3,934 research outputs found
Techniques for using diazo materials in remote sensor data analysis
The use of data derived from LANDSAT is facilitated when special products or computer enhanced images can be analyzed. However, the facilities required to produce and analyze such products prevent many users from taking full advantages of the LANDSAT data. A simple, low-cost method is presented by which users can make their own specially enhanced composite images from the four band black and white LANDSAT images by using the diazo process. The diazo process is described and a detailed procedure for making various color composites, such as color infrared, false natural color, and false color, is provided. The advantages and limitations of the diazo process are discussed. A brief discussion interpretation of diazo composites for land use mapping with some typical examples is included
Performance, emissions, and physical characteristics of a rotating combustion aircraft engine
The RC2-75, a liquid cooled two chamber rotary combustion engine (Wankel type), designed for aircraft use, was tested and representative baseline (212 KW, 285 BHP) performance and emissions characteristics established. The testing included running fuel/air mixture control curves and varied ignition timing to permit selection of desirable and practical settings for running wide open throttle curves, propeller load curves, variable manifold pressure curves covering cruise conditions, and EPA cycle operating points. Performance and emissions data were recorded for all of the points run. In addition to the test data, information required to characterize the engine and evaluate its performance in aircraft use is provided over a range from one half to twice its present power. The exhaust emissions results are compared to the 1980 EPA requirements. Standard day take-off brake specific fuel consumption is 356 g/KW-HR (.585 lb/BHP-HR) for the configuration tested
Searching edges in the overlap of two plane graphs
Consider a pair of plane straight-line graphs, whose edges are colored red
and blue, respectively, and let n be the total complexity of both graphs. We
present a O(n log n)-time O(n)-space technique to preprocess such pair of
graphs, that enables efficient searches among the red-blue intersections along
edges of one of the graphs. Our technique has a number of applications to
geometric problems. This includes: (1) a solution to the batched red-blue
search problem [Dehne et al. 2006] in O(n log n) queries to the oracle; (2) an
algorithm to compute the maximum vertical distance between a pair of 3D
polyhedral terrains one of which is convex in O(n log n) time, where n is the
total complexity of both terrains; (3) an algorithm to construct the Hausdorff
Voronoi diagram of a family of point clusters in the plane in O((n+m) log^3 n)
time and O(n+m) space, where n is the total number of points in all clusters
and m is the number of crossings between all clusters; (4) an algorithm to
construct the farthest-color Voronoi diagram of the corners of n axis-aligned
rectangles in O(n log^2 n) time; (5) an algorithm to solve the stabbing circle
problem for n parallel line segments in the plane in optimal O(n log n) time.
All these results are new or improve on the best known algorithms.Comment: 22 pages, 6 figure
Using computer graphics to design Space Station Freedom viewing
Viewing requirements were identified early in the Space Station Freedom program for both direct viewing via windows and indirect viewing via cameras and closed-circuit television (CCTV). These requirements reside in NASA Program Definition and Requirements Document (PDRD), Section 3: Space Station Systems Requirements. Currently, analyses are addressing the feasibility of direct and indirect viewing. The goal of these analyses is to determine the optimum locations for the windows, cameras, and CCTV's in order to meet established requirements, to adequately support space station assembly, and to operate on-board equipment. PLAID, a three-dimensional computer graphics program developed at NASA JSC, was selected for use as the major tool in these analyses. PLAID provides the capability to simulate the assembly of the station as well as to examine operations as the station evolves. This program has been used successfully as a tool to analyze general viewing conditions for many Space Shuttle elements and can be used for virtually all Space Station components. Additionally, PLAID provides the ability to integrate an anthropometric scale-modeled human (representing a crew member) with interior and exterior architecture
Combined Docking and Quantum Chemical Study on CYP-mediated Metabolism of Estrogens in Man
Long-term exposure
to estrogens seriously increases the incidence
of various diseases including breast cancer. Experimental studies
indicate that cytochrome P450 (CYP) enzymes catalyze the bioactivation
of estrogens to catechols, which can exert their harmful effects via
various routes. It has been shown that the 4-hydroxylation pathway
of estrogens is the most malign, while 2-hydroxylation is considered
a benign pathway. It is also known experimentally that with increasing
unsaturation of ring B of estrogens the prevalence of the 4-hydroxylation
pathway significantly increases. In this study, we used a combination
of structural analysis, docking, and quantum chemical calculations
at the B3LYP/6-311+G* level to investigate the factors that influence
the regioselectivity of estrogen metabolism in man. We studied the
structure of human estrogen metabolizing enzymes (CYP1A1, CYP1A2,
CYP1B1, and CYP3A4) in complex with estrone using docking and investigated
the susceptibility of estrone, equilin, and equilenin (which only
differ in the unsaturation of ring B) to undergo 2- and 4-hydroxylation
using several models of CYP enzymes (Compound I, methoxy, and phenoxy
radical). We found that even the simplest models could account for
the experimental difference between the 2- and 4- hydroxylation pathways
and thus might be used for fast screening purposes. We also show that
reactivity indices, specifically in this case the radical and nucleophilic
condensed Fukui functions, also correctly predict the likeliness of
estrogen derivatives to undergo 2- or 4-hydroxylation
Evaluation of GEOS-5 Sulfur Dioxide Simulations During the Frostburg, MD 2010 Field Campaign.
Sulfur dioxide (SO2) is a major atmospheric pollutant with a strong anthropogenic component mostly produced by the combustion of fossil fuel and other industrial activities. As a precursor of sulfate aerosols that affect climate, air quality, and human health, this gas needs to be monitored on a global scale. Global climate and chemistry models including aerosol processes along with their radiative effects are important tools for climate and air quality research. Validation of these models against in-situ and satellite measurements is essential to ascertain the credibility of these models and to guide model improvements. In this study the Goddard Chemistry, Aerosol, Radiation, and Transport (GOCART) module running on-line inside the Goddard Earth Observing System version 5 (GEOS-5) model is used to simulate aerosol and SO2 concentrations. Data taken in November 2010 over Frostburg, Maryland during an SO2 field campaign involving ground instrumentation and aircraft are used to evaluate GEOS-5 simulated SO2 concentrations. Preliminary data analysis indicated the model overestimated surface SO2 concentration, which motivated the examination of mixing processes in the model and the specification of SO2 anthropogenic emission rates. As a result of this analysis, a revision of anthropogenic emission inventories in GEOS-5 was implemented, and the vertical placement of SO2 sources was updated. Results show that these revisions improve the model agreement with observations locally and in regions outside the area of this field campaign. In particular, we use the ground-based measurements collected by the United States Environmental Protection Agency (US EPA) for the year 2010 to evaluate the revised model simulations over North America
Characterisation of the Mouse Vasoactive Intestinal Peptide Receptor Type 2 Gene, Vipr2, and Identification of a Polymorphic LINE-1-like Sequence That Confers Altered Promoter Activity
The VPAC(2) receptor is a seven transmembrane spanning G protein-coupled receptor for two neuropeptides, vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP). It has a distinct tissue-specific, developmental and inducible expression that underlies an important neuroendocrine role. Here, we report the characterisation of the gene that encodes the mouse VPAC(2) receptor (Vipr2), localisation of the transcriptional start site and functional analysis of the promoter region. The Vipr2 gene contains 12 introns within its protein-coding region and spans 68.6 kb. Comparison of the 5′ untranslated region sequences for cloned 5′-RACE products amplified from different tissues showed they all were contained within the same exon, with the longest extending 111 bp upstream of the ATG start site. Functional analysis of the 3.2-kb 5′-flanking region using sequentially deleted sequences cloned into a luciferase gene reporter vector revealed that this region is active as a promoter in mouse AtT20 D16:16 and rat GH4C1 cell lines. The core promoter is located within a 180-bp GC-rich region proximal to the ATG start codon and contains potential binding sites for Sp1 and AP2, but no TATA-box. Further upstream, in two out of three mice strains examined, we have discovered a 496-bp polymorphic DNA sequence that bears a significant identity to mouse LINE-1 DNA. Comparison of the promoter activity between luciferase reporter gene constructs derived from the BALB/c (which contains this sequence) and C57BL/6J (which lacks this sequence) Vipr2 promoter regions has shown three-fold difference in luciferase gene activity when expressed in mouse AtT20 D16:16 and αT3-1 cells, but not when expressed in the rat GH4C1 cells or in COS 7 cells. Our results suggest that the mouse Vipr2 gene may be differentially active in different mouse strains, depending on the presence of this LINE-1-like sequence in the promoter region
Selected Toll-like Receptor Ligands and Viruses Promote Helper-Independent Cytotoxic T Cell Priming by Upregulating CD40L on Dendritic Cells
SummaryCD40L (CD154) on CD4+ T cells has been shown to license dendritic cells (DCs) via CD40 to prime cytotoxic T lymphocyte (CTL) responses. We found that the converse (CD40L on DCs) was also important. Anti-CD40L treatment decreased endogenous CTL responses to both ovalbumin and influenza infection even in the absence of CD4+ T cells. DCs expressed CD40L upon stimulation with agonists to Toll-like receptor 3 (TLR3) and TLR9. Moreover, influenza infection, which stimulates CTLs without help, upregulated CD40L on DCs, but herpes simplex infection, which elicits CTLs through help, did not. CD40L-deficient (Cd40lg−/−) DCs are suboptimal both in vivo in bone marrow chimera experiments and in vitro in mixed lymphocyte reactions. In contrast, Cd40lg−/− CD8+ T cells killed as effectively as wild-type cells. Thus, CD40L upregulation on DCs promoted optimal priming of CD8+ T cells without CD4+ T cells, providing a mechanism by which pathogens may elicit helper-independent CTL immunity
Review of recent issues on humor field
The paper presents a brief review of some original issues in English, gained by the main argument-the theme of humor from the standpoint of different scientific approaches, and from the perspective of various aspects of the consideration of humor. Humor occupies a significant place in the spiritual life of social communities and all spheres of society are permeated by this phenomenon. The papers of both established scholars and novice ones from different countries are summarized. An attempt is made to differentiate the works according to the research areas and to present them in the light of general research lines and further perspectives in the study of humor
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