The Anti-staphylococcus Aureus Effect Of Combined Echinophora Platyloba Essential Oil And Liquid Smoke In Beef

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)In the current study, the antibacterial effect of Echinophora platyloba essential oil and common liquid smoke (individually and in combination) against Staphylococcus aureus in beef meat samples is investigated. Using an automated microbiological growth analyser and the turbidimetric technique, the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) of the essential oil and liquid smoke were determined. Anti-S. aureus activity of essential oil and liquid smoke (individually and in combination) was defined by disk diffusion assay, generation time and cell constituent release. Apart from that, the interactions between these two compounds were measured by the checkerboard assay and by calculating the fractional inhibitory concentration (FIC) indices. Related MIC values of essential oil and smoke were found to be 7200 and 5500 mg/L, and MBC values were 8500 and 8000 mg/L, respectively. The conducted organoleptic assay showed that the addition of 0.05 g of essential oil and 0.6 g of liquid smoke to 100 g of meat samples did not have adverse effect on the overall acceptance. Weaker antibacterial effect against Staphylococcus aureus was observed when only Echinophora platyloba essential oil was used than when it was used in combination with liquid smoke.551117124Student Research Committee, Shahid Beheshti University of Medical Sciences [1395-77359]CNPq-TWAS [324027 4290]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

H(N3)dap (Hdap = 2,6-Diaminopurine) Recognition by Cu2(EGTA): Structure, Physical Properties, and Density Functional Theory Calculations of [Cu4( -EGTA)2( -H(N3)dap)2(H2O)2] 7H2O

Reactions inwater between the Cu2( -EGTA) chelate (EGTA= ethylene-bis(oxyethyleneimino) tetraacetate(4-) ion) and Hdap in molar ratios 1:1 and 1:2 yield only blue crystals of the ternary compound [Cu4( -EGTA)2( -H(N3)dap)2(H2O)2] 7H2O (1), which has been studied via single-crystal X-ray diffraction and various physical methods (thermal stability, spectral and magnetic properties), as well as DFT theoretical calculations. In the crystal, uncoordinated water is disordered. The tetranuclear complex molecule also has some irrelevant disorder in an EGTA-ethylene moiety. In the complex molecule, both bridging organic molecules act as binucleating ligands. There are two distorted five- and two six-coordinated Cu(II) centers. Each half of EGTA acts as a tripodal tetradentate Cu(II) chelator, with a mer-NO2 + O(ether, distal) conformation. Hdap exhibits the tautomer H(N3)dap, with the dissociable H-atom on its less basic N-heterocyclic atom. These features favor the efficient cooperation between Cu-N7 or Cu-N9 bonds with appropriate O-EGTA atoms, as N6-H O or N3-H O interligand interactions, respectively. The bridging role of both organics determines the tetranuclear dimensionality of the complex. In this crystal, such molecules associate in zig-zag chains built by alternating – interactions between the five- or six-atom rings of Hdap ligands of adjacent molecules. DFT theoretical calculations (using two different theoretical models and characterized by the quantum theory of “atoms in molecules”) reveal the importance of these – interactions between Hdap ligands, as well as those corresponding to the referred hydrogen bonds in the contributed tetranuclear moleculeMICIU/AEI of Spain project PID2020-115637GB-I00 FEDER, MICINN of Spain project PGC2018-102047-B-I00Project B-FQM-478-UGR20 (FEDER-Universidad de Granada, Spain)FQM-283 (Junta de Andalucía, Spain

Utjecaj esencijalnog ulja biljke Echinophora platyloba i primarnog kondenzata dima na suzbijanje rasta bakterije Staphylococcus aureus u goveđem mesu

In the current study, the antibacterial effect of Echinophora platyloba essential oil and common liquid smoke (individually and in combination) against Staphylococcus aureus in beef meat samples is investigated. Using an automated microbiological growth analyser and the turbidimetric technique, the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) of the essential oil and liquid smoke were determined. Anti-S. aureus activity of essential oil and liquid smoke (individually and in combination) was defined by disk diffusion assay, generation time and cell constituent release. Apart from that, the interactions between these two compounds were measured by the checkerboard assay and by calculating the fractional inhibitory concentration (FIC) indices. Related MIC values of essential oil and smoke were found to be 7200 and 5500 mg/L, and MBC values were 8500 and 8000 mg/L, respectively. The conducted organoleptic assay showed that the addition of 0.05 g of essential oil and 0.6 g of liquid smoke to 100 g of meat samples did not have adverse effect on the overall acceptance. Weaker antibacterial effect against Staphylococcus aureus was observed when only Echinophora platyloba essential oil was used than when it was used in combination with liquid smoke.U radu je ispitan pojedinačni i zbirni učinak esencijalnog ulja biljke Echinophora platyloba i primarnog kondenzata dima na suzbijanje rasta bakterije Staphylococcus aureus u uzorcima goveđeg mesa. Pomoću automatskog sustava za detekciju mikrobiološkog rasta i turbidimetrije određene su minimalna inhibicijska koncentracija i minimalna baktericidna koncentracija esencijalnog ulja i primarnog kondenzata dima. Pojedinačni i zbirni antibakterijski učinak esencijalnog ulja i primarnog kondenzata dima ispitani su disk difuzijskom metodom, određivanjem generacijskog vremena i mjerenjem apsorbancije otpuštenog staničnog sadržaja bakterije Staphylococcus aureus. Osim toga, ispitana je interakcija između ta dva antimikrobna agensa mikrorazrjeđenjem u bujonu (tzv. checkerboard testom) i određivanjem indeksa interakcije. Minimalna inhibicijska koncentracija esencijalnog ulja bila je 7200 mg/L, a primarnog kondenzata dima 5500 mg/L, dok je minimalna baktericidna koncentracija esencijalnog ulja bila 8500 mg/L, a primarnog kondenzata dima 8000 mg/L. Ispitana su organoleptička svojstva uzoraka mesa i utvrđeno je da dodatak 0,05 g esencijalnog ulja i 0,6 g kondenzata dima u 100 g uzorka nije utjecao na prihvatljivost proizvoda. Esencijalno ulje biljke Echinophora platyloba imalo je slabiji učinak na suzbijanje rasta bakterije Staphylococcus aureus od kombinacije esencijalnog ulja i primarnog kondenzata dima

Anti-Fatigue Effect of Viola odorata L. in Forced Swimming Test in Rat

Fatigue is a complex phenomenon that is explained as difficulty starting or keeping voluntary physical or mental activity leading to negative impacts on life and work performance. This study aimed to investigate the anti-fatigue effects of Viola odorata L. in an animal model. Aqueous and ethanol extracts of V. odorata were prepared and total phenolics content was determined. Then, the anti-fatigue activity of the extracts was evaluated via a weight-loaded forced swimming test in the rat. To this end, 48 male Wistar rats were randomly classified into 6 groups. The control group using distilled water and other groups with ethanol (EVO; 50, 100, 200 mg/kg) and aqueous extracts of V. odorata (WVO; 50, 100mg/kg) were gavaged once daily for four weeks. Then, the forced swimming was conducted and swimming time, as a fatigue factor was measured. In addition, to validate the effect of V. odorata on the endurance capacity of the rats, biochemical factors including glucose (Glc), lactate dehydrogenase (LDH), and tumor necrosis factor (TNF-α) were examined in the serum. Hepatotoxicity was also assessed using hematoxylin-eosin staining. Our Data indicated that the forced swimming time of the EVO-100, EVO-200, and WVO-100 groups was significantly increased. The serum Glucose in the group which received EVO-200 was increased significantly, while serum LDH levels in all treated groups were significantly decreased. Also, the serum level of TNF-α in the groups which received EVO-100 or 200 was increased significantly. However, there was no considerable difference in serum TNF-α level and no hepatotoxicity within aqueous extract groups. Pathology results showed fewer effects of the aqueous extract rather than ethanol extract on the liver. The results provide evidence for the development and use of V. odorata aqueous extract as an anti-fatigue supplement

Comparative proteomic profiling of Leishmania tropica: investigation of a case infected with simultaneous cutaneous and viscerotropic leishmaniasis by 2-dimentional electrophoresis and mass spectrometry

Background: Viscerotropic leishmaniasis caused by Leishmania tropica poses a significant prob­lem in the diagnosis and treatment management. Since differential gene expression is more im­portant in outcome of the infection, we employed proteomic approach to identify potential pro­teins involved in visceralization of L. tropica. Methods: The proteomes profiling of L. tropica isolated from cutaneous and visceral tissues of one host were compared by 2-DE/MS proteomics study. Moreover, the transcript level of some identified proteins was confirmed using real-time RT-PCR. Results: Of the 700 protein spots that were detected reproducibly on each gel, 135 were found to be differentially expressed (P≤ 0.05). Most of responsive proteins in visceral isolate changed in less abundant compared to cutaneous isolate. Among differentially expressed proteins, 56 proteins were confidently identified and classified according to the biological process. The larg­est groups consist of proteins involved in carbohydrate metabolism and protein synthesis. Most of the identified proteins, which implicated in energy metabolism, cell signaling and virulence were down-regulated, whereas some proteins that have a role in protein folding, antioxidant defense and proteolysis were up-regulated in visceral form. Moreover, the transcript level of some identified proteins such as co-chaperon was confirmed using real-time RT-PCR. Conclusion: L. tropica probably uses different mechanisms for survival and multiplication in viscera to establish viscerotropic leishmaniasis. The current study provides some clues into the mechanisms underlying the dissemination of L. tropica

Comparative proteomic profiling of Leishmania tropica: investigation of a case infected with simultaneous cutaneous and viscerotropic leishmaniasis by 2-dimentional electrophoresis and mass spectrometry

Background: Viscerotropic leishmaniasis caused by Leishmania tropica poses a significant prob­lem in the diagnosis and treatment management. Since differential gene expression is more im­portant in outcome of the infection, we employed proteomic approach to identify potential pro­teins involved in visceralization of L. tropica. Methods: The proteomes profiling of L. tropica isolated from cutaneous and visceral tissues of one host were compared by 2-DE/MS proteomics study. Moreover, the transcript level of some identified proteins was confirmed using real-time RT-PCR. Results: Of the 700 protein spots that were detected reproducibly on each gel, 135 were found to be differentially expressed (P≤ 0.05). Most of responsive proteins in visceral isolate changed in less abundant compared to cutaneous isolate. Among differentially expressed proteins, 56 proteins were confidently identified and classified according to the biological process. The larg­est groups consist of proteins involved in carbohydrate metabolism and protein synthesis. Most of the identified proteins, which implicated in energy metabolism, cell signaling and virulence were down-regulated, whereas some proteins that have a role in protein folding, antioxidant defense and proteolysis were up-regulated in visceral form. Moreover, the transcript level of some identified proteins such as co-chaperon was confirmed using real-time RT-PCR. Conclusion: L. tropica probably uses different mechanisms for survival and multiplication in viscera to establish viscerotropic leishmaniasis. The current study provides some clues into the mechanisms underlying the dissemination of L. tropica

Simulation and Calculation of Magnetic and Thermal Fields of Human using Numerical Method and Robust Soft wares

A numerical calculation and simulation of the bio-heat transfer and magnetic flux equations has been presented in this article. It expresses heat and magnetic distribution and transfer phenomena within the human body.Magnetic simulation on the body using a predefined electrical and magnetically models has been performed. A 40-cylindersegment model was used as the geometry of the human body. Thermal infrared (IR) images were used to verifications of claims.Comparisons of IR images with their simulated and calculated models indicate that this method is effective in eliminating the influence of the thermal environmental and magnetic conditions. However, the difference between the images and the computerized results varies among segments.Our computer simulation can predict the skin and tissue temperature and magnetic fields distribution; it is valuable in that a user can employ it to various change parameters reflecting environmental and physiological conditions.Electrical- Medica

The Anti-Staphylococcus aureus Effect of Combined Echinophora platyloba Essential Oil and Liquid Smoke in Beef

In the current study, the antibacterial effect of Echinophora platyloba essential oil and common liquid smoke (individually and in combination) against Staphylococcus aureus in beef meat samples is investigated. Using an automated microbiological growth analyser and the turbidimetric technique, the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) of the essential oil and liquid smoke were determined. Anti-S. aureus activity of essential oil and liquid smoke (individually and in combination) was defined by disk diffusion assay, generation time and cell constituent release. Apart from that, the interactions between these two compounds were measured by the checkerboard assay and by calculating the fractional inhibitory concentration (FIC) indices. Related MIC values of essential oil and smoke were found to be 7200 and 5500 mg/L, and MBC values were 8500 and 8000 mg/L, respectively. The conducted organoleptic assay showed that the addition of 0.05 g of essential oil and 0.6 g of liquid smoke to 100 g of meat samples did not have adverse effect on the overall acceptance. Weaker antibacterial effect against Staphylococcus aureus was observed when only Echinophora platyloba essential oil was used than when it was used in combination with liquid smoke

The anti-staphylococcus aureus effect of combined echinophora platyloba essential oil and liquid smoke in beef

In the current study, the antibacterial effect of Echinophora platyloba essential oil and common liquid smoke (individually and in combination) against Staphylococcus aureus in beef meat samples is investigated. Using an automated microbiological growth analyser and the turbidimetric technique, the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) of the essential oil and liquid smoke were determined. Anti-S. aureus activity of essential oil and liquid smoke (individually and in combination) was defined by disk diffusion assay, generation time and cell constituent release. Apart from that, the interactions between these two compounds were measured by the checkerboard assay and by calculating the fractional inhibitory concentration (FIC) indices. Related MIC values of essential oil and smoke were found to be 7200 and 5500 mg/L, and MBC values were 8500 and 8000 mg/L, respectively. The conducted organoleptic assay showed that the addition of 0.05 g of essential oil and 0.6 g of liquid smoke to 100 g of meat samples did not have adverse effect on the overall acceptance. Weaker antibacterial effect against Staphylococcus aureus was observed when only Echinophora platyloba essential oil was used than when it was used in combination with liquid smoke551117124CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ324027 429