Performance Analysis of Best Relaying Protocol Selection with Interferences at Relays

In this paper, we investigate the performance of selecting the best protocol between amplify and forward (AF) and decode and forward (DF) in multiple relay networks with multiple interferences at relays. In the selection scheme, the best protocol between AF and DF is selected depending on the comparisons of signal-to-interference and noise ratio (SINR) for all source-relay links. All relays measure the received SINR to decide forwarding signal or not. When SINR is above a certain threshold then DF is used otherwise AF is used. Particularly, we develop an accurate mathematical model for best relaying protocol by considering the effect of interferences to our scheme. Firstly, we derive the asymptotic closed form expression for the symbol error rate (SER) for the system under study. Also we derive an upper and lower bound of symbol error rate and show how they were tight with exact SER. Furthermore an approximate expression for the outage probability is derived. Numerical results are finally presented to validate the theoretical analysis with a different number of relays

Photonic circuits for generating modal, spectral, and polarization entanglement

We consider the design of photonic circuits that make use of Ti:LiNbO$_{3}$ diffused channel waveguides for generating photons with various combinations of modal, spectral, and polarization entanglement. Down-converted photon pairs are generated via spontaneous optical parametric down-conversion (SPDC) in a two-mode waveguide. We study a class of photonic circuits comprising: 1) a nonlinear periodically poled two-mode waveguide structure, 2) a set of single-mode and two-mode waveguide-based couplers arranged in such a way that they suitably separate the three photons comprising the SPDC process, and, for some applications, 3) a holographic Bragg grating that acts as a dichroic reflector. The first circuit produces frequency-degenerate down-converted photons, each with even spatial parity, in two separate single-mode waveguides. Changing the parameters of the elements allows this same circuit to produce two nondegenerate down-converted photons that are entangled in frequency or simultaneously entangled in frequency and polarization. The second photonic circuit is designed to produce modal entanglement by distinguishing the photons on the basis of their frequencies. A modified version of this circuit can be used to generate photons that are doubly entangled in mode number and polarization. The third photonic circuit is designed to manage dispersion by converting modal, spectral, and polarization entanglement into path entanglement

A fluorophore attached to nicotinic acetylcholine receptor beta M2 detects productive binding of agonist to the alpha delta site

To study conformational transitions at the muscle nicotinic acetylcholine (ACh) receptor (nAChR), a rhodamine fluorophore was tethered to a Cys side chain introduced at the beta-19' position in the M2 region of the nAChR expressed in Xenopus oocytes. This procedure led to only minor changes in receptor function. During agonist application, fluorescence increased by (Delta-F/F) approximate to 10%, and the emission peak shifted to lower wavelengths, indicating a more hydrophobic environment for the fluorophore. The dose-response relations for Delta-F agreed well with those for epibatidine-induced currents, but were shifted approximate to 100-fold to the left of those for ACh-induced currents. Because (i) epibatidine binds more tightly to the alpha-gamma-binding site than to the alpha-delta site and (ii) ACh binds with reverse-site selectivity, these data suggest that Delta-F monitors an event linked to binding specifically at the alpha-delta-subunit interface. In experiments with flash-applied agonists, the earliest detectable Delta-F occurs within milliseconds, i.e., during activation. At low [ACh] (less than or equal to 10 muM), a phase of Delta-F occurs with the same time constant as desensitization, presumably monitoring an increased population of agonist-bound receptors. However, recovery from Delta-F is complete before the slowest phase of recovery from desensitization (time constant approximate to 250 s), showing that one or more desensitized states have fluorescence like that of the resting channel. That conformational transitions at the alpha-delta-binding site are not tightly coupled to channel activation suggests that sequential rather than fully concerted transitions occur during receptor gating. Thus, time-resolved fluorescence changes provide a powerful probe of nAChR conformational changes

The crystal structure of Pneumolysin at 2.0 Å resolution reveals the molecular packing of the pre-pore complex

Pneumolysin is a cholesterol-dependent cytolysin (CDC) and virulence factor of Streptococcus pneumoniae. It kills cells by forming pores assembled from oligomeric rings in cholesterol-containing membranes. Cryo-EM has revealed the structures of the membrane-surface bound pre-pore and inserted-pore oligomers, however the molecular contacts that mediate these oligomers are unknown because high-resolution information is not available. Here we have determined the crystal structure of full-length pneumolysin at 1.98 Å resolution. In the structure, crystal contacts demonstrate the likely interactions that enable polymerisation on the cell membrane and the molecular packing of the pre-pore complex. The hemolytic activity is abrogated in mutants that disrupt these intermolecular contacts, highlighting their importance during pore formation. An additional crystal structure of the membrane-binding domain alone suggests that changes in the conformation of a tryptophan rich-loop at the base of the toxin promote monomer-monomer interactions upon membrane binding by creating new contacts. Notably, residues at the interface are conserved in other members of the CDC family, suggesting a common mechanism for pore and pre-pore assembly