Isolasi dan Karakterisasi Selulase dari Trichoderma Viride dan Rhizopus Spp dengan Substrat Jerami Padi

The objectives of this study were to isolated and produce cellulase from soil molds and to characterize the enzyme. The strains of mold isolated from soil, namely Rhizopus spp and one cultured mold. Trichoderma viride, was used to produce the enzymes. Medium for enzyme production consisted of NH4NO3, KCl, FeSO4.7H2O, MgSO4.7H2O, CuSO4.2H2O and rice straw as a sole source of carbon (pH : 5.45). Culture was done at 28oC for 5,8,11 and 14 days with shaking at 150 rpm. Using this method cellulase production was optimum at 14 days with substrat concentration of 1.5% for T. viride culture. However the optimum production of cellulase for Rhizopus spp culture were three days shorter than culture of T.viride with substrat concentration of 1.5 and 1% respectively. Temperature and pH optimal activity of cellulases were as follow : cellulase from T.viride at temperature 60oC and pH 5 and cellulase from Rhizopus spp at temperature 50oC and pH 5. While temperature and pH stability of cellulases were as follow : cellulase from T. viride at temperature 30 - 80oC and pH 3 - 7 and cellulase from Rhizopus spp at temperature 30 - 80oC and pH 3-6. It was concluded that molds can grow on rice straw as a sole source of carbon produce cellulases

Pengaruh Suhu dan Lama Penyimpanan terhadap Stabilitas Minyak Ikan dan Mikrokapsul Minyak Ikan

Fish oil microcapsules could be stored long longer than fish oil. Storage of the fish oil microcapsules to be more stable and effective to note factors such as temperature and storage time. The purpose of this study was to determine the effect of temperature and storage time on the stability of fish oil (MI) and microencapsulated fish oil (MMI). The research method used was a completely randomized design method factorial 2 x 3 and 3 replications for each MI and MMI. Factor A (storage temperature: refrigerator temperature and room temperature) and factor B (storage time: 15, 30 and 45 days). The variables measured were peroxide, TBA numbers and the total number of oxidation. The results of this study found that the more stable MMI stored in the refrigerator compared to room temperature and the MMI is more stable compared with MI. Temperature and storage time is best for the oxidative stability of microencapsulated fish oil and fish oil are refrigerator temperature and storage time of 45 days

Crude Nutrient and Mineral Composition of Asystasia Gangetica (L) Derived from Different Growing Areas

Asystasia gangetica as a predominant plant species for feeding goat grows widely in various lands in Payakumbuh region. The present study was aimed to determine crude nutrient and mineral composition of the wild plant derived from five different growing areas, i.e. banana and cacao plantations, road sides, idle lands and river banks. The sampling sites were distributed in 3 different subdistricts. Fresh samples which were collected by using a plate meter of 0.5 x 0.5 m2 were weiighed, chopped, dried and then ground in meal form prior to analysis for dry matter (DM), crude ash, crude protein (CP), crude fiber (CF), acid detergent fiber (ADF), cellulose, and minerals of Ca, P, Mg, Cu, Zn, Mn. Data were subjected to analysis of variance (ANOVA) in completely randomixed design of 5x3 consisting of 5 growing areas and 3 subdistricts as replications. Results showed that A. gangetica contained relatively low DM of 9.3-11.9%. Crude ash ranged 16.8-20.9%, CP: 15.5-25.0%, CF: 23.2-28.6 %, ADF: 42.5-46.4%, and cellulose :26.5-29.7%. Macro mineral Ca ranged 18.8-20.5 g, P:11.2-17.4, Mg: 7.5-8.5 g/kg DM. Trace element of Cu ranged 22.0- 29.4 mg, Zn:17.4-22.6 mg, Mn: 36.2-41.4 mg/kg DM. A. gangetica grown in the banana plantation areas showed the highest crude protein content, while the highest fiber content was found by A. gangetica collected from the cocoa plantation. A. gangetica grown in river banks tended to have the higher mineral content of Ca, P, Mg, and Cu

PENGEMBANGAN USAHA ITIK PETELUR “REGRA HATCHERY”DI LUBUK TAROK KABUPATEN SIJUNJUNG

Pada tahun 2021 ini tim abdimas kembali turun untuk membantu pengembangan usaha  RegRa Hatchery di Lubuk Tarok Sijunjung dengan menyediakan itik petelur bibit dari strain itik ratu. Mitra tersebut sangat menunggunya karena telah membangun kandang untuk itik petelur bibit tersebut dalam kegiatan pengabdian tahun 2020. Upaya yang dilakukan tim abdimas untuk mengembangkan usaha tersebut yaitu dengan memberikan bantuan berupa itik petelur bibit dan beberapa bahan penyusun ransum itik tersebut, sehingga ke depannya dapat menghasilkan telur bibit sendiri dengan tidak mendatangkan telur bibit tetas dari tempat lain seperti yang dilakukan sebelumnya. Metode yang dilakukan dalam pengabdian ini adalah memberikan masukan dan demonstrasi dalam manajemen pemeliharaan itik petelur bibit, manajemen dalam pengadukan dan penyusunan ransum sesuai dengan formulasi ransum yang telah disusun oleh tim abdimas, memperkenalkan budidaya maggot BSF sebagai bahan pakan alternatif dalam ransum.  Hasil dari pengabdian tersebut adalah mitra sangat antusias dalam melakukan semua masukan dan saran yang diberikan oleh tim abdimas, mitra mengajukan beberapa pertanyaan-pertanyaan tentang manajemen beternak itik tersebut. Kesimpulan dari pengabdian ini adalah mitra mampu menjalankan budidaya itik petelur bibit dengan pendampingan dari tim abdimas. Kata kunci: Itik petelur bibit, regra hatchery, Lubuk Tarok, maggot BSF, formulasi ransum ABSTRACT In 2021, the community service team returned to assist the development of the "RegRa Hatchery" business in Lubuk Tarok Sijunjung by providing breed layer ducks from the “ratu  duck strain. The partner was really waiting for him because he had built a cage for the breed layer ducks in the service activity in 2020. The efforts made by the community service team to develop the business were by providing assistance in the form of breed layer ducks and some of the ingredients for the duck diet so that in the future, they can produce eggs—own seeds by not bringing in hatching eggs from other places, as was done before. The method used in this service is to provide input and demonstrations in the management of laying ducks, management in mixing and compiling rations according to the ration formulation that has been prepared by the community service team introducing BSF maggot cultivation as an alternative feed ingredient in rations. The result of this service is that partners are very enthusiastic in carrying out all the input and suggestions given by the community service team; partners ask several questions about the management of raising ducks. The conclusion of this service is that partners are able to carry out breed layer duck cultivation with assistance from the community service team. Keywords: breed layer ducks, "Regra hatchery", Lubuk  Tarok, BSF maggot, ration formulatio

Pengaruh Pemberian Mikrokapsul Minyak Ikan dalam Ransum Puyuh terhadap Performa Produksi

An experiment was conducted to determine the effect of feeding fish oil microcapsule on quail performances.  200 quail layers were randomly allocated into 20 pens.  A randomized completely design was used in this study with 0, 2%, 4% and 6% fish oil microcapsule in diet.  Variable measured were feed consumption, egg production, egg weight, mass of egg and feed conversion. Data were analized by CRD and Duncan Multiple Range Test (DMRT).  Results of the experiment indicated that the inclusion of fish oil microencapsule in laying quails diet did not affect the production performances (feed consumption, egg production, egg weight, mass of egg and feed conversion)

Pengaruh Pemberian Produk Fermentasi Rumput Laut (Turbinaria murayana) dalam Ransum terhadap Performa Produksi Puyuh Petelur (Coturnix coturnix japonica)

Tujuan penelitian untuk mengetahui pengaruh pemberian rumput laut (Turbinaria murayana) yang telah difermentasi menggunakan Mikroorganisme Lokal (MOL) dari limbah buah dalam ransum puyuh petelur (Coturnix-coturnix japonica). Penelitian menggunakan puyuh petelur umur 36 minggu dengan rata-rata produksi telur 65% sebanyak 200 ekor dan rumput laut (Turbinaria murayana) yang telah difermentasi menggunakan MOL buah serta bahan-bahan penyusun ransum lainnya seperti jagung giling, dedak halus, tepung ikan, bungkil kedelai, corn glutean meal (CGM), tepung batu dan top mix. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) yang terdiri dari 5 perlakuan dan 4 kali ulangan. Perlakuan berupa level pemberian Turbinaria murayana fermentasi MOL buah berbeda dalam ransum (0, 5, 10, 15, dan 20%). Parameter yang diamati adalah konsumsi ransum, produksi telur harian, berat telur, massa telur, dan konversi ransum. Hasil penelitian menunjukkan pengaruh berbeda sangat nyata (P≤0,01) terhadap konsumsi ransum, massa telur, konversi ransum dan produksi telur harian, serta berbeda tidak nyata (P≥0,05) terhadap berat telur. Turbinaria murayana fermentasi dapat dijadikan sebagai bahan pakan untuk menggantikan penggunaan dedak halus dan menurunkan penggunaan jagung giling, tepung ikan dan bungkil kedelai dalam ransum pada level pemberian 20% dengan rata-rata konsumsi ransum 19,51 gram/ekor/hari, produksi telur harian 57,19%, berat telur 9,42 gram/butir, massa telur 4,31 gram/butir, dan konversi ransum 4,53

PRODUKSI ENZIM SELULASE TERMOSTABIL DARI BAKTERI NG2 MENGGUNAKAN BERBAGAI SUMBER SELULOSA ASAL LIMBAH PERTANIAN DAN PERKEBUNAN (Production of Thermostable Cellulase Enzyme by NG2 Bacteria Using Various Cellulose Sources from the Agriculture Waste)

This experiment aimed to determine the best cellulose sources from the agricultural by product on the production of the thermostable cellulase enzymes by thermophilic bacteria (NG2) and also to find out the best concentration level of the selected sources of cellulose. The experiment was conducted in two stages using a completely randomized design (CRD). In phase 1, four cellulose sources were used, namely A: corn cobs, B: rice straw, C: coconut fiber, and D: oil palm trunk, in which the replication for each source was five. In phase 2,  the best sources of cellulose from the phase 1 was used at four different levels of concentration, i.e., A: 2%, B: 3%, C: 4%, and D: 5%, in which replication for each concentration was five. The result of phase 1 showed that the corn cobs was the best cellulose source  in producing cellulase enzyme. This was indicated by the activity of 0.329 U/ml, the enzyme protein of 0.0328 mg/ml, and the specific activity of 10.165 U/mg, which were significantly higher (P<0.01) than those of other cellulose sources. The results of phase 2 showed that the concentration of 4% (w/v) had the highest (P<0.01) specific activity of 44.002 U/mg  compared with those of the other concentrations. In conclusion, the highest production of cellulase enzyme using thermophilic bacteria (NG2) was obtained at the concentration of 4% (w/v) using the corn cobs as the cellulose source

PENGARUH LAMA FERMENTASI DAN DOSIS INOKULUM Bacillus amyloliquefaciens TERHADAP KANDUNGAN NUTRISI DAUN PAITAN (Tithonia diversifolia)

Penelitian ini bertujuan untuk mengetahui interaksi antara lama fermentasi dengan dosis inokulum Bacillus amyloliquefaciens terhadap kandungan nutrisi daun paitan (Tithonia diversifolia). Metode yang digunakan adalah metode eksperimen dengan rancangan acak lengkap (RAL) faktorial 3 × 3 dengan 3 ulangan. Faktor A (dosis inokulum) terdiri dari A1 = 1%, A2 = 2% dan A3 = 3%. Faktor B (lama fermentasi) terdiri dari B1 = 1 hari, B2 = hari dan B3 = 3 hari. Peubah yang diamati adalah kandungan bahan kering (%), serat kasar (%), dan protein kasar (%). Hasil penelitian menunjukkan bahwa tidak ada interaksi antara dosis inokulum dan lama fermentasi terhadap semua parameter yang diamati akan tetapi dosis inokulum dan lama fermentasi berpengaruh terhadap kandungan protein kasar dan serat kasar daun paitan. Kesimpulan dari penelitian ini adalah lama fermentasi 1 hari dengan dosis inokulum 3% menghasilkan kandungan bahan kering 46,73%, protein kasar 26,40%, dan serat kasar 7,88%. Kata kunci: Bacillus amyloliquefaciens, daun paitan, dosis inokulum, kandungan nutrisi, lama fermentas

Performa Ayam KUB Umur 6 Sampai 12 Minggu yang diberi Maggot BSF (Black Soldier Fly) dalam Ransum

Penelitian ini bertujuan untuk mengetahui performa ayam KUB umur 6 sampai 12 minggu yang diberi maggot BSF (Black Soldier Fly) dalam ransum. Ayam KUB umur 6 minggu digunakan dalam penelitian ini sebanyak 100 ekor. Pemberian perlakuan berupa tepung maggot dalam ransum dilakukan sampai ayam umur 12 minggu. Rancangan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan lima perlakuan dan empat ulangan. Perlakuan yang digunakan adalah P1 (0% maggot), P2 (7% maggot), P3 (14% maggot), P4 (21% maggot), dan P5 (28% maggot). Ransum disusun dengan iso protein dan energi yaitu protein 17,5% dan energi 2800kkal/kg. Parameter performa yang diamati yaitu konsumsi ransum, pertambahan bobot badan, dan konversi ransum. Dari penelitian yang dilakuakan menunjukkan hasil bahwa pemberian maggot BSF dalam ransum berpengaruh nyata (P0,05) terhadap konsumsi dan konversi ransum. Penelitian ini dapat disimpulkan bahwa pemberian tepung maggot BSF sebanyak 14% dalam ransum dapat memberikan performa yang baik pada ayam KUB

LAPORAN PENGABDIAN KEPADA MASYARAKAT NNR & TIP 2020

LAPORAN PENGABDIAN KEPADA MASYARAKAT PADA GABUNGAN PENGUSAHA TERNAK UNGGAS PETELUR KABUPATEN PADANG PARIAMAN Kampung Rao, Kecamatan V koto, Kabupaten Padang Pariaman