Museum visitors’ heterogeneity and experience processing

This research examines the relationships between affective and cognitive antecedents and consequences of satisfaction under a market heterogeneity approach. It includes co-creation of preparatory activities. The sample consisted of 276 museum visitors in London. Two analysis have been conducted: structural equation model and latent class path analysis. The paper contributes to the development of a theoretical framework for further understanding of service experience in which co-creation plays an important role. Two segments were identified: 1) emotional (with lower degree of co-creation, equally distributed by age and nationality); 2) rational (higher degree of co-creation, younger and domestic visitors). Our research shows significant differences between the two segments regarding variables such as satisfaction, loyalty, service experience, emotion, positive disconfirmation and willingness to pay more

Learn-ciam: a model-driven approach for the development of collaborative learning tools

This paper introduces Learn-CIAM, a new model-based methodological approach for the design of flows and for the semi-automatic generation of tools in order to support collaborative learning tasks. The main objective of this work is to help professors by establishing a series of steps for the specification of their learning courses and the obtaining of collaborative tools to support certain learning activities (in particular, for in-group editing, searching and modeling). This paper presents a complete methodological framework, how it is supported conceptually and technologically, and an application example. So to guarantee the validity of the proposal, we also present some validation processes with potential designers and users from different profiles such as Education and Computer Science. The results seem to demonstrate a positive reception and acceptance, concluding that its application would facilitate the design of learning courses and the generation of collaborative learning tools for professionals of both profiles

The influence of digital capabilities on customer orientation of service employees (COSE) and its consequences on customer satisfaction and e-WOM within Family Businesses

Customer relations have become a key matter in services businesses daily activities. Due to the intangibility of services, the employee service performance is what customers perceive as the reflection of the service quality. Due to this fact, the customer orientation of front-line employees, primarily in services industry, plays a crucial role in getting positives/negatives results with regards to customer satisfaction. Customer orientation of service employees (COSE) is a key concept within marketing literature. It has been demonstrated by previous authors the positives effects that has in customer satisfaction and customer retention (Hennig-Thurau. 2004: Morales and Ruiz-Alba, 2018)

Usefulness of gold nanoparticles as labels for the determination of gliadins by immunoaffinity chromatography with light scattering detection

III Encuentro sobre Nanociencia y Nanotecnología de Investigadores y Tecnólogos Andaluce

Upregulation of cardiac NO/NOS system during short-term hypoxia and the subsequent reoxygenation period

Hypoxia/reoxygenation (H/R) reportedly influences nitric oxide (NO) production and NO synthase (NOS) expression in the heart. Nonetheless, a number of works have shown controversial results regarding the changes that the cardiac NO/NOS system undergoes under such situations. Therefore, this study aims to clarify the behaviour of this system in the hypoxic heart by investigating seven different reoxygenation times. Wistar rats were submitted to H/R (hypoxia for 30 min; reoxygenation of 0, 2, 12, 24, 48, 72 h, and 5 days) in a novel approach to address the events provoked by assaults under such circumstances. Endothelial and inducible NOS (eNOS and iNOS) mRNA and protein expression, as well as enzymatic activity and enzyme location were determined. NO levels were indirectly quantified as nitrate/nitrite, and other S-nitroso compounds (NOx), which would act as NO-storage molecules. The results showed a significant increase in eNOS mRNA, protein and activity, as well as in NOx levels immediately after hypoxia, while iNOS protein and activity were induced throughout the reoxygenation period. These findings indicate that, not only short-term hypoxia, but also the subsequent reoxygenation period upregulate cardiac NO/NOS system until at least 5 days after the hypoxic stimulus, implying major involvement of this system in the changes occurring in the heart in response to H/R

International Management of Customer Orientation

This study investigates the role of customer orientation of service employees (COSE) and its influence on customer satisfaction and on electronic word of mouth (e-WOM), with a focus on international management. An empirical study was conducted amongst hotel customers with a final valid sample of 265 respondents. Digital capabilities have been included in the COSE model for the first time. Findings indicate that digital capabilities have a positive influence on customer satisfaction that is mediated by COSE. This study has also compared differences between international and national firms and also considered family businesses and non-family businesses. Results show that international hotels have a higher level of COSE than national hotels, mainly due to the technical skills of the employees, and the presence of a higher level of COSE in family businesses (FBs) rather than in non-family businesses (NFBs). Some contributions to academia and to international management have been discussed

Qué papel juega el hongo Phytophthora cinnamomi en el desarrollo de la regeneración natural y repoblaciones de encina y alcornoque

Se presentan los resultados resumidos de tres trabajos realizados para conocer el papel que juega el hongo Phytophthora cinnamomi en el desarrollo de plántulas de encina (Quercus ilex) y alcornoque (Quercus suber). Se realizaron dos prospecciones para detectar la presencia de P.cinnamomi en focos de seca en dehesas de encina y alcornoque de Extremadura en 1991-1992 y 1999-2000. El porcentaje de focos en los que se detectó la presencia de P. cinnamomi ha permanecido relativamente estable entre las dos prospecciones. También se determinó la tasa de crecimiento relativo para nueve aislados de P. cinnamomi a diferentes temperaturas, observando una elevada variabilidad tanto en la temperatura óptima de crecimiento como en el valor de la propia tasa diaria de crecimiento máxima. También se estudió la emergencia y supervivencia de plántulas de encina y alcornoque procedentes de dos dehesas ecológicamente diferentes, cultivadas en condiciones controladas en suelos de estas dehesas infectados naturalmente con P. cinnamomi y en estos mismos suelos previamente desinfectados en autoclave. Un factor limitante en los procesos de regeneración natural y en las repoblaciones por siembra directa de encinas y alcornoque puede ser su alta susceptibilidad a las infecciones por P. cinnamomi.Por último, se estudió durante dos años consecutivos, la supervivencia de las plantas de encina de una repoblación sobre un suelo naturalmente infectado con P. cinnamomi en la provincia de Badajoz (SO de España). Los resultados obtenidos se discuten teniendo en cuenta el déficit hídrico, los daños por P. cinnamomi, la competencia con otras especies y las posibles medidas sanitarias que amortigüen los daños en las repoblaciones con encinas._______________________________Three different studies were performed with the aim to determine the role of Phytophthora cinnamomi in the early development of seedlings of holm oak (Quercus ilex) and cork oak (Quercus suber). Two surveys, for the presence of Phytophthora cinnamomi in declining holm oak and cork oak stands in Extremadura (SW Spain) were performed in 1991-1992 and 1999-2000. The percentage of decline foci where P. cinnamomi is envolved has remained relatively stable in the lapse of time between the two surveys. Growth rates were determined for 9 P. cinnamomi isolates. Considerable variation in optimum growth temperatures and in daily growth rates occurred among isolates. The emergence and survival of pregerminated holm oak and cork oak acorns from two ecologically different dehesas (Mediterranean open woodlands) were studied in two soils from these stands naturally infected with P. cinnamomi, and in the same soils previously sterilized in the autoclave. The demonstrated high susceptibility of holm and cork oak young seedlings to P. cinnamomi could be a limiting factor in mediterranean opend woodlands (dehesas) not only in natural regenerationprocesses but also when reforestation by direct sowing is implemented. Finally, the viability of 1 year old holm oak seedlings in a soil naturally infected with P. cinnamomi was studied during two consecutive years in a plot located in SW Spain. Results obtained are discussed in the framework of seasonal water deficit, P. cinnamomi damage, weed competition and sanitation thechniques to be used in declined holm oak stands in Spain

Bioinformaticsas educational resource:Genetic engineering project

La utilización de medios informáticos para procesar los contenidos curriculares de las diferentes materias, así como para aprender su propio manejo, ha crecido exponencialmente en los últimos años. El uso de las Tecnologías de la Información y la Comunicación (TIC) pueden contribuir al acceso universal a la educación mediante microproyectos de bajo coste. Sin embargo, para ello hay que diseñar y desarrollar proyectos educativos coherentes con las necesidades educativas. Con este proyecto, programas bioinformáticos básicos necesarios para el desarrollo de un proyecto de ingeniería genética se transformarán en recursos educativos con los que el alumnado no solo adquirirá nociones básicas sobre el uso y manejo de estos programas -que les serán requeridos en su vida profesional como científicos-, sino que les servirá para afianzar y contextualizar conceptos relacionados con el propio proceso. Partiendo del objetivo de la mutación de un gen concreto, el alumnado será capaz de desarrollar una estrategia que le reporte una gran cantidad de información sobre éste usando únicamente medios informáticos.The use computational toolsto process curricular contents of the different subjects, as well as to learn their own management, has grown exponentially in recent years. The use of Information and Communication Technologies (ICT) can contribute to universal access to education, equality in education, the exercise of quality teaching andto thelearning and professional development of teachers, as well as to a moreefficient management and administration of the education system. However, it is necessary to design and develop educational projects that are consistent with educational needs. With thisproject, basedon the use of basic bioinformatics programs within the framework of a genetic engineering project, students will not only acquire basic notions about the use and management of these programs, which will be required in their professional life as scientists, but also it will serve to strengthen and contextualize concepts related to the process itself. Based on the objective of mutation of a particular gene, students will be able to develop a strategy that will report a large amount of information about it using only computational tools