Sequencing, Analysis, and Annotation of Expressed Sequence Tags for Camelus dromedarius

Despite its economical, cultural, and biological importance, there has not been a large scale sequencing project to date for Camelus dromedarius. With the goal of sequencing complete DNA of the organism, we first established and sequenced camel EST libraries, generating 70,272 reads. Following trimming, chimera check, repeat masking, cluster and assembly, we obtained 23,602 putative gene sequences, out of which over 4,500 potentially novel or fast evolving gene sequences do not carry any homology to other available genomes. Functional annotation of sequences with similarities in nucleotide and protein databases has been obtained using Gene Ontology classification. Comparison to available full length cDNA sequences and Open Reading Frame (ORF) analysis of camel sequences that exhibit homology to known genes show more than 80% of the contigs with an ORF>300 bp and ~40% hits extending to the start codons of full length cDNAs suggesting successful characterization of camel genes. Similarity analyses are done separately for different organisms including human, mouse, bovine, and rat. Accompanying web portal, CAGBASE (http://camel.kacst.edu.sa/), hosts a relational database containing annotated EST sequences and analysis tools with possibility to add sequences from public domain. We anticipate our results to provide a home base for genomic studies of camel and other comparative studies enabling a starting point for whole genome sequencing of the organism

Sequencing, Analysis, and Annotation of Expressed Sequence Tags for \u3ci\u3eCamelus dromedarius\u3c/i\u3e

Despite its economical, cultural, and biological importance, there has not been a large scale sequencing project to date for Camelus dromedarius. With the goal of sequencing complete DNA of the organism, we first established and sequenced camel EST libraries, generating 70,272 reads. Following trimming, chimera check, repeat masking, cluster and assembly, we obtained 23,602 putative gene sequences, out of which over 4,500 potentially novel or fast evolving gene sequences do not carry any homology to other available genomes. Functional annotation of sequences with similarities in nucleotide and protein databases has been obtained using Gene Ontology classification. Comparison to available full length cDNA sequences and Open Reading Frame (ORF) analysis of camel sequences that exhibit homology to known genes show more than 80% of the contigs with an ORF\u3e300 bp and ~40% hits extending to the start codons of full length cDNAs suggesting successful characterization of camel genes. Similarity analyses are done separately for different organisms including human, mouse, bovine, and rat. Accompanying web portal, CAGBASE (http://camel.kacst.edu.sa/), hosts a relational database containing annotated EST sequences and analysis tools with possibility to add sequences from public domain. We anticipate our results to provide a home base for genomic studies of camel and other comparative studies enabling a starting point for whole genome sequencing of the organism

The Influence of Cc-normality Subgroups on the Structure of Finite Groups

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Morita context and Generalized (α, β)−Derivations

Let $R$ and $S$ be rings of a semi-projective Morita context, and $alpha, eta$ be automorphisms of $R$. An additive mapping $F$: $Ro R$ is called a generalized $(alpha,eta)$-derivation on $R$ if there exists an $(alpha,eta)$-derivation $d$: $Ro R$ such that$F(xy)=F(x)alpha(y)+eta(x)d(y)$ holds for all $x,y in R$. For any $x,y in R$, set $[x, y]_{alpha, eta} = x alpha(y) - eta(y) x$ and $(x circ y)_{alpha, eta} = x alpha(y) + eta(y) x$. In the present paper, we shall show that if the ring $S$ is reduced then it is a commutative, in a compatible way with the ring $R$ . Also, we obtain some results on bialgebras via Cauchy modules

Characterization of Soft S-Open Sets in Bi-Soft Topological Structure Concerning Crisp Points

In this article, a soft s-open set in soft bitopological structures is introduced. With the help of this newly defined soft s-open set, soft separation axioms are regenerated in soft bitopological structures with respect to crisp points. Soft continuity at some certain points, soft bases, soft subbase, soft homeomorphism, soft first-countable and soft second-countable, soft connected, soft disconnected and soft locally connected spaces are defined with respect to crisp points under s-open sets in soft bitopological spaces. The product of two soft  axioms with respect crisp points with almost all possibilities in soft bitopological spaces relative to semiopen sets are introduced. In addition to this, soft (countability, base, subbase, finite intersection property, continuity) are addressed with respect to semiopen sets in soft bitopological spaces. Product of soft first and second coordinate spaces are addressed with respect to semiopen sets in soft bitopological spaces. The characterization of soft separation axioms with soft connectedness is addressed with respect to semiopen sets in soft bitopological spaces. In addition to this, the product of two soft topological spaces is (  space if each coordinate space is soft  space, product of two sot topological spaces is (S regular and C regular) space if each coordinate space is (S regular and C regular), the product of two soft topological spaces is connected if each coordinate space is soft connected and the product of two soft topological spaces is (first-countable, second-countable) if each coordinate space is (first countable, second-countable)

Some Results in Neutrosophic Soft Topology Concerning Neutrosophic Soft ∗b Open Sets

In this article, new generalised neutrosophic soft open known as neutrosophic soft ∗b open set is introduced in neutrosophic soft topological spaces. Neutrosophic soft ∗bopen set is generated with the help of neutrosophic soft semiopen and neutrosophic soft preopen sets. Then, with the application of this new definition, some soft neutrosophical separation axioms, countability theorems, and countable space can be Hausdorff space under the subjection of neutrosophic soft sequence which is convergent, the cardinality of neutrosophic soft countable space, engagement of neutrosophic soft countable and uncountable spaces, neutrosophic soft topological features of the various spaces, soft neutrosophical continuity, the product of different soft neutrosophical spaces, and neutrosophic soft countably compact that has the characteristics of Bolzano Weierstrass Property (BVP) are studied. In addition to this, BVP shifting from one space to another through neutrosophic soft continuous functions, neutrosophic soft sequence convergence, and its marriage with neutrosophic soft compact space, sequentially compactness are addressed

Sequencing, Analysis, and Annotation of Expressed Sequence Tags for \u3ci\u3eCamelus dromedarius\u3c/i\u3e

Despite its economical, cultural, and biological importance, there has not been a large scale sequencing project to date for Camelus dromedarius. With the goal of sequencing complete DNA of the organism, we first established and sequenced camel EST libraries, generating 70,272 reads. Following trimming, chimera check, repeat masking, cluster and assembly, we obtained 23,602 putative gene sequences, out of which over 4,500 potentially novel or fast evolving gene sequences do not carry any homology to other available genomes. Functional annotation of sequences with similarities in nucleotide and protein databases has been obtained using Gene Ontology classification. Comparison to available full length cDNA sequences and Open Reading Frame (ORF) analysis of camel sequences that exhibit homology to known genes show more than 80% of the contigs with an ORF\u3e300 bp and ~40% hits extending to the start codons of full length cDNAs suggesting successful characterization of camel genes. Similarity analyses are done separately for different organisms including human, mouse, bovine, and rat. Accompanying web portal, CAGBASE (http://camel.kacst.edu.sa/), hosts a relational database containing annotated EST sequences and analysis tools with possibility to add sequences from public domain. We anticipate our results to provide a home base for genomic studies of camel and other comparative studies enabling a starting point for whole genome sequencing of the organism

Sequencing, analysis, and annotation of expressed sequence tags for Camelus dromedarius.

Despite its economical, cultural, and biological importance, there has not been a large scale sequencing project to date for Camelus dromedarius. With the goal of sequencing complete DNA of the organism, we first established and sequenced camel EST libraries, generating 70,272 reads. Following trimming, chimera check, repeat masking, cluster and assembly, we obtained 23,602 putative gene sequences, out of which over 4,500 potentially novel or fast evolving gene sequences do not carry any homology to other available genomes. Functional annotation of sequences with similarities in nucleotide and protein databases has been obtained using Gene Ontology classification. Comparison to available full length cDNA sequences and Open Reading Frame (ORF) analysis of camel sequences that exhibit homology to known genes show more than 80% of the contigs with an ORF>300 bp and approximately 40% hits extending to the start codons of full length cDNAs suggesting successful characterization of camel genes. Similarity analyses are done separately for different organisms including human, mouse, bovine, and rat. Accompanying web portal, CAGBASE (http://camel.kacst.edu.sa/), hosts a relational database containing annotated EST sequences and analysis tools with possibility to add sequences from public domain. We anticipate our results to provide a home base for genomic studies of camel and other comparative studies enabling a starting point for whole genome sequencing of the organism

Numbers of unique GeneIDs, GO Terms that are mapped by the ESTs with hits for the nine species analyzed.

<p>Numbers of GeneIDs, GO Terms, and GeneIDs that have a GO annotation are shown for the nine species analyzed, where applicable. For each camel sequence group (contig, singleton, and combination of the two), number of unique GeneIDs that are “hit” by BLAST analyses are shown. Where applicable, we also show number of GO terms mapped by the GeneIDs that got hit and number GeneIDs among this list that have a mapped GO term.</p