46 research outputs found

    A World Without Tom Staley: A Tribute

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    Coping with Joyce: essays from the Copenhagen symposium

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    Essays from the Tenth International James Joyce Symposium held in Copenhagen in 1986(print) xviii, 280 p. : port. ; 24 cmIntroduction ix -- Abbreviations xvii -- MAJOR ADDRESS -- 1. Joyce's Heliotrope Margot Norris 3 -- 2. Joyce the Verb Fritz Senn 25 -- 3. The Joycead Colbert Kearney 55 -- 4. Inscribing James Joyce's Tombstone Bernard Benstock 73 -- 5. Joyce and Modernist Ideology Robert Scholes 91 -- CRITICAL STUDIES -- 6. Farrington the Scrivener : A Story of Dame Street Morris Beja 111 -- 7. The Language of Exiles Give Hart 123 -- 8. And the Music Goes Round and Round : A Couple of New Approaches to Joyce's Uses of Music in Ulysses Zack Bowen 137 -- 9. "Roll Away the Reel World, the Reel World" : "Circe" and Cinema Austin Briggs 145 -- 10. Images of the Lacanian Gaze in Ulysses Sheldon Brivic 157 -- 11. Jellyfish and Treacle : Lewis, Joyce, Gender, and Modernism Bonnie Kime Scott 168 -- 12. The Letter Selfpenned to One's Other : Joyce's Writing, Deconstruction, Feminism Ellen Carol Jones 180 -- 13. Simulation, Pluralism, and the Politics of Everyday Life Jules David Law 195 -- 14. Joyce's Pedagogy: Ulysses and Finnegans Wake as Theory Patrick McGee 206 -- 15. From Catechism to Catachresis : Aspects of Joycean Pedagogy in Ulysses and Finnegans Wake Lorraine Weir 220 -- 16. ALP's Final Monologue in Finnegans Wake : The Dialectical Logic of Joyce's Dream Text Kimberly Devlin 232 -- 17. Shahrazade, Turko the Terrible, and Shem : The Reader as Voyeur in Finnegans Wake Henriette Lazaridis Power 248 -- 18. The Wakes Confounded Language Derek Attridge 262 -- Contributors 269 -- Index 27

    Joyce in the Hibernian metropolis: essays

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    (print) xx, 312 p. : ill. ; 23 cmCollection of essays from the 13th International James Joyce Symposium, held in Dublin, June 1992David Norris, Preface xi -- Acknowledgments xv -- Mary Robinson, Welcome Address xvii -- Abbreviations xix -- GENERAL ESSAYS -- Robert Adams Day, "Joyce's AquaCities" 3 -- Vincent J. Cheng, "Catching the Conscience of a Race: Joyce and Celticism" 21 -- David Norris, "OndtHarriet, PoldyLeon and Shem the Conman" 44 -- Jeffrey Segall, "Czech Ulysses: Joyce and Political Correctness, East and West" 52 -- Louis Lentin, "I Don't Understand. I Fail To Say. I Dearsee You Too" 61 -- HOSTILE RESPONSES TO JOYCE -- Morris Beja, "Approaching Joyce with an Attitude" 71 -- Paul Delany, "A Would-Be-Dirty Mind' : D. H. Lawrence as an Enemy of Joyce" 76 -- Austin Briggs, "Rebecca West vs. James Joyce, Samuel Beckett, and William Carlos Williams" 83 -- MALE FEMINISMS : APPROACHING "NAUSICAA" -- Richard Pearce, "Introduction" 105 -- Richard Pearce, "'Nausicaa' : Monologue as Monologic" 106 -- Philip Weinstein, "For Gerty Had Her Dreams that No-one Knew Of" 115 -- Patrick McGee, "When Is a Man Not a Man? or, The Male Feminist Approaches 'Nausicaa'" 122 -- Jennifer Levine, "'Nausicaa' : For [Wo]men Only?" 128 -- THE SHORTER WORKS -- Zack Bowen, "All Things Come in Threes : Manage a Trois in Dubliners" 137 -- James D. LeBlanc, "Duffy's Adventure : 'A Painful Case' as Existential Text" 144 -- Ruth Bauerle, "Dancing a Pas de Deux in Exiles's Menage a Quatre; or, How Many Triangles Can You Make Out of Four Characters If You Take Them Two at a Time?" 150 -- Adriaan van der Weel and Ruud Hisgen, "The Wandering Gentile : Joyce's Emotional Odyssey in Pomes Penyeach" 164 -- "AEOLUS" WITHOUT WIND -- Derek Attridge, "Introduction" 179 -- Jennifer Levine, "A Brief Allegory of Readings : 1972-1992" 181 -- Daniel Ferrer, "Between Irwentio and Memoria : Locations of 'Aeolus'" 190 -- Maud Ellmann, "'Aeolus' : Reading Backward" 198 -- THE NOVELS -- Sheldon Brivic, "Stephen Haunted by His Gender : The Uncanny Portrait" 205 -- Sebastian D. G. Knowles, "That Form Endearing : A Performance of Siren Songs; or, 'I was only vamping, man'" 213 -- Mark Osteen, "Cribs in the Countinghouse : Plagiarism, Proliferation, and Labor in 'Oxen of the Sun'" 237 -- John S. Rickard, "The Irish Undergrounds of Joyce and Heaney" 250 -- Thomas L. Burkdall, "Cinema Fakes : Film and Joycean Fantasy" 260 -- Ralph W. Rader, "Mulligan and Molly : The Beginning and the End" 270 -- Laurent Milesi, "Finnegans Wake : The Obliquity of Trans-lations" 279 -- Derek Attridge, "Countlessness of Livestories : Narrativity in Finnegans Wake" 290 -- Contributors 297 -- Index 30

    Function and Regulation of Vibrio campbellii Proteorhodopsin: Acquired Phototrophy in a Classical Organoheterotroph

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    Proteorhodopsins (PRs) are retinal-binding photoproteins that mediate light-driven proton translocation across prokaryotic cell membranes. Despite their abundance, wide distribution and contribution to the bioenergy budget of the marine photic zone, an understanding of PR function and physiological significance in situ has been hampered as the vast majority of PRs studied to date are from unculturable bacteria or culturable species that lack the tools for genetic manipulation. In this study, we describe the presence and function of a horizontally acquired PR and retinal biosynthesis gene cluster in the culturable and genetically tractable bioluminescent marine bacterium Vibrio campbellii. Pigmentation analysis, absorption spectroscopy and photoinduction assays using a heterologous over-expression system established the V. campbellii PR as a functional green light absorbing proton pump. In situ analyses comparing PR expression and function in wild type (WT) V. campbellii with an isogenic ΔpR deletion mutant revealed a marked absence of PR membrane localization, pigmentation and light-induced proton pumping in the ΔpR mutant. Comparative photoinduction assays demonstrated the distinct upregulation of pR expression in the presence of light and PR-mediated photophosphorylation in WT cells that resulted in the enhancement of cellular survival during respiratory stress. In addition, we demonstrate that the master regulator of adaptive stress response and stationary phase, RpoS1, positively regulates pR expression and PR holoprotein pigmentation. Taken together, the results demonstrate facultative phototrophy in a classical marine organoheterotrophic Vibrio species and provide a salient example of how this organism has exploited lateral gene transfer to further its adaptation to the photic zone

    Influence of nutrients and currents on the genomic composition of microbes across an upwelling mosaic

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    Metagenomic data sets were generated from samples collected along a coastal to open ocean transect between Southern California Bight and California Current waters during a seasonal upwelling event, providing an opportunity to examine the impact of episodic pulses of cold nutrient-rich water into surface ocean microbial communities. The data set consists of ∼5.8 million predicted proteins across seven sites, from three different size classes: 0.1–0.8, 0.8–3.0 and 3.0–200.0 μm. Taxonomic and metabolic analyses suggest that sequences from the 0.1–0.8 μm size class correlated with their position along the upwelling mosaic. However, taxonomic profiles of bacteria from the larger size classes (0.8–200 μm) were less constrained by habitat and characterized by an increase in Cyanobacteria, Bacteroidetes, Flavobacteria and double-stranded DNA viral sequences. Functional annotation of transmembrane proteins indicate that sites comprised of organisms with small genomes have an enrichment of transporters with substrate specificities for amino acids, iron and cadmium, whereas organisms with larger genomes have a higher percentage of transporters for ammonium and potassium. Eukaryotic-type glutamine synthetase (GS) II proteins were identified and taxonomically classified as viral, most closely related to the GSII in Mimivirus, suggesting that marine Mimivirus-like particles may have played a role in the transfer of GSII gene functions. Additionally, a Planctomycete bloom was sampled from one upwelling site providing a rare opportunity to assess the genomic composition of a marine Planctomycete population. The significant correlations observed between genomic properties, community structure and nutrient availability provide insights into habitat-driven dynamics among oligotrophic versus upwelled marine waters adjoining each other spatially

    Metagenomics of the Deep Mediterranean, a Warm Bathypelagic Habitat

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    BACKGROUND: Metagenomics is emerging as a powerful method to study the function and physiology of the unexplored microbial biosphere, and is causing us to re-evaluate basic precepts of microbial ecology and evolution. Most marine metagenomic analyses have been nearly exclusively devoted to photic waters. METHODOLOGY/PRINCIPAL FINDINGS: We constructed a metagenomic fosmid library from 3,000 m-deep Mediterranean plankton, which is much warmer (approximately 14 degrees C) than waters of similar depth in open oceans (approximately 2 degrees C). We analyzed the library both by phylogenetic screening based on 16S rRNA gene amplification from clone pools and by sequencing both insert extremities of ca. 5,000 fosmids. Genome recruitment strategies showed that the majority of high scoring pairs corresponded to genomes from Rhizobiales within the Alphaproteobacteria, Cenarchaeum symbiosum, Planctomycetes, Acidobacteria, Chloroflexi and Gammaproteobacteria. We have found a community structure similar to that found in the aphotic zone of the Pacific. However, the similarities were significantly higher to the mesopelagic (500-700 m deep) in the Pacific than to the single 4000 m deep sample studied at this location. Metabolic genes were mostly related to catabolism, transport and degradation of complex organic molecules, in agreement with a prevalent heterotrophic lifestyle for deep-sea microbes. However, we observed a high percentage of genes encoding dehydrogenases and, among them, cox genes, suggesting that aerobic carbon monoxide oxidation may be important in the deep ocean as an additional energy source. CONCLUSIONS/SIGNIFICANCE: The comparison of metagenomic libraries from the deep Mediterranean and the Pacific ALOHA water column showed that bathypelagic Mediterranean communities resemble more mesopelagic communities in the Pacific, and suggests that, in the absence of light, temperature is a major stratifying factor in the oceanic water column, overriding pressure at least over 4000 m deep. Several chemolithotrophic metabolic pathways could supplement organic matter degradation in this most depleted habitat

    Genomic insights to SAR86, an abundant and uncultivated marine bacterial lineage

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    Bacteria in the 16S rRNA clade SAR86 are among the most abundant uncultivated constituents of microbial assemblages in the surface ocean for which little genomic information is currently available. Bioinformatic techniques were used to assemble two nearly complete genomes from marine metagenomes and single-cell sequencing provided two more partial genomes. Recruitment of metagenomic data shows that these SAR86 genomes substantially increase our knowledge of non-photosynthetic bacteria in the surface ocean. Phylogenomic analyses establish SAR86 as a basal and divergent lineage of γ-proteobacteria, and the individual genomes display a temperature-dependent distribution. Modestly sized at 1.25–1.7 Mbp, the SAR86 genomes lack several pathways for amino-acid and vitamin synthesis as well as sulfate reduction, trends commonly observed in other abundant marine microbes. SAR86 appears to be an aerobic chemoheterotroph with the potential for proteorhodopsin-based ATP generation, though the apparent lack of a retinal biosynthesis pathway may require it to scavenge exogenously-derived pigments to utilize proteorhodopsin. The genomes contain an expanded capacity for the degradation of lipids and carbohydrates acquired using a wealth of tonB-dependent outer membrane receptors. Like the abundant planktonic marine bacterial clade SAR11, SAR86 exhibits metabolic streamlining, but also a distinct carbon compound specialization, possibly avoiding competition
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