DeMon++: A framework for designing and implementing Distributed Monitoring Systems based on Hierarchical Finite State Machines

In today’s interconnected world, the proliferation of diverse and numerous devices has become increasingly common. This phenomenon is particularly evident in the field of industrial computing, which has experienced rapid growth. With this rapid expansion, monitoring an industrial control system (ICS) consisting of a large num- ber of devices becomes a critical activity. To evaluate our approach, we chose the CERN ICS as a suitable case study for our research. The CERN ICS is a complex network of thousands of heterogeneous control devices, including PLCs, front-end computers, supervisory control and data acquisition systems. Our approach resulted in DeMon++, a framework for designing and implementing distributed monitoring systems. DeMon++ uses the concept of hierarchical finite state machines to model the system, capturing the hierarchical relationship between devices. In particular, DeMon++ aims to be a flexible, scalable and maintainable monitoring framework to abstract, aggregate and summarise the health state of industrial control sys- tems composed of a heterogeneous set of devices. As part of the CERN OpenLab programme, this thesis provides a flexible and maintainable approach to monitoring complex and distributed ICS, with a particular focus on the demanding environment of CERN

IC 5181: An S0 Galaxy with Ionized Gas on Polar Orbits

The nearby S0 galaxy IC 5181 is studied to address the origin of the ionized gas component that orbits the galaxy on polar orbit. We perform detailed photometric and spectroscopic observations measuring the surface brightness distribution of the stars (I-band), ionized gas of IC 5181 (H-alpha narrow band), the ionized-gas and stellar kinematics along both the major and minor axis, and the corresponding line strengths of the Lick indices. We conclude that the galaxy hosts a geometrically and kinematically decoupled component of ionized gas. It is elongated along the galaxy minor axis and in orthogonal rotation with respect to the galaxy disk. The result is suggesting that the gas component is not related to the stars having an external origin. The gas was accreted by IC 5181 on polar orbits from the surrounding environment.Comment: 4 pages, 5 figures, To appear in ASP Conf. Ser., Multi-Spin Galaxies, E. Iodice and E. M. Corsini (eds.

Characterization of Some Stilbenoids Extracted from Two Cultivars of Lambrusco-Vitis vinifera Species: An Opportunity to Valorize Pruning Canes for a More Sustainable Viticulture

Pruning canes from grape vines are valuable byproducts that contain resveratrol and other health-boosting stilbenoids. This study aimed to assess the effect of roasting temperature on the stilbenoid content of vine canes by comparing two Vitis vinifera cultivars, Lambrusco Ancellotta and Salamino. Samples were collected during different phases of the vine plant cycle. One set was collected in September after the grape harvest and was air-dried and analyzed. A second set was obtained during vine pruning in February and evaluated immediately after collection. The main stilbenoid identified in each sample was resveratrol (similar to 100-2500 mg/kg), with significant levels of viniferin (similar to 100-600 mg/kg) and piceatannol (similar to 0-400 mg/kg). Their contents decreased with increasing roasting temperature and residence time on the plant. This study provides valuable insights into the use of vine canes in a novel and efficient manner, which could potentially benefit different industries. One potential use involves the roasted cane chips to accelerate the aging of vinegars and alcoholic beverages. This method is more efficient and cost-effective than traditional aging, which is slow and unfavorable from an industrial perspective. Furthermore, incorporating vine canes into maturation processes reduces viticulture waste and enhances the final products with health-promoting molecules, such as resveratrol

Performance and genome-centric metagenomics of thermophilic single and two-stage anaerobic digesters treating cheese wastes

The present research is the first comprehensive study regarding the thermophilic anaerobic degradation of cheese wastewater, which combines the evaluation of different reactor configurations (i.e. single and two-stage continuous stirred tank reactors) on the process efficiency and the in-depth characterization of the microbial community structure using genome-centric metagenomics. Both reactor configurations showed acidification problems under the tested organic loading rates (OLRs) of 3.6 and 2.4 g COD/L-reactor day and the hydraulic retention time (HRT) of 15 days. However, the two-stage design reached a methane yield equal to 95% of the theoretical value, in contrast with the single stage configuration, which reached a maximum of 33% of the theoretical methane yield. The metagenomic analysis identified 22 new population genomes and revealed that the microbial compositions between the two configurations were remarkably different, demonstrating a higher methanogenic biodiversity in the two-stage configuration. In fact, the acidogenic reactor of the serial configuration was almost solely composed by the lactose degrader Bifidobacterium crudilactis UC0001. The predictive functional analyses of the main population genomes highlighted specific metabolic pathways responsible for the AD process and the mechanisms of main intermediates production. Particularly, the acetate accumulation experienced by the single stage configuration was mainly correlated to the low abundant syntrophic acetate oxidizer Tepidanaerobacter acetatoxydans UC0018 and to the absence of aceticlastic methanogens

A catalogue of nuclear stellar velocity dispersions of nearby galaxies from \u2009H\u3b1 STIS spectra to constrain supermassive black hole masses

We present new measurements for the nuclear stellar velocity dispersion \u3c3* within sub-arcsecond apertures for 28 nearby galaxies. Our data consist of Space Telescope Imaging Spectrograph (STIS) long-slit spectra obtained with the G750M grating centred on the H\u3b1 spectral range. We fit the spectra using a library of single stellar population models and Gaussian emission lines, while constraining in most cases the stellar-population content from an initial fit to G430L STIS spectra. We illustrate how these \u3c3* measurements can be useful for constraining the mass M\u2022 of supermassive black holes (SBHs) by concentrating on the cases of the lenticular galaxies NGC 4435 and NGC 4459. These are characterized by similar ground-based half-light radii stellar velocity dispersion \u3c3e values but remarkably different M\u2022 as obtained from modelling their central ionized-gas kinematics, where NGC 4435 appears to host a significantly undermassive SBH compared to what is expected from the M\u2022 - \u3c3e relation. For both galaxies, we build Jeans axisymmetric dynamical models to match the ground-based stellar kinematics obtained with Spectrographic Areal Unit for Research on Optical Nebulae integral-field spectrograph, including an SBH with M\u2022 value as predicted by the M\u2022 - \u3c3e relation and using high-resolution HST images taken with the Advanced Camera for Surveys to construct the stellar-mass model. By mimicking the HST observing conditions we use such reference models to make a prediction for the nuclear \u3c3* value. Whereas this was found to agree with our nuclear \u3c3* measurement for NGC 4459, for NGC 4435 the observed \u3c3* is remarkably smaller than the predicted one, which further suggests that this galaxy could host an undermassive SBH

Effect of conjugated bile salts on antibiotic susceptibility of bile salt-tolerant Lactobacillus and Bifidobacterium isolates.

Virtually every antibiotic may cause in vivo alterations in the number, level, and composition of the indigenous microbiotae. The degree to which the microbiotae are disturbed depends on many factors. Although bile may augment antibiotic activity, studies on the effect of bile on the antibiotic susceptibility of indigenous and exogenous probiotic microorganisms are lacking. It was against this background that the antibiotic susceptibility of 37 bile salt-tolerant Lactobacillus and 11 Bifidobacterium isolates from human and other sources was determined in the presence of 0.5% wt/wt oxgall (conjugated bile salts). Oxgall did not affect the intrinsic resistance of lactobacilli to metronidazole (5 microg), vancomycin (30 microg), and cotrimoxazole (25 microg), whereas it resulted in a complete loss of resistance to polymyxin B (300 microg) and the aminoglycosides gentamicin (10 microg), kanamycin (30 microg), and streptomycin (10 microg) for most strains studied (P < 0.001). Oxgall did not affect the intrinsic resistance of bifidobacteria to metronidazole and vancomycin, whereas polymyxin B and co-trimoxazole resistance was diminished (P < 0.05) and aminoglycoside resistance was lost (P < 0.001). Seven lactobacilli, but no bifidobacteria strain, showed unaltered intrinsic antibiotic resistance profiles in the presence of oxgall. Oxgall affected the extrinsic susceptibility of lactobacilli and bifidobacteria to penicillin G (10 microg), ampicillin (10 microg), tetracycline (30 microg), chloramphenicol (30 microg), erythromycin (15 microg), and rifampicin (5 microg) in a source- and strain-dependent manner. Human strain-drug combinations of lactobacilli (P < 0.05) and bifidobacteria (P < 0.01) were more likely to show no change or decreased susceptibility compared with other strain-drug combinations. The antimicrobial activity spectra of polymyxin B and the aminoglycosides should not be considered limited to gram-negative bacteria but extended to include gram-positive genera of the indigenous and transiting microbiotae in the presence of conjugated bile salts. Those lactobacilli (7 of 37) that show unaltered intrinsic and diminished extrinsic antibiotic susceptibility in the presence of oxgall may possess greater upper gastrointestinal tract transit tolerance in the presence of antibiotics

Antibiotic susceptibility of potentially probiotic Lactobacillus species.

In recent years, the time-honored reputation of lactobacilli as promoters of gastrointestinal and female urogenital health has been qualified. This has occurred due to a rare association with human infection in the presence of certain predisposing factors and their potential to act as a source of undesirable antibiotic resistance determinants to other members of the indigenous microbiota. This necessitates greater caution in their selection for use in microbial adjunct nutrition and disease management (prophylaxis and therapy). It was against this background that 46 Lactobacillus strains from human and dairy sources were assayed for susceptibility to 44 antibiotics. All strains were resistant to a group of 14 antibiotics, which included inhibitors of cell wall synthesis (cefoxitin [30 microg] and aztreonam [30 microg]), protein synthesis (amikacin [30 microg], gentamicin [10 microg], kanamycin [30 microg], and streptomycin [10 microg]), nucleic acid synthesis (norfloxacin [10 microg], nalidixic acid [30 microg], sulphamethoxazole [100 microg], trimethoprim [5 microg], co-trimoxazole [25 microg], and metronidazole [5 microg]), and cytoplasmic membrane function (polymyxin B [300 microg] and colistin sulphate [10 microg]). All strains were susceptible to tetracycline (30 microg), chloramphenicol (30 microg), and rifampicin (5 microg). Four human strains and one dairy strain exhibited atypical resistance to a penicillin, bacitracin (10 microg), and/or nitrofurantoin (300 microg). One human strain was also resistant to erythromycin (15 microg) and clindamycin (2 microg). These resistances may have been acquired due to antibiotic exposure in vivo, but conclusive evidence is lacking in this regard. Seven microorganism-drug combinations were evaluated for beta-lactamase activity using synergy and nitrocefin tests. The absence of activity suggested that cell wall impermeability appeared responsible for beta-lactam resistance. The occurrence of a minority of lactobacilli with undesirable, atypical resistance to certain antibiotics demonstrates that not all strains are suitable for use as probiotics or bacteriotherapeutic agents. The natural resistance of lactobacilli to a wide range of clinically important antibiotics may enable the development of antibiotic/probiotic combination therapies for such conditions as diarrhea, female urogenital tract infection, and infective endocarditis

The properties and the formation mechanism of the stellar counter-rotating components in NGC 4191

We disentangle two counter-rotating stellar components in NGC 4191 and characterize their physical properties (kinematics, morphology, age, metallicity, and abundance ratio). We performed a spectroscopic decomposition on integral field data to separate the contribution of two stellar components to the observed galaxy spectrum across the field of view. We also performed a photometric decomposition, modelling the galaxy with a S\'ersic bulge and two exponential disks of different scale length, with the aim of associating these structural components with the kinematic components. We measured the equivalent width of the absorption line indices on the best fit that represent the kinematic components and compared our measurements to the predictions of stellar population models. We have evidence that the line-of-sight velocity distributions (LOSVDs) are consistent with the presence of two distinct kinematic components. The combined information of the intensity of the LOSVDs and photometry allows us to associate the S\'ersic bulge and the outer disk with the main kinematic component, and the inner disk with the secondary kinematic component. The two kinematic stellar components counter-rotate with respect to each other. The main component is the most luminous and massive, and it rotates slower than the secondary component, which rotates along the same direction as the ionized gas. We also found that the two kinematic components have the same solar metallicity and sub-solar abundance ratio, without the presence of significant radial gradients. On the other hand, their ages show strong negative gradients and the possible indication that the secondary component is the youngest. We interpret our results in light of recent cosmological simulations and suggest gas accretion along two filaments as the formation mechanism of the stellar counter-rotating components in NGC 4191 (Abridged).Comment: 10 pages, 10 figure. Accepted for publication in Astronomy and Astrophysic