THE PROJECT METHODS FOR CALCULATION THE PILES CAPACITY ON THE PRACTICAL CASE

Diplomsko delo je sestavljeno iz treh sklopov in zajema teoretični in praktični del, ki se nanaša na globoko temeljenje obroča hladilnega stolpa bloka 6 v termoelektrarni Šoštanj. V prvem delu so predstavljene osnovne značilnosti pilota, ki se nanašajo na vrste pilotov, način in tehnologijo vgradnje. V drugem delu je predstavljena faza temeljenja z navedbo opravljenih laboratorijskih preiskav, opisom terenskih preiskav, kot sta: SPT in PMT in opisom računskih metod za analizo tlačne nosilnosti pilota na podlagi rezultatov iz presiometra z upoštevanjem standarda SIST EN 1997-1. V tretjem delu je predstavljen projekt z geološkimi značilnostmi, rezultati terenskih preiskav, analizo nosilnosti in uporabnosti ter primerjavo uporabljenih računskih metod z statičnim obremenilnim testom na terenu.The thesis is divided into three sections with theoretical and practical part which refers to deep foundations ring cooling tower 6 of thermal power plant Šoštanj. The first section presents basic characteristics of the piles referring to the types of pile, method and built-in technology. The second section presents the foundation stage with completed laboratory tests, field investigation description such as: SPT and PMT, and with description of calculation methods used for the compressive resistance analysis of pile based on pressuremeter results according to SIST EN 1997-1 standard. The third section refers to geological characteristics of the location, the field investigation results, the ultimate compressive resistance analysis, the serviceability limit state analysis under vertical load and the comparison of used calculation methods with static load test on the field

Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells

Background: The ability of adipose tissue-derived multipotent mesenchymal stromal cells/mesenchymal stem cells (ASCs) to differentiate in neural lineages promises progress in the field of regenerative medicine, especially for replacing neuronal tissue damaged by different neurological disorders. Reprogramming of ASCs can be induced by the growth medium with neurogenic inductors and specific growth factors. We investigated the neural differentiation potential of canine ASCs using several growth media (KEM, NIMa, NIMb, NIMc) containing various combinations of neurogenic inductors: B27 supplement, valproic acid, forskolin, N2-supplement, and retinoic acid. Cells were first preconditioned in the pre-differentiation neural induction medium (mitogenically stimulatedSTIM1), followed by the induction of neuronal differentiation. Results: After 3, 6, and 9 days of neural induction, elongated neural-like cells with bipolar elongations were observed, and some oval cells with light nuclei appeared. The expression of neuronal markers tubulin beta III (TUBB3), neurofilament H (NF-H), microtubule-associated protein-2 (MAP2), and glial fibrillary acidic protein (GFAP) was observed using immunocytochemistry, which confirmed the differentiation into neurons and glial cells. Flow cytometry analysis showed high GFAP expression (between 70 and 90% of all cells) after cells had been growing three days in the neural induction medium a (NIMa). Around 25% of all cells also expressed adult neuronal markers NF-H and MAP2. After nine days of ASCs differentiation, the expression of all neural markers was reduced. There were no differences between the neural differentiation of ASCs isolated from female or male dogs. Conclusions: The differentiation repertoire of canine ASCs extends beyond mesodermal lineages. Using a defined neural induction medium, the canine ASCs differentiated into neural lineages and expressed markers of neuronal and glial cells, and also displayed the typical neuronal morphology. Differentiated ASCs can thus be a source of neural cellular lineages for the regenerative therapy of nerve damage and could be useful in the future for therapy or the modelling of neurodegenerative diseases