Computational purification of individual tumor gene expression profiles leads to significant improvements in prognostic prediction.

Tumor heterogeneity is a limiting factor in cancer treatment and in the discovery of biomarkers to personalize it. We describe a computational purification tool, ISOpure, to directly address the effects of variable normal tissue contamination in clinical tumor specimens. ISOpure uses a set of tumor expression profiles and a panel of healthy tissue expression profiles to generate a purified cancer profile for each tumor sample and an estimate of the proportion of RNA originating from cancerous cells. Applying ISOpure before identifying gene signatures leads to significant improvements in the prediction of prognosis and other clinical variables in lung and prostate cancer

Porous silica spheres as indoor air pollutant scavengers

Porous silica spheres were investigated for their effectiveness in removing typical indoor air pollutants, such as aromatic and carbonyl-containing volatile organic compounds (VOCs), and compared to the commercially available polymer styrene-divinylbenzene (XAD-4). The silica spheres and the XAD-4 resin were coated on denuder sampling devices and their adsorption efficiencies for volatile organic compounds evaluated using an indoor air simulation chamber. Real indoor sampling was also undertaken to evaluate the affinity of the silica adsorbents for a variety of indoor VOCs. The silica sphere adsorbents were found to have a high affinity for polar carbonyls and found to be more efficient than the XAD-4 resin at adsorbing carbonyls in an indoor environment

Properties of Hot Stars in the Wolf-Rayet galaxy NGC5253 from ISO Spectroscopy

ISO-SWS spectroscopy of the WR galaxy NGC5253 is presented, and analysed to provide estimates of its hot young star population. Our approach differs from previous investigations in that we are able to distinguish between the regions in which different infrared fine-structure lines form, using complementary ground-based observations. The high excitation nebular [SIV] emission is formed in a very compact region, which we attribute to the central super-star-nucleus, and lower excitation [NeII] nebular emission originates in the galactic core. We use photo-ionization modelling coupled with the latest theoretical O-star flux distributions to derive effective stellar temperatures and ionization parameters of Teff>38kK, logQ=8.25 for the compact nucleus, with Teff=35kK, logQ<8 for the larger core. Results are supported by more sophisticated calculations using evolutionary synthesis models. We assess the contribution that Wolf-Rayet stars may make to highly ionized nebular lines (e.g. [OIV]). From our Br(alpha) flux, the 2" nucleus contains the equivalent of approximately 1000 O7V star equivalents and the starburst there is 2-3Myr old; the 20" core contains about 2500 O7V star equivalents, with a representative age of 5Myr. The Lyman ionizing flux of the nucleus is equivalent to the 30 Doradus region. These quantities are in good agreement with the observed mid-IR dust luminosity of 7.8x10^8 L(sun) Since this structure of hot clusters embedded in cooler emission may be common in dwarf starbursts, observing a galaxy solely with a large aperture may result in confusion. Neglecting the spatial distribution of nebular emission in NGC5253, implies `global' stellar temperatures (or ages) of 36kK (4.8Myr) and 39kK (2.9 or 4.4Myr) from the observed [NeIII/II] and [SIV/III] line ratios, assuming logQ=8.Comment: 16 pages, 7 figures, uses mn.sty, to appear in MNRA

Plantain (\u3cem\u3ePlantago lanceolata\u3c/em\u3e) in Herb and Legume Pastures Increases Lamb Growth Relative to Perennial Ryegrass and White Clover Pasture

Increased use of plantain (Plantago lanceolata) by New Zealand farmers has created a demand for a more complete understanding of the performance and manage-ment of plantain by itself and in various combinations with chicory (Cichorium intybus) and legumes such as white clover (Trifolium repens) and red clover (T. pratense). The objective of this study was to evaluate lamb finishing on plantain by itself and combined with chicory, white and red clover as part of a research programme to develop a temperate perennial pasture superior in animal performance to perennial ryegrass and white clover pasture for sheep production systems (Kemp et al. 2010)