700 research outputs found
The role of the charge state of PAHs in ultraviolet extinction
Aims: We explore the relation between charge state of polycyclic aromatic
hydrocarbons (PAHs) and extinction curve morphology. Methods: We fit extinction
curves with a dust model including core-mantle spherical particles of mixed
chemical composition (silicate core, and carbonaceous layers),
and an additional molecular component. We use exact methods to calculate the
extinction due to classical particles and accurate computed absorption spectra
of PAHs in different charge states, for the contribution due to the molecular
component, along a sample of five rather different lines of sight. Results: A
combination of classical dust particles and mixtures of real PAHs
satisfactorily matches the observed interstellar extinction curves. Variations
of the spectral properties of PAHs in different charge states produce changes
consistent with the varying relative strengths of the bump and non-linear
far-UV rise.Comment: 5 pages, 3 figures, Astronomy & Astrophysics Letters, in pres
NODAL Secures Pluripotency upon Embryonic Stem Cell Progression from the Ground State
Naive mouse embryonic stem cells (ESCs) can develop multiple fates, but the cellular and molecular processes that enable lineage competence are poorly characterized. Here, we investigated progression from the ESC ground state in defined culture. We utilized downregulation of Rex1::GFPd2 to track the loss of ESC identity. We found that cells that have newly downregulated this reporter have acquired capacity for germline induction. They can also be efficiently specified for different somatic lineages, responding more rapidly than naive cells to inductive cues. Inhibition of autocrine NODAL signaling did not alter kinetics of exit from the ESC state but compromised both germline and somatic lineage specification. Transient inhibition prior to loss of ESC identity was sufficient for this effect. Genetic ablation of Nodal reduced viability during early differentiation, consistent with defective lineage specification. These results suggest that NODAL promotes acquisition of multi-lineage competence in cells departing naive pluripotency.This research was funded by the Wellcome Trust (091484/Z/10/Z). The Cambridge Stem Cell Institute receives core support from the Wellcome Trust and the Medical Research Council (G1100526). C.M. was funded by a BBSRC studentship (961424). A.S. is a Medical Research Council Professor
Laboratory studies of polycyclic aromatic hydrocarbons: the search for interstellar candidates
Polycyclic Aromatic Hydrocarbons (PAHs) are considered as a major constituent
of interstellar dust. They have been proposed as the carriers of the Aromatic
Infrared Bands (AIBs) observed in emission in the mid-IR. They likely have a
significant contribution to various features of the extinction curve such as
the 220 nm bump,the far-UV rise and the diffuse interstellar bands. Emission
bands are also expected in the far-IR, which are better fingerprints of
molecular identity than the AIBs. They will be searched for with the Herschel
Space Observatory. Rotational emission is also expected in the mm range for
those molecules which carry significant dipole moments. Despite spectroscopic
studies in the laboratory, no individual PAH species could be identified. This
emphasises the need for an investigation on where interstellar PAHs come from
and how they evolve due to environmental conditions: ionisation and
dissociation upon UV irradiation, interactions with electrons, gas and dust.
There is also evidence for PAH species to contribute to the depletion of heavy
atoms from the gas phase, in particular Si and Fe. This paper illustrates how
laboratory work can be inspired from observations. In particular there is a
need for understanding the chemical properties of PAHs and PAH-related species,
including very small grains, in physical conditions that mimic those found in
interstellar space. This motivates a joint effort between astrophysicists,
physicists and chemists. Such interdisciplinary studies are currently
performed, taking advantage of the PIRENEA set-up, a cold ion trap dedicated to
astrochemistry.Comment: to appear in "Cosmic Dust - Near and Far", Th. Henning, E. Grun, J.
Steinacker (eds.
Reproductive aspects of the velvet belly lantern shark Etmopterus spinax (Condrichthyes: Etmopteridae), from the central western Mediterranean sea. Notes on gametogenesis and oviducal gland microstructure.
In this paper, the reproductive biology of the velvet belly lanternshark Etmopterus spinax was analyzed in Sardinian waters (central western Mediterranean). This species was sexually dimorphic with females growing to a larger size than males. Marked sexual dimorphism in size was also observed along the vertical gradient. Histological analysis of gonads was very useful in assigning macroscopical maturity stages. The investigation on the microstructure of oviducal gland (OG) highlighted four morphofunctional zones with mucous and/or proteic secretions according to the zone and to their specific functions and development. Sperm in the OG was found for the first time in E. spinax. The localization of sperm storage tubules deeper in OG suggested long-term sperm storage, which is in agreement with the long reproductive cycle described. This species matured late, specifically at 80.7% and 79% at the maximum observed size for females and males respectively. Mature specimens were found throughout the year with pregnant females observed in winter and autumn. A low fecundity was observed with a mean ovarian fecundity of 16.5 mature follicles
Correction: Defined conditions for propagation and manipulation of mouse embryonic stem cells (doi:10.1242/dev.173146).
A taxonomic survey of female oviducal glands in chondrichthyes: A comparative overview of microanatomy in the two reproductive modes
Oviducal glands (OGs) are distinct expanded regions of the anterior portion of the oviduct, commonly found in chondrichthyans, which play a key role in the production of the egg in-vestments and in the female sperm storage (FSS). The FSS phenomenon has implications for understanding the reproductive ecology and management of exploited populations, but little information is available on its taxonomic extent. For the first time, mature OGs from three lecithotrophic oviparous and four yolk-sac viviparous species, all considered at risk from the fishing impacts in the central western Mediterranean Sea, were examined using light microscopy. The OG microanatomy, whose morphology is generally conserved in all species, shows differences within the two reproductive modalities. Oviparous species show a more developed baffle zone in respect to viviparous ones because of the production of different egg envelopes produced. Among oviparous species, Raja polystigma and Chimaera monstrosa show presence of sperm, but not sperm storage as observed, instead, in Galeus melastomus and in all the viviparous sharks, which preserve sperm inside of specialized structures in the terminal zone
Polycyclic aromatic hydrocarbons with SPICA
Thanks to high sensitivity and angular resolution and broad spectral
coverage, SPICA will offer a unique opportunity to better characterize the
nature of polycyclic aromatic hydrocarbons (PAHs) and very small grains (VSGs),
to better use them as probes of astrophysical environments. The angular
resolution will enable to probe the chemical frontiers in the evolution process
from VSGs to neutral PAHs, to ionized PAHs and to "Grand-PAHs" in
photodissotiation regions and HII regions, as a function of G/n (UV
radiation field / density). High sensitivity will favor the detection of the
far-IR skeletal emission bands of PAHs, which provide specific fingerprints and
could lead to the identification of individual PAHs. This overall
characterization will allow to use PAH and VSG populations as tracers of
physical conditions in spatially resolved protoplanetary disks and nearby
galaxies (using mid-IR instruments), and in high redshift galaxies (using the
far-IR instrument), thanks to the broad spectral coverage SPICA provides. Based
on our previous experience with ISO and Spitzer we discuss how these goals can
be reached.Comment: To appear in Proc. Workshop "The Space Infrared Telescope for
Cosmology & Astrophysics: Revealing the Origins of Planets and Galaxies".
Eds. A.M. Heras, B. Swinyard, K. Isaak, and J.R. Goicoeche
New insights on the systematics of echinoids belonging to the family Spatangidae Gray, 1825 using a combined approach based on morphology, morphometry, and genetics
Spatangoids are probably the least resolved group within echinoids, with known topological incongruencies between phylogenies derived from molecular (very scarce) and morphological data. The present work, based on the analysis of 270 specimens of Spatangidae (Echinoidea, Spatangoida) trawled in the Sardinian seas (Western Mediterranean), allowed us to verify the constancy of some characters that we consider to be diagnostic at the genus level —such as the path of the subanal fasciole and the relationship between labrum and adjacent ambulacral plates —and to distinguish two distinct forms within the studied material. Based on morphological characters, morphometrics, and molecular analyses (sequencing of two mitochondrial markers: cytochrome c oxidase subunit1 (COI) and 16S), most of the individuals were classified as morphotype A and attributed to the species Spatangus purpureus, the most common spatangoid in the Mediterranean Sea, while a few corresponded to a different morphotype (B), genetically close to the species Spatangus raschi. Preliminary morphological analyses seemed to indicate that morphotype B specimens from Sardinia are slightly different from S. raschi and from Spatangus subinermis individuals, the second species of the family known to occur in the Mediterranean Sea. On the basis of morpho-structural observations and molecular analyses, comparing Mediterranean living forms with species from other areas (Central Eastern Atlantic, North Sea and neighboring basins, South African Sea, Philippines and Indonesian Archipelago, New Zealand, and Hawaiian Islands), the clear distinction of S. purpureus from several other species classified as Spatangus was confirmed. Based on the morphological and genetic differences, we propose to maintain the genus Spatangus including in it only the type species S. purpureus among the living species and to establish the new genus Propespatagus nov. gen. to include several other species previously classified as Spatangus. The clear distinction among different genera was also detected in fossil forms of Spatangus, Propespatagus nov. gen., and Sardospatangus (†) from the European Oligo-Miocene sedimentary rocks of Germany; the Miocene of Ukraine, Italy, and North Africa; the Plio-Pleistocene of Italy; and the Mio-Pliocene of Florida (USA). The new data can help in addressing taxonomic ambiguities within echinoids, as well as in improving species identification, and hence biodiversity assessments in the Mediterranean region
- …