Strategies to mitigate the emission of methane in pastures: Enteric methane: A review

The global population reached 7.9 billion in 2021, which represents a 160% increase in the number of people to be fed since 1960. Agricultural systems must sustainably meet food demand for this growing population while minimizing or mitigating potential environmental impacts, which are of growing concern to both consumers and the scientific community. High protein animal products (meat and milk) play a crucial part in human nutrition and pastures represent ~20% of the planet’s surface. Pastoral areas have a great influence on both ecological balance and human subsistence. Ruminant livestock production systems are hotly debated because of the emission of methane, which is produced during enteric fermentation of ingested food within the rumen. Methanogenesis is a naturally occurring process in the digestive system of ruminant animals and ingesting a high-quality diet has been shown to reduce methane production. An additional function of pastoral grasslands is the capacity of the soils to operate as carbon sinks. Well managed pastures absorb carbon from the atmosphere where it can add to soil organic matter directly, through residue decomposition or excrement returns. However, in Brazil and globally, the efficiency of animal productivity tends to be lower in extensively grazed farming systems. Changes to pasture and grazing management in combination with the adoption of technology is necessary to improve the quality of pastures, increase animal productivity, and consequently reduce methane emissions from ruminant livestock. This review will discuss how to improve the conversion efficiency using pasture management to reduce or mitigate enteric methane production

Accreting Black Holes

This chapter provides a general overview of the theory and observations of black holes in the Universe and on their interpretation. We briefly review the black hole classes, accretion disk models, spectral state classification, the AGN classification, and the leading techniques for measuring black hole spins. We also introduce quasi-periodic oscillations, the shadow of black holes, and the observations and the theoretical models of jets.Comment: 41 pages, 18 figures. To appear in "Tutorial Guide to X-ray and Gamma-ray Astronomy: Data Reduction and Analysis" (Ed. C. Bambi, Springer Singapore, 2020). v3: fixed some typos and updated some parts. arXiv admin note: substantial text overlap with arXiv:1711.1025

Multivariate analysis reveals genetic diversity in Paspalum notatum Flügge

The objective of this study was to evaluate 94 Paspalum notatum genotypes over two growing seasons to estimate genetic dissimilarity through agronomic traits and the distance between genotypes. This information is used to create an ideotype from the best averages obtained for the set of characteristics evaluated. Seven apomitic, three sexual, and 81 hybrid genotypes were compared with native genotypes “André da Rocha”, “Bagual”, and cultivar “Pensacola” as controls. There is genetic variability in P. notatum for the studied characteristics, and distinct genotypes with superior characteristics can be used in new combinations between apomictic and sexual plants to obtain hybrids. The characters with the greatest relative contribution to the dissimilarity between the genotypes were tiller density, stem dry mass, and leaf dry mass yield. Thus, these characteristics are suitable criteria to infer genetic distance studies in P. notatum. The selection index based on the ideotype is an auxiliary tool in the breeding process. The ideotype must be based on characteristics of interest according to the objective of the breeding program, as well as on the breeder’s prior knowledge in relation to culture

Structural insights into Clostridium perfringens delta toxin pore formation

Clostridium perfringens Delta toxin is one of the three hemolysin-like proteins produced by C. perfringens type C and possibly type B strains. One of the others, NetB, has been shown to be the major cause of Avian Nectrotic Enteritis, which following the reduction in use of antibiotics as growth promoters, has become an emerging disease of industrial poultry. Delta toxin itself is cytotoxic to the wide range of human and animal macrophages and platelets that present GM2 ganglioside on their membranes. It has sequence similarity with Staphylococcus aureus β-pore forming toxins and is expected to heptamerize and form pores in the lipid bilayer of host cell membranes. Nevertheless, its exact mode of action remains undetermined. Here we report the 2.4 Å crystal structure of monomeric Delta toxin. The superposition of this structure with the structure of the phospholipid-bound F component of S. aureus leucocidin (LukF) revealed that the glycerol molecules bound to Delta toxin and the phospholipids in LukF are accommodated in the same hydrophobic clefts, corresponding to where the toxin is expected to latch onto the membrane, though the binding sites show significant differences. From structure-based sequence alignment with the known structure of staphylococcal α-hemolysin, a model of the Delta toxin pore form has been built. Using electron microscopy, we have validated our model and characterized the Delta toxin pore on liposomes. These results highlight both similarities and differences in the mechanism of Delta toxin (and by extension NetB) cytotoxicity from that of the staphylococcal pore-forming toxins

Genetic parameters, prediction of gains and intraspecific hybrid selection of Paspalum notatum Flügge for forage using REML/BLUP

Genetic improvement of native forage species is a sustainable alternative for maximizing livestock production. Paspalum notatum Flügge is the most important forage grass in the native grasslands of southern Brazil, with substantial potential available for further genetic improvement. The objective of this study was to quantify a range of genetic parameters and predict yield gains in a population of P. notatum intraspecific hybrids. Results indicated intraspecific hybrids of P. notatum had high magnitudes of heritability in the broad and average sense of genotype, plus high selective accuracy and genetic variation for all forage characteristics evaluated. This indicated REML/BLUP can contribute useful information for plant selection in future plant breeding programs. The genetic material studied showed high genetic variability for forage production. Analysis indicated hybrids 336, 332, 437, 132 and male parent '30N' should be included in new crosses to increase the dry matter production of P. notatum. Parents need to be selected from different groups in order to maximize genetic variability and heterosis. In addition, these parents must be included in diallel crosses. The results obtained in this study provide important information for the future breeding of improved P. notatum cultivars for commercialization

Accreting Millisecond X-Ray Pulsars

Accreting Millisecond X-Ray Pulsars (AMXPs) are astrophysical laboratories without parallel in the study of extreme physics. In this chapter we review the past fifteen years of discoveries in the field. We summarize the observations of the fifteen known AMXPs, with a particular emphasis on the multi-wavelength observations that have been carried out since the discovery of the first AMXP in 1998. We review accretion torque theory, the pulse formation process, and how AMXP observations have changed our view on the interaction of plasma and magnetic fields in strong gravity. We also explain how the AMXPs have deepened our understanding of the thermonuclear burst process, in particular the phenomenon of burst oscillations. We conclude with a discussion of the open problems that remain to be addressed in the future.Comment: Review to appear in "Timing neutron stars: pulsations, oscillations and explosions", T. Belloni, M. Mendez, C.M. Zhang Eds., ASSL, Springer; [revision with literature updated, several typos removed, 1 new AMXP added

Environmental regulation induced foreign direct investment

The last decade has witnessed a renewed interest in the relationship between environmental regulations and international capital flows. However, empirical studies have so far failed to find conclusive evidence for this so-called pollution haven or race to the bottom effect where foreign direct investment (FDI) is assumed to be attracted to low regulation countries, regions or states. In this paper we present a simple theoretical framework to demonstrate that greater stringency in environmental standards can lead to a strategic increase in capital inflows which we refer to as environmental regulation induced FDI. Our result reveals a possible explanation for the mixed results in the empirical literature and provides an illustration of the conditions under which environmental regulations in the host country can affect the location decision of foreign firms

High-throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity

Mainstream adoption of physiologically-relevant three-dimensional models has been slow in the last 50 years due to long, manual protocols with poor reproducibility, high price and closed commercial platforms. This chapter describes high-throughput, low-cost, open methods for spheroid viability assessment which use readily-available reagents and open-source software to analyse spheroid volume, metabolism and enzymatic activity. We provide two ImageJ macros for automated spheroid size determination - for both single images and for images in stacks. We also share an Excel template spreadsheet allowing users to rapidly process spheroid size data, analyse plate uniformity (such as edge effects and systematic seeding errors), detect outliers and calculate dose-response. The methods would be useful to researchers in preclinical and translational research planning to move away from simplistic monolayer studies and explore 3D spheroid screens for drug safety and efficacy without substantial investment in money or time