Post-mortem diagnosis of kidney impairment:an experimental study

The determination of the role that drugs may have played in a death is an important part of the investigation into unexplained deaths. Renal impairment may lead to a reduction in drug excretion rate and therefore an accumulation of drugs or metabolites, leading to possible toxic or lethal effects. Creatinine levels are known to be stable in the post mortem period and in life can give an indication of kidney function. There are however widely reported limitations when using creatinine in isolation and so we investigated the usefulness of using estimated glomerular filtration rate (eGFR) for scoring an individual as having renal impairment using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula. We analysed unpreserved vitreous for creatinine in 812 individuals using an isotope dilution mass spectrometry (ID-MS) traceable enzymatic. We found that the biochemical analysis of post mortem vitreous creatinine and subsequent calculation of eGFR is a useful adjunct to the standard testing that takes place during a post-mortem examination and can assist in death investigation. Using an eGFR of <60 mL/min/1.73 m2 gave a sensitivity of 94.3% and specificity of 97.3% when scoring an individual as having renal impairment. We therefore recommend the calculation of eGFR for the determination of possible renal impairment in post mortem investigations. It is, of course, always pertinent to interpret any results using a wealth of case information. Extreme caution should be exercised in cases where insufficient clinical information/history is available, particularly in cases in which there is suspected diabetic ketoacidosis, dehydration or hospitalisation prior to death

Mesoscopic Interference for Metric and Curvature (MIMAC) & Gravitational Wave Detection

A compact detector for space-time metric and curvature is highly desirable. Here we show that quantum spatial superpositions of mesoscopic objects, of the type which would in principle become possible with a combination of state of the art techniques and taking into account the known sources of decoherence, could be exploited to create such a detector. By using Stern-Gerlach (SG) interferometry with masses much larger than atoms, where the interferometric signal is extracted by measuring spins, we show that accelerations as low as $5\times10^{-15}\textrm{ms}^{-2}\textrm{Hz}^{-1/2}$ or better, as well as the frame dragging effects caused by the Earth, could be sensed. Constructing such an apparatus to be non-symmetric would also enable the direct detection of curvature and gravitational waves (GWs). The GW sensitivity scales differently from the stray acceleration sensitivity, a unique feature of MIMAC. We have identified mitigation mechanisms for the known sources of noise, namely Gravity Gradient Noise (GGN), uncertainty principle and electro-magnetic forces. Hence it could potentially lead to a meter sized, orientable and vibrational noise (thermal/seismic) resilient detector of mid (ground based) and low (space based) frequency GWs from massive binaries (the predicted regimes are similar to those targeted by atom interferometers and LISA).Comment: 29 pages, 3 figure

Deep three-dimensional solid-state qubit arrays with long-lived spin coherence

Nitrogen-vacancy centers (NVCs) in diamond show promise for quantum computing, communication, and sensing. However, the best current method for entangling two NVCs requires that each one is in a separate cryostat, which is not scalable. We show that single NVCs can be laser written 6–15-µm deep inside of a diamond with spin coherence times that are an order of magnitude longer than previous laser-written NVCs and at least as long as naturally occurring NVCs. This depth is suitable for integration with solid immersion lenses or optical cavities and we present depth-dependent T2 measurements. 200 000 of these NVCs would fit into one diamond

The Interaction of Neuraminidase and Hemagglutinin Mutations in Influenza Virus in Resistance to 4-Guanidino-Neu5Ac2en

AbstractWe have previously described a 4-guanidino-Neu5Ac2en (zanamivir)-resistant neuraminidase (NA) variant G70C4-G, with an active site mutation Glu 119 to Gly. This variant has been found to also harbor a hemagglutinin (HA) mutation in the receptor binding site, Ser 186 to Phe. Examination of early passages of the G70C4-G virus revealed that this HA mutation had arisen by the first passage. From a subsequent passage two transient variants were isolated which had each acquired a different second HA mutation, Ser 165 to Asn and Lys 222 to Thr. Both were highly drug resistant and drug dependent and their ability to adsorb to and penetrate cells was decreased. Comparison of drug sensitivities between the variant, with the additional HA mutation at Ser 165, and viruses with either mutation alone revealed that these two HA mutations acted synergistically to increase resistance. To determine the contribution to resistance of each of the NA and HA mutations in G70C4-G, the NA mutation was separated from the HA mutation by reassorting. The NA mutation and the HA mutation each conferred low-level resistance to zanamivir, while the two mutations interacted synergistically in the double mutant to give higher resistancein vitro.Infectivity was not adversely affected in the double mutant and while there was a small decrease in sensitivity to zanamivir in the mouse model, there was no detectable resistance to zanamivir in the ferret model

An HST/STIS Optical Transmission Spectrum of Warm Neptune GJ 436b

GJ 436b is a prime target for understanding warm Neptune exoplanet atmospheres and a target for multiple JWST GTO programs. Here, we report the first space-based optical transmission spectrum of the planet using two HST/STIS transit observations from 0.53-1.03 microns. We find no evidence for alkali absorption features, nor evidence of a scattering slope longward of 0.53 microns. The spectrum is indicative of moderate to high metallicity (~100-1000x solar) while moderate metallicity scenarios (~100x solar) require aerosol opacity. The optical spectrum also rules out some highly scattering haze models. We find an increase in transit depth around 0.8 microns in the transmission spectra of 3 different sub-Jovian exoplanets (GJ 436b, HAT-P-26b, and GJ 1214b). While most of the data come from STIS, data from three other instruments may indicate this is not an instrumental effect. Only the transit spectrum of GJ 1214b is well fit by a model with stellar plages on the photosphere of the host star. Our photometric monitoring of the host star reveals a stellar rotation rate of 44.1 days and an activity cycle of 7.4 years. Intriguingly, GJ 436 does not become redder as it gets dimmer, which is expected if star spots were dominating the variability. These insights into the nature of the GJ 436 system help refine our expectations for future observations in the era of JWST, whose higher precision and broader wavelength coverage will shed light on the composition and structure of GJ 436b's atmosphere.Comment: 20 pages, 11 figures, 5 tables, Accepted to AJ. A full version of table 1 is included as table1_mrt.tx

Pendulum Mode Thermal Noise in Advanced Interferometers: A comparison of Fused Silica Fibers and Ribbons in the Presence of Surface Loss

The use of fused-silica ribbons as suspensions in gravitational wave interferometers can result in significant improvements in pendulum mode thermal noise. Surface loss sets a lower bound to the level of noise achievable, at what level depends on the dissipation depth and other physical parameters. For LIGO II, the high breaking strength of pristine fused silica filaments, the correct choice of ribbon aspect ratio (to minimize thermoelastic damping), and low dissipation depth combined with the other achievable parameters can reduce the pendulum mode thermal noise in a ribbon suspension well below the radiation pressure noise. Despite producing higher levels of pendulum mode thermal noise, cylindrical fiber suspensions provide an acceptable alternative for LIGO II, should unforeseen problems with ribbon suspensions arise.Comment: Submitted to Physics Letters A (Dec. 14, 1999). Resubmitted to Physics Letters A (Apr. 3, 2000) after internal (LSC) review process. PACS - 04.80.Nn, 95.55.Ym, 05.40.C

Caloric curves and critical behavior in nuclei

Data from a number of different experimental measurements have been used to construct caloric curves for five different regions of nuclear mass. These curves are qualitatively similar and exhibit plateaus at the higher excitation energies. The limiting temperatures represented by the plateaus decrease with increasing nuclear mass and are in very good agreement with results of recent calculations employing either a chiral symmetry model or the Gogny interaction. This agreement strongly favors a soft equation of state. Evidence is presented that critical excitation energies and critical temperatures for nuclei can be determined over a large mass range when the mass variations inherent in many caloric curve measurements are taken into account.Comment: In response to referees comments we have improved the discussion of the figures and added a new figure showing the relationship between the effective level density and the excitation energy. The discussion has been reordered and comments are made on recent data which support the hypothesis of a mass dependence of caloric curve