2,280 research outputs found
Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization
Author summary The intestinal epithelium is a crucial biological interface, interacting with both commensal and pathogenic microorganisms. It’s lined with heavily glycosylated proteins and glycolipids which can act as both attachment sites and energy sources for intestinal bacteria. Fut2, the enzyme governing epithelial α1,2-fucosylation, has been implicated in the interaction between microbes and intestinal epithelial cells. Salmonella is one of the most important bacterial gastrointestinal pathogens affecting millions of people worldwide. Salmonella possesses fimbrial and non-fimbrial adhesins which can be used to adhere to host cells. Here we show that Salmonella expresses Std fimbriae in the gastrointestinal tract in vivo and exploit Std fimbriae to bind fucosylated structures in the mucus and on the intestinal epithelium. Furthermore, we demonstrate that the Std fimbriae-fucose interaction is necessary for bacterial colonization of the intestine and for triggering intestinal inflammation. These data lend new insights into bacterial adhesion-epithelial interactions which are essential for bacterial pathogenesis and key factors in determining tissue tropism and host susceptibility to infectious disease
Small grid embeddings of 3-polytopes
We introduce an algorithm that embeds a given 3-connected planar graph as a
convex 3-polytope with integer coordinates. The size of the coordinates is
bounded by . If the graph contains a triangle we can
bound the integer coordinates by . If the graph contains a
quadrilateral we can bound the integer coordinates by . The
crucial part of the algorithm is to find a convex plane embedding whose edges
can be weighted such that the sum of the weighted edges, seen as vectors,
cancel at every point. It is well known that this can be guaranteed for the
interior vertices by applying a technique of Tutte. We show how to extend
Tutte's ideas to construct a plane embedding where the weighted vector sums
cancel also on the vertices of the boundary face
Quantum key distribution via quantum encryption
A quantum key distribution protocol based on quantum encryption is presented
in this Brief Report. In this protocol, the previously shared
Einstein-Podolsky-Rosen pairs act as the quantum key to encode and decode the
classical cryptography key. The quantum key is reusable and the eavesdropper
cannot elicit any information from the particle Alice sends to Bob. The concept
of quantum encryption is also discussed.Comment: 4 Pages, No Figure. Final version to appear in PR
High Spatial Resolution Fast-Neutron Imaging Detectors for Pulsed Fast-Neutron Transmission Spectroscopy
Two generations of a novel detector for high-resolution transmission imaging
and spectrometry of fast-neutrons are presented. These devices are based on a
hydrogenous fiber scintillator screen and single- or multiple-gated intensified
camera systems (ICCD). This detector is designed for energy-selective neutron
radiography with nanosecond-pulsed broad-energy (1 - 10 MeV) neutron beams.
Utilizing the Time-of-Flight (TOF) method, such a detector is capable of
simultaneously capturing several images, each at a different neutron energy
(TOF). In addition, a gamma-ray image can also be simultaneously registered,
allowing combined neutron/gamma inspection of objects. This permits combining
the sensitivity of the fast-neutron resonance method to low-Z elements with
that of gamma radiography to high-Z materials.Comment: Also published in JINST:
http://www.iop.org/EJ/abstract/1748-0221/4/05/P0501
Quantum key distribution without alternative measurements
Entanglement swapping between Einstein-Podolsky-Rosen (EPR) pairs can be used
to generate the same sequence of random bits in two remote places. A quantum
key distribution protocol based on this idea is described. The scheme exhibits
the following features. (a) It does not require that Alice and Bob choose
between alternative measurements, therefore improving the rate of generated
bits by transmitted qubit. (b) It allows Alice and Bob to generate a key of
arbitrary length using a single quantum system (three EPR pairs), instead of a
long sequence of them. (c) Detecting Eve requires the comparison of fewer bits.
(d) Entanglement is an essential ingredient. The scheme assumes reliable
measurements of the Bell operator.Comment: REVTeX, 5 pages, 2 figures. Published version with some comment
Detector decoy quantum key distribution
Photon number resolving detectors can enhance the performance of many
practical quantum cryptographic setups. In this paper, we employ a simple
method to estimate the statistics provided by such a photon number resolving
detector using only a threshold detector together with a variable attenuator.
This idea is similar in spirit to that of the decoy state technique, and is
specially suited for those scenarios where only a few parameters of the photon
number statistics of the incoming signals have to be estimated. As an
illustration of the potential applicability of the method in quantum
communication protocols, we use it to prove security of an entanglement based
quantum key distribution scheme with an untrusted source without the need of a
squash model and by solely using this extra idea. In this sense, this detector
decoy method can be seen as a different conceptual approach to adapt a single
photon security proof to its physical, full optical implementation. We show
that in this scenario the legitimate users can now even discard the double
click events from the raw key data without compromising the security of the
scheme, and we present simulations on the performance of the BB84 and the
6-state quantum key distribution protocols.Comment: 27 pages, 7 figure
NAAA-regulated lipid signaling governs the transition from acute to chronic pain
Chronic pain affects 1.5 billion people worldwide but remains woefully undertreated. Understanding the molecular events leading to its emergence is necessary to discover disease-modifying therapies. Here we show that N-acylethanolamine acid amidase (NAAA) is a critical control point in the progression to pain chronicity, which can be effectively targeted by small-molecule therapeutics that inhibit this enzyme. NAAA catalyzes the deactivating hydrolysis of palmitoylethanolamide, a lipid-derived agonist of the transcriptional regulator of cellular metabolism, peroxisome proliferator-activated receptor-α (PPAR-α). Our results show that disabling NAAA in spinal cord during a 72-h time window following peripheral tissue injury halts chronic pain development in male and female mice by triggering a PPAR-α-dependent reprogramming of local core metabolism from aerobic glycolysis, which is transiently enhanced after end-organ damage, to mitochondrial respiration. The results identify NAAA as a crucial control node in the transition to chronic pain and a molecular target for disease-modifying medicines
Does regulating private long-term care facilities lead to better care? a study from Quebec, Canada
Objective. In the province of Quebec, Canada, long-term residential care is provided by 2 types of facilities: publicly-funded accredited facilities and privately-owned facilities in which care is privately financed and delivered. Following evidence that private facilities were delivering inadequate care, the provincial government decided to regulate this industry. We assessed the impact of regulation on care quality by comparing quality assessments made before and after regulation. In both periods, public facilities served as a comparison group.
Design: A cross-sectional study conducted in 2010-2012 that incorporates data collected in 1995-2000.
Settings. Random samples of private and public facilities from 2 regions of Quebec.
Participants. Random samples of disabled residents aged 65 years and over. In total, 451 residents from 145 care settings assessed in 1995-2000 were compared to 329 residents from 102 care settings assessed in 2010-2012.
Intervention. Regulation introduced by the province in 2005, effective February 2007.
Main outcome measure. Quality of care measured with the QUALCARE Scale.
Results. After regulation, fewer small-size facilities were in operation in the private market. Between the 2 study periods, the proportion of residents with severe disabilities decreased in private facilities while it remained over 80% in their public counterparts. Meanwhile, quality of care improved significantly in private facilities, while worsening in their public counterparts, even after controlling for confounding.
Conclusions. The private industry now provides better care to its residents. Improvement in care quality likely results in part from the closure of small homes and change in resident case-mix
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