrate content in the trunks and two-year-old canes. The leaffruit ratio (source-sink), expressed by the “light-exposed leaf area∙kg-1 fruit”, not only substantially influenced the soluble sugar content in berries but also the starch and TNC concentrations in the trunks and roots at harvest. Higher leaf-fruit ratios resulted in increased starch and TNC concentrations in the trunks and roots, which attained the maximum values when the leaf-fruit ratio neared 2.0 m2 of light-exposed leaf area∙kg-1 fruit. Canopy height and leaf area had no predominant influence on the soluble sugars, starch contents, or TNC in the permanent vine parts.
Manejo do dossel vegetativo e seu efeito nos componentes de produção da videira Merlot.
A poda verde é uma prática cultural utilizada para melhorar as condições do dossel vegetativo dos vinhedos, visando a favorecer a qualidade da uva e do vinho. Nesse sentido, realizou-se este experimento entre as safras de 1993/1994 e 1996/1997, com diferentes modalidades de poda verde, num vinhedo do cv. Merlot conduzido em latada. Houve 12 tratamentos e três repetições, sendo o delineamento experimental em blocos casualizados. Os tratamentos constituíram-se da testemunha e de 11 diferentes modalidades de poda verde, ou seja, desbrota, desponta e desfolha, algumas delas em diferentes épocas do ciclo vegetativo da videira. O componente principal 1, da análise de componentes principais (ACP) feita em cada ano, separadamente, mostra que o tratamento 10 (desbrota + desponta + desfolha realizada no início da floração, eliminando-se as folhas abaixo dos cachos) discriminou-se nos quatro anos, e os tratamentos 7 (desfolha realizada 21 dias antes da colheita, eliminando-se metade das folhas abaixo dos cachos) e 6 (desfolha realizada 21 dias antes da colheita, eliminando-se as folhas abaixo dos cachos), em três deles; a ACP da média dos quatro anos também evidencia essa discriminação entre eles. Constata-se que o tratamento 10 foi um dos que tiveram intensidade de poda verde mais intensa, caracterizando-se por variáveis indicativas de plantas com vigor e produtividade mais baixos que os demais
Intensidades da poda seca e do desbaste de cacho na composição da uva Cabernet Sauvignon.
A pesquisa foi realizada durante quatro anos, num vinhedo de Cabernet Sauvignon (Vitis vinifera L.), em Bento Gonçalves-RS. O objetivo foi determinar o efeito das intensidades da poda seca e do desbaste de cacho em variáveis relacionadas aos componentes de produção da videira e à composição do mosto de uva. Os tratamentos consistiram em dois níveis de poda seca - curta e longa - e quatro de desbaste de cacho (%) - 0; 25; 50 e 75 -; com cinco repetições. O delineamento experimental foi o em blocos casualizados, com os tratamentos em parcelas subdivididas. Os resultados mostram que a poda seca e o desbaste de cacho tiveram efeito altamente significativo na produtividade do vinhedo que, na média dos quatro anos, variou de 10.971 kg/ha ? poda curta, 75% de desbaste ? a 32.819 kg/ha ? poda longa, 0% de desbaste. Houve, também, efeito significativo na produtividade por gema, peso de ramos podados por gema e por hectare, área foliar/peso fresco do fruto e produtividade/peso de ramos podados. Entretanto, o efeito nas variáveis relacionadas a açúcar e acidez do mosto da uva foi pouco expressivo. O componente 1 da análise de componentes principais discriminou o tratamento poda curta a 75% de desbaste de cacho dos tratamentos poda longa-0% de desbaste e poda longa-25% de desbaste
New Insights into the Evolution of Metazoan Tyrosinase Gene Family
Tyrosinases, widely distributed among animals, plants and fungi, are involved in the biosynthesis of melanin, a pigment that has been exploited, in the course of evolution, to serve different functions. We conducted a deep evolutionary analysis of tyrosinase family amongst metazoa, thanks to the availability of new sequenced genomes, assessing that tyrosinases (tyr) represent a distinctive feature of all the organisms included in our study and, interestingly, they show an independent expansion in most of the analyzed phyla. Tyrosinase-related proteins (tyrp), which derive from tyr but show distinct key residues in the catalytic domain, constitute an invention of chordate lineage. In addition we here reported a detailed study of the expression territories of the ascidian Ciona intestinalis tyr and tyrps. Furthermore, we put efforts in the identification of the regulatory sequences responsible for their expression in pigment cell lineage. Collectively, the results reported here enlarge our knowledge about the tyrosinase gene family as valuable resource for understanding the genetic components involved in pigment cells evolution and development