Genomic prediction and quantitative trait locus discovery in a cassava training population constructed from multiple breeding stages

Open Access Article; Published online: 11 Dec 2019Assembly of a training population (TP) is an important component of effective genomic selection‐based breeding programs. In this study, we examined the power of diverse germplasm assembled from two cassava (Manihot esculenta Crantz) breeding programs in Tanzania at different breeding stages to predict traits and discover quantitative trait loci (QTL). This is the first genomic selection and genome‐wide association study (GWAS) on Tanzanian cassava data. We detected QTL associated with cassava mosaic disease (CMD) resistance on chromosomes 12 and 16; QTL conferring resistance to cassava brown streak disease (CBSD) on chromosomes 9 and 11; and QTL on chromosomes 2, 3, 8, and 10 associated with resistance to CBSD for root necrosis. We detected a QTL on chromosome 4 and two QTL on chromosome 12 conferring dual resistance to CMD and CBSD. The use of clones in the same stage to construct TPs provided higher trait prediction accuracy than TPs with a mixture of clones from multiple breeding stages. Moreover, clones in the early breeding stage provided more reliable trait prediction accuracy and are better candidates for constructing a TP. Although larger TP sizes have been associated with improved accuracy, in this study, adding clones from Kibaha to those from Ukiriguru and vice versa did not improve the prediction accuracy of either population. Including the Ugandan TP in either population did not improve trait prediction accuracy. This study applied genomic prediction to understand the implications of constructing TP from clones at different breeding stages pooled from different locations on trait accuracy

Community phytosanitation to manage cassava brown streak disease

Article purchased; Published online: 6 May 2017Cassava viruses are the major biotic constraint to cassava production in Africa. Community-wide action to manage them has not been attempted since a successful cassava mosaic disease control programme in the 1930s/40 s in Uganda. A pilot initiative to investigate the effectiveness of community phytosanitation for managing cassava brown streak disease (CBSD) was implemented from 2013 to 2016 in two communities in coastal (Mkuranga) and north-western (Chato) Tanzania. CBSD incidence in local varieties at the outset was >90%, which was typical of severely affected regions of Tanzania. Following sensitization and monitoring by locally-recruited taskforces, there was effective community-wide compliance with the initial requirement to replace local CBSD-infected material with newly-introduced disease-free planting material of improved varieties. The transition was also supported by the free provision of additional seed sources, including maize, sweet potato, beans and cowpeas. Progress of the initiative was followed in randomly-selected monitoring fields in each of the two locations. Community phytosanitation in both target areas produced an area-wide reduction in CBSD incidence, which was sustained over the duration of the programme. In Chato, maximum CBSD incidence was 39.1% in the third season, in comparison with an incidence of >60% after a single season in a control community where disease-free planting material was introduced in the absence of community phytosanitation. Kriging and geospatial analysis demonstrated that inoculum pressure, which was a function of vector abundance and the number of CBSD-infected plants surrounding monitored fields, was a strong determinant of the pattern of CBSD development in monitored fields. In the first year, farmers achieved yield increases with the new varieties relative to the local variety baseline of 94% in Chato (north-west) and 124% in Mkuranga (coast). Yield benefits of the new material were retained up to the final season in each location. The new variety (Mkombozi) introduced under community phytosanitation conditions in Chato yielded 86% more than the same variety from the same source planted in the no-phytosanitation control location. Although there was an 81% reduction in CBSD incidence in the new variety Kiroba introduced under community phytosanitation compared to control conditions in Mkuranga, there was no concomitant yield increase. Variety Kiroba is known to be tolerant to the effects of CBSD, and tuberous roots of infected plants are frequently asymptomatic. Community phytosanitation has the potential to deliver area-wide and sustained reductions in the incidence of CBSD, which also provide significant productivity gains for growers, particularly where introduced varieties do not have high levels of resistant/tolerance to CBSD. The approach should therefore be considered as a potential component for integrated cassava virus management programmes, particularly where new cassava plantations are being established in areas severely affected by CBSD

Rural schools as effective hubs for agricultural technology dissemination: experimental evidence from Tanzania and Uganda

Increasing agricultural productivity by promoting high-yielding and micronutrient-rich crop varieties has the potential to reduce poverty and malnutrition. However, getting these technologies into the hands of smallholders remains a challenge. This paper presents results from a randomised field experiment that uses rural primary schools as dissemination hubs for improved orange-fleshed sweet potato (OFSP) vines and nutrition information in rural Tanzania and Uganda. Two years after the initial vine distribution, we find that households in treatment villages are 21 percentage points more likely to report growing OFSP and 27 percentage points more likely to correctly state the nutritional benefits of OFSP compared to those in control villages. We also find up to 16 percentage point increase in the likelihood of OFSP consumption by children under 5 years of age in treatment villages compared to that in control villages. Furthermore, we find suggestive evidence that increased knowledge on the nutritional benefits of OFSP mediated up to a third of the total treatment effect on OFSP adoption and consumption. Our findings suggest that rural primary schools can be effective channels for promoting and accelerating the diffusion of micronutrient-rich crop varieties in rural areas

Assessing the degeneration of cassava under high-virus inoculum conditions in coastal Tanzania

Open Access Article; Published online: 19 Jul 2019Cassava brown streak disease (CBSD), caused by cassava brown streak ipomoviruses (CBSIs), has become the most debilitating biotic stress to cassava production in East and Central Africa. Lack of CBSD-resistant varieties has necessitated the search for alternative control measures. Most smallholder farmers reuse stems from previous crops for planting in the new season. Recycling planting material in this way can lead to “degeneration” owing to the compounding effects of disease. In this study, degeneration was defined as the increase in CBSD incidence and reduction in marketable root yield over time. An experiment was established to study the rates of degeneration in selected cassava varieties Chereko, KBH2002_135, Kipusa, Kizimbani, and Mkuranga1 and cultivars Kiroba and Kikombe under high-CBSD inoculum conditions in Bagamoyo, Tanzania from 2013 to 2017. The experiment was replicated across two seasons: the first planted during the long rains (Masika) between March and June and the second planted during the short rains (Vuli) between October and December. Mean abundance of the whitefly vector (Bemisia tabaci) was much greater during the Vuli season (>19 insects per plant) than the Masika season (<2 insects per plant). CBSD shoot symptoms occurred naturally and were observed only on Kikombe, Kiroba, and Kipusa. New materials had overall lower CBSD shoot incidences (1.5%) compared with recycled materials (6.9%) in Masika, although no significant differences were obvious in Vuli. However, Masika (8.7%) had an overall lower CBSD shoot incidence than Vuli (16.5%) in the varieties that had shoot symptoms. CBSD root incidences were higher in Vuli (10.3%) than Masika (4.4%), and root yields in Masika (29.4 t/ha) were significantly greater than those in Vuli (22.5 t/ha). The highest percentage of roots rendered unusable owing to CBSD was observed in Vuli. There was significantly higher unusable root incidence in recycled materials (3.7%) than in new materials (1.4%) in Masika but not in Vuli. Overall root yield was similar between recycled and new materials in either season. Significant reductions in root yield over the course of the experiment were observed both in Masika and Vuli, whereas changes in marketable yield were significant only in Masika. Differences in the response of varieties to degeneration led to the identification of four degeneration patterns, namely “strong,” “moderate,” “mild,” and “delayed” degeneration. The strongest effects of degeneration were most obvious in the susceptible cultivar (Kikombe), which also had the lowest marketable yield in either season. Seasonal differences were a key driver of degeneration, because its effects were much greater in Vuli than Masika. To the best of our knowledge, this work reports the first study of degeneration caused by cassava viruses

Effect of leaf harvesting frequency on growth and yield of sweetpotato in the Lake Zone of Tanzania

The objectives of this study were to assess the effects of leaf harvesting frequency on growth and yield of sweetpotato (Ipomoea batatus L.); to determine the suitable varietal types for use as source of leaf vegetable and to determine the suitable leaf harvesting frequency(ies) for reduced detrimental effects on growth and yield of sweetpotato. Two narrow leafed varieties Budagala and Mwananjemu and two broad leafed varieties SPN/O and Biganana were used in a randomised complete block design with 3 replications. Plot size was 8.4 m2 . Tip cuttings of 20 cm long were used as planting materials. The fully expanded top and tender leaves were plucked, counted and weighed. Tender leaves were harvested once, twice and three times during the growing period. Results showed that there were small differences between varieties and harvesting frequencies, however, harvesting at one month intervals for three times gave the highest leaf vegetable production although the yield was affected tremendously. Harvesting twice in a growing period proved the best in leaf production as well as root yields. Overall, there were significant differences between varieties and harvesting frequencies. Key Words: Ipomoea batatus, leaf harvesting, Tanzania, tuber and vine yields (African Crop Science Journal 2001 9(1): 97-103

Performance of cassava brown streak disease-tolerant varieties in Zanzibar, Tanzania

Cassava is an important staple food in subtropical regions; however, its production is adversely affected by cassava brown streak disease and poor soil fertility. Five improved and two local cassava varieties were evaluated for three seasons across two sites in Kizimbani, Zanzibar. Highly significant differences were detected among varieties, sites and years for fresh shoot yield, and fresh root yield. For cassava brown streak disease associated root necrosis, highly significant differences were detected only between varieties but not sites or years. On average, the site that had a slightly higher soil nitrogen level recorded ~126% higher fresh root yield. Two improved varieties, ‘Kizimbani’ and ‘Machui’, produced significantly higher fresh root yields than the best local variety, ‘Mwari’. However, the local variety ‘Boma’ is preferred by farmers in Zanzibar because it has better fresh consumption qualities than ‘Mwari’. ‘Boma’ is highly susceptible to cassava brown streak disease and produces a poor yield. The four released varieties, ‘Kama’, ‘Kizimbani’, ‘Mahonda’ and ‘Machui’ were superior to ‘Boma’ in cassava brown streak disease resistance and yield. Further, soil fertility improvement and production system intensification are needed to enhance productivity

Transmission of Cassava brown streak virus by Bemisia tabaci (Gennadius)

Two whitefly species, Bemisia afer (Priesner & Hosny) and B. tabaci (Gennadius) were used in transmission experiments with Cassava brown streak virus (CBSV) (Ipomovirus; Potyviridae). Adults of whiteflies were given access to CBSV by containing them in clip cages on CBSV-infected cassava plants. Whiteflies were then transferred, together or separately, to CBSD-susceptible cassava plants of var. 'Albert' in a controlled environment. In glasshouse experiments, whiteflies were caged with CBSV-infected and virus-free cassava plants. Transmission of CBSV was sporadic and occurred in three of seven experiments when inoculated by B. afer and B. tabaci or B. tabaci alone, but not by B. afer alone. However, rate of transmission was low (maximum 22%) even when using high whitefly numbers of up to 120 per target plant. Successful transmission was confirmed by the detection of CBSV by reverse transcription-polymerase chain reaction. Spread of cassava brown streak disease (CBSD) in the field in Tanzania coincided with increases in whitefly numbers; further supporting the evidence that B. tabaci is a vector of CBSV. The results of this study establish for the first time that B. tabaci is the vector of CBSV, similar to other ipomoviruses