Certain Adenylated Non-Coding RNAs, Including 5′ Leader Sequences of Primary MicroRNA Transcripts, Accumulate in Mouse Cells following Depletion of the RNA Helicase MTR4

RNA surveillance plays an important role in posttranscriptional regulation. Seminal work in this field has largely focused on yeast as a model system, whereas exploration of RNA surveillance in mammals is only recently begun. The increased transcriptional complexity of mammalian systems provides a wider array of targets for RNA surveillance, and, while many questions remain unanswered, emerging data suggest the nuclear RNA surveillance machinery exhibits increased complexity as well. We have used a small interfering RNA in mouse N2A cells to target the homolog of a yeast protein that functions in RNA surveillance (Mtr4p). We used high-throughput sequencing of polyadenylated RNAs (PA-seq) to quantify the effects of the mMtr4 knockdown (KD) on RNA surveillance. We demonstrate that overall abundance of polyadenylated protein coding mRNAs is not affected, but several targets of RNA surveillance predicted from work in yeast accumulate as adenylated RNAs in the mMtr4KD. microRNAs are an added layer of transcriptional complexity not found in yeast. After Drosha cleavage separates the pre-miRNA from the microRNA\u27s primary transcript, the byproducts of that transcript are generally thought to be degraded. We have identified the 5′ leading segments of pri-miRNAs as novel targets of mMtr4 dependent RNA surveillance

Maximum likelihood estimation of photon number distribution from homodyne statistics

We present a method for reconstructing the photon number distribution from the homodyne statistics based on maximization of the likelihood function derived from the exact statistical description of a homodyne experiment. This method incorporates in a natural way the physical constraints on the reconstructed quantities, and the compensation for the nonunit detection efficiency.Comment: 3 pages REVTeX. Final version, to appear in Phys. Rev. A as a Brief Repor

Substitution induced pinning in MgB_2 superconductor doped with SiC nano-particles

By doping MgB_2 superconductor with SiC nano-particles, we have successfully introduced pinning sites directly into the crystal lattice of MgB_2 grains (intra-grain pinning). It became possible due to the combination of counter-balanced Si and C co-substitution for B, leading to a large number of intra-granular dislocations and the dispersed nano-size impurities induced by the substitution. The magnetic field dependence of the critical current density was significantly improved in a wide temperature range, whereas the transition temperature in the sample MgB_2(SiC)_x having x = 0.34, the highest doping level prepared, dropped only by 2.6 K.Comment: 4 pages, 6 figure

Multi-mode density matrices of light via amplitude and phase control

A new method is described for determining the quantum state of correlated multimode radiation by interfering the modes and measuring the statistics of the superimposed fields in four-port balanced homodyne detection. The full information on the $N$-mode quantum state is obtained by controlling both the relative amplitudes and the phases of the modes, which simplifies the reconstruction of density matrices to only $N+1$ Fourier transforms. In particular, this method yields time-correlated multimode density matrices of optical pulses by superimposing the signal by a sequence of short local-oscillator pulses.Comment: 6 pages, late

Reply on the ``Comment on `Loss-error compensation in quantum- state measurements' ''

The authors of the Comment [G. M. D'Ariano and C. Macchiavello to be published in Phys. Rev. A, quant-ph/9701009] tried to reestablish a 0.5 efficiency bound for loss compensation in optical homodyne tomography. In our reply we demonstrate that neither does such a rigorous bound exist nor is the bound required for ruling out the state reconstruction of an individual system [G. M. D'Ariano and H. P. Yuen, Phys. Rev. Lett. 76, 2832 (1996)].Comment: LaTex, 2 pages, 1 Figure; to be published in Physical Review

Operational Theory of Homodyne Detection

We discuss a balanced homodyne detection scheme with imperfect detectors in the framework of the operational approach to quantum measurement. We show that a realistic homodyne measurement is described by a family of operational observables that depends on the experimental setup, rather than a single field quadrature operator. We find an explicit form of this family, which fully characterizes the experimental device and is independent of a specific state of the measured system. We also derive operational homodyne observables for the setup with a random phase, which has been recently applied in an ultrafast measurement of the photon statistics of a pulsed diode laser. The operational formulation directly gives the relation between the detected noise and the intrinsic quantum fluctuations of the measured field. We demonstrate this on two examples: the operational uncertainty relation for the field quadratures, and the homodyne detection of suppressed fluctuations in photon statistics.Comment: 7 pages, REVTe

LOW ENERGY SUPERSYMMETRY PHENOMENOLOGY

We summarize the current status and future prospects for low energy (weak scale) supersymmetry. In particular, we evaluate the capabilities of various $e^+e^-$, $p\bar p$ and $pp$ colliders to discover evidence for supersymmetric particles. Furthermore, assuming supersymmetry is discovered, we discuss capabilities of future facilities to dis-entangle the anticipated spectrum of super-particles, and, via precision measurements, to test mass and coupling parameters for comparison with various theoretical expectations. We comment upon the complementarity of proposed hadron and $e^+e^-$ machines for a comprehensive study of low energy supersymmetry.Comment: 74 page (Latex) file; a PS or uuencoded manuscript with embedded figures is available via anonymous ftp at ftp://hep.fsu.edu/preprints/baer/FSUHEP950401.ps or .uu . Contributed chapter to DPF study group on Electroweak Symmetry Breaking and Beyond the Standard Model

Doping Effect of Nano-Diamond on Superconductivity and Flux Pinning in MgB2

Doping effect of diamond nanoparticles on the superconducting properties of MgB2 bulk material has been studied. It is found that the superconducting transition temperature Tc of MgB2 is suppressed by the diamond-doping, however, the irreversibility field Hirr and the critical current density Jc are systematically enhanced. Microstructural analysis shows that the diamond-doped MgB2 superconductor consists of tightly-packed MgB2 nano-grains (~50-100 nm) with highly-dispersed and uniformly-distributed diamond nanoparticles (~10-20 nm) inside the grains. High density of dislocations and diamond nanoparticles may take the responsibility for the enhanced flux pinning in the diamond-doped MgB2.Comment: 16 pages, 6 figure

Women with diabetes are at increased relative risk of heart failure compared to men: Insights from UK Biobank

Aims: To investigate the effect of diabetes on mortality and incident heart failure (HF) according to sex, in the low risk population of UK Biobank. To evaluate potential contributing factors for any differences seen in HF end-point. Methods: The entire UK Biobank study population were included. Participants that withdrew consent or were diagnosed with diabetes after enrolment were excluded from the study. Univariate and multivariate cox regression models were used to assess endpoints of mortality and incident HF, with median follow-up periods of 9 years and 8 years respectively. Results: A total of 493,167 participants were included, hereof 22,685 with diabetes (4.6%). Two thousand four hundred fifty four died and 1,223 were diagnosed or admitted with HF during the follow up periods of 9 and 8 years respectively. Overall, the mortality and HF risk were almost doubled in those with diabetes compared to those without diabetes (hazard ratio (HR) of 1.9 for both mortality and heart failure) in the UK Biobank population. Women with diabetes (both types) experience a 22% increased risk of HF compared to men (HR of 2.2 (95% CI: 1.9-2.5) vs. 1.8 (1.7-2.0) respectively). Women with type 1 diabetes (T1DM) were associated with 88% increased risk of HF compared to men (HR 4.7 (3.6-6.2) vs. 2.5 (2.0-3.0) respectively), while the risk of HF for type 2 diabetes (T2DM) was 17% higher in women compared to men (2.0 (1.7-2.3) vs. 1.7 (1.6-1.9) respectively). The increased risk of HF in women was independent of confounding factors. The findings were similar in a model with all-cause mortality as a competing risk. This interaction between sex, diabetes and outcome of HF is much more prominent for T1DM (p = 0.0001) than T2DM (p = 0.1). Conclusion: Women with diabetes, particularly those with T1DM, experience a greater increase in risk of heart failure compared to men with diabetes, which cannot be explained by the increased prevalence of cardiac risk factors in this cohort