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In vivo evaluation of edge-loading in metal-on-metal hip resurfacing patients with pseudotumours
Objectives: Pseudotumours (abnormal peri-prosthetic soft-tissue reactions) following metal-on-metal hip resurfacing arthroplasty (MoMHRA) have been associated with elevated metal ion levels, suggesting that excessive wear may occur due to edge-loading of these MoM implants. This study aimed to quantify in vivo edge-loading in MoMHRA patients with and without pseudotumours during functional activities. Methods: The duration and magnitude of edge-loading in vivo was quantified during functional activities by combining the dynamic hip joint segment contact force calculated from the three-dimensional (3D) motion analysis system with the 3D reconstruction of orientation of the acetabular component and each patient’s specific hip joint centre, based on CT scans. Results: Edge-loading in the hips with pseudotumours occurred with a four-fold increase in duration and magnitude of force compared with the hips without pseudotumours (p = 0.02). Conclusions: The study provides the first in vivo evidence to support that edge-loading is an important mechanism that leads to localised excessive wear (edge-wear), with subsequent elevation of metal ion levels in MoMHRA patients with pseudotumours
Lyman-alpha absorption around nearby galaxies
We have used STIS aboard HST to search for Lyman-alpha (Lya) absorption lines
in the outer regions of eight nearby galaxies using background QSOs and AGN as
probes. Lya lines are detected within a few hundred km/s of the systemic
velocity of the galaxy in all cases. We conclude that a background
line-of-sight which passes within 26-200 h-1 kpc of a foreground galaxy is
likely to intercept low column density neutral hydrogen with log N(HI) >~ 13.0.
The ubiquity of detections implies a covering factor of ~ 100% for low N(HI)
gas around galaxies within 200 h-1 kpc. We discuss the difficulty in trying to
associate individual absorption components with the selected galaxies and their
neighbors, but show that by degrading our STIS data to lower resolutions, we
are able to reproduce the anti-correlation of Lya equivalent width and impact
parameter found at higher redshift. We also show that the equivalent width and
column density of Lya complexes (when individual components are summed over ~
1000 km/s) correlate well with a simple estimate of the volume density of
galaxies brighter than M(B) = -17.5 at the same redshift as a Lya complex. We
do not reject the hypothesis that the selected galaxies are directly
responsible for the observed Lya lines, but our analysis indicates that
absorption by clumpy intragroup gas is an equally likely explanation. (Abriged)Comment: Accepted for publication in Nov 20, 2002 issue of ApJ. Paper with all
figures can be found at http://www.astro.princeton.edu/~dvb/lyapaper.ps
(preferable). Minor typos fixe
Weighted network modules
The inclusion of link weights into the analysis of network properties allows
a deeper insight into the (often overlapping) modular structure of real-world
webs. We introduce a clustering algorithm (CPMw, Clique Percolation Method with
weights) for weighted networks based on the concept of percolating k-cliques
with high enough intensity. The algorithm allows overlaps between the modules.
First, we give detailed analytical and numerical results about the critical
point of weighted k-clique percolation on (weighted) Erdos-Renyi graphs. Then,
for a scientist collaboration web and a stock correlation graph we compute
three-link weight correlations and with the CPMw the weighted modules. After
reshuffling link weights in both networks and computing the same quantities for
the randomised control graphs as well, we show that groups of 3 or more strong
links prefer to cluster together in both original graphs.Comment: 19 pages, 7 figure
OVERCOMING BIOFILM MEDIATED RESPIRATORY INFECTIONS THROUGH EXPLOITATION OF PATHOGEN AND HOST-DIRECTED NOVEL PEPTIDES
Whole genome sequencing-based mapping and candidate identification of mutations from fixed zebrafish tissue
As forward genetic screens in zebrafish become more common, the number of mutants that cannot be identified by gross morphology or through transgenic approaches, such as many nervous system defects, has also increased. Screening for these difficult-to-visualize phenotypes demands techniques such as whole-mount in situ hybridization (WISH) or antibody staining, which require tissue fixation. To date, fixed tissue has not been amenable for generating libraries for whole genome sequencing (WGS). Here, we describe a method for using genomic DNA from fixed tissue and a bioinformatics suite for WGS-based mapping of zebrafish mutants. We tested our protocol using two known zebrafish mutant alleles, gpr126st49 and egr2bfh227, both of which cause myelin defects. As further proof of concept we mapped a novel mutation, stl64, identified in a zebrafish WISH screen for myelination defects. We linked stl64 to chromosome 1 and identified a candidate nonsense mutation in the F-box and WD repeat domain containing 7 (fbxw7) gene. Importantly, stl64 mutants phenocopy previously described fbxw7vu56 mutants, and knockdown of fbxw7 in wild-type animals produced similar defects, demonstrating that stl64 disrupts fbxw7. Together, these data show that our mapping protocol can map and identify causative lesions in mutant screens that require tissue fixation for phenotypic analysis
The ins and outs of participation in a weather information system
In this paper our aim is to show even though access to technology, information or data holds the potential for improved participation, participation is wired into a larger network of actors, artefacts and information practices. We draw on a case study of a weather information system developed and implemented by a non-profit organisation to both describe the configuration of participation, but also critically assess inclusion and exclusion. We present a set of four questions - a basic, practical toolkit - by which we together with the organisation made sense of and evaluated participation in the system
Neon and Sulfur Abundances of Planetary Nebulae in the Magellanic Clouds
The chemical abundances of neon and sulfur for 25 planetary nebulae (PNe) in
the Magellanic Clouds are presented. These abundances have been derived using
mainly infrared data from the Spitzer Space Telescope. The implications for the
chemical evolution of these elements are discussed. A comparison with similarly
obtained abundances of Galactic PNe and HII regions and Magellanic Clouds HII
regions is also given. The average neon abundances are 6.0x10(-5) and
2.7x10(-5) for the PNe in the Large and Small Magellanic Clouds respectively.
These are ~1/3 and 1/6 of the average abundances of Galactic planetary nebulae
to which we compare. The average sulfur abundances for the LMC and SMC are
respectively 2.7x10(-6) and 1.0x10(-6). The Ne/S ratio (23.5) is on average
higher than the ratio found in Galactic PNe (16) but the range of values in
both data sets is similar for most of the objects. The neon abundances found in
PNe and HII regions agree with each other. It is possible that a few (3-4) of
the PNe in the sample have experienced some neon enrichment, but for two of
these objects the high Ne/S ratio can be explained by their very low sulfur
abundances. The neon and sulfur abundances derived in this paper are also
compared to previously published abundances using optical data and
photo-ionization models.Comment: 13 pages, 4 tables, 5 figures. Accepted for publication in Ap
Human Amniocytes Are Receptive to Chemically Induced Reprogramming to Pluripotency
Restoring pluripotency using chemical compounds alone would be a major step forward in developing clinical-grade pluripotent stem cells, but this has not yet been reported in human cells. We previously demonstrated that VPA_ AFS cells, human amniocytes cultivated with valproic acid (VPA) acquired functional pluripotency while remaining distinct from human embryonic stem cells (hESCs), questioning the relationship between the modulation of cell fate and molecular regulation of the pluripotency network. Here, we used single-cell analysis and functional assays to reveal that VPA treatment resulted in a homogeneous population of self-renewing non-transformed cells that fulfill the hallmarks of pluripotency, i.e., a short G1 phase, a dependence on glycolytic metabolism, expression of epigenetic modifications on histones 3 and 4, and reactivation of endogenous OCT4 and downstream targets at a lower level than that observed in hESCs. Mechanistic insights into the process of VPA-induced reprogramming revealed that it was dependent on OCT4 promoter activation, which was achieved independently of the PI3K (phosphatidylinositol 3-kinase)/ AKT/ mTOR (mammalian target of rapamycin) pathway or GSK3 beta inhibition but was concomitant with the presence of acetylated histones H3K9 and H3K56, which promote pluripotency. Our data identify, for the first time, the pluripotent transcriptional and molecular signature and metabolic status of human chemically induced pluripotent stem cells
The Low- and Intermediate-Mass Stellar Population in the Small Magellanic Cloud: The Central Stars of Planetary Nebulae
We present a study on the central stars (CSs) of Planetary Nebulae (PNe)
observed in the Small Magellanic Cloud (SMC) with the Space Telescope Imaging
Spectrograph instrument on-board the HST. The stellar magnitudes have been
measured using broad-band photometry, and Zanstra analysis of the nebulae
provided the stellar temperatures. From the location of the CSs on the HR
diagram, and by comparing the observed CSs with current models of stellar
evolution, we infer the CSs masses. We examine closely the possibility of light
contamination in the bandpass from an unrecognized stellar companion, and we
establish strong constraints on the existence and nature of any binary
companion. We find an average mass of 0.63 Msun, which is similar to the mass
obtained for a sample of CSs in the LMC (0.65 Msun). However, the SMC and LMC
CS mass distributions differ slightly, the SMC sample lacking an
intermediate-mass stellar population (0.65 to 0.75 Msun). We discuss the
significance and possible reasons for the difference between the two mass
distributions. In particular, we consider the differences in the star formation
history between the clouds and the mass-loss rate dependence on metallicity.Comment: 30 pages, 6 figures, 5 tables. To be published in ApJ (October 20
Interleukin 3 stimulates proliferation via protein kinase C activation without increasing inositol lipid turnover.
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