3 research outputs found
Myeloid cell distribution and activity in multiple sclerosis
Multiple sclerosis (MS) is a demyelinating
disease in which an exacerbated immune response
provokes oligodendrocyte loss and demyelination, the
hallmarks of this neurological disease. The destruction
of myelin due to the uncontrolled activity of the
invading immune cells leads to the formation of MS
plaques. Among the different leukocytes that participate
in the immune response associated with MS, the role of
myeloid cells has been analyzed extensively (i.e.
macrophages, dendritic cells -DCs- and neutrophils).
Hence, in this review we will summarize what is known
about the distribution, expression and markers available
to study myeloid cells, and their histopathology, not only
in a standard animal model of MS (autoimmune
experimental encephalomyelitis -EAE) but also in MS
tissue. In this review, we will not only refer to mature
myeloid cells but also to the undifferentiated and almost
unexplored myeloid-derived suppressor cells (MDSCs).
The active role of MDSCs in the prompt resolution of an
immune episode is gaining importance, yet is still the
subject of some debate. Finally, the similarities and
differences between MS and EAE are discussed,
particularly in terms of myeloid cell phenotype, activity
and the markers used
Myeloid-derived suppressor cells limit the inflammation by promoting T lymphocyte apoptosis in the spinal cord of a murine model of multiple sclerosis.
Multiple Sclerosis (MS) is a demyelinating/inflammatory disease of the central nervous system. Relapsing-remitting MS is characterized by a relapsing phase with clinical symptoms and the production of inflammatory cell infiltrates, and a period of remission during which patients recover partially. Myeloid-derived suppressor cells (MDSCs) are immature cells capable of suppressing the inflammatory response through Arginase-I (Arg-I) activity, among other mechanisms. Here, we have identified Arg-I+-MDSCs in the spinal cord during experimental autoimmune encephalomyelitis (EAE), cells that were largely restricted to the demyelinating plaque and that always exhibited the characteristic MDSC surface markers Arg-I/CD11b/Gr-1/M-CSF1R. The presence and density of Arg-I+-cells, and the proportion of apoptotic but not proliferative T cells, were correlated with the EAE time course: peaked in parallel with the clinical score, decreased significantly during the remitting phase and completely disappeared during the chronic phase. Spinal cord-isolated MDSCs of EAE animals augmented the cell death when co-cultured with stimulated control splenic CD3 T cells. These data point to an important role for MDSCs in limiting inflammatory damage in MS, favoring the relative recovery in the remitting phase of the disease. Thus, the MDSC population should be considered as a potential therapeutic target to accelerate the recovery of MS patients.This work was supported by the Ministerio de Ciencia e Innovación-MICINN (SAF2009-07842); Fondo de Investigaciones Sanitarias-FIS (partially funded by F.E.D.E.R.- European Union—“Una manera de hacer Europa”) (RD07-0060-2007); and Gobierno de Castilla-La Mancha (PAI08-0242-3822; ICS06024-00, G-2008-C8; PI2009-26). VMV and MCO are FISCAM fellows (MOV-2009_JI-01 and MOV-2007_JI-20, respectively). DC, VV and FdC are hired by SESCAM.Peer reviewe