Modeling of Chemical Oxygen Demand and Total Suspended Solids Removal Using the Fenton Process

Predictive mathematical models were developed for removing Chemical Oxygen Demand (COD) and Total Suspended Solids (TSS) and optimizing the main operating parameters of the Fenton process, applied to effluents from a fish canning industry. The maximum removals obtained for COD and TSS were 89.2 % and 76.1 %, respectively. The optimum doses for COD removal were: 200 mg/L FeSO4 7H2O and 1,000 mg/L H2O2 at pH 2.5. While for TSS removal the optimum parameters were 1 200 mg/L H2O2, 300 mg/L FeSO4 7H2O, and pH 3. The adjusted R2 values of the COD and TSS removal models were 70.64 % and 98.01 %, respectively, indicating that the models obtained are acceptable in the prediction of both parameters

Different Effect of Proteasome Inhibition on Vesicular Stomatitis Virus and Poliovirus Replication

Proteasome activity is an important part of viral replication. In this study, we examined the effect of proteasome inhibitors on the replication of vesicular stomatitis virus (VSV) and poliovirus. We found that the proteasome inhibitors significantly suppressed VSV protein synthesis, virus accumulation, and protected infected cells from toxic effect of VSV replication. In contrast, poliovirus replication was delayed, but not diminished in the presence of the proteasome inhibitors MG132 and Bortezomib. We also found that inhibition of proteasomes stimulated stress-related processes, such as accumulation of chaperone hsp70, phosphorylation of eIF2α, and overall inhibition of translation. VSV replication was sensitive to this stress with significant decline in replication process. Poliovirus growth was less sensitive with only delay in replication. Inhibition of proteasome activity suppressed cellular and VSV protein synthesis, but did not reduce poliovirus protein synthesis. Protein kinase GCN2 supported the ability of proteasome inhibitors to attenuate general translation and to suppress VSV replication. We propose that different mechanisms of translational initiation by VSV and poliovirus determine their sensitivity to stress induced by the inhibition of proteasomes. To our knowledge, this is the first study that connects the effect of stress induced by proteasome inhibition with the efficiency of viral infection

Complete Genome Sequence of the N2-Fixing Broad Host Range Endophyte Klebsiella pneumoniae 342 and Virulence Predictions Verified in Mice

We report here the sequencing and analysis of the genome of the nitrogen-fixing endophyte, Klebsiella pneumoniae 342. Although K. pneumoniae 342 is a member of the enteric bacteria, it serves as a model for studies of endophytic, plant-bacterial associations due to its efficient colonization of plant tissues (including maize and wheat, two of the most important crops in the world), while maintaining a mutualistic relationship that encompasses supplying organic nitrogen to the host plant. Genomic analysis examined K. pneumoniae 342 for the presence of previously identified genes from other bacteria involved in colonization of, or growth in, plants. From this set, approximately one-third were identified in K. pneumoniae 342, suggesting additional factors most likely contribute to its endophytic lifestyle. Comparative genome analyses were used to provide new insights into this question. Results included the identification of metabolic pathways and other features devoted to processing plant-derived cellulosic and aromatic compounds, and a robust complement of transport genes (15.4%), one of the highest percentages in bacterial genomes sequenced. Although virulence and antibiotic resistance genes were predicted, experiments conducted using mouse models showed pathogenicity to be attenuated in this strain. Comparative genomic analyses with the presumed human pathogen K. pneumoniae MGH78578 revealed that MGH78578 apparently cannot fix nitrogen, and the distribution of genes essential to surface attachment, secretion, transport, and regulation and signaling varied between each genome, which may indicate critical divergences between the strains that influence their preferred host ranges and lifestyles (endophytic plant associations for K. pneumoniae 342 and presumably human pathogenesis for MGH78578). Little genome information is available concerning endophytic bacteria. The K. pneumoniae 342 genome will drive new research into this less-understood, but important category of bacterial-plant host relationships, which could ultimately enhance growth and nutrition of important agricultural crops and development of plant-derived products and biofuels

InContexto: Multisensor Architecture to Obtain People Context from Smartphones

The way users intectact with smartphones is changing after the improvements made in their embedded sensors. Increasingly, these devices are being employed as tools to observe individuals habits. Smartphones provide a great set of embedded sensors, such as accelerometer, digital compass, gyroscope, GPS, microphone, and camera. This paper aims to describe a distributed architecture, called inContexto, to recognize user context information using mobile phones. Moreover, it aims to infer physical actions performed by users such as walking, running, and still. Sensory data is collected by HTC magic application made in Android OS, and it was tested achieving about 97% of accuracy classifying five different actions (still, walking and running).This work was supported in part by Projects CICYT TIN2011-28620-C02-01, CICYT TEC2011-28626-C02-02, CAM CONTEXTS (S2009/TIC-1485), and DPS2008-07029- C02-02.Publicad