Mediation of the uncoupled eNOS pathway following oxidative stress using tetrahydrobiopterin and nitric oxide donor drugs to restore tetrahydrobiopterin concentration

Presentation Location: Warming House, Olivet Nazarene University Abstract The eNOS pathway, found in the endothelium of blood vessels, is a key regulator of nitric oxide levels in the circulatory system. The pathway is controlled through several positive and negative feedback loops [2]. The cofactor tetrahydrobiopterin (BH4) is a major control point in this pathway and under conditions of stress can be reduced into the dihydrobiopterin (BH2) [2,6,7,8,9]. When the reduced form is predominant, the pathway produces reactive oxygen species (ROS) rather than nitric oxide, causing stress and damage to the vessels [6,7,8,9]. In this study, different treatments were studied to determine which is most effective in restoring BH4 levels in the eNOS pathway of bovine aortic endothelial cells (BAECs). Nitric oxide supplementation was the main focus of this study and was tested as a stand-alone treatment and as a combined treatment along with a BH4 donor drug, sapropterin dihydrochloride. Following the two treatments, only the BAECs given the nitric oxide donor drug showed levels of BH4 higher than the untreated control cells. The cells treated with 25 mM nitric oxide donor drug and 2.5 mM BH4 donor drug showed levels of BH4 that were most similar to the untreated control cells with a concentration of approximately 27 mM BH4

Study of Blood Viscosity with Added Sodium Nitrate and Temperature Variance: A Potential Therapy to Regulate Blood Flow After Induced Hypothermia

The human body has natural systems for vasodilation which are fueled by nitric oxide production, but in cases of cardiac disfunction and stress nitric oxide can be inhibited. In this study, nitric oxide was studied as a mediator for the blood rush experienced from the warming after induced hypothermia. Nitric oxide (NO) was introduced through sodium nitrate, which was aimed to reduce the speed and turbulence of blood flow through interaction between NO and the active site of hemoglobin. A viscometer was used to examine the rate of blood flow, while the temperature was varied to simulate the conditions of induced hypothermia. Two temperatures were tested to simulate the warming following induced hypothermia, and three concentrations of sodium nitrate were added to the samples to examine the correlation between flow and concentration. The results indicated that the 0.11 M NaNO3 expressed constant blood viscosity for both temperatures, the 0.09 M NaNO3 exhibited some mediation, and the 0.10 M showed the least mediation

Study of Blood Viscosity with Added Sodium Nitrate and Temperature Variance: A Potential Therapy to Regulate Blood Flow After Induced Hypothermia

The human body has natural systems for vasodilation which are fueled by nitric oxide production, but in cases of cardiac disfunction and stress nitric oxide can be inhibited. In this study, nitric oxide was studied as a mediator for the blood rush experienced from the warming after induced hypothermia. Nitric oxide (NO) was introduced through sodium nitrate, which was aimed to reduce the speed and turbulence of blood flow through interaction between NO and the active site of hemoglobin. A viscometer was used to examine the rate of blood flow, while the temperature was varied to simulate the conditions of induced hypothermia. Two temperatures were tested to simulate the warming following induced hypothermia, and three concentrations of sodium nitrate were added to the samples to examine the correlation between flow and concentration. The results indicated that the 0.11 M NaNO3 expressed constant blood viscosity for both temperatures, the 0.09 M NaNO3 exhibited some mediation, and the 0.10 M showed the least mediation

Study of Blood Viscosity with Added Sodium Nitrate and Temperature Variance: A Potential Therapy to Regulate Blood Flow After Induced Hypothermia

Recorded presentation. The human body has natural systems for vasodilation which are fueled by nitric oxide production, but in cases of cardiac disfunction and stress nitric oxide can be inhibited. In this study, nitric oxide was studied as a mediator for the blood rush experienced in the warming process of induced hypothermia after a major cardiac event. Nitric oxide (NO) was introduced through sodium nitrate which was aimed to reduce the speed and turbulence of blood flow. A viscometer was used to examine the rate of blood flow, while the temperature was varied to simulate the conditions of induced hypothermia. Two temperatures were tested to simulate the warming process of induced hypothermia, and three concentrations of sodium nitrate were added to the samples. A control was used for comparison and for calculations. The results indicated that the 0.11 M NaNO3 expressed constant blood viscosity for both temperatures, the 0.09 M NaNO3 exhibited some mediation and the 0.10 M showed the least mediation

Playing Devil’s Advocate: A Conceptual Replication of Hirt et al. (2003)

In the field of psychology, it is important that we foster unbiased judgments in our students and encourage them to engage with material on a critical level. The proposed study is a conceptual replication of Hirt et al. (2003), investigating whether considering alternative hypotheses about the results reduces explanation and overconfidence biases for questions about psychological research. Our proposed replication will also consider whether the benefits of counterfactual thinking were transferable, meaning once individuals are prompted to think counterfactually in one domain, they should show reduced biases when considering an unrelated domain. If these debiasing effects generalize to psychological information, and show transfer across a variety of psychological research, they could play an important role in training psychology students

Pre-Anesthetic Evaluation: A Needs Assessment of Student Registered Nurse Anesthetists Transitioning to Clinical Practice

The pre-anesthetic evaluation (PAE) is a critical part of providing anesthesia, and an important component of patient safety. Student registered nurse anesthetists (SRNAs) are often required to independently perform the PAE upon entering their clinical training. Stress, anxiety, doubt, a novel environment and time constraints can lead to a lack of confidence and competence at this time. These barriers can result in an inefficient and inadequate assessment. The purpose of this qualitative study was to conduct a needs assessment to determine the overall readiness of SRNAs to independently perform a thorough PAE upon entering clinical residency. A focus group was conducted and qualitative data software was used for thematic analysis. Four main themes were identified from the focus group data. These were barriers to performing the PAE, emotions experienced, facilitators for performing the PAE, and the SRNA’s experience of their role transition. The ability to practice the PAE prior to entering clinical residency as well as using a reference guide are beneficial to SRNAs as they start their clinical residency. These tools can help decrease stress and anxiety allowing for a more consistent, thorough, and efficient PAE

Assessment of Spatial Location and Air Transport Patterns of Minnesota’s Medical Device Industry Cluster

Minnesota’s medical device industry cluster is not only one of the biggest driving economic forces in the state, but also a global leader in the development and distribution of medical devices. The study assessed the medical device industry’s spatial development and air transport implications, both in terms of industry cluster location and flow of products. The spatial analysis showed that most medical device companies are located within the seven-county Twin-Cities region, but that industries linked to the medical device industry cluster are much more dispersed throughout the state. Regarding products created, the supply chain of medical devices is highly dependent on Minneapolis-St Paul Airport (MSP), which plays a key role in their delivery of medical devices. Air Cargo analysis reveals the high value of medical devices. exports to various locations and raises issues about the future role of air cargo through MSP to the economic prosperity of the industry cluster