Up-regulation of uPARAP/Endo180 during culture activation of rat hepatic stellate cells and its presence in hepatic stellate cell lines from different species

<p>Abstract</p> <p>Background</p> <p>The urokinase plasminogen activator receptor associated protein (uPARAP)/Endo180 is a novel endocytic receptor that mediates collagen uptake and is implicated to play a role in physiological and pathological tissue-remodelling processes by mediating intracellular collagen degradation.</p> <p>Result</p> <p>This study investigates the expression of uPARAP/Endo180 protein and messenger RNA in primary rat hepatic stellate cell (HSC) cultures. The results show that uPARAP/Endo180 protein is not expressed in freshly isolated HSCs or during the first few days of culture while the cells still display quiescent features. In contrast, uPARAP/Endo180 protein is expressed early during HSC activation when cells are transdifferentiated into myofibroblast-like cells. Very low levels of uPARAP/Endo180 mRNA are detectable during the first days of culture but uPARAP/Endo180 mRNA is strongly up-regulated with increasing time in culture. Moreover, endocytic uptake of denatured collagen increases as transdifferentiation proceeds over time and correlates with increased expression of uPARAP/Endo180. Finally, analysis of uPARAP/Endo180 expression in four hepatic stellate cell lines from three different species showed that all these cell lines express uPARAP/Endo180 and are able to take up denatured collagen efficiently.</p> <p>Conclusion</p> <p>These results demonstrate that uPARAP/Endo180 expression by rat HSCs is strongly up-regulated during culture activation and identify this receptor as a feature common to culture-activated HSCs.</p

Improved genetic algorithms by means of fuzzy crossover operators for revenue management in airlines

Abstract: Revenue Management is an economic policy that increases the earned profit by adjusting the service demand and inventory. Revenue Management in airlines correlates with inventory control and price levels in different fare classes. We focus on pricing and seat allocation problems in airlines by introducing a constrained optimization problem in Binary Integer Programming (BIP) formulation. Two BIP problems are represented. Moreover, some improved Genetic Algorithms (GAs) approaches are used to solve these problems. We introduce new crossover operators that assign a Fuzzy Membership Function to each parent in GAs. We achieve better outputs with new methods that take lower calculation times and earn higher profits. Three different test problems in different scales are selected to evaluate the effectiveness of each algorithm. This paper defines new crossover operators that help to reach better solutions that take lower calculation times and more earned profits

A class of semihypergroups connected to preordered weak Γ-semigroups

AbstractWe introduce the concept of weak Γ-semigroups as a generalization of Γ-semigroups. Using preordered weak Γ-semigroups, we obtain a class of semihypergroups and we analyze them in this paper. A connection between morphisms of semihypergroups associated with preordered Γ-semigroups and morphisms of preordered structures is also investigated

A QUICK SEASONAL DETECTION AND ASSESSMENT OF INTERNATIONAL SHADEGAN WETLAND WATER BODY EXTENT USING GOOGLE EARTH ENGINE CLOUD PLATFORM

Understanding the variation of Water Extent (WE) can provide insights into Wetland conservation and management. In this study, and-inter inner-annual variations of WE were analyzed during 2019–2021 to understand the spatiotemporal changes of the International Shadegan Wetland, Iran. We utilized a thresholding process on Modified Normalized Difference Water Index (MNDWI) to extract the WE quickly and accurately using the Google Earth Engine (GEE) platform. The water surface analysis showed that: (1) WE had a downward trend from 2019 to 2021, with the overall average WE being 1405.23&thinsp;km2; (2) the water area reached its peak due to the water supply to International Shadegan Wetland through the Jarahi River and upstream reservoirs at the end of 2019 and the beginning of 2020, and the largest water body appeared in Winter 2019, reaching 1953.31&thinsp;km2. In contrast, the smallest water body appeared in Autumn 2021, reaching 563.56&thinsp;km2; (3) The WE of the wetland showed predictable seasonal characteristics. The water area in Winter was the largest, with an average value of 1829.1&thinsp;km2, while it was the smallest in Summer, with an average value of 1100.3&thinsp;km2; (4) The average water area in 2019 was 1490.5&thinsp;km2 whereas in 2020 and 2021 decreased by 9% and 25%, respectively, and reached 968.6&thinsp;km2 and 811.9&thinsp;km2. Finally, to evaluate the proposed model, its results were compared with the Random Forest (RF) classification results. Accordingly, Histogram Analysis (HA) classification achieved 94.6% of the average overall accuracy and the average Kappa coefficient of 0.93, but the RF method obtained 95.38% of the average overall accuracy and an average Kappa coefficient of 0.94

The burden of rheumatoid arthritis in the Middle East and North Africa region, 1990–2019

Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease. The present study reported the burden of RA in the Middle East and North Africa (MENA) region from 1990 to 2019 by age, sex, and socio-demographic index (SDI). Publicly available data from the Global Burden of Disease (GBD) 2019 study was used to report the modelled point prevalence, annual incidence, and disability-adjusted life-years (DALYs) of RA, as counts and age-standardised rates with their corresponding 95% uncertainty intervals (UIs). In 2019, RA had an age-standardised point prevalence of 120.6 per 100,000 population (107.0–135.7) and an annual incidence rate of 5.9 (5.2–6.6) in MENA, which have increased 28.3% and 25.2%, respectively, since 1990. In 2019, the number of DALYs due to RA in the region was 103.6 thousand (74.2–136.7), with an age-standardised rate of 19.0 (13.9–24.9) DALYs per 100,000 population, which has increased by 18.6% since 1990 (6.7–28.2). The highest point prevalence was found in females aged 50–54, and in males aged 45–49. The highest number of DALYs was observed in the 50–54 age group. The MENA DALY rate was lower than the global rate (19.0 vs. 39.6 per 100,000), but the rate was higher in all age groups in 2019, when compared with 1990. In addition, from 1990 to 2019 an increased burden from RA was associated with an increase in SDI. In line with global trends, the burden of RA in the MENA region showed a steady increase from 1990 to 2019. This highlights the increasing need for updating the available health data to design more accurate guidelines to enable the early detection and treatment of RA in the MENA countries

Biodiesel fuel purification in a continuous centrifugal contactor separator:An environmental-friendly approach

Wet washing is the most common method for biodiesel purification, but higher water consumption, longer purification time, and high expenses bring limitations on the use of this process. One of the suitable methods to remove such limitations is to use new techniques for purification. In this study, biodiesel purification was evaluated in a novel continuous centrifugal contactor separator (CCCS) at 0.5:1, 1:1, and 1.5:1 (V/V%)water to biodiesel ratio; 10 Hz, 20 Hz, and 30 Hz device working frequency; and 25 °C, 40 °C, and 55 °C temperatures. A mathematical model for the wet-washing process of biodiesel and energy consumption of the CCCS device using the response surface method is proposed. A 0.8:1 (V/V%) water to biodiesel ratio, 10 Hz working frequency, and 35 °C temperature were found to be the optimal conditions in the experiment. At this point, the biodiesel yield and the amount of energy consumption were reported to be 96% and 17 kJ, respectively. The results showed that compared to the traditional wet washing method, the biodiesel purification method using CCCS is cost-effective and consumes more than 75% less of water

Kidney cancer in the Middle East and North Africa region: a 30-year analysis (1990–2019)

Kidney cancer, a type of urogenital cancer, imposes a high burden on patients. Despite this, no recent research has evaluated the burden of this type of cancer in the Middle East and North Africa (MENA) region. This study explored the burden of kidney cancer from 1990 to 2019 according to age, sex and socio-demographic index (SDI). The Global Burden of Disease (GBD) 2019 data was utilized to estimate the incidence, death, and disability-adjusted life-years (DALYs) caused by kidney cancer. These estimates were reported as counts and as age-standardised rates with 95% uncertainty intervals (UIs). The estimated age-standardised incidence, mortality, and DALY rates of kidney cancer in 2019 were 3.2 (2.8–3.6), 1.4 (1.2–1.6), and 37.2 (32.0–42.6) per 100,000, respectively. Over the period from 1990 to 2019, these rates have increased by 98.0%, 48.9%, and 37.7%, respectively. In 2019, the United Arab Emirates, Qatar, and Lebanon had the largest age-standardised incidence, mortality, and DALY rates. The smallest age-standardised incidence rates were seen in Yemen, Afghanistan, and the Syrian Arab Republic. Additionally, the smallest age-standardised mortality and DALY rates were observed in the Syrian Arab Republic, Yemen, and Morocco. The highest incidence rates were found among individuals aged 75–79 in both males and females. In 2019, the MENA/Global DALY ratio exceeded one for females aged 5–19 age and males aged 5–14, compared to 1990age groups in males. The burden of kidney cancer consistently rose with increasing SDI levels from 1990 to 2019. The increasing burden of kidney cancer highlights the urgent need for interventions aimed at improving early diagnosis and treatment in the region

Antiphospholipid antibodies: Paradigm in transition

OBJECTIVES: This is a critical review of anti-phospholipid antibodies (aPL). Most prior reviews focus on the aPL syndrome (APS), a thrombotic condition often marked by neurological disturbance. We bring to attention recent evidence that aPL may be equally relevant to non-thrombotic autoimmune conditions, notably, multiple sclerosis and ITP. ORGANIZATION: After a brief history, the recent proliferation of aPL target antigens is reviewed. The implication is that many more exist. Theories of aPL in thrombosis are then reviewed, concluding that all have merit but that aPL may have more diverse pathological consequences than now recognized. Next, conflicting results are explained by methodological differences. The lupus anticoagulant (LA) is then discussed. LA is the best predictor of thrombosis, but why this is true is not settled. Finally, aPL in non-thrombotic disorders is reviewed. CONCLUSION: The current paradigm of aPL holds that they are important in thrombosis, but they may have much wider clinical significance, possibly of special interest in neurology

Higher Adherence to the Mediterranean Dietary Pattern Is Inversely Associated With Severity of COVID-19 and Related Symptoms: A Cross-Sectional Study

Background and AimsAdherence to the Mediterranean diet (MD) has been associated with a decreased risk of developing a variety of chronic diseases that are comorbidities in COVID-19 patients. However, its association to the severity and symptoms of COVID-19 are still unknown. This study aimed to examine the association between adherence to the MD pattern and COVID-19 severity and symptoms in Iranian hospitalized patients.MethodsIn this cross-sectional study, 250 COVID-19 patients aged 18 to 65 were examined. We employed a food frequency questionnaire (FFQ) to obtain data on dietary intake of participants in the year prior to their COVID-19 diagnosis. COVID-19 severity was determined using the National Institutes of Health's Coronavirus Disease 2019 report. Additionally, symptoms associated with COVID-19, inflammatory markers, and other variables were evaluated. The scoring method proposed by Trichopoulou et al. was used to assess adherence to the MD.ResultsThe participants' mean age was 44.1 ± 12.1 years, and 46% of them had severe COVID-19. Patients who adhered more closely to the MD had lower serum C-reactive protein levels (7.80 vs. 37.36 mg/l) and erythrocyte sedimentation rate (14.08 vs. 42.65 mm/h). Those with the highest MD score were 77% less likely to have severe COVID-19 after controlling for confounding variables. The MD score was also found to be inversely associated with COVID-19 symptoms, including dyspnea, cough, fever, chills, weakness, myalgia, nausea and vomiting, and sore throat.ConclusionHigher adherence to the MD was associated with a decreased likelihood of COVID-19 severity and symptoms, as well as a shorter duration of hospitalization and convalescence, and inflammatory biomarkers