Imobilizacija Na,K-ATPpaze izolovane iz sinaptičkih plazma-membrana mozga pacova

Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples.Delimično prečišćena Na,K-ATPaza sinaptičkih plazma–membrana (SPM) mozga pacova imobilizovana je adsorpcijom na nitrocelulozne (NC) poliviniliden-fluorid (PVDF) membrane i membrane od staklenih vlakana (SV). Aktivnost enzima delimično prečišćenog solubilizacijom SDS-om povećana je oko 40%. Najveći procenat aktivnosti (14%) enzim zadržava posle 30 minuta adsorpcije SPM na 20ºC, pod vakuumom, sa 25 μg proteina po nitroceluloznom disku. Na,K-ATPaza imobilizovana na nitroceluloznoj membrani stabilna je 72 sata na 20ºC. Adsorpcijom, osetljivost enzima na inhibiciju Hg2+ i Cd2+ se povećava. Rezultati pokazuju da se imobilizovana Na,K-ATPaza SPM može koristiti za detekciju toksičnih metalnih jona u različitim uzorcima

Treatment of Blunt Cerebrovascular Injury - A Systematic Review and Meta-Analysis, Multicenter Retrospective Review, and Protocol for a Feasibility Randomized Controlled Trial

Blunt cerebrovascular injury (BCVI) is an often-overlooked clinical problem that can result in stroke and cause devastating, potentially permanent, neurologic disabilities in young and otherwise healthy trauma patients. Early diagnosis and treatment of BCVI can reduce the risk of stroke and prevent disability, however, treatment also carries a risk of bleeding complications. More research is needed to understand the optimal management strategy to reduce the risk of stroke while minimizing bleeding complications. The aim of this thesis is to review and critically appraise the literature to better understand the efficacy of various treatment strategies in preventing stroke following BCVI, evaluate current practice patterns and patient outcomes at Canadian Level I Trauma Centers, and create a feasibility randomized controlled trial protocol to assess practicability of a future randomized control trial determining the optimal dose of antiplatelet therapy in the treatment of BCVI. Systematic review and meta-analysis of existing literature revealed a slightly lower risk of stroke with the use of antiplatelets compared to anticoagulants (4.5% vs. 5.2%; OR 0.57; 95% CI 0.33 – 0.96, p = 0.04), although there was no difference in stroke rate when evaluating the use of specific agents, acetylsalicylic acid (ASA) vs. heparin (OR 0.43; 95% CI 0.15 – 1.20, p = 0.11). Bleeding complications were significantly higher with the use of anticoagulants and led to more severe bleeding requiring invasive intervention, suggesting better tolerance of antiplatelets in the trauma population. Retrospective review of trauma registries at two Canadian Level I Trauma Centers revealed that patients were more likely to develop stroke after BCVI if they were injured as a result of a motor vehicle collision (MVC), had a lower initial Glasgow Coma Scale (GCS) and higher Injury Severity Scale (ISS), did not meet Denver screening criteria, or had carotid artery injuries. Patients who suffered a stroke were more likely to require intensive care. Treatment interruptions or delays were not associated with increased risk of stroke, and the dose of therapy (81 mg ASA vs. 325 mg ASA) was not independently associated with an increase in stroke rate after adjustment for initial GCS, injury location, and grade of injury (OR 2.244; 95% CI 0.660-7.628). Current research suggests that early detection and treatment of BCVI can significantly reduce the risk of stroke. ASA has shown similar efficacy as heparin for stroke risk reduction but was associated with less bleeding complications. Currently, the optimal dose of ASA is still unknown. Data from retrospective reviews suggests that there is no difference in stroke rates when using low-dose (81 mg) vs. high-dose (325 mg) ASA but no experimental studies exist to evaluate this question. A randomized controlled trial is required to further assess different doses of ASA to determine the optimal dose that reduces the risk of stroke while minimizing bleeding complications

Architectures of an “Otherwise”: Inhabiting Displacement

This concept paper aims to destabilize the limits and delineations of conventional architectural thinking by disclosing its ambiguities and contingencies through the centralization of the inhabitation-displacement nexus and, by extension, the figure of the inhabitant. Building upon our January 2019 workshop at the Max Planck Institute for the Study of Religious and Ethnic Diversity in Germany, entitled: Inside Out – Outside In: Shifting Architectures of Refugee Inhabitation, its keynote interventions by Prof. Anooradha Iyer Siddiqi and Prof. Romola Sanyal, together with the vast array of topics and methodologies brought to the table by our presenters, we primarily argue towards the concept of inhabitation as an epistemic shift of both the site and the subject of architectural (knowledge) production. Shifting our focus particularly to the sites and subjects of displacement (i.e., ‘the refugee’/ ‘the migrant’ and beyond) enabled us to centralize the concept of inhabitation as a modality of the architectural spatial production of an otherwise through the prime subject figure of architectural authorship: the inhabitant

Pharmacological Aspects of Vipera xantina palestinae Venom

In Israel, Vipera xantina palestinae (V.x.p.) is the most common venomous snake, accounting for several hundred cases of envenomation in humans and domestic animals every year, with a mortality rate of 0.5 to 2%. In this review we will briefly address the research developments relevant to our present understanding of the structure and function of V.x.p. venom with emphasis on venom disintegrins. Venom proteomics indicated the presence of four families of pharmacologically active compounds: (i) neurotoxins; (ii) hemorrhagins; (iii) angioneurin growth factors; and (iv) different types of integrin inhibitors. Viperistatin, a α1β1selective KTS disintegrin and VP12, a α2β1 selective C-type lectin were discovered. These snake venom proteins represent promising tools for research and development of novel collagen receptor selective drugs. These discoveries are also relevant for future improvement of antivenom therapy towards V.x.p. envenomation

The OR Black Box as a Novel Tool to Improve Surgical Safety and Education

Errors are prevalent in medicine and frequently lead to increased morbidity and mortality for patients. The complex environment within the operating room, and the multiple people and teams involved in providing patient care, make surgery especially prone to error. Healthcare relies on incident reports, morbidity and mortality (M and M) rounds, and review of patient charts to retrospectively determine factors that contributed to severe errors or near misses.1 Unfortunately, these methods focus primarily on incidents that result in significant patient harm and are subject to recall bias and poor capture of details surrounding key factors or events. Often, many seemingly minor contributions or incidents that do not lead to harm are deemed irrelevant and are not adequately assessed or are omitted altogether. The OR Black Box is a novel system of cameras, monitors, and audio recorders that captures everything that happens in the operating room to allow for future assessment of all errors that occur during a case. Such capture and assessment enable surgeons to review all intraoperative errors and determine what factors lead to errors so they can be avoided in future. The OR Black Box can also be used as an educational tool to facilitate surgical trainee feedback and review of surgical skills. Routine and widespread use of the OR Black Box has the potential to improve surgical safety and training and is a promising new tool for healthcare advancement

Les assistantes maternelles en 2005

International audienceAprès une forte augmentation au cours des années 1990, le nombre d’assistantes maternelles a connu une croissance plus modérée entre 2000 et 2005. Ainsi, les effectifs d’assistantes maternelles agréées en activité et employées directement par les parents sont passés d’environ 70 000 en 1990 à 232 000 en 2000 et atteignent 264 000 en 2005.Cette progression plus modérée de la population des assistantes maternelles en exercice entre 2000 et 2005 s’accompagne d’une stabilité du nombre moyen d’enfants qu’elles sont autorisées à prendre en charge (2,6), ainsi que du nombre moyen d’employeurs par assistante maternelle.Les assistantes maternelles, en moyenne plus âgées que l’ensemble des salariées du secteur privé (45 ans et demi contre 39 ans et demi), vivent plus fréquemment en couple et ont plus souvent des enfants à charge, mais en général déjà scolarisés. Même si leur diplôme le plus haut reste inférieur à celui des salariées du privé, leur niveau d’études continue de s’élever.La moitié des assistantes maternelles déclare travailler plus de 45 heures par semaine et, pour les deux tiers d’entre elles, avec des horaires quotidiens réguliers.Leur salaire mensuel net est en moyenne de 700 euros et de 815 euros pour celles qui travaillent à temps plein

Primary health care of marginalized and refugee populations: An interview with Dr. Allison Henderson

Dr. Allison Henderson is a local family physician with a passion for refugee healthcare and treating London’s most marginalized populations. After completing her medical training and pursuing additional studies at the London School of Hygiene and Tropical Medicine in the UK, she spent over four years working as a physician in rural communities in sub-Saharan Africa. Since returning to London in 2016, she has been working at the London Intercommunity Health Center (LIHC) and Cross Cultural Learner Center (CCLC), where she treats newly arrived Syrian and Yazidi refugees and some of London’s most disadvantaged populations. We had the privilege of meeting Dr. Henderson at the LIHC to discuss her work with refugee populations who have experienced severe trauma and ways that her team is helping them cope and start to heal