Enzyme-magnetic nanoparticle reactor for the advanced oxidation of micropollutants in wastewaters

Oxidative biocatalysts by oxidoreductases arises as a promising alternative from the development of advanced oxidation processes for transformation of emerging contaminants to transformation of compounds into value added bio-based products. For technical and economic reasons, in most enzyme catalysed processes it is necessary to reuse the biocatalyst. In this context, enzyme immobilization can be defined as a technique that allows reuse or continued use of the biocatalyst. Envisaging the application of the different biocatalysts in wastewater treatment plants or other biotechnological applications it is important to design an enzymatic reactor in which the recovery and reuse of the enzyme is fulfilled

La discapacidad: De reto a oportunidad en la prevención de riesgos laborales

En la actualidad se estima que solo en la Unión Europea hay más de 100 millones de personas que tienen algún tipo de discapacidad. Sin embargo, 3 de cada 4 personas con discapacidad en España no tienen empleo y en la mayoría de los casos es debido a entre otros factores a no disponer de un sistema de gestión de la prevención de riesgos laborales inclusivo. Entre las claves de la gestión inclusiva se encuentra: la adaptación de puestos de trabajo, la creación de una cultura organizacional inclusiva, entre otras. Un claro ejemplo de empresa inclusiva es la Organización Nacional de Ciegos Españoles (ONCE) dónde todos los trabajadores son iguales y dispone de un sistema de gestión certificado con la norma ISO 45001:2018

Formulation of Laccase Nanobiocatalysts Based on Ionic and Covalent Interactions for the Enhanced Oxidation of Phenolic Compounds

Oxidative biocatalysis by laccase arises as a promising alternative in the development of advanced oxidation processes for the removal of xenobiotics. The aim of this work is to develop various types of nanobiocatalysts based on laccase immobilized on different superparamagnetic and non-magnetic nanoparticles to improve the stability of the biocatalysts. Several techniques of enzyme immobilization were evaluated based on ionic exchange and covalent bonding. The highest yields of laccase immobilization were achieved for the covalent laccase nanoconjugates of silica-coated magnetic nanoparticles (2.66 U mg−1 NPs), formed by the covalent attachment of the enzyme between the aldehyde groups of the glutaraldehyde-functionalized nanoparticle and the amino groups of the enzyme. Moreover, its application in the biotransformation of phenol as a model recalcitrant compound was tested at different pH and successfully achieved at pH 6 for 24 h. A sequential batch operation was carried out, with complete recovery of the nanobiocatalyst and minimal deactivation of the enzyme after four cycles of phenol oxidation. The major drawback associated with the use of the nanoparticles relies on the energy consumption required for their production and the use of chemicals, that account for a major contribution in the normalized index of 5.28 × 10−3. The reduction of cyclohexane (used in the synthesis of silica-coated magnetic nanoparticles) led to a significant lower index (3.62 × 10−3); however, the immobilization was negatively affected, which discouraged this alternativeThis work was financially supported by the Spanish Ministry of Economy and Competitiveness (CTQ2013-44762-R and CTQ2016-79461-R, program co-funded by FEDER). The authors belong to the Galician Competitive Research Group GRC 2013-032, program co-funded by FEDER. Yolanda Moldes-Diz thanks the Spanish Ministry of Economy and Competitiveness for her predoctoral fellowshipS

Formulation of Laccase Nanobiocatalysts Based on Ionic and Covalent Interactions for the Enhanced Oxidation of Phenolic Compounds

Oxidative biocatalysis by laccase arises as a promising alternative in the development of advanced oxidation processes for the removal of xenobiotics. The aim of this work is to develop various types of nanobiocatalysts based on laccase immobilized on different superparamagnetic and non-magnetic nanoparticles to improve the stability of the biocatalysts. Several techniques of enzyme immobilization were evaluated based on ionic exchange and covalent bonding. The highest yields of laccase immobilization were achieved for the covalent laccase nanoconjugates of silica-coated magnetic nanoparticles (2.66 U mg−1 NPs), formed by the covalent attachment of the enzyme between the aldehyde groups of the glutaraldehyde-functionalized nanoparticle and the amino groups of the enzyme. Moreover, its application in the biotransformation of phenol as a model recalcitrant compound was tested at different pH and successfully achieved at pH 6 for 24 h. A sequential batch operation was carried out, with complete recovery of the nanobiocatalyst and minimal deactivation of the enzyme after four cycles of phenol oxidation. The major drawback associated with the use of the nanoparticles relies on the energy consumption required for their production and the use of chemicals, that account for a major contribution in the normalized index of 5.28 × 10−3. The reduction of cyclohexane (used in the synthesis of silica-coated magnetic nanoparticles) led to a significant lower index (3.62 × 10−3); however, the immobilization was negatively affected, which discouraged this alternative

VI Encontro da Mocidade Investigadora: Libro de resumos

Libro de resumos correspondentes ás presentacións durante o VI Encontro da Mocidade Investigadora - CienciasUniversidade de Santiago de Compostela. Centro Internacional de Estudos de Doutoramento e Estudos Avanzado