Influences of porphyromonas gingivalis on the viability of esophageal cancer cells.

A recent study demonstrated an association between the Porphyromonas gingivalis (Pg)infection and the progression of ESCC (Gao et al., 2016). However, how Pg infection affects the nature of ESCC remains unknown. We examined the effects of Pg on the proliferation and chemotherapy drug-induced apoptosis of ESCC cells and elucidated the signaling molecules involved in these processes. Method: Apoptosis and cell proliferation were tested using Flow cytometry and Tetrazolium salt based proliferation assays respectively. Western Blot was utilized to detect the protein expression. CDK2 and Cyclin E were knocked down in the ESCC cells through transfection. Unpaired T-test and ANOVA with post hoc Tuckey test were used to analyze relevant data (pResults: Pgenhanced the proliferation and reduced the chemotherapy drug-mediated apoptosis of ESCC cells possibly through Cyclin E-CDK2 pathway and Caspase-3 mediated signaling pathway, respectively. Conclusion: Pg worsens ESCC probably by promoting tumor growth and resisting chemotherapeutic drug-mediated apoptosis

Towards Comparative Analysis of Resumption Techniques in ETL

Data warehouses are loaded with data from sources such as operational data bases. Failure of loading process or failure of any of the process such as extraction or transformation is expensive because of the non-availability of data for analysis. With the advent of e-commerce and many real time application analysis of data in real time becomes a norm and hence any misses while the data is being loaded into data warehouse needs to be handled in an efficient and optimized way. The techniques to handle failure of process to populate the data are very much important as the actual loading process. Alternative arrangement needs to be made for in case of failure so that processes of populating the data warehouse are done in time. This paper explores the various ways through which a failed process of populating the data warehouse could be resumed. Various resumption techniques are compared and a novel block based technique is proposed to improve one of the existing resumption techniques

JAK3 restrains inflammatory responses and protects against periodontal disease through Wnt3a signaling

Homeostasis between pro- and anti- inflammatory responses induced by bacteria is critical for the maintenance of health. In the oral cavity, proinflammatory mechanisms induced by pathogenic bacteria are well-established; however, the anti-inflammatory responses that act to restrain innate responses remain poorly characterized. Here, we demonstrate that infection with the periodontal pathogen P. gingivalis enhances the activity of JAK3 in innate immune cells, and subsequently phospho-inactivates Nedd4-2, a ubiquitin E3 ligase. In turn, Wnt3 ubiquitination is decreased, while total protein levels are enhanced, leading to a reduction in proinflammatory cytokine levels. In contrast, JAK3 inhibition or Wnt3a robustly enhances NF-κB activity and the production of proinflammatory cytokines in P. gingivalis-stimulated innate immune cells. Moreover, using gain- and loss-of-function approaches, we demonstrate that downstream molecules of Wnt3a signaling, including Dvl3 and β-catenin, are responsible for the negative regulatory role of Wnt3a. In addition, using an in vivo P. gingivalis-mediated periodontal disease model, we show that JAK3 inhibition enhances infiltration of inflammatory cells, reduces expression of Wnt3a and Dvl3 in P. gingivalis-infected gingival tissues, and increases disease severity. Together, our results reveal a new anti-inflammatory role for JAK3 in innate immune cells and show that the underlying signaling pathway involves Nedd4-2-mediated Wnt3a ubiquitination

Internation Commercial Arbitration: The Need for Harmonized Legal Regime on Court-ordered Interim Measures of Relief

This thesis is an attempt to consider some of the challenges facing the regime of international commercial arbitration (ICA) in the contemporary global economy. It examines the governance mechanism of the regime of ICA in a globalizing economy. The thesis seeks to analyze the process of harmonization of the law of ICA with particular reference to availability of interim measures from court. In particular, the analytical focus is on how the globalizing economy affects the requirement of “court-ordered interim measures” in the arbitration process and how international arbitral regime attempts to cope with such changing demand of the globalizing economy. The thesis emphasizes the importance of harmonizing the national laws on the above-mentioned issue through ratifying international conventions as opposed to formulation of non-mandatory UNCITRAL Model Law.MAS

Evaluating Zn-Porphyrin-Based Near-IR-Sensitive Non-Fullerene Acceptors for Efficient Panchromatic Organic Solar Cells

Porphyrin‐based non‐fullerene acceptors (NFAs) have shown pronounced potential for assembling low‐bandgap materials with near‐infrared (NIR) characteristics. Herein, panchromatic‐type porphyrin‐based molecules (POR1–POR5) are proposed by modulating end‐capped acceptors of a highly efficient porphyrin‐based NFA PORTFIC(POR) for organic solar cells (OSCs). Quantum chemical structure‐property relationship has been studied to discover photovoltaic and optoelectronic characteristics of POR1–POR5. Results show that optoelectronic properties of the POR1–POR5 are better in all aspects when compared with the reference POR. All proposed NFAs particularly POR5 proved to be the preferable porphyrin‐based NIR sensitive NFA for OSCs applications owing to lower energy gap (1.56 eV), transition energy (1.11 eV), binding energy (E(b) =0.986 eV), electron mobility (λ(e)=0.007013E(h) ), hole mobility (λ(h) =0.004686 E(h)), high λ(max) =1116.27 nm and open‐circuit voltage (V(oc) =1.96 V) values in contrast to the reference POR and other proposed NFAs. This quantum chemical insight provides sufficient evidence about excellent potential of the proposed porphyrin‐based NIR sensitive NFA derivatives for their use in OSCs

Degradation of Vibrio cholerae from drinking water by the underwater capillary discharge

Underwater plasma discharge is considered a nontoxic and effectual purification approach to control waterborne bacterial pathogens. In the present study, Vibrio cholerae contaminated drinking water was sterilized by using underwater capillary discharge generation via high voltage, oxygen (O2{{\rm{O}}}_{2}) injection, and hydrogen peroxide (H2O2{{\rm{H}}}_{2}{{\rm{O}}}_{2}) addition. The effects of oxidant species generated by plasma discharge on V. cholerae disinfection have been studied and reported. The electrical and optical analysis of capillary discharge revealed the generation of reactive oxygen species (OH.{{\rm{OH}}}^{.}, H and O), which are highly useful for bacterial disinfection along with enhanced power and energy of discharge pulses. Complete elimination of V. cholerae (0 CFUs (colony forming units)) from the water after O2{{\rm{O}}}_{2} injected and H2O2{{\rm{H}}}_{2}{{\rm{O}}}_{2} added plasma discharge was achieved, and 100% inactivation of V. cholera from drinking water was proven. The retardant effect of the initial V. cholerae colonies with time variation was reported through optical density (OD600 nm) measurements. The time course study of bactericidal activity of plasma treatment on V. cholerae, observed every 12 h up to 36 h, revealed a high retardant effect on V. cholerae CFUs. Underwater capillary discharge is an efficient approach for the inactivation of V. cholerae from drinking water