Theory of Nucleosome Corkscrew Sliding in the Presence of Synthetic DNA Ligands

Histone octamers show a heat-induced mobility along DNA. Recent theoretical studies have established two mechanisms that are qualitatively and quantitatively compatible with in vitro experiments on nucleosome sliding: Octamer repositiong through one-basepair twist defects and through ten-basepair bulge defects. A recent experiment demonstrated that the repositioning is strongly suppressed in the presence of minor-groove binding DNA ligands. In the present study we give a quantitative theory for nucleosome repositioning in the presence of such ligands. We show that the experimentally observed octamer mobilities are consistent with the picture of bound ligands blocking the passage of twist defects through the nucleosome. This strongly supports the model of twist defects inducing a corkscrew motion of the nucleosome as the underlying mechanism of nucleosome sliding. We provide a theoretical estimate of the nucleosomal mobility without adjustable parameters, as a function of ligand concentration, binding affinity, binding site orientiation, temperature and DNA anisotropy. Having this mobility at hand we speculate about the interaction between a nucleosome and a transcribing RNA polymerase and suggest a novel mechanism that might account for polymerase induced nucleosome repositioning.Comment: 23 pages, 4 figures, submitted to J. Mol. Bio

Job Satisfaction of the Employees in the Mobile Phone Corporates in Bangladesh: A Case Study

Optimizing employee satisfaction is a key to the success of any business that relies on a variety of organizational and psycho-economic factors. This study was conducted to identify that sort of key factors, which are responsible to influence on the overall job satisfaction in the growing mobile phone corporate in Bangladesh. The phone corporates, which are included here in the study, are Grameen Phone (GP), Bangla Link and Aktel. The factors included in the investigation as independent variables are Compensation Package, Supervision, Career Growth, Training and Development, Working atmosphere, Company Loyalty and Performance Appraisal. The result indicates that training and performance appraisal, work atmosphere, compensation package, supervision, and company loyalty are the key factors that impact on employees’ job satisfaction in these corporations. The study also finds that the employees of these three corporations possessed above of the moderate level and positive attitude towards job satisfaction, which could be nudged up to excellent status of employee satisfaction if the management takes those identified factors with a little more rigorous weight into their considerations and acts further accordingly.

Strategic Communications and Corporate Branding: a Study of Jebsen & Jessen Indonesia Group

Corporate communications hold a vital function to maintain the company's positive image and reputation in front of stakeholders. A corporate communications practitioner must be able to handle a variety of communication processes in an organization, including to determine the right strategy to reach its communications goal. The purpose of this research is to analyze the corporate communications strategy of Jebsen & Jessen Indonesia Group to create its corporate branding and reflect it with public relations strategic planning process. The researcher gathered the primary data from in-depth interview with key informant and combine it with secondary data from other relevant data. Afterwards, it was verified by triangulation to strengthen the credibility of data and continue with analysis process. This research concluded that Jebsen & Jessen Indonesia Group has implemented its corporate branding strategy accordingly to public relations strategic planning process. Some of the targets have been achieved in one year period, however there have been suggestions to improve the future corporate branding strategy formation

What influences the Changes in REIT CEO Compensation?: Evidence from Panel Data

This study examines what influences the changes in REIT CEO compensation using the following performance measures: average three-year total returns to shareholders, market value added, Tobin's q, and change in funds from operations. In addition, we examine the impact of managerial power on the change in compensation. Unbalanced panel data is employed to capture both the time-series and cross-sectional effects. The empirical evidence indicates that firm performance and size do not influence the change in CEO salary, while risk, tenure, title, ownership, and age have significant impacts. Contrary to previous findings and a priori expectations, bonuses are not influenced by risk, size, or CEO power; however, they are influenced by performance. Option awards are affected by performance and CEO power.

Modification of as-cast Al-Mg/B4C composite by addition of Zr

Zirconium was used in Al-Mg/B4C composite to improve compocasting efficiency by increasing particle incorporation. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) results revealed that by addition of zirconium a reaction layer containing Zr, Al, B and C is formed on the interface of B4C-matrix. X-ray diffraction (XRD) analysis of extracted particles unveiled that the ZrB2 phase is the main constituent of this layer. Formation of ZrB2 is an exothermic reaction which can rise temperature locally around particles and agglomerates. Rising temperature around agglomerates in conjunction with turbulent flow of melt facilitates agglomerates wetting and dissolving into molten aluminum. As the result, final product contains more uniformly distributed B4C particles. Besides enhancing compocasting efficiency, addition of Zr and formation of reaction layer by improving particle matrix bonding quality, led to increase in ultimate tensile strength and elongation of the composite around 8% and 30%, respectively. SEM observations of the fracture surfaces confirmed that a proper bonding presents at the interface of particles and matrix in presence of Zr.Peer ReviewedPostprint (author's final draft

Synthesis ,Characterization and invitro evaluation of anticancer activity of new hydroxamicacid basedHDACi containing substituted thaizolesas a cap linking moiety.

The present study was undertaken to synthesize, characterize  and evaluate the anticancer activity of  new derivatives of hydroxamate –based HDACi  having S-subistituted-5-amino1,3,4thadiazole as a cap linking moiety ,with suitable aliphatic linker.The structures and purity of the targeted compounds were confirmed by TLC , FTIR ,H-NMR and mass spectroscopy and their anticancer activity were evaluated by comparative cytotoxic study , Using HeLa nuclear extract and normal embryonic fibroblasts cell lines.All the synthesized compounds shows good anticancer activity, represented by their high rate of growth inhibition on Hela cell line and low cytotocic effect on normal cell line .Compound (VAb) show the best safety index(SI) that represented by its  selective cytotoxic activity on HeLa cell linewith  low cytotoxic effect on normal embryonic cell line