55 research outputs found
Dynamic Behavior in Enzyme-Polymer Surfactant Hydrogel Films
Dynamic protein-polymer surfactant films are highly hydrophilic and show a soft solid to hydrogel transition upon hydration to produce a swollen hydrogel. An unusual reversible autospreading/self-folding response is observed when the water-saturated films are transferred from water into air
Concomitant differentiation of a population of mouse embryonic stem cells into neuron‐like cells and schwann cell–like cells in a slow‐flow microfluidic device
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135245/1/dvdy24466_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/135245/2/dvdy24466.pd
Exploiting novel valve interstitial cell lines to study calcific aortic valve disease
Calcific aortic valve disease (CAVD) involves progressive valve leaflet thickening and severe calcification, impairing leaflet motion. The in vitro calcification of primary rat, human, porcine and bovine aortic valve interstitial cells (VICs) is commonly employed to investigate CAVD mechanisms. However, to date, no published studies have utilised cell lines to investigate this process. The present study has therefore generated and evaluated the calcification potential of immortalized cell lines derived from sheep and rat VICs. Immortalised sheep (SAVIC) and rat (RAVIC) cell lines were produced by transduction with a recombinant lentivirus encoding the Simian virus (SV40) large and small T antigens (sheep), or large T antigen only (rat), which expressed markers of VICs (vimentin and -smooth muscle actin). Calcification was induced in the presence of calcium (Ca; 2.7 mM) in SAVICs (1.9 fold;
A time course investigation of the statin paradox among valvular interstitial cell phenotypes
Phospholipids accumulation and calcification in cultured primary human aortic valve interstitial cells : new insights revealed by confocal Raman imaging
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