The rare case of positive FDG-positron emission tomography for giant cavernous hemangioma of the liver

Hemangioma is the most common benign liver tumor and the second most common liver tumor after metastases. Large hemangiomas are often heterogeneous. When they exceed 4 cm in diameter, they are termed giant hemangiomas. These giant hemangiomas often present heterogeneous patterns. These heterogeneous appearances are shown because of intratumoral changes due to several degenerative phenomena. PET/CT is reported to be useful for the differentiation of benign from malignant liver lesions. We report the case of a large hepatic hemangioma characterized by high FDG uptake

Mind the gap: IR and the challenge of international politics

The discipline of International Relations (IR) for a long time of its history has developed in the form of Great Debates that involved competing paradigms and schools. More recently, it has been described as a cacophony of voices unable to communicate among themselves, but also incapable to provide keys to understand an ever more complex reality. This collection aims at evaluating the heuristic value of a selection of traditional paradigmsrealism and liberalism), schools (constructivism), and subdisciplines (security studies and international political economy) so as to assess the challenges before IR theory today and the ability of the discipline to provide tools to make the changed world still intelligible

Interleukin-34 promotes tumorigenic signals for colon cancer cells

Colorectal carcinoma (CRC) is one of the most common forms of malignancy in the Western world. Accumulating evidence indicates that colon carcinogenesis is tightly controlled by tumour-associated immune cells and stromal cells, which can either stimulate or suppress CRC cell growth and survival, mainly via the production of cytokines. Interleukin-34 (IL-34), a cytokine known to regulate mainly monocyte/macrophage survival and function, is highly produced within the CRC microenvironment by several cell types, including cancer cells, tumour-associated macrophages (TAMs) and cancer-associated fibroblasts (CAFs), and regulates the pro-tumoural functions of such cells. In this article, we summarize the available data supporting the multiple effects of IL-34 in human CRC

Prioritising CAP intervention needs: An improved cumulative voting approach

The process to define the 2023–2027 Common Agricultural Policy (CAP) is underway. The implementation model governing the process requires each EU Member State to design a National Strategic Plan to deliver operational actions exploiting the synergies under the two pillars of the policy. Each Plan must be built from an evidence-based needs assessment that undergoes rigorous prioritisation and planning to create comprehensive, integrated, and achievable interventions. In Italy, the success of this planning process requires all interested stakeholders to generate options for the regional authorities who plan, manage, and legislate agricultural activities. This research proposes a decision-making technique, based on the cumulative voting approach, that can be used effectively when multiple persons from different backgrounds and perspectives are engaged in problem-solving and needs prioritisation. The results indicate that the model can be applied both theoretically and practically to prioritise Strategic Plan needs that involve national and regional authorities. Validation of the model allows it to be used in the next consultative processes and for expansion to socioeconomic stakeholders

Parasite prevalence in a village in Burkina Faso: the contribution of new techniques.

Parasites are a major public health problem in developing countries. A coproparasitological and immunoparasitological study was conducted in Burkina Faso, in the rural village of Touguri, in November and December 2011. The coproparasitologic analysis was conducted in the pediatric population and seroprevalence surveys were conducted in the adult population to research intestinal, blood, and helminth parasites.Methodology: The coproparasitologic study was performed on stool samples using two diagnostic methods –standard microscopy and the FLOTAC technique. The total of 49 stool samples analyzed were obtained from children between two months and eleven years of age. The serology study was carried out to evaluate the prevalence of P. falciparum, Echinococcusspp., Tenia solium, and A. lumbricoidesusing different immunological techniques such as ELISA and Western Blot techniques. The study population included 85 adult patientsbetween 15 and 70 years of age.Results: Results of coproparasitological analyses showed Hymenolepis nanaas the only helminth found, in 28.6% of the total number of patients. Results of serological evaluation revealed a practically null prevalence of Echinococcus, Taenia solium,andAscaris lumbricoides, and a 77.64% prevalence of Plasmodium falciparum.Conclusions: Despite the small number (especially in terms of coprological samples) of individuals examined, this study showed that theparasite prevalence in a rural area of Burkina Faso has a significant impact in the general population, particularly in children. Another finding was that FLOTAC had a higher sensitivity than the widely used ethyl ether-based concentration technique for coprological sample analysis

Comparison of ground‐based and Total Ozone Mapping Spectrometer erythemal UV doses at the island of Lampedusa in the period 1998–2003: Role of tropospheric aerosols

The Total Ozone Mapping Spectrometer (TOMS) has the longest time series of globally distributed estimates of UV irradiance at the Earth's surface. The proper interpretation of TOMS estimated irradiances relies on well‐calibrated and well‐maintained spectrometers at the Earth's surface. In this study, daily erythemal irradiances measured by a Brewer spectrophotometer at the island of Lampedusa (35.5°N, 12.6°E), in the Mediterranean are compared with TOMS observations in the period January 1998 to August 2003. The comparison, also because of the peculiar conditions at Lampedusa, a very good site for ground‐based validation of satellite observations, allows us to recognize how the space‐borne observations are influenced by the presence of atmospheric aerosols. Two TOMS data sets, derived applying different algorithms to retrieve ozone and UV irradiance from the backscattered radiance, are used in this study: Version 7 (V7) and the recently developed version 8 (V8), which uses new climatologies for ozone and temperature profiles and accounts for the attenuation by tropospheric aerosols through the aerosol index (AI). As shown in previous studies performed with V7 TOMS data, satellite‐derived erythemal doses systematically overestimate ground‐based measurements, mainly because of uncorrected absorption by aerosols in the troposphere. The bias between the TOMS and Brewer doses for all‐sky conditions is (9.4 ± 19.8)% for V7 and (7.3 ± 20.0)% for V8 and decreases to (5.6 ± 8.0)% for V7 and (3.4 ± 8.4)% for V8 for the cloud‐free cases. The large standard deviations for all‐sky conditions are due to nonhomogeneity in the cloud cover within the sensor field of view, while those for cloud‐free days are caused by the large aerosol variability occurring at Lampedusa. The biases for cloud‐free days have been related to differences in the TOMS AI UV attenuation algorithm and to the aerosol optical depth (AOD) at 415.6 nm measured with a Sun photometer at Lampedusa since 2001. The mean bias between the V7 TOMS and Brewer doses progressively increases with AI and AOD at 415.6 nm, from ± 3% for low AI and AOD up to 21% for 1.5 ≤ AI < 2.5 and 0.5 ≤ AOD < 0.6. The bias calculated with V8 data set varies between +6% for 0 ≤ AI < 1 and about −8% for 4 ≤ AI < 5, well within the respective uncertainties of the Brewer and TOMS measurements. TOMS V8 data show a smaller dependency on the aerosol absorption, indicating that the implemented corrections produce more reliable estimated doses. For very low aerosol loading (AOD at 415.6 nm below 0.2), the TOMS‐to‐Brewer erythemal dose ratio, both for V7 and V8, is approximately 1, indicating that the radiometric calibration of the Brewer instrument is consistent with the TOMS estimated irradiances from derived ozone and Rayleigh scattering attenuation

Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: their emerging role in tumor heterogeneity

The goal of this study was to understand if exosomes derived from high-metastatic cells may influence the behavior of less aggressive cancer cells and the properties of the endothelium. We found that metastatic colon cancer cells are able to transfer their amoeboid phenotype to isogenic primary cancer cells through exosomes, and that this morphological transition is associated with the acquisition of a more aggressive behavior. Moreover, exosomes from the metastatic line (SW620Exos) exhibited higher ability to cause endothelial hyperpermeability than exosomes from the non metastatic line (SW480Exos). SWATH-based quantitative proteomic analysis highlighted that SW620Exos are significantly enriched in cytoskeletal-associated proteins including proteins activating the RhoA/ROCK pathway, known to induce amoeboid properties and destabilization of endothelial junctions. In particular, thrombin was identified as a key mediator of the effects induced by SW620Exos in target cells, in which we also found a significant increase of RhoA activity. Overall, our results demonstrate that in a heterogeneous context exosomes released by aggressive sub-clones can contribute to accelerate tumor progression by spreading malignant properties that affect both the tumor cell plasticity and the endothelial cell behavior

Tailoring CD19xCD3-DART exposure enhances T-cells to eradication of B-cell neoplasms.

Many patients with B-cell malignancies can be successfully treated, although tumor eradication is rarely achieved. T-cell-directed killing of tumor cells using engineered T-cells or bispecific antibodies is a promising approach for the treatment of hematologic malignancies. We investigated the efficacy of CD19xCD3 DART bispecific antibody in a broad panel of human primary B-cell malignancies. The CD19xCD3 DART identified 2 distinct subsets of patients, in which the neoplastic lymphocytes were eliminated with rapid or slow kinetics. Delayed responses were always overcome by a prolonged or repeated DART exposure. Both CD4 and CD8 effector cytotoxic cells were generated, and DART-mediated killing of CD4+ cells into cytotoxic effectors required the presence of CD8+ cells. Serial exposures to DART led to the exponential expansion of CD4 + and CD8 + cells and to the sequential ablation of neoplastic cells in absence of a PD-L1-mediated exhaustion. Lastly, patient-derived neoplastic B-cells (B-Acute Lymphoblast Leukemia and Diffuse Large B Cell Lymphoma) could be proficiently eradicated in a xenograft mouse model by DART-armed cytokine induced killer (CIK) cells. Collectively, patient tailored DART exposures can result in the effective elimination of CD19 positive leukemia and B-cell lymphoma and the association of bispecific antibodies with unmatched CIK cells represents an effective modality for the treatment of CD19 positive leukemia/lymphoma

Intestinal Taxa Abundance and Diversity in Inflammatory Bowel Disease Patients: An Analysis including Covariates and Confounders

Intestinal dysbiosis has been widely documented in inflammatory bowel diseases (IBDs) and is thought to influence the onset and perpetuation of gut inflammation. However, it remains unclear whether such bacterial changes rely in part on the modification of an IBD-associated lifestyle (e.g., smoking and physical activity) and diet (e.g., rich in dairy products, cereals, meat and vegetables). In this study, we investigated the impact of these habits, which we defined as confounders and covariates, on the modulation of intestinal taxa abundance and diversity in IBD patients. 16S rRNA gene sequence analysis was performed using genomic DNA extracted from the faecal samples of 52 patients with Crohn's disease (CD) and 58 with ulcerative colitis (UC), which are the two main types of IBD, as well as 42 healthy controls (HC). A reduced microbial diversity was documented in the IBD patients compared with the HC. Moreover, we identified specific confounders and covariates that influenced the association between some bacterial taxa and disease extent (in UC patients) or behaviour (in CD patients) compared with the HC. In particular, a PERMANOVA stepwise regression identified the variables "age", "eat yogurt at least four days per week" and "eat dairy products at least 4 days per week" as covariates when comparing the HC and patients affected by ulcerative proctitis (E1), left-sided UC (distal UC) (E2) and extensive UC (pancolitis) (E3). Instead, the variables "age", "gender", "eat meat at least four days per week" and "eat bread at least 4 days per week" were considered as covariates when comparing the HC with the CD patients affected by non-stricturing, non-penetrating (B1), stricturing (B2) and penetrating (B3) diseases. Considering such variables, our analysis indicated that the UC extent differentially modulated the abundance of the Bifidobacteriaceae, Rikenellaceae, Christensenellaceae, Marinifilaceae, Desulfovibrionaceae, Lactobacillaceae, Streptococcaceae and Peptostreptococcaceae families, while the CD behaviour influenced the abundance of Christensenellaceae, Marinifilaceae, Rikenellaceae, Ruminococcaceae, Barnesiellaceae and Coriobacteriaceae families. In conclusion, our study indicated that some covariates and confounders related to an IBD-associated lifestyle and dietary habits influenced the intestinal taxa diversity and relative abundance in the CD and UC patients compared with the HC. Indeed, such variables should be identified and excluded from the analysis to characterize the bacterial families whose abundance is directly modulated by IBD status, as well as disease extent or behaviour

miR-21-mediated regulation of 15-hydroxyprostaglandin dehydrogenase in colon cancer

This work is licensed under a Creative Commons Attribution 4.0 International License.Elevated prostaglandin E2 (PGE2) levels are observed in colorectal cancer (CRC) patients, and this increase is associated with poor prognosis. Increased synthesis of PGE2 in CRC has been shown to occur through COX-2-dependent mechanisms; however, loss of the PGE2-catabolizing enzyme, 15-hydroxyprostaglandin dehydrogenase (15-PGDH, HPGD), in colonic tumors contributes to increased prostaglandin levels and poor patient survival. While loss of 15-PGDH can occur through transcriptional mechanisms, we demonstrate that 15-PGDH can be additionally regulated by a miRNA-mediated mechanism. We show that 15-PGDH and miR-21 are inversely correlated in CRC patients, with increased miR-21 levels associating with low 15-PGDH expression. 15-PGDH can be directly regulated by miR-21 through distinct sites in its 3′ untranslated region (3′UTR), and miR-21 expression in CRC cells attenuates 15-PGDH and promotes increased PGE2 levels. Additionally, epithelial growth factor (EGF) signaling suppresses 15-PGDH expression while simultaneously enhancing miR-21 levels. miR-21 inhibition represses CRC cell proliferation, which is enhanced with EGF receptor (EGFR) inhibition. These findings present a novel regulatory mechanism of 15-PGDH by miR-21, and how dysregulated expression of miR-21 may contribute to loss of 15-PGDH expression and promote CRC progression via increased accumulation of PGE2.NIH R01 CA134609NIH R01 AR069044NIH/NCI Cancer Center Support Grant (P30 CA168524)New Jersey Commission on Cancer ResearchAmerican Heart Association (15GRNT23240019