A Herschel/HIFI Legacy Survey of HF and H2O in the Galaxy: Probing Diffuse Molecular Cloud Chemistry

We combine Herschel observations of a total of 12 sources to construct the most uniform survey of HF and H2O in our Galactic disk. Both molecules are detected in absorption along all sight lines. The high spectral resolution of the Heterodyne Instrument for the Far-Infrared (HIFI) allows us to compare the HF and H2O distributions in 47 diffuse cloud components sampling the disk. We find that the HF and H2O velocity distributions follow each other almost perfectly and establish that HF and H2O probe the same gas-phase volume. Our observations corroborate theoretical predictions that HF is a sensitive tracer of H2 in diffuse clouds, down to molecular fractions of only a few percent. Using HF to trace H2 in our sample, we find that the N(H2O)-to-N(HF) ratio shows a narrow distribution with a median value of 1.51. Our results further suggest that H2O might be used as a tracer of H2 -within a factor 2.5- in the diffuse interstellar medium. We show that the measured factor of ~2.5 variation around the median is driven by true local variations in the H2O abundance relative to H2 throughout the disk. The latter variability allows us to test our theoretical understanding of the chemistry of oxygen-bearing molecules in the diffuse gas. We show that both gas-phase and grain-surface chemistry are required to reproduce our H2O observations. This survey thus confirms that grain surface reactions can play a significant role in the chemistry occurring in the diffuse interstellar medium n_H < 1000 cm^-3.Comment: 53 pages; 12 figures, accepted for publication in ApJ main journa

Interstellar CH absorption in the diffuse interstellar medium along the sight-lines to G10.6–0.4 (W31C), W49N, and W51

We report the detection of the ground state N, J = 1, 3/2 → 1, 1/2 doublet of the methylidyne radical CH at ~532 GHz and ~536 GHz with the Herschel/HIFI instrument along the sight-line to the massive star-forming regions G10.6–0.4 (W31C), W49N, and W51. While the molecular cores associated with these massive star-forming regions show emission lines, clouds in the diffuse interstellar medium are detected in absorption against the strong submillimeter background. The combination of hyperfine structure with emission and absorption results in complex profiles, with overlap of the different hyperfine components. The opacities of most of the CH absorption features are linearly correlated with those of CCH, CN, and HCO^+ in the same velocity intervals. In specific narrow velocity intervals, the opacities of CN and HCO^+ deviate from the mean trends, giving rise to more opaque absorption features. We propose that CCH can be used as another tracer of the molecular gas in the absence of better tracers, with [CCH]/[H_2] ~3.2 ± 1.1 × 10^(−8). The observed [CN]/[CH], [CCH]/[CH] abundance ratios suggest that the bulk of the diffuse matter along the lines of sight has gas densities n_H = n(H) + 2n(H_2) ranging between 100 and 1000 cm^(−3)

Excitation and abundance of C_3 in star forming cores: Herschel/HIFI observations of the sight-lines to W31C and W49N

We present spectrally resolved observations of triatomic carbon (C_3) in several ro-vibrational transitions between the vibrational ground state and the low-energy ν_2 bending mode at frequencies between 1654−1897 GHz along the sight-lines to the submillimeter continuum sources W31C and W49N, using Herschel’s HIFI instrument. We detect C_3 in absorption arising from the warm envelope surrounding the hot core, as indicated by the velocity peak position and shape of the line profile. The sensitivity does not allow to detect C_3 absorption due to diffuse foreground clouds. From the column densities of the rotational levels in the vibrational ground state probed by the absorption we derive a rotation temperature (T_(rot)) of ~50−70 K, which is a good measure of the kinetic temperature of the absorbing gas, as radiative transitions within the vibrational ground state are forbidden. It is also in good agreement with the dust temperatures for W31C and W49N. Applying the partition function correction based on the derived T_(rot), we get column densities N(C_3) ~ 7−9 × 10^(14) cm^(−2) and abundance x(C_3) ~ 10^(−8) with respect to H_2. For W31C, using a radiative transfer model including far-infrared pumping by the dust continuum and a temperature gradient within the source along the line of sight we find that a model with x(C_3) = 10^(−8), T_(kin) = 30−50 K, N(C_3) = 1.5 × 10^(15) cm^(−2) fits the observations reasonably well and provides parameters in very good agreement with the simple excitation analysis

Nitrogen hydrides in interstellar gas: Herschel/HIFI observations towards G10.6-0.4 (W31C)

The HIFI instrument on board the Herschel Space Observatory has been used to observe interstellar nitrogen hydrides along the sight-line towards G10.6−0.4 in order to improve our understanding of the interstellar chemistry of nitrogen. We report observations of absorption in NH N = 1 ← 0, J = 2 ← 1 and ortho-NH_2 1_(1,1) ← 0_(0,0). We also observed ortho-NH_3 1_0 ← 0_0, and 2_0 ← 1_0, para-NH_3 2_1 ← 1_1, and searched unsuccessfully for NH^+. All detections show emission and absorption associated directly with the hot-core source itself as well as absorption by foreground material over a wide range of velocities. All spectra show similar, non-saturated, absorption features, which we attribute to diffuse molecular gas. Total column densities over the velocity range 11−54 km s^(−1) are estimated. The similar profiles suggest fairly uniform abundances relative to hydrogen, approximately 6 × 10^(−9), 3 × 10^(−9), and 3 × 10^(−9) for NH, NH_2, and NH_3, respectively. These abundances are discussed with reference to models of gas-phase and surface chemistry

A Herschel/HIFI Legacy Survey of HF and H_2O in the Galaxy: Probing Diffuse Molecular Cloud Chemistry

We combine Herschel observations for a total of 12 sources to construct the most uniform survey of HF and H_2O in our Galactic disk. Both molecules are detected in absorption along all sight lines. The high spectral resolution of the Heterodyne Instrument for the Far-infrared (HIFI) allows us to compare the HF and H_2O distributions in 47 diffuse cloud components sampling the disk. We find that the HF and H2O velocity distributions follow each other almost perfectly and establish that HF and H_2O probe the same gas-phase volume. Our observations corroborate theoretical predictions that HF is a sensitive tracer of H_2 in diffuse clouds, down to molecular fractions of only a few percent. Using HF to trace H_2 in our sample, we find that the N(H_2O)-to-N(HF) ratio shows a narrow distribution with a median value of 1.51. Our results further suggest that H_2O might be used as a tracer of H_2—within a factor of 2.5—in the diffuse interstellar medium (ISM). We show that the measured factor of ~2.5 variation around the median is driven by true local variations in the H_2O abundance relative to H_2 throughout the disk. The latter variability allows us to test our theoretical understanding of the chemistry of oxygen-bearing molecules in the diffuse gas. We show that both gas-phase and grain-surface chemistry are required to reproduce our H_2O observations. This survey thus confirms that grain surface reactions can play a significant role in the chemistry occurring in the diffuse ISM (n_H ≤ 1000 cm^(−3))

Vigilant Keratinocytes Trigger PAMP Signaling in Response to Streptococcal M1 Protein.

The human skin exerts many functions in order to maintain its barrier integrity and protect the host from invading microorganisms. One such pathogen is Streptococcus pyogenes, which can cause a variety of superficial skin wounds that may eventually progress into invasive deep soft tissue infections. Here we show that keratinocytes recognize soluble M1 protein, a streptococcal virulence factor, as a PAMP to release alarming inflammatory responses. We found that this interaction initiates an inflammatory intracellular signaling cascade involving the activation of mitogen-activated kinases, ERK, p38 and JNK, and the subsequent induction and mobilization of the transcription factors NF-κB and AP-1. We also determined the imprint of inflammatory mediators released, such as IL-8, GROα, MIF, EMMPRIN, IL-1α, IL-1Ra, and ST2 in response to streptococcal M1 protein. The expression of IL-8 is dependent on TLR2 activity and subsequent activation of the MAP kinases ERK and p38. Notably this signaling seems distinct for IL-8 release and it is not shared with the other inflammatory mediators. We conclude that keratinocytes participate pro-inflammatory in streptococcal pattern recognition and that expression of the chemoattractant IL-8 by keratinocytes constitutes an important protective mechanism against streptococcal M1 protein

Redox-dependent and redox-independent functions of Caenorhabditis elegans thioredoxin 1

Thioredoxins (TRX) are traditionally considered as enzymes catalyzing redox reactions. However, redox-independent functions of thioredoxins have been described in different organisms, although the underlying molecular mechanisms are yet unknown. We report here the characterization of the first generated endogenous redox-inactive thioredoxin in an animal model, the TRX-1 in the nematode Caenorhabditis elegans. We find that TRX-1 dually regulates the formation of an endurance larval stage (dauer) by interacting with the insulin pathway in a redox-independent manner and the cGMP pathway in a redox-dependent manner. Moreover, the requirement of TRX-1 for the extended longevity of worms with compromised insulin signalling or under calorie restriction relies on TRX-1 redox activity. In contrast, the nuclear translocation of the SKN-1 transcription factor and increased LIPS-6 protein levels in the intestine upon trx-1 deficiency are strictly redox-independent. Finally, we identify a novel function of C. elegans TRX-1 in male food-leaving behaviour that is redox-dependent. Taken together, our results position C. elegans as an ideal model to gain mechanistic insight into the redox-independent functions of metazoan thioredoxins, overcoming the limitations imposed by the embryonic lethal phenotypes of thioredoxin mutants in higher organisms

Detection of hydrogen fluoride absorption in diffuse molecular clouds with Herschel/HIFI: an ubiquitous tracer of molecular gas

We discuss the detection of absorption by interstellar hydrogen fluoride (HF) along the sight line to the submillimeter continuum sources W49N and W51. We have used Herschel's HIFI instrument in dual beam switch mode to observe the 1232.4762 GHz J = 1–0 HF transition in the upper sideband of the band 5a receiver. We detected foreground absorption by HF toward both sources over a wide range of velocities. Optically thin absorption components were detected on both sight lines, allowing us to measure – as opposed to obtain a lower limit on – the column density of HF for the first time. As in previous observations of HF toward the source G10.6–0.4, the derived HF column density is typically comparable to that of water vapor, even though the elemental abundance of oxygen is greater than that of fluorine by four orders of magnitude. We used the rather uncertain N(CH)–N(H_2) relationship derived previously toward diffuse molecular clouds to infer the molecular hydrogen column density in the clouds exhibiting HF absorption. Within the uncertainties, we find that the abundance of HF with respect to H_2 is consistent with the theoretical prediction that HF is the main reservoir of gas-phase fluorine for these clouds. Thus, hydrogen fluoride has the potential to become an excellent tracer of molecular hydrogen, and provides a sensitive probe of clouds of small H_2 column density. Indeed, the observations of hydrogen fluoride reported here reveal the presence of a low column density diffuse molecular cloud along the W51 sight line, at an LSR velocity of ~24 km s^(-1), that had not been identified in molecular absorption line studies prior to the launch of Herschel

Filariosis en mamíferos silvestres del norte de Misiones, Argentina

La Selva Atlántica Interior en Misiones alberga más de 12 especies de carnívoros silvestres (CS), que pueden presentar diferentes parasitosis de importancia zoonótica. La filariosis es causada por nematodes de la Familia Onchocercidae y ha sido reportada en Misiones principalmente en animales domésticos. Este trabajo presenta datos morfológicos y moleculares sobre filariosis halladas en CS de Misiones. Se tomaron muestras de sangre de 100 CS (85 Nasua nasua, 4 Puma concolor, 3 Panthera onca, 2 Leopardus pardalis, 2 Leopardus wiedii, 2 Cerdocyon thous, 1 Eira barbara y 1 Galictis cuja).Asociación Parasitológica Argentin