Genetic diversity and production potential of animal food resources

Article Details: Received: 2020-05-21 | Accepted: 2020-06-02 | Available online: 2020-06-30https://doi.org/10.15414/afz.2020.23.02.102-108The submission aims to present results of the five-year research project, oriented on the evaluation of genetic diversity of selected populations of economically important animal species in Slovakia, their sustainable adaptation and production potential in the context of preservation of genetic resources and food safety. Under the supervision of Department of Animal Genetics and Breeding Biology, Faculty of Agrobiology and Food Resources of the Slovak University of Agriculture in Nitra run between 2015- 2019 project called Molecular-genetic diversity and production potential of animal genetic resources in Slovakia (APVV-14-0054). Considering the difficulty and complexity of studied issues was research realized in close collaboration with the University of Natural Resources and Life Sciences Vienna (BOKU) and Zagreb University. Erosion of genetic diversity represents the main threat for food safety of mankind. Individuals of economically important animal species groups accumulate risks and threats of loss of sustainable adaptation as a reaction to the environment due to intense selective breeding. It is therefore important and needed to focus on permanent monitoring and evaluation of diversity of economically important breeds based on the diverse parameter and suitable methods.Keywords: Genetic diversity, economically important breeds, Animal genetic resources, SlovakiaReferencesKADLEČÍK, O., HAZUCHOVÁ, E., MORAVČÍKOVÁ, N. and KUKUČKOVÁ, V. (2017b). Genetic diversity in Slovak spotted breed. AGROFOR, 2(3), 124–131.KADLEČÍK, O., HAZUCHOVÁ, E., PAVLÍK, I. and KASARDA, R. (2016). Genetická diverzita slovenského strakatého a holštajnského dobytka (1. vyd). Nitra: Slovenská poľnohospodárska univerzita.KADLEČÍK, O., MORAVČÍKOVÁ, N. and KASARDA, R. (2017a). Biodiverzita populácií zvierat. 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CSR Reporting as an Important Tool of CSR Communication

AbstractThe article focuses on Corporate Social Responsibility (CSR) and the importance of reports within communication of CSR. 1953 is considered to be a breakthrough year, when it was first referred to the definition of social responsibility. CSR is a trend that appeals to change of business orientation from short-term to long-term goals and from maximum to optimum profit. CSR reports, respectively triple-bottom-line reports have become tool of communication for Corporate Social Responsibility. Those are a voluntary comprehensive reports involving not only economic data, but also information from environmental and social field. These reports tell about the company policy in relationship to the environment, sustainability, or there are directly focused on fulfilling the commitments accepted by the company within the concept of social responsibility. CSR report can help to bring a systematic approach into the management of socially responsible activities, identify future risks and opportunities and thereby contribute to increasing the competitiveness of business and maintain the possibility for long-term business venture. Information is not only for the company, but on the basis of them enterprise can partly to create decision-making process of different types of stakeholders. This article points out how the CSR report is used by businesses and how socially responsible activities through CSR reports are perceived by Slovak customer. We will use secondary information collected from marketing agencies surveys and primary information collected from own survey. We will use these methods: analysis, synthesis and deduction

The Use of Demographic and Psychographic Segmentation to Creating Marketing Strategy of Brand Loyalty

With growing competition, loyal customers have become the key to the company's success. Brand loyalty has been a central structure for marketing for almost a century, yet this research topic is still modern and up to date. The aim of this contribution is to answer the research question of whether there are different segments of customers based on demographic and psychographic aspects that would differ in the level of brand loyalty in the company. In other words, do certain groups of company's customers (according to demographic or psychographic segmentation) have a higher degree of loyalty to the company's brand? To answer the research question, we have identified hypotheses expressing the existence of a statistical dependence between individual segmentation variable and the level of brand loyalty. Based on statistical testing of established hypotheses, we have confirmed the existence of certain company's segments that have a higher degree of loyalty.JEL Codes - M21; M3

The insectivorous sundew (Drosera rotundifolia, L.) might be a novel source of PR genes for biotechnology

The gene pool of insectivorous sundew, Drosera rotundifolia L., was studied to identify and analyse sequences encoding for pathogenesis-related (PR) proteins. The digested genomic DNA was in ¿inverted¿ Southern hybridisation probed to 19 clones for PR genes from different plant sources. From representatives of PR subgroups 1¿5, 8 and 9, genes for glucanases (PR-2), chitinases (PR-3) and thaumatin-like proteins (PR-5) were hybridising. A PCR approach using degenerated primers was chosen to isolate sequences of sundew glucanase gene. Translation of a 500 bp long putative glucanase revealed similarity to catalytic domain of other glucanase amino acid sequences. Despite the peculiarity of this sequence, it contains all conserved amino acid residues important for catalysis. The sequence obtained in this study represents one of the first sequences encoding for nuclear genes in sundews reported, and brings the first evidence for presence of glucanases in sundew. The potential use of this sequence in biotechnology is considered as well

Tentacles of in vitro-grown round-leaf sundew (Drosera rotundifolia L.) show induction of chitinase activity upon mimicking the presence of prey

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Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens.

BackgroundTo determine differentially expressed and spliced RNA transcripts in chronic lymphocytic leukemia specimens a high throughput RNA-sequencing (HTS RNA-seq) analysis was performed.MethodsTen CLL specimens and five normal peripheral blood CD19+ B cells were analyzed by HTS RNA-seq. The library preparation was performed with Illumina TrueSeq RNA kit and analyzed by Illumina HiSeq 2000 sequencing system.ResultsAn average of 48.5 million reads for B cells, and 50.6 million reads for CLL specimens were obtained with 10396 and 10448 assembled transcripts for normal B cells and primary CLL specimens respectively. With the Cuffdiff analysis, 2091 differentially expressed genes (DEG) between B cells and CLL specimens based on FPKM (fragments per kilobase of transcript per million reads and false discovery rate, FDR q < 0.05, fold change >2) were identified. Expression of selected DEGs (n = 32) with up regulated and down regulated expression in CLL from RNA-seq data were also analyzed by qRT-PCR in a test cohort of CLL specimens. Even though there was a variation in fold expression of DEG genes between RNA-seq and qRT-PCR; more than 90 % of analyzed genes were validated by qRT-PCR analysis. Analysis of RNA-seq data for splicing alterations in CLL and B cells was performed by Multivariate Analysis of Transcript Splicing (MATS analysis). Skipped exon was the most frequent splicing alteration in CLL specimens with 128 significant events (P-value <0.05, minimum inclusion level difference >0.1).ConclusionThe RNA-seq analysis of CLL specimens identifies novel DEG and alternatively spliced genes that are potential prognostic markers and therapeutic targets. High level of validation by qRT-PCR for a number of DEG genes supports the accuracy of this analysis. Global comparison of transcriptomes of B cells, IGVH non-mutated CLL (U-CLL) and mutated CLL specimens (M-CLL) with multidimensional scaling analysis was able to segregate CLL and B cell transcriptomes but the M-CLL and U-CLL transcriptomes were indistinguishable. The analysis of HTS RNA-seq data to identify alternative splicing events and other genetic abnormalities specific to CLL is an added advantage of RNA-seq that is not feasible with other genome wide analysis

The Use of Demographic and Psychographic Segmentation to Creating Marketing Strategy of Brand Loyalty

With growing competition, loyal customers have become the key to the company’s success. Brand loyalty has been a central structure for marketing for almost a century, yet this research topic is still modern and up to date. The aim of this contribution is to answer the research question of whether there are different segments of customers based on demographic and psychographic aspects that would differ in the level of brand loyalty in the company. In other words, do certain groups of company’s customers (according to demographic or psychographic segmentation) have a higher degree of loyalty to the company’s brand? To answer the research question, we have identified hypotheses expressing the existence of a statistical dependence between individual segmentation variable and the level of brand loyalty. Based on statistical testing of established hypotheses, we have confirmed the existence of certain company’s segments that have a higher degree of loyalty

Stable transformation of embryogenic tissues of Pinus nigra Arn. using a biolistic method

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