Changes in the genetic requirements for microbial interactions with increasing community complexity.

Microbial community structure and function rely on complex interactions whose underlying molecular mechanisms are poorly understood. To investigate these interactions in a simple microbiome, we introduced E. coli into an experimental community based on a cheese rind and identified the differences in E. coli's genetic requirements for growth in interactive and non-interactive contexts using Random Barcode Transposon Sequencing (RB-TnSeq) and RNASeq. Genetic requirements varied among pairwise growth conditions and between pairwise and community conditions. Our analysis points to mechanisms by which growth conditions change as a result of increasing community complexity and suggests that growth within a community relies on a combination of pairwise and higher-order interactions. Our work provides a framework for using the model organism E. coli as a readout to investigate microbial interactions regardless of the genetic tractability of members of the studied ecosystem

The "Binarity and Magnetic Interactions in various classes of Stars" (BinaMIcS) project

The "Binarity and Magnetic Interactions in various classes of stars" (BinaMIcS) project is based on two large programs of spectropolarimetric observations with ESPaDOnS at CFHT and Narval at TBL. Three samples of spectroscopic binaries with two spectra (SB2) are observed: known cool magnetic binaries, the few known hot magnetic binaries, and a survey sample of hot binaries to search for additional hot magnetic binaries. The goal of BinaMIcS is to understand the complex interplay between stellar magnetism and binarity. To this aim, we will characterise and model the magnetic fields, magnetospheric structure and coupling of both components of hot and cool close binary systems over a significant range of evolutionary stages, to confront current theories and trigger new ones. First results already provided interesting clues, e.g. about the origin of magnetism in hot stars.Comment: 4 pages, 2 figures, proceedings of the SF2A conferenc

Preferred levels for background ducking to produce esthetically pleasing audio for TV with clear speech

In audio production, background ducking facilitates speech intelligibility while allowing the background to fulfill its purpose, e.g., to create ambience, set the mood, or convey semantic cues. Technical details for recommended ducking practices are not currently documented in the literature. Hence, we first analyzed common practices found in TV documentaries. Second, a listening test investigated the preferences of 22 normal-hearing participants on the Loud- ness Difference (LD) between commentary and background during ducking. Highly personal preferences were observed, highlighting the importance of object-based personalization. Sta- tistically significant difference was found between non-expert and expert listeners. On average, non-experts preferred LDs that were 4 LU higher than the ones preferred by experts. A sta- tistically significant difference was also found between Commentary over Music (CoM) and Commentary over Ambience (CoA). Based on the test results, we recommend at least 10 LU difference for CoM and at least 15 LU for CoA. Moreover, a computational method based on the Binaural Distortion-Weighted Glimpse Proportion (BiDWGP) was found to match the median preferred LD for each item with good accuracy (mean absolute error = 1.97 LU ± 2.50)

Life cycle studies of the hexose transporter of Plasmodium species and genetic validation of their essentiality

A Plasmodium falciparum hexose transporter (PfHT) has previously been shown to be a facilitative glucose and fructose transporter. Its expression in Xenopus laevis oocytes and the use of a glucose analogue inhibitor permitted chemical validation of PfHT as a novel drug target. Following recent re-annotations of the P. falciparum genome, other putative sugar transporters have been identified. To investigate further if PfHT is the key supplier of hexose to P. falciparum and to extend studies to different stages of Plasmodium spp., we functionally analysed the hexose transporters of both the human parasite P. falciparum and the rodent parasite Plasmodium berghei using gene targeting strategies. We show here the essential function of pfht for the erythrocytic parasite growth as it was not possible to knockout pfht unless the gene was complemented by an episomal construct. Also, we show that parasites are rescued from the toxic effect of a glucose analogue inhibitor when pfht is overexpressed in these transfectants. We found that the rodent malaria parasite orthologue, P. berghei hexose transporter (PbHT) gene, was similarly refractory to knockout attempts. However, using a single cross-over transfection strategy, we generated transgenic P. berghei parasites expressing a PbHT–GFP fusion protein suggesting that locus is amenable for gene targeting. Analysis of pbht-gfp transgenic parasites showed that PbHT is constitutively expressed through all the stages in the mosquito host in addition to asexual stages. These results provide genetic support for prioritizing PfHT as a target for novel antimalarials that can inhibit glucose uptake and kill parasites, as well as unveiling the expression of this hexose transporter in mosquito stages of the parasite, where it is also likely to be critical for survival

The evolution of surface magnetic fields in young solar-type stars

The surface rotation rates of young solar-type stars decrease rapidly with age from the end of the pre-main sequence though the early main sequence. This suggests that there is also an important change in the dynamos operating in these stars, which should be observable in their surface magnetic fields. Here we present early results in a study aimed at observing the evolution of these magnetic fields through this critical time period. We are observing stars in open clusters and stellar associations to provide precise ages, and using Zeeman Doppler Imaging to characterize the complex magnetic fields. Presented here are results for six stars, three in the in the beta Pic association (~10 Myr old) and three in the AB Dor association (~100 Myr old).Comment: To appear in the proceedings of IAU symposium 302: Magnetic fields throughout stellar evolution. 2 pages, 3 figure

Congenital dislocation of the hip, with late diagnosis after 1year of age: Update and management

SummaryWhen congenital dislocation of the hip (CDH) is diagnosed only after walking age, management is greatly complicated. In view of the increasing number of such cases treated by members of the French Society of Paediatric Orthopaedics (SOFOP) in recent years, a 3-part study was conducted within the Society to shed light on the reasons for late diagnosis, to give an update on treatment strategies currently implemented in France, and to report long-term outcomes

Donor Electron Wave Functions for Phosphorus in Silicon: Beyond Effective Mass Theory

We calculate the electronic wave-function for a phosphorus donor in silicon by numerical diagonalisation of the donor Hamiltonian in the basis of the pure crystal Bloch functions. The Hamiltonian is calculated at discrete points localised around the conduction band minima in the reciprocal lattice space. Such a technique goes beyond the approximations inherent in the effective-mass theory, and can be modified to include the effects of altered donor impurity potentials, externally applied electro-static potentials, as well as the effects of lattice strain. Modification of the donor impurity potential allows the experimentally known low-lying energy spectrum to be reproduced with good agreement, as well as the calculation of the donor wavefunction, which can then be used to calculate parameters important to quantum computing applications.Comment: 10 pages, 5 figure

Modeling the RV jitter of early M dwarfs using tomographic imaging

In this paper we show how tomographic imaging (Zeeman Doppler Imaging, ZDI) can be used to characterize stellar activity and magnetic field topologies, ultimately allowing to filter out the radial velocity (RV) activity jitter of M-dwarf moderate rotators. This work is based on spectropolarimetric observations of a sample of five weakly-active early M-dwarfs (GJ 205, GJ 358, GJ 410, GJ479, GJ 846) with HARPS-Pol and NARVAL. These stars have v sin i and RV jitters in the range 1-2 km/s and 2.7-10.0 m/s rms respectively. Using a modified version of ZDI applied to sets of phase-resolved Least-Squares- Decon- volved (LSD) profiles of unpolarized spectral lines, we are able to characterize the distribution of active regions at the stellar surfaces. We find that darks spots cover less than 2% of the total surface of the stars of our sample. Our technique is e cient at modeling the rotationally mod- ulated component of the activity jitter, and succeeds at decreasing the amplitude of this com- ponent by typical factors of 2-3 and up to 6 in optimal cases. From the rotationally modulated time-series of circularly polarized spectra and with ZDI, we also reconstruct the large-scale magnetic field topology. These fields suggest that bi-stability of dynamo processes observed in active M dwarfs may also be at work for moderately active M dwarfs. Comparing spot distributions with field topologies suggest that dark spots causing activity jitter concentrate at the magnetic pole and/or equator, to be confirmed with future data on a larger sample.Comment: 34 pages, accepted for publication in MNRA