Frequency of Latent Autoimmune Diabetes in Adults (LADA) in Phenotypically Type2 Diabetic Patients in Qom

Background: Latent autoimmune diabetes of adults (LADA) is a slowly progressive form of diabetes managed with diet and oral hypoglycemic agents before becoming insulin requiring. Islet autoantibodies are used for differential diagnosis between LADA and Type 2 diabetes mellitus. The aims of this study were to investigate the frequency of LADA using islet autontibodies in population with T2DM and characterize clinical and biochemical characteristics of them. Materials and methods: In this cross-sectional study, 86 T2DM patients aged between 28-67 years were tested for the presence of autoantibodies by ELISA technique. The Glutamic acid decarboxylase antibody, Islet Cell Antibody and Insulinoma Antigen2 Antibody were evaluated for diagnosis of LADA patients. Results: Of 86 T2DM, 35 (40.70%) were antibody positive and 51 (59.30%) persons were negative for any antibody. A significant difference was found between groups in age at study, insulin therapy, C- peptide levels and family history of autoimmune diseases. On the other hand, no significant difference was found between the two groups in other risk factors. Conclusions: Among our T2DM subjects, the number of LADA patients were not high. Also, multiple autoantibodies were more frequent than single autoantibodies in LADA patient

Atefeh Khazeni, Peter H. Adler, Zakieh Telmadareiiy, Mohammad Ali Oshaghi1,*, Hasan Vatandoost, Seyed Mohammad Abtahi & Abolfazl Lotfi

Khazeni, Atefeh, Adler, Peter H., Telmadareiiy, Zakieh, Oshaghi, Mohammad Ali, Vatandoost, Hasan, Abtahi, Seyed Mohammad, Lotfi, Abolfazl (2013): Atefeh Khazeni, Peter H. Adler, Zakieh Telmadareiiy, Mohammad Ali Oshaghi1,*, Hasan Vatandoost, Seyed Mohammad Abtahi & Abolfazl Lotfi. Zootaxa 3718 (6): 600-600, DOI: http://dx.doi.org/10.11646/zootaxa.3718.6.

FIGURE 1 in The Black Flies (Diptera: Simuliidae) of Iran

FIGURE 1. Map of Iran showing ecoregions recognized by the World Wildlife Federation (2013b) and collection sites 1 to 16 listed in Table 1. A = Central Persian Desert Basins. B = Arabian Desert and East Sahero-Arabian Xeric Shrublands. C = Kopet Dag Woodlands and Forest Steppe. D = Registan-North Pakistan Sandy Desert. E = Caspian Lowland Desert. F = Caspian Hyrcanian Mixed Forests. G = Elburz Range Forest Steppe. H = Zagros Mountains Forest Steppe. I = South Iran-Nubo Sindian Desert and Semi-Desert. J = Tigris-Euphrates Alluvial Salt Marsh. K = Kopet-Dag Semi-Desert. L = Azerbaijan Shrub Desert and Steppe. M = Eastern Anatolian Montane Steppe

Metacnephia

Genus METACNEPHIA Crosskey 1. Metacnephia persica (Rubtsov) This species is one of five formally described from Iran (Rubtsov 1940). It is known from only one definitive location in the country, the type locality in Tabriz, Tehran Province (Crosskey 2002), in the Eastern Anatolian Montane Steppe ecoregion. Genus SIMULIUM Latreille Subgenus BYSSODON Enderlein GRISEICOLLE species-group Subgenus CROSSKEYELLUM Grenier & Bailly-Choumara 3. Simulium (Crosskeyellum) zagros (Yankovsky) This species is known from a single female described by Yankovsky (2010) from Lorestan Province. Its occurrence in Iran is oddly disjunct from the only other species in the subgenus Crosskeyellum, which inhabits Morocco. The type of S. zagros, therefore, requires restudy to test its current subgeneric assignment. Subgenus EUSIMULIUM Roubaud The following two members of the S. aureum group previously have been identified morphologically from Iran: 4. Simulium (Eusimulium) brachyantherum Rubtsov A series of larvae, pupae, males, and females was examined from the Elburz Mountains by Crosskey (2002) and assigned tentatively to S. brachyantherum. The record falls within the Elburz Range Forest Steppe ecoregion. Simulium (Eusimulium) morphoforms Our larval collections in ethanol could not be analyzed chromosomally and, therefore, are recorded here to the level of subgenus. New records: Site 2 (2 larvae on 4 Sept 2010; 1 larva on 5 Nov 2010), Site 5 (8 larvae on 21 March 2011), Site 9 (3 larvae on 28 March 2012), Site 11 (5 larvae on 12 May 2012), Site 12 (2 larvae on 27 April 2012). Simulium (Eusimulium) cytoforms We found two chromosomally distinct species of the S. aureum group, neither of which has been reported previously in the literature and neither of which could yet be tied to a nominal species. One of these species, based on a larva taken near Tehran (Site 2, 5 Nov 2010), is chromosomally similar to Simulium (Eusimulium) velutinum (Santos Abreu), but had 2 additional homozygous inversions in IS. Whether the larva represents true S. velutinum or a closely related species must await further sampling. The second species, which previously had been found in Armenia (Adler, unpublished), is based on 3 larvae collected from North Khorasan Province (Site 11, 12 May 2012). These larvae had unique sequences in all 4 arms, but to determine if some of the inverted sequences represent polymorphisms, rather than fixed inversions, requires additional sampling. Subgenus MONTISIMULIUM Rubtsov Subgenus NEVERMANNIA Enderlein RUFICORNE species-group 7. Simulium (Nevermannia) brevitarse (Rubtsov) The larvae of this species have not been described, but our identifications of larvae were based on the similarity of the pupal gill histoblasts to published descriptions of the pupal gill. The only previous record of this species in Iran is from the Elburz Mountains (Crosskey 2002). Our collections of larvae from two distinct ecoregions suggest the possibility of multiple species. New records: Site 11 (4 larvae on 12 May 2012), Site 13 (6 larvae on 25 Sept 2012). 8. Simulium (Nevermannia) crassicaulum (Rubtsov) We record this species, previously known only from Uzbekistan, for the first time from Iran (Lorestan Province). New record: Site 13 (1 larva and 2 pupae on 25 Sept 2012). Subgenus SIMULIUM Latreille BEZZII species-group 9. Simulium (Simulium) alajense Rubtsov We identified this species chromosomally, based on the marked similarity with chromosomal material studied by Adler (unpublished) in Mongolia more than 3500 km to the northeast. In the absence of associated adults from either country, the name ‘ alajense ’ is linked tentatively with the material, which actually might represent one of the other members of the S. bezzii species-group, such as S. coarctatum. New record: Site 7 (18 larvae on 14 May 2012). 10. Simulium (Simulium) bezzii (Corti) Simulium bezzii, previously known in Iran from a single collection 26 km northeast of Tehran (Crosskey 2002) was common in our samples. Our chromosomal examination of larvae indicated the presence of at least two cytological forms, based on inversion differences in the long arm of chromosome III, one cytoform across northern Iran and the other in Doaab (Site 13). New records: Site 1 (20 larvae and 10 pupae on 28 Feb 2012; 3 larvae on 14 October 2011), Site 2 (33 larvae on 4 Sept 2010; 50 larvae on 5 Nov 2010), Site 3 (3 larvae on 7 Sept 2010; 6 larvae on 9 Nov 2010), Site 7 (2 larvae on 29 Feb 2012; 19 larvae on 27 March 2012; 2 larvae on 14 May 2012), Site 9 (5 larvae on 28 March 2012; 45 larvae on 16 May 2012), Site 10 (16 larvae on 18 April 2012), Site 11 (33 larvae on 12 May 2012), Site 12 (5 larvae on 27 April 2012), Site 13 (7 larvae on 25 Sept 2012), Site 15 (20 larvae on 18 March 2011). 11. Simulium (Simulium) coarctatum Rubtsov Yankovsky (2010) identified a single female of S. coarctatum from Lorestan Province. 12. Simulium (Simulium) iranicum (Yankovsky) This species was described from 10 females collected in Lorestan Province (Yankovsky 2010). ORNATUM species-group Along with S. bezzii and the subgenus Wilhelmia, the S. ornatum species-group was the most commonly represented taxon in our collections. 13. Simulium (Simulium) kiritshenkoi Rubtsov As a member of the chromosomally diverse and morphologically homogeneous S. ornatum group, the identification of S. kiritshenkoi is problematic. The type (female) of S. kiritshenkoi was collected in the Shahrud area of northern Iran on 26 May 1944. We examined the chromosomes of larvae from North Khorasan and Tehran Provinces, within 250-300 km both east and west of Shahrud. These samples were chromosomally cohesive, suggesting that they represent the type specimen and can be considered bona fide S. kiritshenkoi. We also tentatively consider a population in Doaab (Alashtar; Site 13) to be S. kiritshenkoi, although it is chromosomally different from populations in northern Iran. Crosskey (2002) provided additional, morphologically based records for S. kiritshenkoi. New records: Site 2 (6 larvae on 4 Sept 2010; 9 larvae on 5 Nov 2010), Site 3 (1 larva on 7 Sept 2010), Site 6? (3 larvae on 23 Feb 2012), Site 7 (2 larvae on 29 Feb 2012; 17 larvae on 27 March 2012; 10 larvae on 14 May 2012), Site 8 (6 larvae on 24 Feb 2012), Site 9 (45 larvae on 28 March 2012; 45 larvae on 16 May 2012), Site 11 (24 larvae on 12 May 2012), Site 12 (50 larvae on 27 April 2012), Site 13 (20 larvae on 25 Sept 2012), Site 15 (1 larva on 18 March 2011; 1 larva on 22 Aug 2011), Site 16 (200 larvae on 10 Aug 2012). 14. Simulium (Simulium) fontanum Terteryan The only known record of this species in Iran—a single male with its pupal exuviae—is from the Elburz Mountains (Crosskey 2002). The identity of this species needs to be resolved chromosomally vis à vis other Asian members of the S. ornatum group. VARIEGATUM species-group 15. Simulium (Simulium) variegatum Meigen Crosskey (2002) provided the first record of S. variegatum for Iran, a single collection of larvae, pupae, females, and males from the Elburz Mountains. We provide a chromosomally verified record from Doaab (Site 13), based on a female larva with the basic banding sequence characteristic of S. variegatum. New records: Site 3 (5 larvae on 9 Nov 2010), Site 8? (7 small larvae on 26 March 2012), Site 13 (1 larva on 25 Sept 2012). Subgenus TRICHODAGMIA Enderlein ALBELLUM species-group 16. Simulium (Trichodagmia) margaritae (Rubtsov) The Iranian record for this species comes from a veterinary report of biting problems on cattle in the Arasbaran area of northwestern Iran (Nikdel et al. 2003). 17. Simulium (Trichodagmia) transcaspicum Enderlein Previous Iranian records of S. transcaspicum in Iran are of adults from the Elburz Mountains and Caspian Sea coast (Crosskey 2002). We provide the world’s southernmost record for this species. Our collection was based only on larvae. Additional life stages from this area should be studied to determine if the material is conspecific with the type from Turkmenistan. New record: Site 14 (40 larvae on 15 April 2012). Subgenus WILHELMIA Enderlein EQUINUM species-group 18. Simulium (Wilhelmia) lineatum (Meigen) Simulium lineatum is at the southeastern extreme of its distribution in Iran. All previous records, except one from Tabriz (ca. 80 km south of the Armenian border), are from the Isfahan area (Crosskey 2002). The pupae are the most easily identified of the Wilhelmia species in Iran. The identity of our larvae from the Zayandeh River in the Isfahan area (Site 4) was confirmed chromosomally. New records: Site 4 (34 larvae on 26 March 2011), Site 10 (2 larvae and 1 pupa on 18 April 2012). 19. Simulium (Wilhelmia) lurestanicum (Yankovsky) Simulium lurestanicum was described by Yankovsky (2010) from a series of females collected in Lorestan Province. The size and shape of the spermatheca is diagnostic for females of the subgenus Wilhelmia in Iran (Crosskey 2002). Given the size and shape of the spermatheca illustrated by Yankovsky (2010), and the lack of additional convincing diagnostic characters, we suspect that S. lurestanicum is a synonym of S. paraequinum. Pending study of the type series, we refrain from proposing formal synonymy. 20. Simulium (Wilhelmia) paraequinum Puri One previous record for S. paraequinum in Iran, based on a single male from Chalous on the Caspian Sea coast, was reported (Crosskey 2002). The larvae and pupae can be difficult to distinguish morphologically from those of S. pseudequinum, and our material here is recorded as S. paraequinum / pseudequinum, unless a chromosomal identification was made. New records (for S. paraequinum / pseudequinum): Site 1 (12 larvae on 28 Feb 2012), Site 5 (2 larvae on 21 March 2011), Site 6 (1 larva on 23 Feb 2012), Site 7 (15 larvae on 29 Feb 2012; 30 larvae on 27 March 2012; 19 larvae on 14 May 2012), Site 8 (2 larvae on 26 March 2012), Site 9 (1 larva on 28 March 2012), Site 13 (200 larvae on 25 Sept 2012), Site 14 (3 larvae on 15 April 2012). 21. Simulium (Wilhelmia) pseudequinum Séguy The only previous record (Shahrud) of S. pseudequinum in Iran was given by Rubtsov (1951; as Wilhelmia mediterranea). We chromosomally identified this species from the Zayandeh River in the Isfahan area (Site 4), which also allowed us to distinguish the immature larvae from those of S. lineatum in the same collection. The chromosomes of S. pseudequinum, unlike those of S. paraequinum and S. lineatum, lack the chromocentric-like arrangement of the centromeres (Chubareva & Petrova 2008). New record: Site 4 (51 larvae on 26 March 2011). 22. Simulium (Wilhelmia) veltistshevi Rubtsov The record of S. veltistshevi in Iran is uncertain. It appeared with a query mark in the world inventory of Crosskey & Howard (1997), and has been carried through subsequent revisions of the inventory. Its occurrence in Iran, however, requires verification. Genus SULCICNEPHIA Rubtsov 23. Sulcicnephia znoikoi (Rubtsov) Crosskey (2002) provided the only record (Sarab) of this species from Iran.Published as part of Khazeni, Atefeh, Adler, Peter H., Telmadareiiy, Zakieh, Oshaghi, Mohammad Ali, Vatandoost, Hasan, Abtahi, Seyed Mohammad & Lotfi, Abolfazl, 2013, The Black Flies (Diptera: Simuliidae) of Iran, pp. 67-74 in Zootaxa 3694 (1) on pages 68-72, DOI: 10.11646/zootaxa.3694.1.5, http://zenodo.org/record/28432

Assessment of the Prevalence of Hepatitis C and B Viruses in Patients with Hemophilia in Qom Province, (Iran)

Background and Objectives: Hemophilia is a hereditary bleeding disorder, which CFC (clotting factor concentration) method is used for prevention and treatment of about 70% of these patients. This method can play an important role in the transmission of blood-borne viruses, such as hepatitis B and C. According to studies, more than 40% of patients with hemophilia have one of the hepatitis C, B, and D, or HIV. The objective of this study was to determine prevalence of hepatitis B and C in the hemophilia population of Qom province.   Methods: This analytical cross-sectional study, was carried out on 90 patients with hemophilia Qom province, in 2017. Primary diagnosis and screening of hepatitis, were performed using serology for HBV and HCV; then, the samples were analyzed by PCR method. The data were statistically analyzed by Chi-square test.   Results: In this study, 70 subjects of the statistical population were male and 20 subjects were female, and their mean age was 27 years. In the serological survey, anti HCV, HBsAg, and HBcAb, were positive in 27.8%, 0%, and 11.1%, respectively. Moreover, the results of the PCR test for HBV was negative and for HCV was positive in 9 out of 25 individuals, who were anti-HCV positive.   Conclusion: The results of this study showed that among the blood-borne viral hepatitis in the hemophilia population, the incidence of hepatitis C is higher. Also, in recent years, monitoring the donated blood reduced the risk of the incidence of viral hepatitis in the blood recipient hemophilic populations. Accordingly, continuous testing and paying attention to the vaccination schedule seems to be necessary in this group of patients

MiRNA-related metastasis in oral cancer: moving and shaking

Abstract Across the world, oral cancer is a prevalent tumor. Over the years, both its mortality and incidence have grown. Oral cancer metastasis is a complex process involving cell invasion, migration, proliferation, and egress from cancer tissue either by lymphatic vessels or blood vessels. MicroRNAs (miRNAs) are essential short non-coding RNAs, which can act either as tumor suppressors or as oncogenes to control cancer development. Cancer metastasis is a multi-step process, in which miRNAs can inhibit or stimulate metastasis at all stages, including epithelial-mesenchymal transition, migration, invasion, and colonization, by targeting critical genes in these pathways. On the other hand, long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), two different types of non-coding RNAs, can regulate cancer metastasis by affecting gene expression through cross-talk with miRNAs. We reviewed the scientific literature (Google Scholar, Scopus, and PubMed) for the period 2000–2023 to find reports concerning miRNAs and lncRNA/circRNA-miRNA-mRNA networks, which control the spread of oral cancer cells by affecting invasion, migration, and metastasis. According to these reports, miRNAs are involved in the regulation of metastasis pathways either by directly or indirectly targeting genes associated with metastasis. Moreover, circRNAs and lncRNAs can induce or suppress oral cancer metastasis by acting as competing endogenous RNAs to inhibit the effect of miRNA suppression on specific mRNAs. Overall, non-coding RNAs (especially miRNAs) could help to create innovative therapeutic methods for the control of oral cancer metastases