Utilization of Some Fruits and Vegetables By-Products to Produce High Dietary Fiber Jam

The present study aimed to investigate the chemical composition, antioxidant activity, total phenolic compounds and ?-carotene of carrot peels, apple pomace, banana peels and mandarin peels and their quality in preparing jam. Mandarin and banana peels characterized by its higher crude fiber (12.16 and 5.25%) and vitamin C (68 and 16.6 mg/100g) compared to carrot peels (3.91%) and apple pomace (3.65%). Banana peels contained higher amount of magnesium, potassium, calcium and iron compared to other peels samples. Therefore, jam of banana peels characterized by its higher content in magnesium (758 mg/100g), potassium (779 mg/100g), calcium (191 mg/100g) and iron (59.15 mg/100g). Jam of apple pomace characterized by its higher phosphorus contents (220 mg/100g) followed by jam of banana peels (138 mg/100g), mandarin peels (128 mg/100g) and carrot peels (53 mg/100g). Jam of carrot peels characterized by its higher phenolics content as gallic acid equivalent (87.4 mg/100g) followed by jams of apple pomace (82.5 mg/100g), banana peels (42.7 mg/100g) and mandarin peels (34.6 mg/100g). The same trend was observed in total flavonoids as catechen equivalent (mg CAT/100g) in jams of carrot peels, apple pomace, banana peels and mandarin peels, where they were 35.9, 30.1, 23.5 and 21.7, respectively. Furthermore, jam of carrot peels had higher antioxidant activity, where its DPPH radical, had lower DPPH based IC50 (1.8 ?g/ml) while jam of apple pomace, banana peels and mandarin peels had higher DPPH based IC50 reached to 2.04, 2.21 and 3.34 µg/ml, respectively. The same trend was observed for the ?-carotene radical in tested jam samples. Hunter color parameter showed that jam of mandarin peels had highest lightness (L* = 39.8), followed by jam of carrot peels (29.46), apple pomace (18.27) and banana peels (15.19). Therefore, jam of banana peels was darker than other tested peels samples. Sensory evaluation showed that jam of apple pomace characterized by its higher taste and odor, followed by jam of mandarin peels, banana peels and carrot peels. Color of tested jam of carrot, banana or mandarin peels was darker than apple pomace jam. Also, jam of apple pomace gave higher scores in appearance and overall acceptability. Keywords: Jam – Peels – antioxidant activity – Total phenolics– Total Flavonoid

FOURIER TRANSFORM INFRARED SPECTROSCOPY TECHNIQUE FOR DETECTION OF HONEY AUTHENTICATION

Physico-chemical properties of honey and honey samples adulterated with glucose or sucrose were determined. Total soluble solids (TSS), pH and electrical conductivity of honey and its adulterated samples ranged between (84.10-84.50%), (3.80-4.63) and (11.73 – 232.32µS), respectively. Sensory properties of honey and its adulterated samples showed that, no differences in the sensorial properties were found in authentic honey and honey adulterated with 25% sucrose or 25% glucose. Increasing adulteration ratio to 50% glucose decreased taste, flavor, color and general appearance, while adulterated honey with 50% sucrose caused significant decrease in taste and general appearance. The possibility of using HPLC to detect adulteration of honey through determining their sugars content was evaluated. The obtained results indicated that, authentic honey was characterized with its higher fructose/glucose ratio (1.21) compared to adulterated honey samples (ranged between 0.35 - 0.94). FT-IR spectroscopic technique was used to evaluate honey quality. Reliability FT-IR for quantitative and qualitative analysis of sucrose, glucose and fructose were evaluated.  The main FT-IR spectral bands of sucrose, glucose and fructose were identified at different concentration levels. The relationship between sugars concentration (sucrose, glucose and fructose) and its spectral bands absorbance (peak height) were evaluated to prepare sugars standard curves and their linear equations. Selected main peaks of sucrose, glucose and fructose provide the best calibration model with correlation coefficient (r2) higher than (0.9). Honey samples adulterated with glucose were characterized with specific spectral peaks, in which the absorbance was increased by increasing the ratio of adulteration with glucose at 1087, 1105, 1189 and 984 cm-1, while the adulteration with sucrose lead to increase in the absorbance of spectral bands of sucrose as 1054, 1149 and 984 cm-1 especially in honey adulterated with 50 % sucrose

Excess Spin and the Dynamics of Antiferromagnetic Ferritin

Temperature-dependent magnetization measurements on a series of synthetic ferritin proteins containing from 100 to 3000 Fe(III) ions are used to determine the uncompensated moment of these antiferromagnetic particles. The results are compared with recent theories of macroscopic quantum coherence which explicitly include the effect of this excess moment. The scaling of the excess moment with protein size is consistent with a simple model of finite size effects and sublattice noncompensation.Comment: 4 pages, 3 Postsript figures, 1 table. Submitted to PR

Statistical inference for the Nadarajah-Haghighi distribution based on ranked set sampling with applications

In this article, the maximum likelihood and Bayes inference methods are discussed for determining the two unknown parameters and specific lifetime parameters of the Nadarajah-Haghighi distribution, such as the survival and hazard rate functions, with the inclusion of ranked set sampling and simple random sampling. The estimated confidence intervals for the two parameters and any function of them are developed based on the Fisher-information matrix. Metropolis-Hastings algorithm and Lindley-approximation are used for generating the Bayes estimates and related highest posterior density credible ranges for the unknown parameters and reliability parameters under the presumption of conjugate gamma priors. A Monte-Carlo simulation study and a real-life data set have been used to assess the efficacy of the proposed methods

Estimation in Step-Stress Accelerated Life Tests for Weibull Distribution with Progressive First-Failure Censoring

Abstract: Based on progressive first-failure censoring, step-stress partially accelerated life tests are considered when the lifetime of a product follows Weibull distribution. The maximum likelihood estimates (MLEs) are obtained for the distribution parameters and the acceleration factor. In addition, asymptotic variance and covariance matrix of the estimators are given. Furthermore, confidence intervals of the estimators are presented. The optimal stress change time for the step-stress partially accelerated life test is determined by minimizing the asymptotic variance of MLEs of the model parameters and the acceleration factor. Simulation results are carried out to study the precision of the MLEs for the parameters involved

Validated stability indicating liquid chromatographic determination of ebastine in pharmaceuticals after pre column derivatization: Application to tablets and content uniformity testing

An accurate, simple, sensitive and selective reversed phase liquid chromatographic method has been developed for the determination of ebastine in its pharmaceutical preparations. The proposed method depends on the complexation ability of the studied drug with Zn2+ ions. Reversed phase chromatography was conducted using an ODS C18 (150 × 4.6 mm id) stainless steel column at ambient temperature with UV-detection at 260 nm. A mobile phase containing 0.025%w/v Zn2+ in a mixture of (acetonitril/methanol; 1/4) and Britton Robinson buffer (65:35, v/v) adjusted to pH 4.2, has been used for the determination of ebastine at a flow rate of 1 ml/min. The calibration curve was rectilinear over the concentration range of 0.3 - 6.0 μg/ml with a detection limit (LOD) of 0.13 μg/ml, and quantification limit (LOQ) of 0.26 μg/ml. The proposed method was successfully applied for the analysis of ebastine in its dosage forms, the obtained results were favorably compared with those obtained by a comparison method. Furthermore, content uniformity testing of the studied pharmaceutical formulations was also conducted. The composition of the complex as well as its stability constant was also investigated. Moreover, the proposed method was found to be a stability indicating one and was utilized to investigate the kinetics of alkaline and ultraviolet induced degradation of the drug. The first-order rate constant and half life of the degradation products were calculated

Validated stability-indicating spectrofluorimetric methods for the determination of ebastine in pharmaceutical preparations

Two sensitive, selective, economic, and validated spectrofluorimetric methods were developed for the determination of ebastine (EBS) in pharmaceutical preparations depending on reaction with its tertiary amino group. Method I involves condensation of the drug with mixed anhydrides (citric and acetic anhydrides) producing a product with intense fluorescence, which was measured at 496 nm after excitation at 388 nm

Green Synthesis of Zinc Oxide Nanoparticles from Althaea officinalis Flower Extract Coated with Chitosan for Potential Healing Effects on Diabetic Wounds by Inhibiting TNF-α and IL-6/IL-1β Signaling Pathways

Sammar Fathy Elhabal,1 Nashwa Abdelaal,2 Saeed Abdul Kareem Saeed Al-Zuhairy,3 Mohamed Fathi Mohamed Elrefai,4,5 Ahmed Mohsen Elsaid Hamdan,6 Mohamed Mansour Khalifa,7 Sandra Hababeh,8 Mohammad Ahmad Khasawneh,9 Gehad M Khamis,10 Jakline Nelson,11 Passant M Mohie,10 Rania A Gad,12 Amira Rizk,13 Soad L Kabil,14 Mohamed Kandeel El-Ashery,15,16 Bhaskara R Jasti,17 Nahla A Elzohairy,18,19 Tayseer Elnawawy,20 Fatma E Hassan,21,22 Mohamed A El- Nabarawi23 1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Mokattam, Cairo, Egypt; 2Department of Integrative Physiology, Baylor College of Medicine, Houston, TX, USA; 3Department of Pharmacy, Kut University College, Kut, Wasit, Iraq; 4Department of Anatomy, Histology, Physiology and Biochemistry, Faculty of Medicine, The Hashemite University, Zarqa, Jordan; 5Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo, Egypt; 6Department of Pharmacy Practice, Faculty of Pharmacy, University of Tabuk, Tabuk, Saudi Arabia; 7Department of Human Physiology, Faculty of Medicine, Cairo University, Cairo, Egypt; 8Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; 9Department of Chemistry, College of Science U.A.E. University, Al-Ain, United Arab Emirates; 10Department of Clinical Pharmacology, Faculty of Medicine, Alexandria University, Alexandria, Egypt; 11Department of Microbiology and Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt; 12Department of Pharmacology and Toxicology, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt; 13Food Science and Technology Department, Faculty of Agricultural, Tanta University, Tanta, Egypt; 14Department of Clinical Pharmacology, Faculty of Medicine, Zagazig University, Zagazig, Egypt; 15Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 16Medicinal Chemistry Department, Faculty of Pharmacy, King Salman International University, Ras-Sedr, South Sinai, Egypt; 17Department of Pharmaceutics and Medicinal Chemistry, Thomas J. Long School of Pharmacy, University of the Pacific, Stockton, CA, USA; 18Air Force Specialized Hospital, Cairo, Egypt; 19Department of Microbiology and Immunology, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Mokattam, Cairo, Egypt; 20Department of Pharmaceutics, Egyptian Drug Authority, Cairo, Egypt; 21Medical Physiology Department, Faculty of Medicine, Cairo University, Giza, Egypt; 22General Medicine Practice Program, Department of Physiology, Batterjee Medical College, Jeddah, Saudi Arabia; 23Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, EgyptCorrespondence: Sammar Fathy Elhabal, Email [email protected]; [email protected]; Mohamed A El- Nabarawi, Email [email protected]: Diabetes Mellitus is a multisystem chronic pandemic, wound inflammation, and healing are still major issues for diabetic patients who may suffer from ulcers, gangrene, and other wounds from uncontrolled chronic hyperglycemia. Marshmallows or Althaea officinalis (A.O.) contain bioactive compounds such as flavonoids and phenolics that support wound healing via antioxidant, anti-inflammatory, and antibacterial properties. Our study aimed to develop a combination of eco-friendly formulations of green synthesis of ZnO-NPs by Althaea officinalis extract and further incorporate them into 2% chitosan (CS) gel.Method and Results: First, develop eco-friendly green Zinc Oxide Nanoparticles (ZnO-NPs) and incorporate them into a 2% chitosan (CS) gel. In-vitro study performed by UV-visible spectrum analysis showed a sharp peak at 390 nm, and Energy-dispersive X-ray (EDX) spectrometry showed a peak of zinc and oxygen. Besides, Fourier transforms infrared (FTIR) was used to qualitatively validate biosynthesized ZnO-NPs, and transmission electron microscope (TEM) showed spherical nanoparticles with mean sizes of 76 nm and Zeta potential +30mV. The antibacterial potential of A.O.-ZnO-NPs-Cs was examined by the diffusion agar method against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Based on the zone of inhibition and minimal inhibitory indices (MIC). In addition, an in-silico study investigated the binding affinity of A.O. major components to the expected biological targets that may aid wound healing. Althaea Officinalis, A.O-ZnO-NPs group showed reduced downregulation of IL-6, IL-1β, and TNF-α and increased IL-10 levels compared to the control group signaling pathway expression levels confirming the improved anti-inflammatory effect of the self-assembly method. In-vivo study and histopathological analysis revealed the superiority of the nanoparticles in reducing signs of inflammation and wound incision in rat models.Conclusion: These biocompatible green zinc oxide nanoparticles, by using Althaea Officinalis chitosan gel ensure an excellent new therapeutic approach for quickening diabetic wound healing. Keywords: wound healing, antimicrobial, antioxidant, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, chitosan, wound concentration, wound incisio