Petroleum Product as Substrate for the Production of Biosurfactive Amphiphlies by Pseudomonas Aeruginosa

Biosurfactant production by Pseudomonas aeruginosa by utilizing diesel as the source of energy and carbon was studied in this research. Biosurfactant-producing strain of the organism was isolated from hydrocarbon-contaminated water and grown in a mineral medium supplemented with diesel. Production of biosurfactant was assayed by monitoring the increase in cell concentration, biosurfactant concentration, emulsification index and decrease in surface tension. Highest level of cell concentration and biosurfactant concentration (3.3^108CFU/ml and 0.0108mg/ml respectively) were obtained at 132hrs. Emulsification index reached its maximum with 33.3% at 108hrs. Surface tension reached its minimum at 120hrs with a value of 1.1^10-6N/m. The results showed that diesel can be utilized by P. aeruginosa to produce biosurfactant and early stationary phase isolates can be used to obtain higher yield. It also revealed the increasing potentiality of microorganisms in the aspect of oil spill cleanup and rapid reclamation of contaminated lands and water bodies.Keywords: Biosurfactant, diesel, surface tension, emulsification index, cell biomass, Pseudomonas aeruginosa

Influence of Periodic Administration of Garlic Extract on Blood Parameters of Grazing Lambs

The study was carried out to investigate the effects of periodic  administration of garlic extract drench on haematology and serum biochemistry of grazing lambs. Twelve West African dwarf sheep with an average weight of 12.00 ±0.77 kg were allotted to 3 treatment groups with 4 animals per group in a completely randomized design which lasted for 10 weeks. Treatment 1 (T1) served as control with no garlic extract, Treatment (T2) were given 5ml garlic extract weekly and Treatment T3 received 5ml garlic extract every 2 weeks. Results of haematological analysis showed that PCV significantly (P<0.05) differs among treatment groups while RBC, MCV, WBC, LYM and GRA all show no significant (P>0.05) difference. The lowest PCV of 19.68% was observed in T1 while T2 recorded the highest PCV of 29.68%. The results from serum biochemical indices shows that cholesterol and ALT differ significantly (P<0.05) among treatment groups while total protein, albumin, globulin, glucose, urea and AST all show no significant (P>0.05) difference among treatment means. The lowest serum cholesterol value of 0.78 was observed in T2 while the control group  recorded the highest value of 1.30. The study suggests that garlic extract could be used to reduce the level of serum cholesterol in grazing lamb thereby improving meat quality.Keywords: Garlic Extract, West African Dwarf Sheep, Haematology, Biochemistry

Impact of Biofilms on Water Distribution System of a Tertiary Institution in Northern Nigeria

The aim of this research was to determine the impact of biofilms on water distribution system of FederalUniversity Birnin Kebbi. Samples were collected from different water distribution pipelines in the University.Total heterotrophic count was carried out to determine the microbial load and Most Probable Method (MPN)was used to detect the presence of fecal coliforms in the water. Total heterotrophic bacterial count rangedbetween 1.6×103 to 3.9×103 cfu/ml. Escherichia Coli showed the highest frequency (25%) of occurrence, whilethe least frequency of occurrence (5%) was recorded for Klebsiella spp and Enterobacter spp respectively. Theisolates identified were Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp,Enterobacteria Spp, Salmonella Spp and Bacillus Spp. Presence of these isolates is of significant concern andmay cause some water borne diseases like diarrhea, dysentery etc. It is therefore recommended that water boardtreatment plant should use disinfectant chemicals like chlorine and perform regular proactive preventivemaintenance, microbial monitoring and infrastructure replacement and repair so as to reduce the occurrence ofbiofilms in the Water Distribution system

Phytochemical and antibacterial properties of garlic extracts

The antibacterial potency of aqueous and methanol extracts of garlic was determined invitro against three bacterial isolates (Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa) by agar well  diffusion method. Preliminary phytochemical screening revealed the presence of tannin, flavonoid, carbohydrate, protein, saponin, alkaloid and glycoside in the plants while anthraquinone was absent. Both the aqueous and methanol extract of garlic were observed to be more potent against E. coli with maximum zone of growth inhibition of 21.5mm at 200mg/ml and 24.0mm at 200mg/ml respectively. The minimum inhibitory concentration (MIC) of the aqueous and methanol extracts of garlic against E. coli was 100mg/ml and 50mg/ml respectively, and for S. aureus it was 200mg/ml and 100mg/ml respectively. Similarly, The MIC against Pseudomonas aeruginosa for the aqueous and methanol extracts was 200mg/ml and 200mg/ml respectively. Higher minimum bactericidal concentration (MBC) of 300mg/ml was observed against P. aeruginosa with aqueous extract and 250mg/ml with the methanol extract. The MBC for both the aqueous and methanol extract was 200mg/ml respectively against E. coli while MBC of 300mg/ml was observed against S. aureus for the aqueous extract and 200mg/ml for the methanol extract. These findings therefore justify the traditional medicinal use of garlic.Keywords; Phytochemical, Antibacterial, Efficacy, Garlic, Isolates

Studies on Haematology and Serum Biochemistry of Broiler Chickens Finished on an Unprocessed and Processed Velvet Bean (Mucuna Pruriens (L.)) as Dietary Protein Sources

The effects of different processed velvet seed on haematology and serum indices of Broiler chickens was studied using 150 four weeks old broiler chicks. The birds were randomly assigned to five treatment groups of 30 birds with three replications of 10 birds each. The chickens were finished on five dietary treatments formulated to contain 5% raw, soaked and boiled, cracked and boiled and roasted velvet bean seed meal as protein source in diets 1, 2, 3, 4 and 5 respectively. The haematological parameters indicated no significant difference (P> 0.05) among treatment groups for PCV, Hb, RBC, WBC, MCHC and PLT while MCV and MCH differ significantly (P< 0.05) where chickens on treatment T3 (boiled) had the highest values of 133.33 fl and 42.80 pg while lower values were chickens in T4 (cracked and boiled) 123.90 fl and 39.80 pg as the lowest. Urea concentration range of 7.88-10.08 mmol/l obtained with significant difference (P< 0.05) among treatment groups with T5(roasted) having value of 10.08 mmol/l and T2 (soaked) having least value of sodium 7.88 mmol/l. Serum electrolytes such as Sodium (Na+), Chloride (Cl-) and bicarbonate showed significant (P< 0.05) difference among treatment groups. The study concluded that, on account of adequate haematocrit and immune statuses, in addition to its hypoglycaemic ability, boiling mucuna seed meal with 5.00 % level of inclusion can be used without any deleterious effect on haematological and serum biochemical assay parameters of broiler chickens.Keywords: Velvet bean seeds, Haematology, Biochemistry Assay, Carcass Characteristic

Influence of Periodic Administration of Garlic Extract on Blood Parameters of Grazing Lambs

The study was carried out to investigate the effects of periodic administration of garlic extract drench on haematology and serum biochemistry of grazing lambs. Twelve West African dwarf sheep with an average weight of 12.00 \ub10.77 kg were allotted to 3 treatment groups with 4 animals per group in a completely randomized design which lasted for 10 weeks. Treatment 1 (T1) served as control with no garlic extract, Treatment (T2) were given 5ml garlic extract weekly and Treatment T3 received 5ml garlic extract every 2 weeks. Results of haematological analysis showed that PCV significantly (P<0.05) differs among treatment groups while RBC, MCV, WBC, LYM and GRA all show no significant (P>0.05) difference. The lowest PCV of 19.68% was observed in T1 while T2 recorded the highest PCV of 29.68%. The results from serum biochemical indices shows that cholesterol and ALT differ significantly (P<0.05) among treatment groups while total protein, albumin, globulin, glucose, urea and AST all show no significant (P>0.05) difference among treatment means. The lowest serum cholesterol value of 0.78 was observed in T2 while the control group recorded the highest value of 1.30. The study suggests that garlic extract could be used to reduce the level of serum cholesterol in grazing lamb thereby improving meat quality

Association of Chlamydia serology with HIV in Nigerian women

This research was carried out to detect the presence of Chlamydia in pregnant women and gynaecologic patients in the North-central geopolitical zone of Nigeria. Blood samples were collected and analysed by ELISA techniques. The blood samples were also screened for HIV infection. A sero-prevalence of 59.0% was recorded for the study area. The sero-prevalence was higher among the gynaecologic patients (62.0%) than the pregnant women (57.5%). The difference was statistically significant (P = 0.0001). Of the four centers chosen for the study, the Federal Capital Territory (Abuja) had the highest prevalence (84.7%), while Niger State had the least (28.7%). The difference was also statistically significant (P<0.0001). The prevalence rate of HIV among the participants in the study center was found to be 17.2%. Abuja had the highest prevalence rate of 24.6%, followed by Benue, 16.7%, then Kogi, 12.0% and finally, Niger, 4.7%. Chlamydia was found to be associated with HIV (p<0.0001). The sero-prevalence of chlamydia in the North-Central zone of Nigeria was found to be high. Chlamydia was found to be correlated with HIV in the study area and may have contributed to the zone emerging with the highest HIV prevalence in the country. For an infection that is largely asymptomatic but has devastating effects on populations, only a preventive approach would have beneficial effects in controlling the disease and its effects on women’s health in the country.Keywords: Chlamydia trachomatis, HIV, pregnant women, gynaecologic patients, co-infectio

Assessment of Orange (Citrus Sinensis) Supply Chain Activities in Kano State

The study focused on the handling system of orange in Yanlemo Market of Kano State. A field study was conducted with structural questionnaires that targeted the orange supplies, traders and agricultural equipment suppliers/fabricators. Result obtained reveals that the orange handling activity is dominated by male traders. Average of 2 to 5 bags of oranges are usually handled by about 53.3% of the respondents, while 8.3% handle more than 10 bags of the oranges daily. Dan Tivi was found to be the commonest orange variety in the study area. Some other varieties established in the study area are; Dan Nassarawa, Dan Ondo and Dan Delta representing about 8.3, 1.7 and 1.7% of the varieties handled in the study area respectively. The mode of transportation, sorting, and washing was found to be manual with a lot of challenges. The predominant manual handling of the orange established in the study area could be amongst the major reasons for the high losses usually recorded by the traders on a daily basis. Thus, useful suggestions that could be employed by researchers and policymakers to provide improvements in the supply chain activities to prevent such losses are presented.  Keywords— Orange, Assessment, Supply Chain, Handlin

Effects of zinc oxide nanoparticles on viscosity of transesterified neem oil

Efforts have been made worldwide to find alternative fluids for industrial applications. Vegetable oil appears to be a perfect alternative, but using most of the vegetable oil as a feedstock made its use for industrial purposes challenging. The recent trend is to develop coolants/lubricants from non-edible seed oil. This work investigates the effects of zinc oxide nanoparticles on viscosity of transesterified neem oil. The crude neem oil was purified, transesterified and nanoparticles were dispersed in the transesterified oil at concentrations ranging from 0.0% to 1.0% at 0.2% intervals. Fourier Transform Infrared (FTIR) spectra were used to examine the structures of the samples and Scanning Electron Microscopy (SEM) analyses were used to examine the surface morphology of the samples. Viscosity were examined. Among other things, it was found that a small amount of ZnO (0.6%) nanoparticles in the oil could improve the viscosity of the fluid. The nanoliquid with a ZnO concentration of 0.6% appears to have optimal properties

Studies on Haematology and Serum Biochemistry of Broiler Chickens Finished on an Unprocessed and Processed Velvet Bean ( Mucuna Pruriens (L.)) as Dietary Protein Sources

The effects of different processed velvet seed on haematology and serum indices of Broiler chickens was studied using 150 four weeks old broiler chicks. The birds were randomly assigned to five treatment groups of 30 birds with three replications of 10 birds each. The chickens were finished on five dietary treatments formulated to contain 5% raw, soaked and boiled, cracked and boiled and roasted velvet bean seed meal as protein source in diets 1, 2, 3, 4 and 5 respectively. The haematological parameters indicated no significant difference (P> 0.05) among treatment groups for PCV, Hb, RBC, WBC, MCHC and PLT while MCV and MCH differ significantly (P< 0.05) where chickens on treatment T3 (boiled) had the highest values of 133.33 fl and 42.80 pg while lower values were chickens in T4 (cracked and boiled) 123.90 fl and 39.80 pg as the lowest. Urea concentration range of 7.88-10.08 mmol/l obtained with significant difference (P< 0.05) among treatment groups with T5(roasted) having value of 10.08 mmol/l and T2 (soaked) having least value of sodium 7.88 mmol/l. Serum electrolytes such as Sodium (Na+), Chloride (Cl-) and bicarbonate showed significant (P< 0.05) difference among treatment groups. The study concluded that, on account of adequate haematocrit and immune statuses, in addition to its hypoglycaemic ability, boiling mucuna seed meal with 5.00 % level of inclusion can be used without any deleterious effect on haematological and serum biochemical assay parameters of broiler chickens