El agua y la reproducción asexual en los hongos ingoldianos.

Sin resume

MARS, the MAGIC Analysis and Reconstruction Software

With the commissioning of the second MAGIC gamma-ray Cherenkov telescope situated close to MAGIC-I, the standard analysis package of the MAGIC collaboration, MARS, has been upgraded in order to perform the stereoscopic reconstruction of the detected atmospheric showers. MARS is a ROOT-based code written in C++, which includes all the necessary algorithms to transform the raw data recorded by the telescopes into information about the physics parameters of the observed targets. An overview of the methods for extracting the basic shower parameters is presented, together with a description of the tools used in the background discrimination and in the estimation of the gamma-ray source spectra.Comment: 4 pages, 0 figures, submitted to the 31st International Cosmic Ray Conference, {\L}odz 200

Ultrafast imaging Raman spectroscopy of large-area samples without stepwise scanning

Step-by-step, time-consuming scanning of the sample is still the state-of-the-art in imaging Raman spectroscopy. Even for a few 100 image points the measurement time may add up to minutes or hours. A radical decrease in measurement time can be achieved by applying multiplex spectrographs coupled to imaging fiber bundles that are successfully used in astronomy. For optimal use of the scarce and expensive observation time at astronomical observatories, special high-performance spectrograph systems were developed. They are designed for recording thousands of spatially resolved spectra of a two-dimensional image field within one single exposure. Transferring this technology to imaging Raman spectroscopy allows a considerably faster acquisition of chemical maps. Currently, an imaging field of up to 1 cm2 can be investigated. For porcine skin the required measurement time is less than 1 min. For this reason, this technique is of particular interest for medical diagnostics, e.g., the identification of potentially cancerous abnormalities of skin tissue

Silencing of the Aspergillopepsin B (pepB) Gene of Aspergillus awamori by Antisense RNA Expression or Protease Removal by Gene Disruption Results in a Large Increase in Thaumatin Production

[EN] Aspergillopepsin B was identified in culture broths of Aspergillus awamori by in situ detection of its proteolytic activity and by immunodetection with anti-aspergillopepsin B antibodies. Severe thaumatin degradation was observed after in vitro treatment of thaumatin with purified aspergillopepsin B. The pepB gene encoding aspergillopepsin B of A. awamori was cloned and characterized. It is located in chromosome IV of A. awamori, as shown by pulsed-field gel electrophoresis, and encodes a protein of 282 amino acids with high similarity to the aspergillopepsin B of Aspergillus niger var. macrosporus. The pepB gene is expressed at high rates as a monocistronic 1.0-kb transcript in media with casein at acidic pH values. An antisense cassette constructed by inserting the pepB gene in the antisense orientation downstream from the gpdA promoter resulted in a good level of antisense mRNA, as shown by reverse transcription-PCR. Partial silencing of the pepB gene by the antisense mRNA resulted in a 31% increase in thaumatin yield. However, significant residual degradation of thaumatin still occurred. To completely remove aspergillopepsin B, the pepB gene was deleted by double crossover. Two of the selected transformants lacked the endogenous pepB gene and did not form aspergillopepsin B. Thaumatin yields increased by between 45% in transformant APB 7/25 and 125% in transformant 7/36 with respect to the parental strain. Reduction of proteolytic degradation by gene silencing with antisense mRNA or total removal of the aspergillopepsin B by directed gene deletion was a very useful method for improving thaumatin production in A. awamori.S

The MAGIC Experiment and Its First Results

With its diameter of 17m, the MAGIC telescope is the largest Cherenkov detector for gamma ray astrophysics. It is sensitive to photons above an energy of 30 GeV. MAGIC started operations in October 2003 and is currently taking data. This report summarizes its main characteristics, its rst results and its potential for physics.Comment: 6 pages, 3 figures, to be published in the Proceedings of the 6th International Symposium ''Frontiers of Fundamental and Computational Physics'' (FFP6), Udine (Italy), Sep. 26-29, 200